Bisphosphonate-Functionalized Gold Nanoparticles for Contrast-Enhanced X-Ray Detection of Breast Microcalcifications

Microcalcifications are one of the most common abnormalities detected by mammography for the diagnosis of breast cancer. However, the detection of microcalcifications and correct diagnosis of breast cancer are limited by the sensitivity and specificity of mammography. Therefore, the objective of this study was to investigate the potential of bisphosphonate-functionalized gold nanoparticles (BP-Au NPs) for contrast-enhanced radiographic detection of breast microcalcifications using two models of breast microcalcifications which allowed for precise control over levels of hydroxyapatite (HA) mineral within a low attenuating matrix. First, an in vitro imaging phantom was prepared with varying concentrations of HA uniformly dispersed in an agarose hydrogel. The X-ray attenuation of HA-agarose compositions labeled by BP-Au NPs was increased by up to 26 HU compared to unlabeled compositions for HA concentrations ranging from 1–10 mg/mL. Second, an ex vivo tissue model was developed to more closely mimic the heterogeneity of breast tissue by injecting varying concentrations of HA in a Matrigel carrier into murine mammary glands. The X-ray attenuation of HA-Matrigel compositions labeled by BP-Au NPs was increased by up to 289 HU compared to unlabeled compositions for HA concentrations ranging from 0.5–25 mg/mL, which included an HA concentration (0.5 mg/mL) that was otherwise undetectable by micro-computed tomography. Cumulatively, both models demonstrated the ability of BP-Au NPs to enhance contrast for radiographic detection of microcalcifications, including at a clinically-relevant imaging resolution. Therefore, BP-Au NPs may have potential to improve clinical detection of breast microcalcifications by mammography

Effects of bisphosphonate ligands and PEGylation on targeted delivery of gold nanoparticles for contrast-enhanced radiographic detection of breast microcalcifications

A preclinical murine model of hydroxyapatite (HA) breast microcalcifications (µcals), which are an important clinical biomarker for breast cancer detection, was used to investigate the independent effects of high affinity bisphosphonate (BP) ligands and a polyethylene glycol (PEG) spacer on targeted delivery of gold nanoparticles (Au NPs) for contrast-enhanced radiographic detection. The addition of BP ligands to PEGylated Au NPs (BP-PEG-Au NPs) resulted in five-fold greater binding affinity for targeting HA µcals, as expected, due to the strong binding affinity of BP ligands for calcium. Therefore, BP-PEG-Au NPs were able to target HA µcals in vivo after intramammary delivery, which enabled contrast-enhanced radiographic detection of µcals in both normal and radiographically dense mammary tissues similar to previous results for BP-Au NPs, while PEG-Au NPs did not. The addition of a PEG spacer between the BP targeting ligand and Au NP surface enabled improved in vivo clearance. PEG-Au NPs and BP-PEG-Au NPs were cleared from all mammary glands (MGs) and control MGs, respectively, within 24–48 h after intramammary delivery, while BP-Au NPs were not. PEGylated Au NPs were slowly cleared from MGs by lymphatic drainage and accumulated in the spleen. Histopathology revealed uptake of PEG-Au NPs and BP-PEG-Au NPs by macrophages in the spleen, liver, and MGs; there was no evidence of toxicity due to the accumulation of NPs in organs and tissues compared with untreated controls for up to 28 days after delivery

In Search of the Edge: A Bayesian Exploration of the Detectability of Red Edges in Exoplanet Reflection Spectra

Reflection spectroscopy holds great promise for characterizing the atmospheres and surfaces of potentially habitable terrestrial exoplanets. The surface of the modern Earth exhibits a sharp albedo change near 750 nm caused by vegetation - the red edge - which would leave a strong spectral signature if present on an exoplanet. However, the retrieval of wavelength-dependent surface properties from reflection spectra has seen relatively little study. Here, we propose a new surface albedo parameterization capable of retrieving the wavelength location of a priori unknown 'edge-like' features. We demonstrate that a wavelength-dependent surface albedo model achieves higher accuracy in retrieving atmospheric composition. Wavelength-dependent surfaces are also generally preferred over a uniform albedo model when retrieving simulated reflection spectra for a modern Earth analog, even for moderate signal-to-noise ratios (S/N = 10) and Earth-like clouds. Further, the location of the modern Earth's red edge can be robustly and precisely constrained (within 70 nm for S/N = 10). Our results suggest that future space-based direct imaging missions have the potential to infer surface compositions for rocky exoplanets, including spectral edges similar to those caused by life on the modern Earth.Comment: 23 pages, 17 figures. Accepted for publication in Ap

Parental Anxiety Associated with Summer Camp Experiences: A Comparative Analysis Across Volunteer and Employee-Staffed Camps

Parent anxiety can limit a parent’s willingness to involve their child in out-of-school time experiences such as summer camps. Researchers have studied anxiety within the context of camp, but these studies used narrow frameworks of anxiety. In this exploratory study, we collected open-ended responses about causes of parent anxiety associated with summer camp experiences from 656 parents whose children attended one of two Extension-administered camps. The camps represented different camp staffing models—one primarily staffed by volunteers and the other primarily staffed by employees. The primary purpose of the study was to identify salient categories of anxiety and to examine if anxiety differed based on staffing model. The secondary purpose was to develop a camp-related parent anxiety measure informed by the anxiety categories. Eleven categories were constructed from the data, which both affirmed and expanded existing literature on parent anxiety associated with camp experiences. No differences in parent anxiety were found based on staffing model, suggesting that parents were no more likely to perceive anxiety associated with camp when the program was staffed with volunteers as they were when the program was staffed by employees. Implications for practice and future directions are examined

