Esthetic and function rehabilitation of severely worn dentition with prosthetic-restorative approach and VDO increase. Case report

The aim of this study is to report a case of restorative treatment of severely worn dentition. In this case report, the erosive/abrasive worn dentition have been reconstructed with metal ceramic crown on the posterior teeth and with ceramic veneers on the anterior teeth 1.3 - 2.3 and 3.4 - 4.3. A prosthetic treatment was proposed to a male patient of 58 years old having a clinically significant tooth wear. After clinical exam, impressions of maxillary and mandible arches were taken with alginate to obtain preliminary casts for diagnostic waxing to all maxillary and mandibular teeth and fabrication of all provisional crowns in acrylic resin for posterior teeth, and from the diagnostic wax-up were fabricated a silicone guide masks for anterior teeth. An increase in VDO should be determined on the basis of a need to accomplish satisfactory and aesthetically pleasing restorations; it was proposed to increase the incisal lenght of the maxillary anterior incisors, together with alteration of the VDO 3 mm anteriorly. The posterior teeth 1.6 - 1.5 - 1.4 - 2.4 - 2.6 - 3.5 - 3.6 - 3.7 - 4.4 - 4.7, where the amount of tissue lost was greater, were recontructed with metal ceramic crowns. Two implants (Nobel replace 4.3x10) was placed. The implant were located in the area 4.5 - 4.6. The anterior teeth were restored with veneers. The prosthetic challenge with restoring severely worn dentitions is to preserve as much of the already diminished tooth structure as possible for retention while also providing enough interocclusal space for the restorative material

Mapping genetic determinants of host susceptibility to Pseudomonas aeruginosa lung infection in mice.

Background: P. aeruginosa is one of the top three causes of opportunistic human bacterial infections. The remarkable variability in the clinical outcomes of this infection is thought to be associated with genetic predisposition. However, the genes underlying host susceptibility to P. aeruginosa infection are still largely unknown. Results: As a step towards mapping these genes, we applied a genome wide linkage analysis approach to a mouse model. A large F2 intercross population, obtained by mating P. aeruginosa-resistant C3H/HeOuJ, and susceptible A/J mice, was used for quantitative trait locus (QTL) mapping. The F2 progenies were challenged with a P. aeruginosa clinical strain and monitored for the survival time up to 7 days post-infection, as a disease phenotype associated trait. Selected phenotypic extremes of the F2 distribution were genotyped with high-density single nucleotide polymorphic (SNP) markers, and subsequently QTL analysis was performed. A significant locus was mapped on chromosome 6 and was named P. aeruginosa infection resistance locus 1 (Pairl1). The most promising candidate genes, including Dok1, Tacr1, Cd207, Clec4f, Gp9, Gata2, Foxp1, are related to pathogen sensing, neutrophils and macrophages recruitment and inflammatory processes. Conclusions: We propose a set of genes involved in the pathogenesis of P. aeruginosa infection that may be explored to complement human studie

Tracking the immunopathological response to Pseudomonas aeruginosa during respiratory infections

Repeated cycles of infections, caused mainly by Pseudomonas aeruginosa, combined with a robust host immune response and tissue injury, determine the course and outcome of cystic fibrosis (CF) lung disease. As the disease progresses, P. aeruginosa adapts to the host modifying dramatically its phenotype; however, it remains unclear whether and how bacterial adaptive variants and their persistence influence the pathogenesis and disease development. Using in vitro and murine models of infection, we showed that P. aeruginosa CF-adaptive variants shaped the innate immune response favoring their persistence. Next, we refined a murine model of chronic pneumonia extending P. aeruginosa infection up to three months. In this model, including CFTR-deficient mice, we unveil that the P. aeruginosa persistence lead to CF hallmarks of airway remodelling and fibrosis, including epithelial hyperplasia and structure degeneration, goblet cell metaplasia, collagen deposition, elastin degradation and several additional markers of tissue damage. This murine model of P. aeruginosa chronic infection, reproducing CF lung pathology, will be instrumental to identify novel molecular targets and test newly tailored molecules inhibiting chronic inflammation and tissue damage processes in pre-clinical studies

IL-17A impairs host tolerance during airway chronic infection by Pseudomonas aeruginosa

