Familial thymic aplasia - Attempted reconstitution with fetal thymus in a Millipore diffusion chamber

A 10-week-old female infant with familial congenital thymic aplasia without delayed hypersensitivity to common skin-test antigens underwent fetal-thymus implantation. Six hours after the implantation of a fetal thymus enclosed in a Millipore chamber phytohemagglutinin responsiveness was demonstrable in the patient's peripheral lymphocytes. The infant's death of aspiration pneumonia nine days after implantation did not allow evaluation of the extent of the immunologic reconstitution. Thymic-cell immunologic function can be induced in man with fetal-thymus humoral factors

Degradabilidade in situ das frações fibrosas da silagem de sorgo.

O objetivo deste experimento foi estudar a degradabilidade in situ e a cinética de degradação da fibra em detergente neutro (FDN) e fibra em detergente ácido (FDA) das silagens de quatro genótipos de sorgo, com presença e ausência de tanino nos grãos. Foi utilizado um delineamento experimental de blocos ao acaso, em esquema de parcelas subdivididas, sendo os animais os blocos, as silagens as parcelas e os tempos de incubação as subparcelas. As médias foram comparadas pelo teste SNK, a 5% de probabilidade. Após 96 horas de incubação, as degradabilidades da FDN e FDA variaram de 32,22 a 56,07% e de 26,40 a 54,40%, para as silagens do BR700 e CMSXS165, respectivamente. A presença de tanino nos grãos comprometeu a degradabilidade ruminal da FDN e FDA das silagens de sorgo

Reproductive and productive behavior of hair sheep under an intensive production system

Objective: To evaluate the reproductive and productive behavior of four hair sheep breeds in an intensive production system. Design/Methodology/Approach: Data recorded over 4 years (2016-2019) from a production unit that works with Blackbelly, Pelibuey, Dorper, and Katahdin breeds were analyzed. The following variables were evaluated: fertility, prolificacy, birth weight (BW), weaning weight (WW), weaned lamb yield (WLY), daily weight gain during lactation (DWGL), offspring born alive (OBA), male offspring (MO), and female offspring (FO). All information was analyzed using Chi-square tests and analysis of variance. Results: The fertility, BW, WW, and WYL variables recorded different values between breeds (P<0.05). The greatest fertility and prolificacy were recorded by Blackbelly, while Dorper recorded the lowest values (P<0.05). BW and WW were highest in Dorper and lowest in Blackbelly (P<0.05). Finally, the sheep that recorded the highest WYL were the Blackbelly, while the Dorper registered the lowest values (P<0.05). BW and DWGL values reached maximum values (P<0.05) in Dorper rams, followed by Katahdin. The greatest number of OBA was observed in Blackbelly and Pelibuey (P<0.05). Study Limitations/Implications: Further studies should be carried out to validate this research. Findings/Conclusions: Blackbelly ewes produced more kilograms of weaned lambs per lambing ewe. The Dorper and Katahdin rams in the pre-weaning period recorded higher weight at weaning

Yield and nutritive value of Urochloa hybrids at different regrowth ages

Objective: To evaluate the productive behavior of Urochloa hybrids, depending on the regrowth age. Design/Methodology/Approach: The study was carried out under rainfed conditions during 2018. The Cayman, Mulato II, Convert 330, Cobra, Camello I, and Camello II hybrids were evaluated based on the regrowth age (2, 4, 6, 8, and 10 weeks). The following variables were evaluated: plant height (PH), total dry matter (TDM) yield, dry matter per leaf (DMl) yield, dry matter per stem (DMs) yield, crude protein content (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF). The data obtained were evaluated by means of a randomized complete block design with three repetitions, divided into plots: a large plot for the cultivars and small plot for the regrowth ages. Results: The Camello II cultivar obtained the highest TDM yields during the sixth and eighth weeks (4.15 and 6.35 t DM ha-1, respectively); however, during the tenth week, the yield was equal to the yield obtained with the Mulato II and Cayman cultivars (p<0.05). The highest DMl yield was obtained by the Mulato II cultivar during the sixth, eighth, and tenth weeks (3.37, 4.56, and 3.86 t DM ha-1, respectively). The Mulato II cultivar recorded the highest CP values during the second and fourth weeks (158 and 126 g kg-1, respectively); however, the Camello II cultivar obtained the highest CP values during the sixth, eighth, and tenth weeks (99, 95, and 87 g kg-1, respectively). The NDF and ADF values increased as the regrowth age increased: in the tenth week, the Camello II and Cobra cultivars obtained the highest NDF values, while the Camello II cultivar recorded the highest ADF value during the same period. Study Limitations/Implications: Urochloa cultivars were developed for their establishment in humid tropical conditions, where their productive performance is greater. However, in dry tropical conditions, the Cayman, Mulato II, and Cobra cultivars have had a better performance than other grasses —such as buffel grass (Pennisetum ciliare), which is used to feed ruminants. In this sense, other Urochloa cultivars (e.g., Camello I and Camello II), which have greater tolerance to droughts, show desirable forage characteristics, such as yield and forage quality. Findings/Conclusions: The cultivars with the best productive performance were Camello II, Mulato II, and Cayman