The impact of the skim milk powder manufacturing process on the flavor of model white chocolate

Milk powder is an important ingredient in the confectionary industry but its variable nature has consequences for the quality of the final confectionary product. This paper demonstrates that skim milk powders (SMP) produced using different (but typical) manufacturing processes, when used as ingredients in the manufacture of model white chocolates, had a significant impact on the sensory and volatile profiles of the chocolate. SMP was produced from raw bovine milk using either low or high heat treatment, and a model white chocolate was prepared from each SMP. A directional discrimination test with naïve panellists showed that the chocolate prepared from the high heat SMP had more caramel/fudge character (p<0.0001), and sensory profiling with an expert panel showed an increase in both fudge (p<0.05) and condensed milk (p<0.05) flavor. GC-MS and GC-Olfactometry of both the SMPs and the model chocolates showed a concomitant increase in Maillard-derived volatiles which are likely to account for this change in flavor

Results of the Kennedy Space Center 50-MHz Doppler Radar Wind Profiler Operational Acceptance Test

No abstract availabl

Results of the Updated NASA Kennedy Space Center 50-MHz Doppler Radar Wind Profiler Operational Acceptance Test

We present here the methodology and results of the Operational Acceptance Test (OAT) performed on the new Kennedy Space Center (KSC) 50-MHz Doppler Radar Wind Profiler (DRWP). On day-of-launch (DOL), space launch vehicle operators have used data from the DRWP to invalidate winds in prelaunch loads and trajectory assessments due to the DRWP's capability to quickly identify changes in the wind profile within a rapidly-changing wind environment. The previous DRWP has been replaced with a completely new system, which needs to undergo certification testing before being accepted for use in range operations. The new DRWP replaces the previous three-beam system made of coaxial cables and a copper wire ground plane with a four-beam system that uses Yagi antennae with enhanced beam steering capability. In addition, the new system contains updated user interface software while maintaining the same general capability as the previous system. The new DRWP continues to use the Median Filter First Guess (MFFG) algorithm to generate a wind profile from Doppler spectra at each range gate. DeTect (2015) contains further details on the upgrade. The OAT is a short-term test designed so that end users can utilize the new DRWP in a similar manner to the previous DRWP during mission operations at the Eastern Range in the midst of a long-term certification process. This paper describes the Marshall Space Flight Center Natural Environments Branch's (MSFC NE's) analyses to verify the quality and accuracy of the DRWP's meteorological data output as compared to the previous DRWP. Ultimately, each launch vehicle program has the responsibility to certify the system for their own use

Results of the NASA Kennedy Space Center 50-MHz Doppler Radar Wind Profiler Operational Acceptance Test

This paper presents results of the new Kennedy Space Center (KSC) 50-MHz Doppler Radar Wind Profiler (DRWP) Operational Acceptance Test (OAT). The goal of the OAT was to verify the data quality of the new DRWP against the performance of the previous DRWP in order to use wind data derived by the new DRWP for space launch vehicle operations support at the Eastern Range. The previous DRWP was used as a situational awareness asset for mission operations to identify rapid changes in the wind environment that weather balloons cannot depict. The Marshall Space Flight Center's Natural Environments Branch assessed data from the new DRWP collected during Jan-Feb 2015 against a specified set of test criteria. Data examination verified that the DRWP provides complete profiles every five minutes from 1.8-19.5 km in vertical increments of 150 m. Analysis of 49 concurrent DRWP and balloon profiles presented root mean square wind component differences around 2.0 m/s. Evaluation of the DRWP's coherence between five-minute wind pairs found the effective vertical resolution to be Nyquist-limited at 300 m for both wind components. In addition, the sensitivity to rejecting data that do not have adequate signal was quantified. This paper documents the data, quality control procedures, methodology, and results of each analysis

High Temporal Resolution Tropospheric Wind Profile Observations at NASA Kennedy Space Center during Hurricane Irma

The National Aeronautics and Space Administration's (NASA) Kennedy Space Center (KSC) operates a 48-MHz Tropospheric/Stratospheric Doppler Radar Wind Profiler (TDRWP) on a continual basis generating wind profiles between 2-19 km (approximately 6.6-62.3 kft) in the support of space launch vehicle operations. A benefit of the continual operability of the system is the ability to provide unique observations of severe weather events such as hurricanes. On the evening of 10 September 2017, Hurricane Irma passed within 160 km (87 nmi) to the west of KSC through the middle of the Florida peninsula. The hurricane was responsible for power outages to approximately 2/3 of Florida's population (Stein, 2017). This paper will provide an overview of the TDRWP system, describe the characteristics of the wind observations from the TDRWP during Irma passage, provide a comparison to previous TDRWP observations from Hurricane Matthew in 2016, and provide the location where TDRWP data is available to the meteorological community