Ružička days : International conference 16th Ružička Days “Today Science – Tomorrow Industry” : Proceedings

Proceedings contains articles presented at Conference divided into sections: open lecture (1), chemical analysis and synthesis (3), chemical and biochemical engineering (8), food technology and biotechnology (8), medical chemistry and pharmacy (3), environmental protection (11) and meeting of young chemists (2)

Identification of primary aroma of white grape varietes must

Određivanje sortnih spojeva vina jedan je od najvažnijih koraka u procjeni arome i kvalitete vina. Glavni predstavnici primarne arome koji utječu na karakter i senzorske karakteristike vina su terpeni, C13-norizoprenoidi i C6-alkoholi. Uzorci mošta bijelih sorti grožđa analizirani su metodom plinske kromatografije s masenom spektrometrijom uz mikroekstrakciju na čvrstoj fazi (SPME-GC/MS). Identificirano je 15 različitih spojeva, a sortnoj aromi analiziranih uzoraka najviše doprinose eukaliptol (mirisa eukaliptusa), geranil aceton (slatka aroma), β-ionon (mirisa meda, ruže, ljiljana) te β-damaskenon (slatkog i bademastog mirisa).Determination of varietal compounds of wine is one of the most important step in the evaluation of aroma and wine quality. Main compounds of primary aroma that affect the character and sensory guality of wines are terpenes, C13-norisoprenoids and C6-alcohols. Must samples of white grape varietes were analyzed using solid-phase microextraction method followed by gas chromatography-mass spectrometry (SPME-GC/MS). 15 diferent compounds were determined, among them eucalyptol (eucalyptol aroma), geranyl acetone (sweet aroma), β-ionone (honey, spice, rose, lilac aroma) and β-damascenone (almond, sweet aroma) contribute to the varietal aroma of analyzed samples

Identification of primary aroma of white grape varietes must

Određivanje sortnih spojeva vina jedan je od najvažnijih koraka u procjeni arome i kvalitete vina. Glavni predstavnici primarne arome koji utječu na karakter i senzorske karakteristike vina su terpeni, C13-norizoprenoidi i C6-alkoholi. Uzorci mošta bijelih sorti grožđa analizirani su metodom plinske kromatografije s masenom spektrometrijom uz mikroekstrakciju na čvrstoj fazi (SPME-GC/MS). Identificirano je 15 različitih spojeva, a sortnoj aromi analiziranih uzoraka najviše doprinose eukaliptol (mirisa eukaliptusa), geranil aceton (slatka aroma), β-ionon (mirisa meda, ruže, ljiljana) te β-damaskenon (slatkog i bademastog mirisa).Determination of varietal compounds of wine is one of the most important step in the evaluation of aroma and wine quality. Main compounds of primary aroma that affect the character and sensory guality of wines are terpenes, C13-norisoprenoids and C6-alcohols. Must samples of white grape varietes were analyzed using solid-phase microextraction method followed by gas chromatography-mass spectrometry (SPME-GC/MS). 15 diferent compounds were determined, among them eucalyptol (eucalyptol aroma), geranyl acetone (sweet aroma), β-ionone (honey, spice, rose, lilac aroma) and β-damascenone (almond, sweet aroma) contribute to the varietal aroma of analyzed samples

Bioactive composition and biological effect of mint, rosemary and ginger extracts on the larynx and lung cancer cells

