Art On the Plate: Effect of Balance and Color On Attractiveness Of, Willingness to Try and Liking for Food

The study investigated whether balance and complexity (increased by the addition of color) in the presentation of food on a plate affect the attractiveness of the presentation much as those factors affect the attractiveness of works of art. In addition, the willingness to try the food and liking for the food in four presentations (monochrome-balanced, colored-balanced, monochrome-unbalanced, and colored-unbalanced) combining these two variables was measured. While color increased the attractiveness of the balanced presentation it did not increase the attractiveness of the unbalanced one. Subjects were more willing to try the monochrome than colored presentations. There was no effect of color or balance on liking for the flavor of the food. So, while manipulating color and balance in a food presentation affects its attractiveness, it does not alter how much one likes the flavor of the food

Alterations of Lipid Profile in Livers with Impaired Lipophagy

Non-alcoholic fatty liver disease (NAFLD) is characterized by excessive lipid accumulation in the liver. Various mechanisms such as an increased uptake in fatty acids or de novo synthesis contribute to the development of steatosis and progression to more severe stages. Furthermore, it has been shown that impaired lipophagy, the degradation of lipids by autophagic processes, contributes to NAFLD. Through an unbiased lipidome analysis of mouse livers in a genetic model of impaired lipophagy, we aimed to determine the resulting alterations in the lipidome. Observed changes overlap with those of the human disease. Overall, the entire lipid content and in particular the triacylglycerol concentration increased under conditions of impaired lipophagy. In addition, we detected a reduction in long-chain polyunsaturated fatty acids (PUFAs) and an increased ratio of n-6 PUFAs to n-3 PUFAs, which was due to the depletion of n-3 PUFAs. Although the abundance of major phospholipid classes was reduced, the ratio of phosphatidylcholines to phosphatidylethanolamines was not affected. In conclusion, this study demonstrates that impaired lipophagy contributes to the pathology of NAFLD and is associated with an altered lipid profile. However, the lipid pattern does not appear to be specific for lipophagic alterations, as it resembles mainly that described in relation to fatty liver disease

Polymorphisms in miRNA binding sites involved in metabolic diseases in mice and humans

Type 2 diabetes and obesity are well-studied metabolic diseases, which are based on genetic and epigenetic alterations in combination with an obesogenic lifestyle. The aim of this study was to test whether SNPs in miRNA-mRNA binding sites that potentially disrupt binding, elevate the expression of miRNA targets, which participate in the development of metabolic diseases. A computational approach was developed that integrates transcriptomics, linkage analysis, miRNA-target prediction data, and sequence information of a mouse model of obesity and diabetes. A statistical analysis demonstrated a significant enrichment of 566 genes for a location in obesity- and diabetes-related QTL. They are expressed at higher levels in metabolically relevant tissues presumably due to altered miRNA-mRNA binding sites. Of these, 51 genes harbor conserved and impaired miRNA-mRNA-interactions in human. Among these, 38 genes have been associated to metabolic diseases according to the phenotypes of corresponding knockout mice or other results described in the literature. The remaining 13 genes (e.g. Jrk, Megf9, Slfn8 and Tmem132e) could be interesting candidates and will be investigated in the future

Neatness Counts. How Plating Affects Liking for the Taste of Food

Two studies investigated the effect that the arrangement of food on a plate has on liking for the flavor of the food. Food presented in a neatly arranged presentation is liked more than the same food presented in a messy manner. A third study found that subjects expected to like the food in the neat presentations more than in the messy ones and would be willing to pay more for them. They also indicated that the food in the neat presentations came from a higher quality restaurant and that more care was taken with its preparation than the food in the messy presentations. Only the animal-based food was judged as being more contaminated when presented in a messy rather than a neat way. Neatness of the food presentation increases liking for the taste of the food by suggesting greater care on the part of the preparer. Two mechanisms by which greater care might increase liking are discussed

To RAP or Not to RAP: A Retrospective Comparison of the Effects of Retrograde Autologous Priming

