Efektivitas Mikroorganisme Lokal (Mol) Kulit Pisang Dan Bonggol Pisang Terhadap Pertumbuhan Tanaman Selada (Lactuca sativa L) Pada Media Hidroponik

The content contained on the banana weevil are calcium, phosphorus, carbohydrates, protein, iron, while the banana skin contain nitrogen, carbohydrates and others that are necessary plant. Weevil bananas and banana skin can be applied as a liquid fertilizer or MOL (Local Microorganisms). This study aims to determine the effectiveness of the banana weevil MOL and banana skin on the growth of lettuce plants with hydroponics media. This study uses quantitative methods experiment with completely randomized design (CRD) two factors: the type of MOL (Q0: plain water, Q1: banana weevil, Q2: banana skin) and the concentration of MOL (P1: Concentration 18%, Q2: 24% concentration , P3: concentration 30%) and 9 treatments and 3 replications. Analysis of the data used is two way anova at the level of 5% F, known concentrations of MOL no significant effect on growth (stem length, number of leaves, leaf width), while the types of MOL significantly to the growth of lettuce plants. To know the real difference between the treatments tested the Least Significant Difference Q1P2 is 2.16 cm, number of leaves Q2P2 is 2, and the leaves are Q1P2 width of 1.5 cm

In GFP with high risk HPV-18E6 fusion protein expressed 293T and MCF-7 cells, the endogenous wild-type p53 could be transiently phosphorylated at multiple sites

<p>Abstract</p> <p>Background</p> <p>Infected cells recognize viral replication as a DNA damage stress and elicit the host surveillance mechanism to anti-virus infection. Modulation of the activity of tumor suppressor p53 is a key event in the replication of many viruses. They could manipulate p53 function through phosphorylation modification for their own purpose. But there is rarely research about p53 phosphorylation status in the context of HPV-E6. Therefore, we investigated whether p53 could be phosphorylated by HPV-E6.</p> <p>Methods</p> <p>We used a mammalian green fluorescence protein (GFP) expression system to express HPV-18E6 with GFP fusion proteins (GFP-18E6) in wild-type (wt) p53 cell lines, such as 293T and MCF-7 cells to trace the traffic and subcellular location of E6 protein. By immunofluorescence technique and immunoblotting, we determined the positive phosphorylated sites of p53 and observed the distribution of phosphorylated p53 in the context of GFP-18E6.</p> <p>Results</p> <p>GFP-18E6 was predominantly located in nuclei of wt p53 cell lines, and it could induce transient phosphorylation of p53 at multiple sites, such as Ser¹⁵, Ser²⁰, and Ser³⁹². All the three sites of phosphorylated p53s were localized in nuclei together with GFP-18E6.</p> <p>Conclusion</p> <p>In GFP with high risk HPV-18E6 fusion protein expressed 293T and MCF-7 cells, the endogenous wt p53 could be transiently phosphorylated at multiple sites.</p

The association between umbilical cord blood fat-soluble vitamin concentrations and infant birth weight

BackgroundFat-soluble vitamins, including vitamins A, D and E, play an important role in the regulation of glucose and lipid metabolism, and may affect infant birth weight. Evidence on the association of birthweight with fat-soluble vitamins is controversial. Therefore, this study aims is to determine the associations of birthweight with vitamin A, D, and E concentrations in cord blood.MethodsA total of 199 mother–infant pairs were enrolled in the study. According to gestational age and birth weight, the mother–infant pairs were divided into small for gestational age (SGA), appropriate for gestational age (AGA), and large for gestational age (LGA). The Vitamin A, D, and E concentrations in serum were measured by high-performance liquid chromatography tandem-mass spectrometry.ResultsThe concentrations of vitamin A in the SGA group were significantly lower than those in the AGA and LGA groups. The concentrations of vitamin E in the SGA group were significantly higher than those in the AGA and LGA groups. However, no significant differences were observed in vitamin D among the three groups. Being male (β = 0.317, p &lt; 0.001) and birth weight (β = 0.229, p = 0.014) were positively correlated with the levels of vitamin A. Birth weight (β = -0.213, p= 0.026) was correlated with lower levels of vitamin E. No correlation was found between influencing Factors and the levels of vitamin D (p&gt; 0.05). After adjusting for gestational age, sex, mother’s age, delivery mode, pre-pregnancy BMI, and weight gain during pregnancy, the levels of cord blood vitamin A were positively correlated with birth weight (p=0.012).ConclusionThe infant’s birth weight is associated with the levels of cord blood vitamins A and E. The dysregulation of vitamins A and E in infants may be a risk factor for fetal growth and future metabolic diseases