Abscess formation of a spherical-shape duplication in the splenic flexure of the colon: case report and review of the literature

Gastrointestinal tract duplications are rare congenital malformations that may occur anywhere in the alimentary tract from the mouth to the anus, and vary greatly in presentation, size, location, and especially in symptoms. We present a case of an infected spherical colonic duplication, in a 20-day-old baby, located at the splenic flexure of the colon. The prominent symptom was acute abdomen, accompanied by bilious vomiting, intestinal obstruction, and high fever. We present this case, due to atypical clinical presentation and the inability of the imaging modality to establish the diagnosis preoperatively

DNA Methylation of the ABO Promoter Underlies Loss of ABO Allelic Expression in a Significant Proportion of Leukemic Patients

Background: Loss of A, B and H antigens from the red blood cells of patients with myeloid malignancies is a frequent occurrence. Previously, we have reported alterations in ABH antigens on the red blood cells of 55% of patients with myeloid malignancies. Methodology/Principal Findings: To determine the underlying molecular mechanisms of this loss, we assessed ABO allelic expression in 21 patients with ABH antigen loss previously identified by flow cytometric analysis as well as an additional 7 patients detected with ABH antigen changes by serology. When assessing ABO mRNA allelic expression, 6/12 (50%) patients with ABH antigen loss detected by flow cytometry and 5/7 (71%) of the patients with ABH antigen loss detected by serology had a corresponding ABO mRNA allelic loss of expression. We examined the ABO locus for copy number and DNA methylation alterations in 21 patients, 11 with loss of expression of one or both ABO alleles, and 10 patients with no detectable allelic loss of ABO mRNA expression. No loss of heterozygosity (LOH) at the ABO locus was observed in these patients. However in 8/11 (73%) patients with loss of ABO allelic expression, the ABO promoter was methylated compared with 2/10 (20%) of patients with no ABO allelic expression loss (P = 0.03). Conclusions/Significance: We have found that loss of ABH antigens in patients with hematological malignancies is associated with a corresponding loss of ABO allelic expression in a significant proportion of patients. Loss of ABO allelic expression was strongly associated with DNA methylation of the ABO promoter.Tina Bianco-Miotto, Damian J. Hussey, Tanya K. Day, Denise S. O'Keefe and Alexander Dobrovi

Integrating whole-genome sequencing within the National Antimicrobial Resistance Surveillance Program in the Philippines

Funding: This work was funded by the Newton Fund, Medical Research Council (UK) grant MR/N019296/1, Philippine Council for Health Research and Development project number FP160007. J.S. was partially supported by research grants RR025040 and U01CA207167 from the National Institutes of Health (NIH). S.A. and D.M.A. were additionally supported by the National Institute for Health Research (UK) Global Health Research Unit on genomic Surveillance of AMR(16_136_111) and by the Centre for Genomic Pathogen Surveillance (http://pathogensurveillance.net).National networks of laboratory-based surveillance of antimicrobial resistance (AMR) monitor resistance trends and disseminate these data to AMR stakeholders. Whole-genome sequencing (WGS) can support surveillance by pinpointing resistance mechanisms and uncovering transmission patterns. However, genomic surveillance is rare in low- and middle-income countries. Here, we implement WGS within the established Antimicrobial Resistance Surveillance Program of the Philippines via a binational collaboration. In parallel, we characterize bacterial populations of key bug-drug combinations via a retrospective sequencing survey. By linking the resistance phenotypes to genomic data, we reveal the interplay of genetic lineages (strains), AMR mechanisms, and AMR vehicles underlying the expansion of specific resistance phenotypes that coincide with the growing carbapenem resistance rates observed since 2010. Our results enhance our understanding of the drivers of carbapenem resistance in the Philippines, while also serving as the genetic background to contextualize ongoing local prospective surveillance.Publisher PDFPeer reviewe