Retrograde autologous priming (RAP) is a process used to reduce hemodilution associated with the initiation of cardiopulmonary bypass (CPB). Previous studies have reported potential benefits to RAP; however, many of these studies do not evaluate the benefits of RAP with limited preoperative fluid administration combined with a condensed CPB circuit. We examined clinical metrics of patients who underwent RAP versus those who did not undergo RAP prior to the initiation of CPB. This was a retrospective data review of 1,303 patients who underwent CPB in the setting of open-heart surgery for a 2-year period. RAP was used on all patients between June 1, 2017 and June 30, 2018 (n = 519) and not used on patients between July 1, 2018 and June 30, 2019 (n = 784). Both groups were subjected to a low-prime CPB circuit volume of 800–900 mL. We compared the clinical metrics for packed red blood cell (PRBC) transfusion, oxygen delivery, postoperative acute kidney injury (AKI), Albumin utilization, ventilator time, Intensive Care Unit length of stay (ICU LOS), and 30-day mortality between the two groups. Our data analysis showed there were no statistically significantly differences between the two groups on the incidence of postoperative AKI, PRBC administration, ventilator time, ICU LOS or 30-day mortality. In the RAP group, there was a statistically significant lower oxygen delivery and a statistically significant increased volume of Albumin administered postoperatively, although those differences were so small, they were potentially not clinically significant. Our analysis revealed no significant benefit to performing RAP with limited preoperative fluid administration and minimized CPB circuit prime volume. We formalized a process that included limiting preoperative fluid administration and minimizing the CPB circuit volume so that we were not required to RAP and did not simultaneously sacrifice patient outcomes in other areas

Assessment of 2-Year Storage Conditions on Protein, RNA, and DNA in Unstained Human Tissue Sections, Including a Novel Multiplex Digital Gene Expression Profiling Method with Implications for Biobanking.

Background: Formalin-fixed, paraffin-embedded (FFPE) tissues are a valuable resource for clinical and basic science research. Paraffin blocks and the resulting unstained sections (USS) are often stored for years before being used. Previous studies have evaluated the effects of time, temperature, humidity, and inert gases on preservation of USS; however, no study has examined all four variables together. Methods: In the current work, we prospectively and blindly assessed time points from 0 to 24 months, room versus refrigerated temperature, and presence of a desiccant and/or nitrogen atmosphere on a variety of benign and malignant tissues from North America and Africa. End points included immunohistochemistry (IHC), in situ hybridization (ISH), extracted RNA and DNA quantity and quality, and messenger RNA performance in a novel, multiplexed digital gene expression profiling assay of both housekeeping and tumor-specific genes. Results: We found that using current methods of antigen retrieval, staining, and extraction, the end points of IHC, ISH, RNA, and DNA were well preserved under the various conditions tested, with implications that pre-embedding factors contribute to variability in subsequent tissue integrity. We also document that spectrophotometric estimations of nucleic acid concentrations were in general estimated to be higher than with fluorimetric methods, which may be pertinent to end assay development. We further describe a new multiplex assay, the PlexSet digital gene expression assay, suitable for evaluating RNA quality in FFPE tissues. Conclusion: Altogether, these results may provide helpful guidance with regard to approaches for long-term storage conditions for USS

The Environmental and Ecological Benefits of Green Infrastructure for Stormwater Runoff in Urban Areas

Water runoff from impervious surfaces threatens urban ecosystems, public health and property values. Traditional stormwater management systems are often overwhelmed after big storms, prompting the evaluation of alternative green infrastructure (GI) strategies to improve stormwater management. Here, we present a synthesis to determine the effectiveness of GI— detention basins, filtration devices, bioinfiltration, constructed wetlands, green roofs, and permeable pavement—in reducing runoff volumes and peak flows and in mitigating water pollutant loads by testing and using surrogates such as total suspended solids (TSS) and total nitrogen (TN) from storm runoff. In general, all infrastructures reduced stormwater quantity and/or improved runoff water quality at a local scale, and their performance was comparable to more traditional stormwater management approaches (i.e. detention basins). There was a general agreement between the peer-reviewed data and the best management practice (BMP) database for most GI effectiveness, particularly with respect to water quality. Our analysis shows, however, that the effectiveness of most GI was highly variable, possibly due to climate, influent concentration, or scale. Despite the variability in stormwater runoff performance, most GI can potentially provide valuable habitat for wildlife in urban settings. GI can be designed to promote additional ecosystem services in urban areas, such as habitat for flora or pollinators that can aid in urban gardens or C sequestration, among many others