Pengaruh Konservatisme Akuntansi, Intensitas Aset Tetap, Profitabilitas, Winner, Dan Debt To Equity Ratio Terhadap Tax Avoidance (Studi Empiris pada Perusahaan Manufaktur Yang Terdaftar di Bursa Efek Indonesia (BEI)Tahun 2017-2019)

This study aims to discuss accounting conservatism, Fixed Asset Intensity, Profitability, Winner and Debt To Equity Ratio Against Tax Avoidance (Empirical Study of Manufacturing companies listed on the Indonesia Stock Exchange (BEI) 2017-2019) The type of research used is associative research. and the type of research used is quantitative. Sampling in this study was carried out using purposive sampling method. This research was conducted by selecting public companies listed on the Indonesia Stock Exchange and the samples used were manufacturing companies listed on the Indonesia Stock Exchange in 2017-2019. The sample used by 75 manufacturing companies listed on the Indonesia Stock Exchange in 2017-2019. The type of data used is secondary data. Data analysis used logistic regression analysis. The results show that conservatism does not conflict with tax avoidance

PENGARUH INSTRUMEN MONETER SYARIAH TERHADAP TINGKAT INFLASI DI INDONESIA

Perekonomian Indonesia tidak terlepas dari permasalahan inflasi, kondisi tingkat inflasi dengan menggunakan Indeks Harga Konsumen (IHK) pada periode tahun 2011-2018 mengalami perkembangan yang fluktuatif dengan tren penurunan. Dalam menjalankan tugas moneter Bank Indonesia membuat standar ukuran keberhasilan pencapaian tingkat inflasi yang tercermin dalam inflation targeting framework. Selalu terjadi kesenjangan antara aktual inflasi dengan target yang telah ditetapkan. Oleh karena itu, diperlukan penggunaan instrumen kebijakan moneter yang tepat yang berdasarkan prinsip keadilan dan persaudaraan guna mengatur jumlah uang yang beredar yang kemudian akan berpengaruh terhadap tingkat inflasi. Penelitian ini bertujuan untuk melihat pengaruh dari instrumen kebijakan moneter syariah, yaitu Sertifikat Bank Indonesia Syariah (SBIS), Giro Wajib Minimum (GWM), dan Fasilitas Simpanan Bank Indonesia Syariah (FASBIS) dalam mengendalikan tingkat inflasi di Indonesia pada periode Januari 2011 hingga Maret 2019. Penelitian ini menggunakan alat analisis Vector Error Correction Model (VECM). Hasil penelitian menunjukkan bahwa pada jangka pendek semua variabel tidak berpengaruh secara signifikan terhadap tingkat inflasi. Dalam jangka panjang SBIS tidak berpengaruh secara signifikan terhadap tingkat inflasi. Kemudian, GWM berpengaruh negatif dan signifikan terhadap tingkat inflasi dalam jangka panjang. Adapun FASBIS berpengaruh positif dan signifikan terhadap tingkat inflasi dalam jangka panjang. Kata Kunci: Kebijakan Moneter Syariah, Tingkat inflasi, SBIS, GWM, FASBIS. The Indonesian economy is inseparable from inflation issues, the inflation-level condition using the Consumer Price Index (CPI) in period 2011-2018 underwent fluctuating developments with a downward trend. In the context of carrying out monetary tasks, Bank Indonesia has set achievement standards that support the inflation targeting framework. There is always a gap between the actual inflation and the specified target. Therefore, it is necessary to use proper monetary policy instruments based on the principles of fairness and brotherhood in order to regulate the circulation amount of money which will then affect the inflation rate. This study aims to look at the influence of sharia monetary policy, namely Bank Indonesia Certificate Sharia (SBIS), Legal Reserve Requirement (GWM), and Bank Indonesia Deposit Facility Sharia (FASBIS) in controlling the rate of inflation in Indonesia from January 2011 to March 2019. This study uses the Vector Error Correction Model (VECM) analysis method. The results showed that on short term all the variables had no significant effect on the inflation rate. In the long run SBIS is not significant effect on the inflation rate. The GWM then negative affects and significant againts the rate of inflation in the long term. As for the FASBIS effect positive and significant against the rate of inflation in the long term. Keywords: Sharia Monetary Policy, Inflation, SBIS, GWM, FASBIS

SOX2 Gene Regulates the Transcriptional Network of Oncogenes and Affects Tumorigenesis of Human Lung Cancer Cells

