American College of Rheumatology Provisional Criteria for Clinically Relevant Improvement in Children and Adolescents With Childhood-Onset Systemic Lupus Erythematosus

10.1002/acr.23834ARTHRITIS CARE & RESEARCH715579-59

ANHYDROBIOSIS IN YEAST GROWN AT OXYGEN LIMITED CONDITIONS AND ITS BIOTECHNOLOGICAL APPLICATIONS

Promocijas darba gaitā izvērtētas iespējas uzlabot raugu Saccharomyces cerevisiae kultivētu limitēta skābekļa apstākļos izturību dehidratācijas-rehidratācijas procesā. Izpētīta dažādu vielu - antioksidantu, cukuru, sāls, poliolu - iespējamā ietekme uz šūnu rezistences palielināšanu. Tāpat parādīta iespēja izmantot celulozi saturošu substrātu pēc furfurola izdalīšanas etanola sintēzei. Pētījumos noskaidroti apstākļi enzimātiskās hidrolīzes procesam un papildus priekšapstrādes izmantošanas efektivitāte glikozes iznākuma uzlabošanai, kā arī iegūtā hidrolizāta un dehidratēto raugu izmantošanas iespējas. Promocijas darbā novērotās likumsakarības raugu šūnu atbildes reakcijās uz inkubācijas priekšapstrādi izmantotas biopesticīdu producentu Pseudomonas aurantiaca fizioloģiskajai inženierijai. Atslēgas vārdi: Saccharomyces cerevisiae, anhidrobioze, bioetanolsDuring doctoral thesis, there were examined ways to improve endurance of yeast Saccharomyces cerevisiae cultivated at oxygen limited conditions in the process of dehydration-rehydration. There were researched possible effects of various substances - antioxidants, sugars, salt, polyols - on increasing cell resistance. Also there has been shown a way to use cellulose-containing substrate after furfural extraction for ethanol production. Studies identified conditions for enzymatic hydrolysis process and the effectiveness of the use of additional pre-treatment to improve the outcome of glucose as well as possibilities of use of hydrolysate and dehydrated yeast. The observed regularities of yeast cell responses to incubation pre-treatment were used in physiological engineering of biopesticide producers Pseudomonas aurantiaca. Keywords: Saccharomyces cerevisiae, anhydrobiosis, bioethano

Physiological engineering of Pseudomonas aurantiaca antimicrobial activity: effects of sodium chloride treatment

The influence of sodium chloride (NaCl) treatment on the antifungal activity of the bacterium <em>Pseudomonas aurantiaca</em>, a producer of biopesticide for vegetable plants, was investigated. It was shown that an increase in the NaCl concentration in incubation solutions from 1 M to 3 M led to a significant increase in the antifungal activity of this bacterium. Antifungal activity continued to increase with prolonged treatment of bacteria in fresh nutrient medium from 72 h to 96 h. These findings could be very important for the further development of biotechnological processes directed not only to the production of new active biopesticides but also of other valuable resources

Anhydrobiosis in yeasts: Glutathione synthesis by yeast Ogataea (Hansenula) polymorpha cells after their dehydration-rehydration

The possibility of using active dry microbial preparations in biotechnological processes is essential for the development of new modern industrial technologies. In this study, we show the possibility of obtaining such preparations of the genetically engineered yeast strain Ogataea (Hansenula) polymorpha with glutathione overproduction. Special pre-treatment involving the gradual rehydration of dry cells in water vapour led to the restoration/reactivation of almost 100% of dehydrated cells. Furthermore, dry cells do not lose their viability during storage at room temperatures. Application of dry cells as the inoculum provides the same levels of glutathione synthesis as that of a native yeast cultureBiochemijos katedraVytauto Didžiojo universiteta

American college of rheumatology provisional criteria for clinically relevant improvement in children and adolescents with childhood-onset systemic Lupus erythematosus

To develop a Childhood Lupus Improvement Index (CHILI) as a tool to measure response to therapy in childhood-onset systemic lupus erythematosus (cSLE), with a focus on clinically relevant improvement (CRIcSLE). Methods Pediatric nephrology and rheumatology subspecialists (n = 213) experienced in cSLE management were invited to define CRIcSLE and rate a total of 433 unique patient profiles for the presence/absence of CRIcSLE. Patient profiles included the following cSLE core response variables (CRVs): global assessment of patient well-being (patient-global), physician assessment of cSLE activity (MD-global), disease activity index score (here, we used the Systemic Lupus Erythematosus Disease Activity Index), urine protein-to-creatinine ratio, and Child Health Questionnaire physical summary score. Percentage and absolute changes in these cSLE-CRVs (baseline versus follow-up) were considered in order to develop candidate algorithms and validate their performance (sensitivity, specificity, area under the receiver operating characteristic curve [AUC]; range 0-1). Results During an international consensus conference, unanimous agreement on a definition of CRIcSLE was achieved; cSLE experts (n = 13) concurred (100%) that the preferred CHILI algorithm considers absolute changes in the cSLE-CRVs. After transformation to a range of 0-100, a CHILI score of >= 54 had outstanding accuracy for identifying CRIcSLE (AUC 0.93, sensitivity 81.1%, and specificity 84.2%). CHILI scores also reflect minor, moderate, and major improvement for values exceeding 15, 68, and 92, respectively (all AUC >= 0.92, sensitivity >= 93.1%, and specificity >= 73.4%). Conclusion The CHILI is a new, seemingly highly accurate index for measuring CRI in cSLE over time. This index is useful to categorize the degree of response to therapy in children and adolescents with cSLE.715579590CNPQ - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPESP – Fundação de Amparo à Pesquisa Do Estado De São Paulo303422/2015-7; 7/2016-9; 304255/2015-7215/03756-