Anti-inflammatory, antinociceptive, and antipyretic effects of methanol extract of Cariniana rubra stem bark in animal models

Schmeda-Hirschmann, G (Schmeda-Hirschmann, Guillermo) Univ Talca, IQRN, Talca, ChileCariniana rubra Miers (Lecythidaceae), popularly known as "jequitiba-vermelho", is a large Brazilian tree whose bark is used in infusion and decoction for the treatment of inflammatory conditions. This study aims to assess the anti-inflammatory, antinociceptive, and antipyretic effects of Cariniana rubra methanolic stem bark extract (EMCr) using experimental animals. Anti-inflammatory activity of EMCr was tested on carrageenan and dextran-induced rat paw edema, carrageenan-induced pleurisy in rats and acetic acid-increase vascular permeability in mice. Antinociceptive and antipyretic activities were evaluated using acetic acid-induced writhing, formalin and hot-plate tests in mice, as well as brewer's yeast-induced pyrexia in rats. The extract inhibitied carrageenan and dextran-induced edema, reduced exudate volume and leukocyte migration on the carrageenan-induced pleurisy and on the vascular permeability increase induced by acetic acid. The EMCr inhibited nociception on the acetic acid-induced writhing and in the second phase of formalin test, and decreased rectal temperature. It was, however, inactive against thermal nociception. Phytochemical analysis with EMCr showed the occurrence of saponins, triterpenes, sterols and phenolic compounds. Phytosterols (beta-sitosterol, stigmasterol), pentacyclic triterpenes (alpha- and beta-amyrin as a mixture), arjunolic acid, a phytosterol glycoside (sitosterol 3-O-beta-D-glucopyranoside), and triterpenoid saponins (28-beta-glucopyranosyl-23-O-acetyl arjunolic acid; 3-O-beta-glucopyranosyl arjunolic acid and 28-O-[alpha-L-Rhamnopyranosyl-(1 -> 2)-beta-glucopyranosyl]-23-O-acetyl arjunolic acid) were the main identified compounds. It can be presumed that EMCr caused their effects by inhibiting the liberation and/or action of different inflammatory mediators. These findings support the traditional use of Cariniana rubra preparations to treat inflammation

CDM Accelerating Cosmology as an Alternative to LCDM model

A new accelerating cosmology driven only by baryons plus cold dark matter (CDM) is proposed in the framework of general relativity. In this model the present accelerating stage of the Universe is powered by the negative pressure describing the gravitationally-induced particle production of cold dark matter particles. This kind of scenario has only one free parameter and the differential equation governing the evolution of the scale factor is exactly the same of the $\Lambda$CDM model. For a spatially flat Universe, as predicted by inflation ($\Omega_{dm}+\Omega_{baryon}=1$), it is found that the effectively observed matter density parameter is $\Omega_{meff} = 1- \alpha$, where $\alpha$ is the constant parameter specifying the CDM particle creation rate. The supernovae test based on the Union data (2008) requires $\alpha\sim 0.71$ so that $\Omega_{meff} \sim 0.29$ as independently derived from weak gravitational lensing, the large scale structure and other complementary observations.Comment: 6 pages, 3 figure

An Accelerating Cosmology Without Dark Energy

The negative pressure accompanying gravitationally-induced particle creation can lead to a cold dark matter (CDM) dominated, accelerating Universe (Lima et al. 1996) without requiring the presence of dark energy or a cosmological constant. In a recent study Lima et al. (2008, LSS) demonstrated that particle creation driven cosmological models are capable of accounting for the SNIa observations of the recent transition from a decelerating to an accelerating Universe. Here we test the evolution of such models at high redshift using the constraint on z_eq, the redshift of the epoch of matter radiation equality, provided by the WMAP constraints on the early Integrated Sachs-Wolfe effect. Since the contribution of baryons and radiation was ignored in the work of LSS, we include them in our study of this class of models. The parameters of these more realistic models with continuous creation of CDM is tested and constrained at widely-separated epochs (z = z_eq and z = 0) in the evolution of the Universe. This comparison reveals a tension between the high redshift CMB constraint on z_eq and that which follows from the low redshift SNIa data, challenging the viability of this class of models.Comment: 12 pages, 8 figure

