Importance of clinical integration, morphological findings and immunophenotyping by flow cytometry in hairy cell leukemia diagnosis : three cases report

ANTECEDENTES: La leucemia de células peludas es una neoplasia linfoproliferativa de linfocitos B maduros, que puede infiltrar la médula ósea o el bazo. Representa el 2% de las leucemias linfoides y los hallazgos iniciales de laboratorio clínico incluyen desde citopenias hasta pancitopenia. El análisis morfológico y el patrón típico de infiltración de la médula ósea y el perfil inmunofenotípico incluye la expresión de CD11c, CD103, y CD25. CASO CLÍNICO: Se exponen tres casos de pacientes diagnosticados con leucemia de células peludas, con infecciones asociadas y larga evolución de la enfermedad, a quienes se prescribió cladribina y rituximab, con reacción satisfactoria. El diagnóstico incluyó la integración de análisis clínicos, morfo-patológicos, inmunofenotípicos y moleculares. CONCLUSIONES: La identificación de factores de riesgo y el diseño del estudio médico dependen de la notificación y vigilancia de pacientes con leucemia de células peludas, además de la inclusión de pruebas moleculares que permitan el seguimiento específico y la obtención de datos pronósticos y blancos terapéuticos para la mutación de BRAF.BACKGROUND: Hairy cell leukemia is a lymphoproliferative neoplasm of mature B cells, which can infiltrate the bone marrow or spleen. It represents 2% of lymphoid leukemias and initial clinical laboratory results range from cytopenias to pancytopenia. Morphological analysis and typical pattern of bone marrow infiltration and immunophenotypic profile includes expression of CD11c, CD103, and CD25. CLINICAL CASE: Three cases of patients diagnosed with hairy cell leukemia, with associated infections and long evolution of the disease, who were prescribed cladribine and rituximab, with satisfactory reaction, are presented. The complete diagnosis included: clinical, morpho-pathological, immuno-phenotypic and molecular analyses. CONCLUSIONS: The identification of risk factors and the design of the medical study depend on the notification and surveillance of patients with hairy cell leukemia, in addition to the inclusion of molecular tests that allow specific monitoring and the obtaining of prognostic data and therapeutic targets for the BRAF mutation.https://orcid.org/0000-0002-6563-7067https://orcid.org/0000-0002-3548-9016https://orcid.org/0000-0003-4999-8193https://orcid.org/0000-0002-8405-9311https://orcid.org/0000-0002-7147-425XRevista Internacional - No indexadaN

Endemic and epidemic human alphavirus infections in eastern Panama: An analysis of population-based cross-sectional surveys

Madariaga virus (MADV) has recently been associated with severe human disease in Panama, where the closely related Venezuelan equine encephalitis virus (VEEV) also circulates. In June 2017, a fatal MADV infection was confirmed in a community of Darien Province. We conducted a cross-sectional outbreak investigation with human and mosquito collections in July 2017, where sera were tested for alphavirus antibodies and viral RNA. In addition, by applying a catalytic, force-of-infection (FOI) statistical model to two serosurveys from Darien Province in 2012 and 2017, we investigated whether endemic or epidemic alphavirus transmission occurred historically. In 2017, MADV and VEEV IgM seroprevalences were 1.6% and 4.4%, respectively; IgG antibody prevalences were MADV: 13.2%, VEEV: 16.8%, Una virus (UNAV): 16.0%, and Mayaro virus: 1.1%. Active viral circulation was not detected. Evidence of MADV and UNAV infection was found near households, raising questions about its vectors and enzootic transmission cycles. Insomnia was associated withMADVand VEEV infections, depression symptoms were associated with MADV, and dizziness with VEEV and UNAV. Force-of-infection analyses suggest endemic alphavirus transmission historically, with recent increased human exposure to MADV and VEEV in Aruza and Mercadeo, respectively. The lack of additional neurological cases suggests that severe MADV and VEEV infections occur only rarely. Our results indicate that over the past five decades, alphavirus infections have occurred at low levels in eastern Panama, but that MADV and VEEV infections have recently increased-potentially during the past decade. Endemic infections and outbreaks of MADV and VEEV appear to differ spatially in some locations of eastern Panama.National Institute for Health ResearchRevisión por pare

Case Studies of Realtime Multiprocessors in Robotics

NRC publication: Ye

A Multi-Agent Architecture Integrating Learning and Fuzzy Techniques for Landmark-Based Robot Navigation

La publicación original está disponible en www.springerlink.com.This paper extends a navigation system implemented as a multi-agent system (MAS). The arbitration mechanism controlling the interactions between the agents was based on manually-tuned bidding functions. A difficulty with hand-tuning is that it is hard to handle situations involving complex tradeoffs. In this paper we explore the suitability of reinforcement learning for automatically tuning agents within a MAS to optimize a complex tradeoff, namely the camera use.Fullbright Joint Research Project and Plan Nacional Project DPI 2000-1352-C02-02. Dídac Busquets holds the CIRIT doctoral scholarship 2000FI-00191.Peer reviewe