Recent studies demonstrated that cancer stem cells (CSCs) have higher tumorigenesis properties than those of differentiated cancer cells and that transcriptional factor-SOX2 plays a vital role in maintaining the unique properties of CSCs; however, the function and underlying mechanism of SOX2 in carcinogenesis of lung cancer are still elusive. This study applied immunohistochemistry to analyze the expression of SOX2 in human lung tissues of normal individuals as well as patients with adenocarcinoma, squamous cell carcinoma, and large cell and small cell carcinoma and demonstrated specific overexpression of SOX2 in all types of lung cancer tissues. This finding supports the notion that SOX2 contributes to the tumorigenesis of lung cancer cells and can be used as a diagnostic probe. In addition, obviously higher expression of oncogenes c-MYC, WNT1, WNT2, and NOTCH1 was detected in side population (SP) cells than in non-side population (NSP) cells of human lung adenocarcinoma cell line-A549, revealing a possible mechanism for the tenacious tumorigenic potential of CSCs. To further elucidate the function of SOX2 in tumorigenesis of cancer cells, A549 cells were established with expression of luciferase and doxycycline-inducible shRNA targeting SOX2. We found silencing of SOX2 gene reduces the tumorigenic property of A549 cells with attenuated expression of c-MYC, WNT1, WNT2, and NOTCH1 in xenografted NOD/SCID mice. By using the RNA-Seq method, an additional 246 target cancer genes of SOX2 were revealed. These results present evidence that SOX2 may regulate the expression of oncogenes in CSCs to promote the development of human lung cancer

Biocrust reduces the soil erodibility of coral calcareous sand by regulating microbial community and extracellular polymeric substances on tropical coral island, South China Sea

Tropical coral islands assume a pivotal role in the conservation of oceanic ecosystem biodiversity. However, their distinctive environmental attributes and limited vegetation render them highly susceptible to soil erosion. The biological soil crust (biocrust), owing to its significant ecological role in soil stabilization and erosion prevention, is deemed an effective means of mitigating soil erosion on coral island. However, existing research on the mechanisms through which biocrusts resist soil erosion has predominantly concentrated on arid and semi-arid regions. Consequently, this study will specifically delve into elucidating the erosion-resistant mechanisms of biocrusts in tropical coral island environments, South China Sea. Specifically, we collected 16 samples of biocrusts and bare soil from Meiji Island. High-throughput amplicon sequencing was executed to analyze the microbial community, including bacteria, fungi, and archaea. Additionally, quantitative PCR was utilized to assess the abundance of the bacterial 16S rRNA, fungal ITS, archaeal 16S rRNA, and cyanobacterial 16S rRNA genes within these samples. Physicochemical measurements and assessments of extracellular polymeric substances (EPSs) were conducted to characterize the soil properties. The study reported a significantly decreased soil erodibility factor after biocrust formation. Compared to bare soil, soil erodibility factor decreased from 0.280 to 0.190 t h MJ−1 mm−1 in the biocrusts. Mechanistically, we measured the microbial EPS contents and revealed a negative correlation between EPS and soil erodibility factor. Consistent with increased EPS, the abundance of bacteria, fungi, archaea, and cyanobacteria were also detected significantly increased with biocrust formation. Correlation analysis detected Cyanobacteria, Chloroflexi, Deinococcota, and Crenarchaeota as potential microbials promoting EPSs and reducing soil erosion. Together, our study presents the evidence that biocrust from tropical coral island in the South China Sea promotes resistance to soil erosion, pinpointing key EPSs-producing microbials against soil erosion. The findings would provide insights for island soil restoration

Chemical, functional, and structural properties of spent coffee grounds and coffee silverskin

Spent coffee grounds (SCG) and coffee silverskin (CS) represent a great pollution hazard if discharged into the environment. Taking this fact into account, the purpose of this study was to evaluate the chemical composition, functional properties, and structural characteristics of these agro-industrial residues in order to identify the characteristics that allow their reutilization in industrial processes. According to the results, SCG and CS are both of lignocellulosic nature. Sugars polymerized to their cellulose and hemicellulose fractions correspond to 51.5 and 40.45 % w/w, respectively; however, the hemicellulose sugars and their composition significantly differ from one residue to another. SCG and CS particles differ in terms of morphology and crystallinity, but both materials have very low porosity and similar melting point. In terms of functional properties, SCG and CS present good water and oil holding capacities, emulsion activity and stability, and antioxidant potential, being therefore great candidates for use on food and pharmaceutical fields.The authors acknowledge the financial support of the Science and Technology Foundation of Portugal (FCT) through the grant SFRH/BD/80948/2011 and the Strategic Project PEst-OE/EQB/LA0023/2013. The authors also thank the Project "BioInd - Biotechnology and Bioengineering for improved Industrial and Agro-Food processes", REF. NORTE-07-0124-FEDER-000028 co-funded by the Programa Operacional Regional do Norte (ON.2-O Novo Norte), QREN, FEDER. Thanks are also given to Prof. Jose J.M. Orfao, from the Department of Chemical Engineering, Universidade do Porto (Portugal), for his assistance with the porosity analyses