Применение уравнений Френеля в статистическом анализе эллипсометрических данных - определение оптических показателей анодно полученных плёнок из TiO2

Оптические показатели TI02 плёнок , определены эллилсометрически при стандартных ех-ситовых условиях в видимой спектральной области. Плёнки , изготовленные из Ti02, получены анодным окислением электрополирован:ной титановой поверхности в области потенциала от О до 100 V. Путём вычисления:, с помощью измеренных эллипсометрических параметров L1 и Р и Френеловых уравнений, показано , что численное значение оптических показателей уменьшае11ся: с изменением т олщины плёнки, и что у тол·стых плёнок они получают постоянные значения:. Исползован и ем различных спос обо в выч исления: пок.азано, каким образом можно получить наиболее точные рез у ль таты, т. е. самая: маленькая: погрешность при статистической обработке эллипсометрических д анных

Применение уравнений Френеля в статистическом анализе эллипсометрических данных - определение оптических показателей анодно полученных плёнок из TiO2

Оптические показатели TI02 плёнок , определены эллилсометрически при стандартных ех-ситовых условиях в видимой спектральной области. Плёнки , изготовленные из Ti02, получены анодным окислением электрополирован:ной титановой поверхности в области потенциала от О до 100 V. Путём вычисления:, с помощью измеренных эллипсометрических параметров L1 и Р и Френеловых уравнений, показано , что численное значение оптических показателей уменьшае11ся: с изменением т олщины плёнки, и что у тол·стых плёнок они получают постоянные значения:. Исползован и ем различных спос обо в выч исления: пок.азано, каким образом можно получить наиболее точные рез у ль таты, т. е. самая: маленькая: погрешность при статистической обработке эллипсометрических д анных

An Essential Role for Katanin p80 and Microtubule Severing in Male Gamete Production

Katanin is an evolutionarily conserved microtubule-severing complex implicated in multiple aspects of microtubule dynamics. Katanin consists of a p60 severing enzyme and a p80 regulatory subunit. The p80 subunit is thought to regulate complex targeting and severing activity, but its precise role remains elusive. In lower-order species, the katanin complex has been shown to modulate mitotic and female meiotic spindle dynamics and flagella development. The in vivo function of katanin p80 in mammals is unknown. Here we show that katanin p80 is essential for male fertility. Specifically, through an analysis of a mouse loss-of-function allele (the Taily line), we demonstrate that katanin p80, most likely in association with p60, has an essential role in male meiotic spindle assembly and dissolution and the removal of midbody microtubules and, thus, cytokinesis. Katanin p80 also controls the formation, function, and dissolution of a microtubule structure intimately involved in defining sperm head shaping and sperm tail formation, the manchette, and plays a role in the formation of axoneme microtubules. Perturbed katanin p80 function, as evidenced in the Taily mouse, results in male sterility characterized by decreased sperm production, sperm with abnormal head shape, and a virtual absence of progressive motility. Collectively these data demonstrate that katanin p80 serves an essential and evolutionarily conserved role in several aspects of male germ cell development

Ellipsometric in situin~situ study of the titanium surfaces during the anodization

The titanium surfaces immersed in 0.5 mol.dm$^{-3}$ H$_{2}$SO$_{4}$ and anodized in the voltage region from 0 to 100 V have been studied by in situ ellipsometric measurements. The thickness, inhomogeneity, and porosity of the formed films rise with increasing applied voltage. A computational method is presented for obtaining both the refractive indices and thickness of an unknown film on a reflecting substrate of know optical constants. The complex index of refraction and thickness of generated films were calculated comparing the theoretical computed curve with experimental loci $\Delta$ and $\Psi$ obtained from in situ ellipsometric measurements

An ESR, Mass Spectrometry and Fluorescence microscopy approach to study the stearic acid derivatives anchoring in cells

peer reviewedLateral phase separations in biological membranes are of great interest, making Electron Spin Resonance (ESR) spectroscopy combined with spin labelling a non destructive and sensitive technique for the study of lipid rafts. This is currently accepted that spin probe localization is on the plasma membrane. However, no study confirms this hypothesis. Herein, we report, for the first time, an accurate multi spectral method for the quantification of lipid spin label presence in every sub-cellular fraction. Cells were incubated with 5-doxyl stearic acid derivative and then sub-fractionated. Results of our multimodal spectroscopy approach ubiquitously demonstrate that the presence of ESR spin label only sets in the plasma membranes

The hidden genetics of epilepsy—a clinically important new paradigm

Understanding the aetiology of epilepsy is essential both for clinical management of patients and for conducting neurobiological research that will direct future therapies. The aetiology of epilepsy was formerly regarded as unknown in about three-quarters of patients; however, massively parallel gene-sequencing studies, conducted in a framework of international collaboration, have yielded a bounty of discoveries that highlight the importance of gene mutations in the aetiology of epilepsy. These data, coupled with clinical genetic studies, suggest a new paradigm for use in the clinic: many forms of epilepsy are likely to have a genetic basis. Enquiry about a genetic cause of epilepsy is readily overlooked in the clinic for a number of understandable but remediable reasons, not least an incomplete understanding of its genetic architecture. In addition, the importance of de novo mutagenesis is often underappreciated, particularly in the epileptic encephalopathies. Other genomic surprises are worth emphasizing, such as the emerging evidence of a genetic contribution to focal epilepsies—long regarded as acquired conditions—and the complex role of copy number variation. The importance of improved understanding of the genetics of the epilepsies is confirmed by the positive outcomes, in terms of treatment selection and counselling, of receiving a genetic diagnosi