John's gospel and the Johannine church: A mirror of events wifhin a text or/and a window on events within a church

John's gospel and the Johannine church: A mirror of events within a textor/and a window on events within a church.This paper investigates the problem of an alleged Johannine church/ school. The hermeneutical paradigms and results of two mainline exegetical methods are assessed, namely the historico-critical method and literary criticism (a textimmanent procedure). Their respective approaches of using the text of John's Gospel (JG) as a window and as a mirror are correlated. An analysis of the narrator's commentary (footnotes, asides) furnished important conclusions. They are that a referential correlation exists between the worlds within and outside the text. The direction of reference runs from the textextemal to the textintemal worlds, furthermore, thepragmatics of JG as a religio-historical text justifies the assumption that the readers/church within and outside the text are to be identified as ambivalent entities consisting of both Jewish and Hellenistic elements

Introgression of Brown Norway \u3cem\u3eCYP4A\u3c/em\u3e Genes onto the Dahl Salt-Sensitive Background Restores Vascular Function in SS-5\u3csup\u3eBN\u3c/sup\u3e Consomic Rats

The present study tested the hypothesis that the Dahl SS (salt-sensitive) rat has vascular dysfunction due, in part, to the up-regulation of the CYP4A/20-HETE (cytochrome P450 ω-hydroxylase 4A)/20-hydroxyeicosatetraenoic acid) system. To assess the role of vascular 20-HETE, SS rats were compared with SS-5BN consomic rats, carrying CYP4A alleles on chromosome 5 from the normotensive BN (Brown Norway) introgressed on to the SS genetic background. Cerebral arteries from SS-5BN rats had less CYP4A protein than arteries from SS rats fed either NS (normal-salt, 0.4% NaCl) or HS (high-salt, 4.0% NaCl) diet. ACh (acetylcholine)-induced dilation of MCAs (middle cerebral arteries) from SS and SS-5BN rats was present in SS-5BN rats fed on either an NS or HS diet, but absent in SS rats. In SS rats fed on either diet, ACh-induced dilation was restored by acute treatment with the CYP4A inhibitor DDMS (N-methyl-sulfonyl-12,12-dibromododec-11-enamide) or the 20-HETE antagonist 20-HEDE [20-hydroxyeicosa-6(Z),15(Z)-dienoic acid]. The restored response to ACh in DDMS-treated SS rats was inhibited by L-NAME (NGnitro-L-arginine methyl ester) and unaffected by indomethacin or MS-PPOH [N-methylsulfonyl-6-(2-propargyloxyphenyl)hexanamide]. Vascular relaxation responses to the NO donor C5FeN6Na2O were intact in both SS and SS-5BN rats and unaffected by the acute addition of DDMS, indicating that the vascular dysfunction of the SS rat is due to a reduced bioavailability of NO instead of failure of the VSMCs (vascular smooth muscle cells) to respond to the vasodilator. Superoxide levels in cerebral arteries of SS-5BN rats [evaluated semi-quantitatively by DHE (dihydroethidium) fluorescence] were lower than those in the arteries of SS rats. These findings indicate that SS rats have an up-regulation of the CYP4A/20-HETE pathway resulting in elevated ROS (reactive oxygen species) and reduced NO bioavailability causing vascular dysfunction

Gender-Specific Protection from Microvessel Rarefaction in Female Hypertensive Rats

Epidemiologic studies reveal that women have a significantly lower age-adjusted morbidity and mortality from cardiovascular disease than men, suggesting that gender is a cardiovascular disease risk factor. The mechanism of the “gender protection” is unknown. In this study, we investigated the microvascular remodeling in reduced renal mass plus a high salt (4.0% NaCl) diet model of hypertension (RRM + HS). We hypothesized that women would be protected from the increase in blood pressure and from the microvascular rarefaction associated with RRM + HS hypertension. Studies were designed to determine whether female rats were less susceptible to changes in microvessel density during RRM + HS. Microvessel density was measured in male and female low salt (0.4% LS) sham-operated controls (Sham + LS) and after 3 days or 4 weeks of RRM + HS hypertension. The microcirculation of hind limb (medial and lateral gastrocnemius, plantaris, soleus) muscles was visualized using rhodamine-labeled Griffonia simplicifolia I lectin. Tissue sections were examined by videomicroscopy and microvessel density was determined by quantitative stereology. As shown previously, mean arterial pressure increased to 160 ± 8 mm Hg and microvessel density decreased (\u3e30% decrease in all beds) in male RRM + HS. In contrast, mean arterial pressure of female RRM + HS rats was modestly increased from 101 ± 2 to 118 ± 4 mm Hg. Despite previous results showing a reduction in microvessel density of both normotensive and hypertensive male rats on a high salt diet, microvessel density of female RRM + HS rats was not reduced at either time. These results suggest that gender protection in the RRM rat extends beyond an attenuation of the increase in pressure to an immunity from microvascular rarefaction

