Fast perinuclear clustering of mitochondria in oxidatively stressed human choriocarcinoma cells

Mitochondrial dysfunction plays a crucial role in cell types that exhibit necrosislike death after activation of their death program. Tumour necrosis factor (TNF) induces abnormal, perinuclear clustering of mitochondria from an evenly spread distribution throughout the cytoplasm. The mitochondria withdraw from the cell periphery and aggregate in a unipolar perinuclear cluster. TNF-induced mitochondrial clustering is caused by impaired kinesin-mediated transportation of mitochondria. In this report, we describe a novel activity of menadione (MEN), namely the induction of an altered spatial distribution of mitochondria in the choriocarcinoma JAR cells. Strikingly, 2 hours of cell exposition to menadione did not disrupt the integrity of the plasma membrane, while the intracellular ATP level significantly decreased. Control (untreated) cells displayed a typically scattered distribution of filamentary mitochondria inside the cell. After 2 hours of MEN treatment the spatial distribution of the mitochondria was markedly altered to an asymmetric perinuclear clustered distribution. Menadione-stressed cells displayed a highly asymmetrical perinuclear clustered distribution of the mitochondria. The exposure of cells to MEN also results in a change in shape of the mitochondria into a population of enlarged granular structures. The results of our study demonstrate that in JAR cells menadione causes mitochondria to translocate from the cell periphery into the perinuclear region several hours before disruption of cell membrane integrity and cell death

Multi-Channel Data Acquisition System with Absolute Time Synchronization

A low-cost, stand-alone Global-Positioning-System-time-synchronized data acquisition system is described. The constructed prototype allows recoding up to four analog signals with a 16-bit resolution in variable ranges and a maximum sampling rate of 1000 S/s. Additionally, two digital readouts of external sensors can be acquired. A complete data set is stored on a Secure Digital (SD) card or transmitted to a computer using Universal Serial Bus (USB). The estimated time accuracy of the data acquisition is better than 0.2 {\mu}s. The device is envisioned for the use in a global distributed sensor network (the Global Network of Optical Magnetometers for Exotic physics - GNOME), whose aim is to search for new particles and interactions

Absolute measurement of the ^{1}S_{0} − ^{3}P_{0} clock transition in neutral ^{88}Sr over the 330 km-long stabilized fibre optic link

We report a stability below $7\times 10{}^{-17}$ of two independent optical lattice clocks operating with bosonic ${}^{88}$Sr isotope. The value (429228066418008.3(1.9)${}_{syst}$(0.9)${}_{stat}$~Hz) of the absolute frequency of the ${}^{1}S_{0}$ - ${}^{3}P_{0}$ transition was measured with an optical frequency comb referenced to the local representation of the UTC by the 330 km-long stabilized fibre optical link. The result was verified by series of measurements on two independent optical lattice clocks and agrees with recommendation of Bureau International des Poids et Mesures

Populist Discourse in the Polish Media

Grant MNiSW Narodowy Program Rozwoju Humanistyki (nr 0131/NPRH4/H2b/83/2016

The current possible treatment approaches of Polycystic Ovary Syndrome (PCOS)

Introduction             Polycystic ovary syndrome (PCOS), first described by Stein and Leventhal in 1935, is one of the most prevalent endocrine system conditions affecting women of reproductive age. It affects between 6% and 13% of women and the majority of cases are identified between the ages of 20 and 30. Unfortunately, the disease is usually diagnosed only when bothersome symptoms such as hair loss, alopecia, acne, and infertility-related problems occur. Based on the Rotterdam criteria, four phenotypes of PCOS are distinguished. Aim of the study             This review aims to present the current state of knowledge about possible treatment approaches, both non-pharmacological and pharmacological. Materials and methods            The paper was created based on the Pubmed database. The literature was reviewed using the keywords: ”PCOS”, ”PCOS treatment” and “ PCOS medications “. The current state of knowledge           Treatments for PCOS must be tailored to the specific needs of each patient. In the management of PCOS, special attention is paid to diet, physical activity, and restoration of the Gut Microbiome. Medications used in therapy are oral contraceptives and anti-androgens, insulin sensitizers, ovulation inducers, calcium and vitamin D supplements, statins, Glucagon-like-peptide-1 (GLP-1) agonists, inositols and interleukin 22 (IL-22)  therapy. Summary            Treatment options for menstrual irregularities and hirsutism are based on the clinical goals and preferences of the patient. The ideal would be causal treatment, but due to the ongoing lack of full understanding of the pathogenesis of the syndrome, is not entirely feasible. The ideal would be causal treatment, but due to the ongoing lack of full understanding of the pathogenesis of the syndrome, is not entirely feasible. The most important is a multimodal approach to treat comorbid conditions such as diabetes mellitus type 2, obesity, hyperlipidemia, depression, and infertility

