Zbyszko Chojnicki. Jak można być strażnikiem ładu intelektualnego w epoce kryzysu nauki?

Celem artykułu jest prezentacja roli prof. Zbyszka Chojnickiego w kształtowaniu programu metodologicznego polskiej geografii społeczno-ekonomicznej i próba oceny ewolucji jego autorytetu naukowego w okresie paradygmatycznym i postparadygmatycznym rozwoju dyscypliny

Disposition kinetics of robenacoxib following intravenous and oral administration in geese (Anser anser domesticus)

: Robenacoxib (RX) is a veterinary cyclooxygenase-2 selective inhibitor drug. It has never been tested on birds and is only labelled for use in cats and dogs. The purpose of this study was to assess its pharmacokinetics in geese after single intravenous (IV) and oral (PO) administrations. Four-month healthy female geese (n = 8) were used. Geese were subjected to a two-phase, single-dose (2 mg/kg IV, 4 mg/kg PO), open, longitudinal study design with a four-month washout period between the IV and the PO phases. Blood was collected from the left wing vein to heparinized tubes at 0, 0.085 (for IV only), 0.25, 0.5, 0.75, 1, 1.5, 2, 4, 6, 8, 10, and 24 h. Plasma RX concentrations were measured using HPLC coupled to an UV detector, and the data were pharmacokinetically analysed using ThothProTM 4.3 software in a non-compartmental approach. Following IV administration, terminal elimination half-life, volume of distribution, and total clearance were 0.35 h, 0.34 L/kg, and 0.68 L/h/kg, respectively. For the PO route, the mean peak plasma concentration was 6.78 μg/mL at 0.50 h. The t1/2λz was very short and significantly different between the IV and PO administrations (0.35 h IV vs. 0.99 h PO), suggesting the occurrence of a flip-flop phenomenon. The Cl values corrected for the F% were significantly different between IV and PO administrations. It might have been a consequence of the longitudinal study design and the altered physiological and environmental conditions after a 4-month washout period. The absolute oral F% computed with the AUC method surpassed 150%, but after normalizing it to t1/2λz, it was 46%. In conclusion, the administration of RX might not be suitable for geese, due to its short t1/2λz

Robenacoxib pharmacokinetics in sheep following oral, subcutaneous, and intravenous administration

The aim of this study was to evaluate the pharmacokinetics (PK) of robenacoxib (RX), a COX-2 selective non-steroidal anti-inflammatory drug, in sheep after single subcutaneous (SC), oral (PO), and intravenous (IV) administration. Five healthy female sheep underwent a three-phase parallel study design with a washout period of 4 weeks, in which sheep received a 4 mg/kg SC dose in phase 1, a 4 mg/kg PO administration in phase 2, and a 2 mg/kg IV administration in phase 3. Plasma RX concentrations were measured over a 48 h period for each treatment using HPLC coupled to a UV multiple wavelength detector, and the PK parameters were estimated using a non-compartmental method. Following IV administration, terminal elimination half-life, volume of distribution at steady state, and total clearance were 2.64 h, 0.077 L/kg, and 0.056 L/h kg, respectively. The mean peak plasma concentrations following SC and PO administrations were 7.04 and 3.01 mu g/mL, respectively. The mean bioavailability following SC and PO administrations were 45.98% and 16.58%, respectively. The SC route may be proposed for use in sheep. However, the multi-dose and pharmacodynamic studies are necessary to establish more accurately its safety and efficacy in sheep

THE LONG-TERM IMPACT OF AMELIORATING DOSES OF HARD COAL FLY ASH ON SHAPING THE CONTENT OF SELECTED MICROELEMENTS IN AGRICULTURAL SOIL

