A afetividade contribuindo para adaptação e desenvolvimento das crianças do Infantil I do Centro de Educação Infantil Padre José Maria Cavalcante Costa

This experience report brings together observations and actions that took place in the kindergarten class I of  early childhood education center Padre José Maria Cavalcante Costa in the first half of 2023, whose content was based on affectivity as an important strategy for the adaptation and development of children. Aiming to analyze how affectivity can contribute to the adaptation and development of children's autonomy in the Kindergarten I class in the city of Fortaleza-CE. The research was of the bibliographic and quantitative qualitative type and found that the families agreed that affection made a big difference for the children's adaptation and made them more affectionate and less aggressive, in addition to providing a safer and more welcoming environment. Therefore, it is necessary for teachers and schools to work more on affectivity in the school environment, so that children can learn about values, respect for others and solidarity.O presente relato de experiência reúne observações e ações que ocorreram na turma do infantil I do Centro de Educação Infantil Padre José Maria Cavalcante Costa no primeiro semestre de 2023 cujo conteúdo foi baseado na afetividade como uma estratégia importante para a adaptação e o desenvolvimento das crianças. Tendo como objetivo analisar como a afetividade pode contribuir para a adaptação e desenvolvimento da autonomia das crianças na turma do Infantil I no município de Fortaleza-CE. A pesquisa foi do tipo bibliográfica e quanti qualitativa e constatou que as famílias concordavam que a afetividade fez uma grande diferença para a adaptação das crianças e as deixou mais carinhosas e menos agressivas, além de proporcionar um ambiente mais seguro e acolhedor. Portanto, se faz necessário que professores e escola trabalhem mais a afetividade no ambiente escolar, a fim de que as crianças possam aprender sobre valores, respeito pelo outro e solidariedade

Egg quality of laying hens in different conditions of storage, ages and housing densities

The objective of this research was to evaluate the quality of eggs from Dekalb White hens under different temperatures (room temperature, 25 ºC; and refrigeration, 8 ºC), storage times (7, 14 and 21 days), age of birds (35, 40, 45 and 50 weeks) and housing densities (625, 500, 416.6 and 357.14 cm²/birds). Haugh unit (HU) values and albumen height of 1,120 eggs from 528 laying hens, randomly allocated to four groups with different population densities were evaluated. The results showed a significant effect on Haugh units, between the time and storage conditions and between the ages of laying hens and storage condition. Eggs stored in refrigeration showed less weight loss and better Haugh unit indices when compared with eggs stored at room temperature. The increased time of storage, regardless of the temperature, caused loss in their quality. According to the results of this study, based on the evaluation of the albumen height, temperature, storage time, density and age of the layers influence the values of Haugh unit

Cytoxicity and Apoptotic Mechanism of Ruthenium(II) Amino Acid Complexes in Sarcoma-180 Tumor Cells

Over the past several decades, much attention has been focused on ruthenium complexes in antitumor therapy. Ruthenium is a transition metal that possesses several advantages for rational antitumor drug design and biological applications. In the present study, five ruthenium complexes containing amino acids were studied in vitro to determine their biological activity against sarcoma-180 tumor cells. The cytotoxicity of the complexes was evaluated by an MTT assay, and their mechanism of action was investigated. The results demonstrated that the five complexes inhibited the growth of the S180 tumor cell line, with IC50 values ranging from 22.53 mu M to 50.18 mu M, and showed low cytotoxicity against normal L929 fibroblast cells. Flow cytometric analysis revealed that the [Ru(gly)(bipy)(dppb)] PF6 complex (2) inhibited the growth of the tumor cells by inducing apoptosis, as evidenced by an increased number of Annexin V-positive cells and G0/G1 phase cell cycle arrest. Further investigation showed that complex 2 caused a loss of mitochondrial membrane potential; activated caspases 3, caspase-8, and caspase-9 and caused a change in the mRNA expression levels of caspase 3, caspase-9 as well as the bax genes. The levels of the pro-apoptotic Bcl-2 family protein Bak were increased. Thus, we demonstrated that ruthenium amino acid complexes are promising drugs against S180 tumor cells, and we recommend further investigations of their role as chemotherapeutic agents for sarcomas.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Effects of complex 2 on the cell cycle distribution of S180 cells after 24 h (A) and 48 h (B) of exposure.

<p>The data are the means ± SD of three experiments. Significant differences from the untreated control are indicated by ***p<0.001.</p

Effect of complex 2 on the mechanism of S180 cell death (early and late apoptosis/necrosis) evaluated by flow cytometry after 24 h (A) and 48 h (B) of incubation.

<p>The data are the means ± SD of three experiments. Significant differences from the untreated control are indicated by **p<0.01 and ***p<0.001.</p

Effect of complex 2 on the mitochondrial membrane potential.

<p>The percentages of cells with depolarized mitochondrial membrane potential mitochondrial membrane potentials of cells treated with 40 µM and 60 µM complex 2 as well as control cells were assessed by flow cytometry after staining with JC-1. The data are expressed as the means ± SD of two individual experiments; ***p<0.001 compared with the control.</p

Effect of complex 2 on the activity of caspase -3, -8 and -9 in S180 cells.

<p>S180 cells were treated with the complex at the indicated concentrations for 24 h. The data are expressed as the means ± SD of two individual experiments; *p<0.05 and **p<0.01 compared with the control.</p

Viability of the S180 tumor cell line and the L929 normal cell line in the presence of ruthenium amino acid complexes and cisplatin.

<p>Viability of the S180 tumor cell line and the L929 normal cell line in the presence of ruthenium amino acid complexes and cisplatin.</p

Primer sequences used for the real time RT-qPCR assay.

<p>Primer sequences used for the real time RT-qPCR assay.</p