Biljne se vrste, posebno menta, ružmarin i đumbir zbog kemijskog sastava prisutnih spojeva i njihovog velikog antioksidacijskog potencijala već dugi niz godina tradicionalno koriste u liječenju različitih bolesti. Ovim je radom provedena detaljna karakterizacija bioaktivnih spojeva (polifenola, pigmenata, polisaharida) navedenih biljnih vrsta i njihovih različito pripremljenih ekstrakata. Također, ispitivani su i biološki učinci na stanice raka grkljana HEp2 i raka pluća A549 stanice. Najveći su udjeli polifenolnih i pigmentnih spojeva određeni u uzorcima mente, koji su pokazali i najveći antioksidacijski kapacitet. Đumbir je bio značajno siromašan ovim spojevima, uz iznimku najvećeg udjela polisaharida. Ekstrakcija biljnih sirovina vodom temperature 80 °C tijekom 15 minuta rezultirala je većim udjelom polifenolnih spojeva u ekstraktima u odnosu na istu provedenu vodom temperature 100 °C tijekom 30 minuta. Ekstrakti mente pokazali su citotoksičnost na obje stanične linije te prooksidativno djelovanje i indukciju lipidne peroksidacije na stanicama A549. Ekstrakti ružmarina djeluju prooksidativno i citotoksično na obje stanične linije te induciraju lipidnu peroksidaciju u stanicama A549. Ekstrakti đumbira štite stanice HEp2 i A549 od slobodnih radikala i oštećenja lipida.Plants, especially mint, rosemary and ginger are traditionally used for disease treatment because of their chemical composition and antioxidant capacity. In this thesis, detailed characterization of bioactive compounds (polyphenols, pigments, polysaccharides) in selected plant materials and their different extracts was investigated. Biological effects of these extracts on the larynx cancer cells HEp2 and lung cancer cells A549 were also evaluated. Mint sample had the highest amounts of polyphenols and pigments so the highest antioxidant capacity. Despite lowest amounts of the polyphenols and pigments, ginger sample had the highest level of polysaccharides. Extraction of plant raw materials with water at 80 °C for 15 minutes resulted in a greater share of polyphenolic compounds extracts when compared to the extraction at a temperature of 100 °C for 30 minutes. Cytotoxic effect was seen on the HEp2 cells and prooxidative effect with induction of the lipid peroxidation was observed in the A549 cells incubated with mint extract. Rosemary extract showed cytotoxic and prooxidative effect on the HEp2 and A549, and induction of the lipid peroxidation on the A549. Ginger extract decreased the concentration of free radicals and also protect lipids of HEp2 and A549 cells

Bioactive composition and biological effect of mint, rosemary and ginger extracts on the larynx and lung cancer cells

Biljne se vrste, posebno menta, ružmarin i đumbir zbog kemijskog sastava prisutnih spojeva i njihovog velikog antioksidacijskog potencijala već dugi niz godina tradicionalno koriste u liječenju različitih bolesti. Ovim je radom provedena detaljna karakterizacija bioaktivnih spojeva (polifenola, pigmenata, polisaharida) navedenih biljnih vrsta i njihovih različito pripremljenih ekstrakata. Također, ispitivani su i biološki učinci na stanice raka grkljana HEp2 i raka pluća A549 stanice. Najveći su udjeli polifenolnih i pigmentnih spojeva određeni u uzorcima mente, koji su pokazali i najveći antioksidacijski kapacitet. Đumbir je bio značajno siromašan ovim spojevima, uz iznimku najvećeg udjela polisaharida. Ekstrakcija biljnih sirovina vodom temperature 80 °C tijekom 15 minuta rezultirala je većim udjelom polifenolnih spojeva u ekstraktima u odnosu na istu provedenu vodom temperature 100 °C tijekom 30 minuta. Ekstrakti mente pokazali su citotoksičnost na obje stanične linije te prooksidativno djelovanje i indukciju lipidne peroksidacije na stanicama A549. Ekstrakti ružmarina djeluju prooksidativno i citotoksično na obje stanične linije te induciraju lipidnu peroksidaciju u stanicama A549. Ekstrakti đumbira štite stanice HEp2 i A549 od slobodnih radikala i oštećenja lipida.Plants, especially mint, rosemary and ginger are traditionally used for disease treatment because of their chemical composition and antioxidant capacity. In this thesis, detailed characterization of bioactive compounds (polyphenols, pigments, polysaccharides) in selected plant materials and their different extracts was investigated. Biological effects of these extracts on the larynx cancer cells HEp2 and lung cancer cells A549 were also evaluated. Mint sample had the highest amounts of polyphenols and pigments so the highest antioxidant capacity. Despite lowest amounts of the polyphenols and pigments, ginger sample had the highest level of polysaccharides. Extraction of plant raw materials with water at 80 °C for 15 minutes resulted in a greater share of polyphenolic compounds extracts when compared to the extraction at a temperature of 100 °C for 30 minutes. Cytotoxic effect was seen on the HEp2 cells and prooxidative effect with induction of the lipid peroxidation was observed in the A549 cells incubated with mint extract. Rosemary extract showed cytotoxic and prooxidative effect on the HEp2 and A549, and induction of the lipid peroxidation on the A549. Ginger extract decreased the concentration of free radicals and also protect lipids of HEp2 and A549 cells