The effect of ameliorating doses of hard coal fly ash (HCFA) on chemical properties of soil and on yields and chemical composition of crops was examined based on an experiment established in 1984. At first, typical agricultural crops were grown in the experiment, but in 1992 the field was turned into permanent grassland. The current study took place twenty-nine years after hard coal fly ash had been applied. Soil samples were collected from the 0–20 cm soil layer, corresponding to the compared treatments. The residual effect of HCFA doses increasing from 100 to 800 Mg·ha-1 caused a regular increase in the total content of Cd, Cr, Cu, Mn, Fe, Zn and Pb, as well as the forms of Zn, Cu, Mn, Fe and B soluble in 0.1 M HCl. The total content of the analyzed microelements increased by a maximum of 30% for cadmium up to 176% for zinc. The highest increase in the content of soluble forms ranged from 25% for Fe to 760% for boron. The share of Znbd, i.e. permanently bound to soil, to Zntot, i.e. the total zinc form, was positively correlated with pH in KCl, the C:N ratio and the content of Cu permanently bound with soil. Regarding the share of Febd in Fetot, correlations with pH in H2O and in KCl, content of organic matter (SOM) and the C:N ratio were demonstrated. No such correlations were proven with respect to the other elements. W doświadczeniu założonym w 1984 roku badano działanie melioracyjnych dawek popiołów ze spalania węgla kamiennego (hard coal fly ashes – HCFA) na właściwości fizykochemiczne gleby oraz na plonowanie i skład chemiczny roślin uprawnych. W pierwszych latach badań uprawiano typowe rośliny rolnicze, a w 1992 zmieniono charakter uprawy na trwały użytek zielony. Obecne badania wykonano po dwudziestu dziewięciu latach od zastosowania HCFA. Próbki gleby pobrano z poziomu 0–20 cm w nawiązaniu do porównywanych obiektów. Następcze działanie rosnących dawek HCFA od 100 do 800 Mg·ha-1 powodowało zarówno wyraźnie regularny wzrost zawartości całkowitej – Cd, Cr, Cu, Mn, Fe, Zn i Pb, jak i form rozpuszczalnych w 0.1 M HCl – Zn, Cu, Mn, Fe i B. Zawartość całkowita rozpatrywanych mikroskładników maksymalnie wzrosła o 30% w przypadku kadmu do 176 % w odniesieniu do cynku. Maksymalny wzrost zawartości form rozpuszczalnych kształtował się od 25 % w odniesieniu do żelaza do 760 % w przypadku boru. Udział Znbd tj. trwale związanego z glebą w stosunku do jego formy ogólnej Zntot, istotnie dodatnio korelował z pH w KCl i stosunek C:N oraz z Cu trwale związaną z glebą. W odniesieniu do udziału Febd w Fetot wykazano korelacje z pH w H2O, pH w KCl, SOM i C:N ratio. W odniesieniu do pozostałych badanych pierwiastków takich zależności nie wykazano

Doxycycline pharmacokinetics in geese

Funding Information: This work was supported by University of Pisa (ex 60%). The authors acknowledge ThothPro (Gdansk, Poland) for supplying the software used for the pharmacokinetic analysis. The authors are grateful to Mr. Zbigniew Kołodziej (Majątek Rutka, Puchaczow, Poland) for assistance conducting animal experiment and for supplying animals and facilities. The authors sincerely thank Dr. Victoria Llewelyn (Flinders University, Australia) for the scientific and English editing of the manuscript. Funding Information: This work was supported by University of Pisa (ex 60%). The authors acknowledge ThothPro (Gdansk, Poland) for supplying the software used for the pharmacokinetic analysis. The authors are grateful to Mr. Zbigniew Ko?odziej (Maj?tek Rutka, Puchaczow, Poland) for assistance conducting animal experiment and for supplying animals and facilities. The authors sincerely thank Dr. Victoria Llewelyn (Flinders University, Australia) for the scientific and English editing of the manuscript. Publisher Copyright: © 2021 The Authors. Journal of Veterinary Pharmacology and Therapeutics published by John Wiley &Sons Ltd.The study aims to describe the pharmacokinetics of doxycycline after a single intravenous and oral dose (20 mg/kg) in geese. In addition, two multiple-dose simulations have been performed to investigate the predicted plasma concentration after either a 10 or 20 mg/kg daily administration repeated consecutively for 5 days. Ten geese were enrolled in a two-phase cross-over study with a washout period of two weeks. All animals were treated intravenously and orally with doxycycline, and blood samples were collected up to 48 h after drug administration. Sample analysis was performed using a validated HPLC-UV method. A non-compartmental approach was used to evaluate the pharmacokinetic parameters of the drug. A long elimination half-life was observed (13 h). The area under the curve was statistically different between the two treatments, with the oral bioavailability being moderate (43%). The pharmacokinetic/pharmacodynamic index (%T>MIC) during the 48 h treatment period in the present study (71%) suggests that doxycycline appears to have therapeutic efficacy against some Mycoplasma species in the goose. The multiple-dose simulations showed a low accumulation index. A dosage of 10 mg/kg/day for 5 days seemed to be adequate for a good therapeutic efficacy without reaching unnecessarily high plasma concentrations.publishersversionPeer reviewe