Resistance and tolerance mechanisms participate to the interplay between host and pathogens. IL-17-mediated response has been shown to be crucial for host resistance to respiratory infections, whereas its role in host tolerance during chronic airway colonization is still unclear. Here, we investigated whether IL-17-mediated response modulates mechanisms of host tolerance during airways chronic infection by P. aeruginosa. First, we found that IL-17A levels were sustained in mice at both early and advanced stages of P. aeruginosa chronic infection and confirmed these observations in human respiratory samples from cystic fibrosis patients infected by P. aeruginosa. Using IL-17a(-/-) or IL-17ra(-/-) mice, we found that the deficiency of IL-17A/IL-17RA axis was associated with: i) increased incidence of chronic infection and bacterial burden, indicating its role in the host resistance to P. aeruginosa; ii) reduced cytokine levels (KC), tissue innate immune cells and markers of tissue damage (pro-MMP-9, elastin degradation, TGF-β1), proving alteration of host tolerance. Blockade of IL-17A activity by a monoclonal antibody, started when chronic infection is established, did not alter host resistance but increased tolerance. In conclusion, this study identifies IL-17-mediated response as a negative regulator of host tolerance during P. aeruginosa chronic airway infection

STARTUP - L’ANALISI MULTICRITERIA, ALGORITMO DI UN’APPLICAZIONE SOFTWARE

UniverCityappeal is a web platform and a software application that is designed to help students in the choice of the university in which to continue their studies. Service: a database of certified parameters regarding academic departments (quality, employment rate postgraduate, university fees) and the host cities (cost of living, quality of services and mobility). The idea of business by innovative, at the base of the web platform and application software is the elaboration of an algorithm for comparing more data that leads to an index of alternatives for each user. DOI: http://dx.medra.org/10.19254/LaborEst.11.0

Il dilemma educativo di un reazionario: La Théorie de l’éducation sociale di Louis de Bonald

The article intends to present one of the lesser known works of the reactionary thinker Louis de Bonald, the Théorie de l’éducation sociale. What emerges is an educational concept that isn't always coherent, but interesting as it's paradigmatic of a troubled era in European history.L'articolo intende presentare una delle opere meno note del pensatore reazionario Louis de Bonald, la Théorie de l’éducation sociale. Ne emerge una concezione educativa non sempre coerente, ma interessante poiché paradigmatica di un'epoca tormentata della storia europea

Date Marks, Valuation, and Food Waste: Three In-Store ‘Eggsperiments’

We provide causal evidence on how date marking policies influence consumers' valuation of perishable food products through three consecutive research steps. In a preparatory in-store survey (n = 100), we identify perishable food items that can be experimentally manipulated to overcome core challenges for causal identification. A modified in-store multiple price list (MPL) experiment (n = 200) then tests consumers' valuation of perishable food of varying shelf-life (expiry date) in a two-by-two design that varies date mark type(use-by versus best-before) and information status while preventing free disposal censoring. We find that expiry dates affect consumer valuation, but not differences in date mark type. Educating consumers about date mark meaning turns out to be conducive to discarding potentially unsafe food, but not to preventing food waste. An attentiveness experiment (n = 160) tests whether these treatment effects plausibly result from the nature of consumers' knowledge and finds that the existing asymmetry in consumers' understanding of current date marks can explain the evidence from the modified MPL experiment

La riflessione di Guardini sul valore formativo degli studi accademici: i Tre scritti sull’università

The article analyzes the cues for thought about the educational and trainingaims of the university offered by Guardini’s Tre scritti sull’università. It containsthe theme, dear to the author, of the difficult relationship between anauthentically human and technical dimension, carried out according to a perspectivethat places the university institution at the center, called to indicatethe path that leads to the eternal truth on which form of knowledge is based:God.L’articolo analizza gli spunti di riflessione sulle finalità educative e formativedell’università offerti dall’opera di Guardini Tre scritti sull’università. In essasi ritrova il tema, caro all’Autore, del difficile rapporto tra dimensione autenticamenteumana e tecnica, svolto secondo una prospettiva che pone al centrol’istituzione universitaria, chiamata ad indicare la via che conduce allaverità eterna su cui si fonda ogni forma di conoscenza: Dio

Dampening Host Sensing and Avoiding Recognition in Pseudomonas aeruginosa Pneumonia

Pseudomonas aeruginosa is an opportunistic pathogen and causes a wide range of acute and chronic infections. P. aeruginosa infections are kept in check by an effective immune surveillance in the healthy host, while any imbalance or defect in the normal immune response can manifest in disease. Invasive acute infection in the immunocompromised patients is mediated by potent extracellular and cell bound bacterial virulence factors. Life-threatening chronic infection in cystic fibrosis patients is maintained by pathogenic variants that contribute to evade detection and clearance by the immune system. Here, we reviewed the molecular basis of receptor-mediated recognition of P. aeruginosa and their role in initiating inflammation and the colonization. In addition, the consequence of the P. aeruginosa genetic adaptation for the antibacterial defence and the maintaining of chronic infection are discussed