The multimodal approach to obesity treatment – current pharmacological and surgical methods and lifestyle changes

Introduction            Obesity is one of the major health problems of today’s population and is defined as a body mass index ≥ 30 kg/m2.  It is known that obesity may cause many complications such as type 2 diabetes, cardiovascular disease, osteoarthritis, obstructive sleep, apnoea, and several cancers. The only effective treatment of obesity can be pharmacological or surgical, especially when a years-long attempt to change habits has had no effects.  Aim of the study This review aims to present the current state of knowledge about non-pharmacological and pharmacological obesity treatment methods. Materials and methods            The paper was created based on the Pubmed database. The literature was reviewed using the keywords: ”obesity”, ”obesity treatment”, “ obesity lifestyle changes”, “obesity medications” and ”obesity surgery”. The current state of knowledge           The treatment of obesity requires a multimodal approach to treatment, including the addition of anti-obesity medications or bariatric surgery, or both, to assist people in reaching and sustaining sufficient weight loss to meet treatment goals. The 3 principal components of a comprehensive lifestyle intervention are diet, physical activity, and behavioral therapy. Among available anti-obesity medications include orlistat, phentermine, topiramate, naltrexone, bupropion, liraglutide, and semaglutide. Summary            The key challenge in the treatment of obesity is to maintain the effects obtained with multimodal therapy. Without proper motivation of patients and changes in eating and behavioral habits, it is impossible to achieve optimal results, therefore, in addition to medical interventions, more and more attention should be paid to psychological interventions

Analogowa światłowodowa głowica odbiorcza opis patentowy nr 147736 /

Zgłoszono 12 września 1985 r.Zgłoszenie ogłoszono 6 kwietnia 1987 r.Opublikowano 31 stycznia 1990 r.Nr zgłosz. P 255365.Tyt. z ekranu tyt.Dostępny także w wersji drukowanej.Tryb dostępu: Internet

Układ elektroniczny do generacji impulsów sekwencji telefonicznej opis patentowy patentu tymczasowego nr 113365 /

Zgłoszono 20 listopada 1978 r.Zgłoszenie ogłoszono 10 września 1979 r.Opublikowano 30 kwietnia 1982 r.Nr zgłosz. P 211124.Tyt. z ekranu tyt.Dostępny także w wersji drukowanej.Tryb dostępu: Internet

Układ elektroniczny do generacji impulsów sekwencji telefonicznej opis patentowy nr 113365 /

Zgłoszono 20 listopada 1978 r.Zgłoszenie ogłoszono 10 września 1979 r.Opublikowano 1 lipca 1982 r.Nr zgłosz. P 211124.Tyt. z ekranu tyt.Dostępny także w wersji drukowanej.Tryb dostępu: Internet

Strategies for building the image of a fashion brand using Instagram on selected examples

Tematem pracy dyplomowej są strategie budowania wizerunku marki modowej z wykorzystaniem Instagrama, w oparciu o wybrane przykłady. Celem pracy jest zidentyfikowanie kluczowych składników ich koncepcji w tym kanale społecznościowym, w tym typów i częstotliwości publikowanych treści i strategii angażujących użytkowników. Badania dostarczyły wiedzy, że marki streetwearowe, których odbiorcami jest głównie młode pokolenie posługują się Instagramem w celu zarówno publikowania treści, zamieszkania materiałów zdjęciowych oraz filmowych, służących przede wszystkim zaznajamianiu potencjalnych konsumentów z oferowanymi produktami. Istotne znaczenie w strategii marek zajęło również utrzymywanie relacji z klientami, głównie poprzez reakcje, tworzenie wpisów czy udzielanie odpowiedzi na komentarze. Uzyskane wyniki sugerują, że obecnie media społecznościowe są nieodzownym elementem strategii marek modowych, a dostrzeżenie ich znaczenia warunkuje sukces marki, możliwość jej przetrwania, a także stanowi kluczowy determinant jej rozwoju.The topic of the thesis is strategies for building the image of a fashion brand using Instagram, based on selected examples. The aim of the paper is to identify the key components of their concept in this social channel, including the types and frequency of published content and strategies of engaging users. Research has provided knowledge that streetwear brands, whose audience is mainly the young generation, use Instagram to both publish content, inhabit photo and film materials, primarily serving to familiarize potential consumers with the offered products. Maintaining relationships with customers, mainly through reactions, creating posts or responding to comments, has also become an important part of the brand’s strategy. The results suggest that nowadays social media is an indispensable element of fashion brands’ strategies, and the recognition of their importance determines the success of the brand, it is ability to survive, and is also a key determinant of its development