Influence of localised smooth steps on the instability of a boundary layer

We consider a smooth, spanwise-uniform forward facing step de ned by the Gauss error function of height 4-30% and four times the width of the local boundary layer thickness δ_99. The boundary layer flow over a smooth forward-facing stepped plate is studied with particular emphasis on stabilisation and destabilisation of the two-dimensional Tollmien- Schlichting (TS) waves and subsequently on three-dimensional disturbances at transition. The interaction between TS waves at a range of frequencies and a base flow over a single or two forward facing smooth steps is conducted by linear analysis. The results indicate that for a TS wave with a frequency F 2 [140; 160] (F=! =U21 106 where ! and U1 denote the perturbation angle frequency and freestream velocity magnitude, respectively), the amplitude of the TS wave is attenuated in the unstable regime of the neutral stability curve corresponding to a at plate boundary layer. Furthermore, it is observed that two smooth forward facing steps lead to a more acute reduction of the amplitude of the TS wave. When the height of a step is increased to more than 20% of the local boundary layer thickness for a xed width parameter, the TS wave is amplified and thereby a destabilisation e ect is introduced. Therefore, stabilisation or destabilisation effect of a smooth step is typically dependent on its shape parameters. To validate the results of the linear stability analysis, where a TS wave is damped by the forward facing smooth steps direct numerical simulation (DNS) is performed. The results of the DNS correlate favorably with the linear analysis and show that for the investigated frequency of the TS wave, the K-type transition process is altered whereas the onset of the H-type transition is delayed. The results of the DNS suggest that for the perturbation with the non-dimensional frequency parameter F = 150 and in the absence of other external perturbations, two forward facing smooth steps of height 5% and 12% of the boundary layer thickness delayed H-type transition scenario and completely suppresses it for the Ktype transition. By considering Gaussian white noise with both xed and random phase shift, it is demonstrated by DNS that transition is postponed in time and space by two forward facing smooth steps

On the conditional distribution of the mean of the two closest among a set of three observations

Chemical analyses of raw materials are often repeated in duplicate or triplicate. The assay values obtained are then combined using a predetermined formula to obtain an estimate of the true value of the material of interest. When duplicate observations are obtained, their average typically serves as an estimate of the true value. On the other hand, the "best of three" method involves taking three measurements and using the average of the two closest ones as estimate of the true value. In this paper, we consider another method which potentially involves three measurements. Initially two measurements are obtained and if their difference is sufficiently small, their average is taken as estimate of the true value. However, if the difference is too large then a third independent measurement is obtained. The estimator is then defined as the average between the third observation and the one among the first two which is closest to it. Our focus in the paper is the conditional distribution of the estimate in cases where the initial difference is too large. We find that the conditional distributions are markedly different under the assumption of a normal distribution and a Laplace distribution.Comment: 12 pages, 6 figure

On the conditional distribution of the mean of the two closest among a set of three observations

Chemical analyses of raw materials are often repeated in duplicate or triplicate. The assay values obtained are then combined using a predetermined formula to obtain an estimate of the true value of the material of interest. When duplicate observations are obtained, their average typically serves as an estimate of the true value. On the other hand, the "best of three" method involves taking three measurements and using the average of the two closest ones as estimate of the true value. In this paper, we consider another method which potentially involves three measurements. Initially two measurements are obtained and if their difference is sufficiently small, their average is taken as estimate of the true value. However, if the difference is too large then a third independent measurement is obtained. The estimator is then defined as the average between the third observation and the one among the first two which is closest to it. Our focus in the paper is the conditional distribution of the estimate in cases where the initial difference is too large. We find that the conditional distributions are markedly different under the assumption of a normal distribution and a Laplace distribution

Analytical solution to the 1D nonlinear elastodynamics with general constitutive laws

International audienceUnder the hypothesis of small deformations, the equations of 1D elastodynamics write as a 2 × 2 hyperbolic system of conservation laws. Here, we study the Riemann problem for convex and nonconvex constitutive laws. In the convex case, the solution can include shock waves or rarefaction waves. In the nonconvex case, compound waves must also be considered. In both convex and nonconvex cases, a new existence criterion for the initial velocity jump is obtained. Also, admissibility regions are determined. Lastly, analytical solutions are completely detailed for various constitutive laws (hyperbola, tanh and polynomial), and reference test cases are proposed

Evaluation of Vascular Control Mechanisms Utilizing Video Microscopy of Isolated Resistance Arteries of Rats

This protocol describes the use of in vitro television microscopy to evaluate vascular function in isolated cerebral resistance arteries (and other vessels), and describes techniques for evaluating tissue perfusion using Laser Doppler Flowmetry (LDF) and microvessel density utilizing fluorescently labeled Griffonia simplicifolia (GS1) lectin. Current methods for studying isolated resistance arteries at transmural pressures encountered in vivo and in the absence of parenchymal cell influences provide a critical link between in vivo studies and information gained from molecular reductionist approaches that provide limited insight into integrative responses at the whole animal level. LDF and techniques to selectively identify arterioles and capillaries with fluorescently-labeled GS1 lectin provide practical solutions to enable investigators to extend the knowledge gained from studies of isolated resistance arteries. This paper describes the application of these techniques to gain fundamental knowledge of vascular physiology and pathology in the rat as a general experimental model, and in a variety of specialized genetically engineered designer rat strains that can provide important insight into the influence of specific genes on important vascular phenotypes. Utilizing these valuable experimental approaches in rat strains developed by selective breeding strategies and new technologies for producing gene knockout models in the rat, will expand the rigor of scientific premises developed in knockout mouse models and extend that knowledge to a more relevant animal model, with a well understood physiological background and suitability for physiological studies because of its larger size

The size of two-body weakly bound objects : short versus long range potentials

The variation of the size of two-body objects is investigated, as the separation energy approaches zero, with both long range potentials and short range potentials having a repulsive core. It is shown that long range potentials can also give rise to very extended systems. The asymptotic laws derived for states with angular momentum l=1,2 differ from the ones obtained with short range potentials. The sensitivity of the asymptotic laws on the shape and length of short range potentials defined by two and three parameters is studied. These ideas as well as the transition from the short to the long range regime for the l=0 case are illustrated using the Kratzer potential.Comment: 5 pages, 3 figures, submitted to Physical Review Letter