Organotypic tissue culture investigation of homocysteine thiolactone cardiotoxic effect

Homocysteine thiolactone was demonstrated to inhibit the growth of 10–12-day-old chicken embryo cardiac tissue explants at 7 × 10−9 −1 × 10−3 M concentrations in a dose-dependent manner. The maximal cardiotoxic effect of homocysteine thiolactone was detected at 1 × 10−3 M, which corresponds to severe hyperhomocysteinemia. The results of experiments on culturing of cardiac tissue explants in the medium containing homocysteine thiolactone (1 × 10−3 M) and ouabain at concentrations regulating the signal-transducing (1 × 10−10 M) and pumping (1 × 10−8 M) functions of Na+,K+ -ATPase indicate that the cardiotoxic effect of homocysteine thiolactone is supposed to result from inhibition of the Na+,K+ -ATPase pumping function

Development and Research of Technology Manufacturing of Semi-Finished Products from Solder Alloys Based on Silver

Proposed new compositions of solder alloys based on silver. Developed technological schemes of production of wire diameter 1,2 mm. Developed scheme of production of wire diameter 1,2 mm from alloy SAg-40I with operation combined casting, rolling and extruding. Modeling of temperature-rate parameters of the process of combined casting, rolling and extruding has been made. Designed and constructed the installation CCRE-80. The analysis of the structure and properties of semi-finished solder alloys based on silver has been done.Предложены новые составы припойных сплавов на основе серебра. Разработаны технологические схемы производства проволоки диаметром 1,2 мм. Разработана схема производства проволоки диаметром 1,2 мм из сплава ПСр-40И с применением операции совмещенного литья, прокатки и прессования. Проведено моделирование температурно- скоростных параметров процесса совмещенного литья, прокатки и прессования. Спроектирована и изготовлена установка СЛИПП-80. Проведен анализ структуры и свойств полуфабрикатов из припойных сплавов на основе серебра

Association between antiarrhythmic, electrophysiological and antioxidative effects of melatonin in ischemia/reperfusion

Melatonin is assumed to confer cardioprotective action via antioxidative properties. We evaluated the association between ventricular tachycardia and/or ventricular fibrillation (VT/VF) incidence, oxidative stress, and myocardial electrophysiological parameters in experimental ischemia/reperfusion under melatonin treatment. Melatonin was given to 28 rats (10 mg/kg/day, orally, for 7 days) and 13 animals received placebo. In the anesthetized animals, coronary occlusion was induced for 5 min followed by reperfusion with recording of unipolar electrograms from ventricular epicardium with a 64-lead array. Effects of melatonin on transmembrane potentials were studied in ventricular preparations of 7 rats in normal and ?ischemic? conditions. Melatonin treatment was associated with lower VT/VF incidence at reperfusion, shorter baseline activation times (ATs), and activation-repolarization intervals and more complete recovery of repolarization times (RTs) at reperfusion (less baseline-reperfusion difference, ΔRT) (p < 0.05). Superoxide dismutase (SOD) activity was higher in the treated animals and associated with ΔRT (p = 0.001), whereas VT/VF incidence was associated with baseline ATs (p = 0.020). In vitro, melatonin led to a more complete restoration of action potential durations and resting membrane potentials at reoxygenation (p < 0.05). Thus, the antioxidative properties of melatonin were associated with its influence on repolarization duration, whereas the melatonin-related antiarrhythmic effect was associated with its oxidative stress-independent action on ventricular activation.Fil: Sedova, Ksenia A.. Czech Technical University In Prague; República ChecaFil: Bernikova, Olesya G.. Ural Branch of Russian Academy of Sciences; RusiaFil: Cuprova, Julia I.. Czech Technical University In Prague; República ChecaFil: Ivanova, Alexandra D.. Lomonosov Moscow State University; RusiaFil: Kutaeva, Galina A.. Pitirim Sorokin Syktyvkar State University; RusiaFil: Pliss, Michael G.. Almazov National Medical Research Centre; RusiaFil: Lopatina, Ekaterina V.. University of Saint Petersburg; RusiaFil: Vaykshnorayte, Marina A.. Ural Branch Of Russian Academy Of Sciences; RusiaFil: Diez, Emiliano Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; ArgentinaFil: Azarov, Jan E.. Ural Branch of Russian Academy of Sciences; Rusia. Pitirim Sorokin Syktyvkar State University; Rusi

MODULATION OF THE TRANSDUCER FUNCTION OF Na+,K+-АТPase: NEW MECHANISM OF HEART REMODELING

Endogenous digitalis-like factors were found in the mammalian and human blood. It was the starting point for the elucidation of the new non-pumping function of the NaThe accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Clinical Case of Unilateral Laterothoracic Exanthem

Unilateral laterothoracic exanthem (ULE) is relatively rare self-limited pediatric skin inflammatory disease; its etiology still remains unknown. The diagnosis can be established due to specific clinical findings: presence of spotted and papular rash on one side of the body, more often in the axillary or inguinal area with possible generalization. This article presents the clinical case of ULE in the girl (4 years 8 months), it shows the dynamics of exanthematous syndrome at this pathology on different stages of the disease course

Development and Research of Technology Manufacturing of Semi-Finished Products from Solder Alloys Based on Silver

Proposed new compositions of solder alloys based on silver. Developed technological schemes of production of wire diameter 1,2 mm. Developed scheme of production of wire diameter 1,2 mm from alloy SAg-40I with operation combined casting, rolling and extruding. Modeling of temperature-rate parameters of the process of combined casting, rolling and extruding has been made. Designed and constructed the installation CCRE-80. The analysis of the structure and properties of semi-finished solder alloys based on silver has been done.Предложены новые составы припойных сплавов на основе серебра. Разработаны технологические схемы производства проволоки диаметром 1,2 мм. Разработана схема производства проволоки диаметром 1,2 мм из сплава ПСр-40И с применением операции совмещенного литья, прокатки и прессования. Проведено моделирование температурно- скоростных параметров процесса совмещенного литья, прокатки и прессования. Спроектирована и изготовлена установка СЛИПП-80. Проведен анализ структуры и свойств полуфабрикатов из припойных сплавов на основе серебра