Effects of Land Management Strategies on the Dispersal Pattern of a Beneficial Arthropod

Several arthropods are known to be highly beneficial to agricultural production. Consequently it is of great relevance to study the importance of land management and land composition for the conservation of beneficial aphid-predator arthropod species in agricultural areas. Therefore our study focusing on the beneficial arthropod Bembidion lampros had two main purposes: I) identifying the physical barriers to the species’ dispersal in the agricultural landscape, and II) assessing the effect of different land management strategies (i.e. use of pesticides and intensiveness) on the dispersal patterns. The study was conducted using genetic analysis (microsatellite markers) applied to samples from two agricultural areas (in Denmark) with different agricultural intensity. Land management effects on dispersal patterns were investigated with particular focus on: physical barriers, use of pesticide and intensity of cultivation. The results showed that Bembidion lampros disperse preferably through hedges rather than fields, which act as physical barriers to gene flow. Moreover the results support the hypothesis that organic fields act as reservoirs for the re-colonization of conventional fields, but only when cultivation intensity is low. These results show the importance of non-cultivated areas and of low intensity organic managed areas within the agricultural landscape as corridors for dispersal (also for a species typically found within fields). Hence, the hypothesis that pesticide use cannot be used as the sole predictor of agriculture’s effect on wild species is supported as land structure and agricultural intensity can be just as important

Landscape population genetics and the role of organic farming

This project aims at understanding the effect of different farming systems on the genetic diversity of common agricultural species. It is well known that organic farming generally improves the biodiversity and abundance of species in the agricultural landscape (Hole et al., 2005). A reduction in species number and abundance has been shown as a result of the intensification of farming suggesting a relationship between farming intensity and species abundance (e.g. Stoate et al., 2001). Anyway, none of the studies that investigated the effects of pesticides presence and farming intensity has investigated the effect on the genetic diversity and isolation of the populations. It has been shown that, despite the theoretical expectations, also very abundant species like Abax parallelepipedus can be divided in isolated and genetically distinct populations within very few years in response to human activity (e.g. construction of streets: Keller et al., 2004). Therefore, we chose two common agricultural species (field vole, Microtus agrestis, and a non-pest ground beetle, Bembidion lampros) belonging to different taxa and with different dispersal abilities, to investigate the effect of pesticide use and intensiveness of farming on their genetic structuring and diversity

On the relaxation of a variational principle for the motion of a vortex sheet in perfect fluid

A certain two-phase model is considered, which stands some relation with a description of the vortex sheet configuration in a perfect fluid. Both a sharp and a relaxed version of the evolution can be understood as a geodesic equation. The instability of the model is then determined by means of a curvature tensor

Wildlife friendly agriculture: which factors do really matter? A genetic study on field vole

The distribution of genetic differentiation and the directions of gene flow were determined mainly by landscape factors: thus the expectation that organic fields act as genetic reservoir was not met. The fact that agricultural area presented more sub-populations than the undisturbed one, together with the importance of connectivity and habitat size in shaping gene flow and genetic differentiation, shows that switching to organic farming might not be enough to ensure the conservation of species in the agricultural environment. These results emphasise the need to include landscape structure in management policies

Chromosomal and Cytoplasmic Analysis of Heat Shock Resistance in Natural Populations of Drosophila Melanogaster

We evaluated genetic differences between two populations of Drosophila melanogaster that differed in thermal tolerance. Adults of one tropical population (Mali) survived heat shock (39.5°C for 30 min.) at 84%. By contrast, those from a strain collected in Denmark survived at a rate of only 53%. The greatest effect on variation was differences in cytoplasms, but variation in chromosomes 2 and 1 also played a role on tolerance. Heat shock proteins, however, reside on chromosome 3 and, therefore, variation at these sites is low or differences had little effect on results obtained from the methods employed

On the brink between extinction and persistence

The nature of size fluctuations is crucial in forecasting future population persistence, independently of whether the variability stems from external forces or from the dynamics of the population renewal process. The risk of intercepting zero is highly dependent on the way the variance of the population size relates to its mean. The minimum population size required for a population not to go extinct can be determined by a scaling equation relating the variance to the arithmetic mean. By the use of a derived expression for the harmonic mean defined by the parameters of the scaling equation we show how it is possible to separate the domains of persistence from those of extinction and to facilitate the identification of populations on the brink of extinction

A major QTL affects temperature sensitive adult lethality and inbreeding depression in life span in Drosophila melanogaster

<p>Abstract</p> <p>Background</p> <p>The study of inbreeding depression has major relevance for many disciplines, including conservation genetics and evolutionary biology. Still, the molecular genetic basis of this phenomenon remains poorly characterised, as knowledge on the mechanistic causes of inbreeding depression and the molecular properties of genes that give rise to or modulate its deleterious effects is lacking. These questions warrant the detailed study of genetic loci giving rise to inbreeding depression. However, the complex and polygenic nature of general inbreeding depression makes this a daunting task. Study of inbreeding effects in specific traits, such as age-specific mortality and life span, provide a good starting point, as a limited set of genes is expected to be involved.</p> <p>Results</p> <p>Here we report on a QTL mapping study on inbreeding related and temperature sensitive lethality in male <it>Drosophila melanogaster</it>. The inbreeding effect was expressed at moderately high temperature, and manifested itself as severe premature mortality in males, but not in females. We used a North Carolina crossing design 3 to estimate average dominance ratio and heritability. We found the genetic basis of the lethal effect to be relatively simple, being due mainly to a single recessive QTL on the left arm of chromosome 2. This locus colocalised with a QTL that conditioned variation in female life span, acting as an overdominant locus for this trait. Male life span was additionally affected by variation at the X-chromosome.</p> <p>Conclusion</p> <p>This demonstrates that analysis of large conditional lethal effects is a viable strategy for delineating genes which are sensitive to inbreeding depression.</p