Molecular analysis of the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli

Bibliography: pages 135-150.This dissertation represents a continuation of the research on the sucrose utilization system of the aerobic, collagenolytic, halotolerant, Gram-negative bacterium Vibrio alginolyticus. The V. alginolyticus sucrose utilization system originally cloned into Escherichia coli on plasmid pVS100 involves a sucrase enzyme (gene scrB), and a sucrose uptake system. Synthesis of the sucrase and sucrose uptake system in V. alginolyticus and E. coli(pVS100) is regulated. The nucleotide sequence and analysis of DNA regions encoding the sucrose uptake and regulatory functions are presented here. An investigation of the expression of the. V. alginolyticus sucrose utilization system in Bacillus subtilis is also presented

Towards a framework for cooperation:spatial public diplomacy on the island of Ireland

European spatial planning arguments advocate a blend of strategic thinking, coordination and related initiatives to promote and secure territorial cohesion. These ambitions embrace a set of normative agendas around economic, social and environmental convergence, competitiveness, policy coordination and efficient infrastructure provision across space. In practice, territorial management then involves devising interventions across inter-connecting scales of governance which comprise complex agency relations, differentiated places and defined communities. In transnational contexts, attempts to foster appropriate spatial governance arrangements and relations across sovereign borders necessitate re-crafting planning and development cultures and service delivery practices to advance territorial cohesion. Transnational working necessarily involves cooperation across an extended range of institutions, interests, influences and potential actors. This paper examines attempts to secure bi-lateral commitment to a joint planning framework for the two distinct territories on the island of Ireland. Specifically, it traces the formal and informal activities involved in the development of the 2013 Framework for Cooperation between the Republic of Ireland and Northern Ireland. Informed by ideas of cross-border regionalism, it discusses the necessary spatial public diplomacy involved in the social reconstruction of strategic spatial planning to improve policy coordination and cross-border working

Data Visualization and Analysis for Climate Studies using NASA Giovanni Online System

With many global earth observation systems and missions focused on climate systems and the associated large volumes of observational data available for exploring and explaining how climate is changing and why, there is an urgent need for climate services. Giovanni, the NASA GES DISC Interactive Online Visualization ANd ANalysis Infrastructure, is a simple to use yet powerful tool for analysing these data for research on global warming and climate change, as well as for applications to weather. air quality, agriculture, and water resources

Correlation of stress and predisposition in onset of illness in Masters of Social Work students

This study addressed the specific problem of whether there is a significant correlation between stress and the onset of predisposed disease. Because most graduate programs are stressful and it is known that several social work graduate students in one cohort at CSUSB were diagnosed with diabetes as well as migraines and depression, the population for this study was Master\u27s of Social Work (MSW) students at California State University, San Bernardino, (CSUSB)

Demonstration of non-Markovian process characterisation and control on a quantum processor

In the scale-up of quantum computers, the framework underpinning fault-tolerance generally relies on the strong assumption that environmental noise affecting qubit logic is uncorrelated (Markovian). However, as physical devices progress well into the complex multi-qubit regime, attention is turning to understanding the appearance and mitigation of correlated -- or non-Markovian -- noise, which poses a serious challenge to the progression of quantum technology. This error type has previously remained elusive to characterisation techniques. Here, we develop a framework for characterising non-Markovian dynamics in quantum systems and experimentally test it on multi-qubit superconducting quantum devices. Where noisy processes cannot be accounted for using standard Markovian techniques, our reconstruction predicts the behaviour of the devices with an infidelity of $10^{-3}$. Our results show this characterisation technique leads to superior quantum control and extension of coherence time by effective decoupling from the non-Markovian environment. This framework, validated by our results, is applicable to any controlled quantum device and offers a significant step towards optimal device operation and noise reduction

Creating a useful vascular center: a statewide survey of what primary care physicians really want

AbstractObjectiveMultidisciplinary vascular centers (VCs) have been proposed to integrate vascular patient care. No studies, however, have assessed referring physician interest or which services should be provided. A statewide survey of primary care physicians (PCPs) was performed to answer these questions.MethodsQuestionnaires were mailed to 3711 PCPs, asking about familiarity with vascular disease, potential VC usage, and services VCs should provide. Univariate and multivariate analysis was used to determine which PCPs would refer patients, the services desired, and which patients would be referred.ResultsOf 1006 PCPs who responded, 66% would refer patients to a VC, especially patients younger than 50 years (P < .001) and those with lower extremity disease (P < .001) or abdominal aortic aneurysm (P < .001). PCPs practicing within 50 miles of a VC (P < .001), those in practice less than 5 years (P < .001), and those without specific training in vascular disease during residency (P = .004) were most likely to refer patients. Vascular surgery (97%), interventional radiology (90%), and a noninvasive vascular laboratory (82%) were considered the most important services, and physician educational services (62%) were also desirable. PCPs did not think cardiology, cardiac surgery, smoking cessation programs, or diabetes or lipid management are needed. Reasons for VC nonuse included travel distance (23%), sufficient local services (21%), and insurance issues (12%). Only 16% of PCPs believe that their patients with vascular disease currently receive optimal care.ConclusionThere is considerable interest in VCs among PCPs. In contrast to recently described models, VCs need not incorporate cardiology, cardiac surgery, smoking cessation programs, or diabetes or lipid management. VCs should include vascular surgery, interventional radiology, a noninvasive vascular laboratory, and physician educational services

Tactics and Operational Research in the Battle of the Atlantic in WWII

This study concerns the naval campaign in the 1939-1945 conflict between the Allied and Axis forces known as the ‘Battle of the Atlantic’. To facilitate the understanding of this battle a computer-based battle model of a typical engagement between the opposing forces was created. The key naval assets in this conflict were the Allied merchant ships carrying vital supplies that were organised into convoys and were escorted for their protection by warships. On the Axis side were submarines (U-boats) including their organisation into Wolf Packs. The Axis goal for the U-boats was the cutting off of the supply of vital war materials, especially oil and fuel, that were carried in the Allied convoys to Britain and the Allied forces in the UK, Mediterranean theatre and on the Russian front. Allied air power played a key role eventually, as did radio signal detection and interception. The focus of the modelling work was on the battles over the convoys SC107 and TM1, which represented serious Allied failure and Axis tactical, but not strategic, victory. The model created was a convoy centric kinematic one and was not a dynamic model of forces and accelerations. During this study, it was found that the U-boat commanders were not using their U-boats and torpedoes to the full efficiency that they could have achieved. The reason lay in the direct orders to attack individually and not simultaneously. A counterfactual aspect was created in the model to explore the effects of simultaneous formation attacks by all the members of a Wolf Pack. A profound increase in sinking of convoy ships per U-boat was obtained and reductions in U-boat losses identified. If used this could have led to a temporary Axis victory in the Atlantic in 1942-1943, and might even have delayed Allied victory and impacted post war Europe