Pharmacokinetics of thalidomide in dogs: can feeding affect it? A preliminary study

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Effect of feeding on the pharmacokinetics of vilazodone in dogs

Vilazodone (VLZ) is a drug approved for the treatment of major depressive disorder in humans but no data are available for dogs. The present study aimed to evaluate the pharmacokinetics of a single oral 40 mg dose of VLZ in healthy Labrador dogs (n = 6) in fasted and fed conditions. Dogs were randomly divided in two (n = 3) groups in a cross-over study design (2 x 2). Group I was administered with VLZ at 40 mg/dog after fasting over-night. Group II was fed prior to and after administration of the same dose. A two-week wash-out period was observed. Plasma samples collected underwent LC-MS/MS analysis. VLZ concentrations were quantified in dogs' plasma in two different windows of time: 30 min to 10 h for the fasted group and 4 h to 35 h for the fed group. The values for t(1/2 lambda z) were statistically different between the groups (fed, 4.6 +/- 1.1 h vs fasted, 1.7 +/- 0.2 h). Tmax drastically changed between the groups (fed, 10 h vs fasted, 1.5 h), while C-max did not significantly vary (fed, 39.4 +/- 5.6 ng/mL vs fasted, 38.7 +/- 4.8 ng/mL). The AUC value was always statistically higher in the fed group. As a result, the average relative oral fasted bioavailability of VLZ was low, 28.8 +/- 6.1%. In conclusion, feeding can affect the pharmacokinetics of VLZ in the dog

Pharmacokinetics and antibacterial activity of tiamulin after single and multiple oral administrations in geese

Tiamulin is an antibiotic approved exclusively in veterinary medicine, active against G-positive bacteria as well as Mycoplasma spp. and Leptospirae spp. The study was aimed to establish its pharmacokinetics and to evaluate drug effects on resistance in cloacal flora in vivo in geese. Eight healthy geese underwent to a two-phase longitudinal study (60 mg/kg single oral administration vs 60 mg/kg/day for 4 days) with a two-week wash-out period. Blood samples and cloacal swabs were collected at pre-assigned times. Minimal inhibitory concentration (MIC) has been evaluated for each isolated bacterial species. The pharmacokinetic parameters that significantly differed between the groups were Cmax (p = 0.024), AUC0-t (p = 0.031), AUC0-inf (p = 0.038), t1/2kel (p = 0.021), Cl/F (p = 0.036), and Vd/F (p = 0.012). Tiamulin exhibited a slow to moderate terminal half-life (3.13 h single; 2.62 h multiple) and a rapid absorption (1 h single; 0.5 h multiple) in geese, with an accumulation ratio of 1.8 after multiple doses. An in-silico simulation of multiple dosing did not reflect the results of the in vivo multiple dosage study. In both treatments, the MIC values were very high demonstrating a resistance (> 64 μg/ml) against tiamulin that can be present prior the drug administration for some strains, or emerge shortly after the commencing of treatment for some others

Intravascular embolization of the uterine arteries in the treatment of early postpartum haemorrhage – case report

Summary The article presents a case report of a patient with postpartum haemorrhage in the course of uterine atony with hypovolemic shock as a complication treated by uterine arteries embolization. In obstetrics and gynecology this procedure was first described in 1979 by Brown et al. Embolization is a rapid, secure, efficient and low-invasive alternative to surgical intervention when pharmacological treatment of postpartum haemorrhage fails