Limits on decaying dark energy density models from the CMB temperature-redshift relation

The nature of the dark energy is still a mystery and several models have been proposed to explain it. Here we consider a phenomenological model for dark energy decay into photons and particles as proposed by Lima (J. Lima, Phys. Rev. D 54, 2571 (1996)). He studied the thermodynamic aspects of decaying dark energy models in particular in the case of a continuous photon creation and/or disruption. Following his approach, we derive a temperature redshift relation for the CMB which depends on the effective equation of state $w_{eff}$ and on the "adiabatic index" $\gamma$. Comparing our relation with the data on the CMB temperature as a function of the redshift obtained from Sunyaev-Zel'dovich observations and at higher redshift from quasar absorption line spectra, we find $w_{eff}=-0.97 \pm 0.034$, adopting for the adiabatic index $\gamma=4/3$, in good agreement with current estimates and still compatible with $w_{eff}=-1$, implying that the dark energy content being constant in time.Comment: 8 pages, 1 figur

Unified dark energy models : a phenomenological approach

A phenomenological approach is proposed to the problem of universe accelerated expansion and of the dark energy nature. A general class of models is introduced whose energy density depends on the redshift $z$ in such a way that a smooth transition among the three main phases of the universe evolution (radiation era, matter domination, asymptotical de Sitter state) is naturally achieved. We use the estimated age of the universe, the Hubble diagram of Type Ia Supernovae and the angular size - redshift relation for compact and ultracompact radio structures to test whether the model is in agreement with astrophysical observation and to constrain its main parameters. Although phenomenologically motivated, the model may be straightforwardly interpreted as a two fluids scenario in which the quintessence is generated by a suitably chosen scalar field potential. On the other hand, the same model may also be read in the context of unified dark energy models or in the framework of modified Friedmann equation theories.Comment: 12 pages, 10 figures, accepted for publication on Physical Review

Effect of electromagnetic fields on the creation of scalar particles in a flat Robertson-Walker space-time

The influence of electromagnetic fields on the creation of scalar particles from vacuum in a flat Robertson-Walker space-time is studied. The Klein Gordon equation with varying electric field and constant magnetic one is solved. The Bogoliubov transformation method is applied to calculate the pair creation probability and the number density of created particles. It is shown that the electric field amplifies the creation of scalar particles while the magnetic field minimizes it.Comment: Important modifications, 20 pages, To appear in Eurpean Physical Journal C. arXiv admin note: text overlap with arXiv:1108.033

Thimet oligopeptidase (EC 3.4.24.15) key functions suggested by knockout mice phenotype characterization

Thimet oligopeptidase (THOP1) is thought to be involved in neuropeptide metabolism, antigen presentation, neurodegeneration, and cancer. Herein, the generation of THOP1 C57BL/6 knockout mice (THOP1(-/-)) is described showing that they are viable, have estrus cycle, fertility, and a number of puppies per litter similar to C57BL/6 wild type mice (WT). In specific brain regions, THOP1(-/-) exhibit altered mRNA expression of proteasome beta5, serotonin 5HT2a receptor and dopamine D2 receptor, but not of neurolysin (NLN). Peptidomic analysis identifies differences in intracellular peptide ratios between THOP1(-/-) and WT mice, which may affect normal cellular functioning. In an experimental model of multiple sclerosis THOP1(-/-) mice present worse clinical behavior scores compared to WT mice, corroborating its possible involvement in neurodegenerative diseases. THOP1(-/-) mice also exhibit better survival and improved behavior in a sepsis model, but also a greater peripheral pain sensitivity measured in the hot plate test after bradykinin administration in the paw. THOP1(-/-) mice show depressive-like behavior, as well as attention and memory retention deficits. Altogether, these results reveal a role of THOP1 on specific behaviors, immune-stimulated neurodegeneration, and infection-induced inflammation