The present and future offshore wind resource in the Southwestern African region

In the last decades, offshore wind harvesting has increased enormously, and is seen as a renewable energy resource with great potential in many regions of the world. Therefore, it is crucial to understand how this resource will evolve in a warming climate. In the present study, offshore wind resource in the Southwestern African region is analysed for the present and future climates. A ROM (REMO-OASIS-MPIOM) climate simulation in uncoupled and coupled atmosphere–ocean mode, at 25 km horizontal resolution, and a multi-model ensemble built with a set of regional climate models from the CORDEX-Africa experiment at 0.44° resolution were used. The projected changes of the offshore wind energy density throughout the twenty-first century are examined following the RCP4.5 and RCP8.5 greenhouse gas emissions scenarios. Characterised by strong coastal-parallel winds, the Southwestern African offshore region shows high values of wind energy density at 100 m, up to 1500 Wm⁻² near the coast, particularly offshore Namibia and west South Africa. Conversely, along Angola’s coast the available offshore wind energy density is lower. Throughout the twenty-first century, for the weaker climate mitigation scenario (RCP8.5), an increase of the offshore wind resource is projected to occur along Namibia and South African western coasts, more pronounced at the end of the century (+ 24%), while a decrease is projected along Angola’s coasts, reaching a negative anomaly of about − 32%. Smaller changes but with the same pattern are projected for the stronger climate mitigation scenario (RCP4.5). The future deployment of offshore floating hub turbines placed at higher heights may allow higher production of energy in this region. Along offshore Namibia and west South Africa, the wind energy density at 250 m showed differences that range between 30 and 50% relative to wind energy density at 100 m

Purification and characterization of a collagenase from Penicillium sp. UCP 1286 by polyethylene glycol-phosphate aqueous two-phase system

Collagenases are proteolytic enzymes capable of degrading both native and denatured collagen, reported to be applied in industrial, medical and biotechnological sectors. Liquid-liquid extraction using aqueous two-phase system (ATPS) is one of the most promising bioseparation techniques, which can substitute difficult solid-liquid separation processes, offering many advantages over conventional methods including low-processing time, low-cost material and low-energy consumption. The collagenase produced by Penicillium sp. UCP 1286 showed a stronger affinity for the bottom salt-rich phase, where the highest levels of collagenolytic activity were observed at the center point runs, using 15.0% (w/w) PEG 3350 g/mol and 12.5% (w/w) phosphate salt at pH 7.0 and concentration. The enzyme was characterized by thermal stability, pH tolerance and effect of inhibitors, showing optimal collagenolytic activity at 37 °C and pH 9.0 and proved to be a serine protease. ATPS showed high efficiency in the collagenase purification, confirmed by a single band in SDS/PAGE, and can in fact be applied as a quick and inexpensive alternative method.This work was supported by the National Council of Technological and Scientific Development (CNPq) and Coordination for the Improvement of Higher Education Personnel (CAPES). Sara Silvério also acknowledges her post-doc grant (SFRH/BPD/88584/2012) from FCT (SFRH/BPD/88584/2012) (Portuguese Science and Technology Foundation), Portugal

The partial inhibition of hypothalamic IRX3 exacerbates obesity

The Iroquois homeobox 3 (Irx3) gene has been identified as a functional long-range target of obesity-associated variants within the fat mass and obesity-associated protein (FTO) gene. It is highly expressed in the hypothalamus, and both whole-body knockout and hypothalamic restricted abrogation of its expression results in a lean phenotype, which is mostly explained by the resulting increased energy expenditure in the brown adipose tissue. Because of its potential implication in the pathogenesis of obesity, we evaluated the hypothalamic cell distribution of Irx3 and the outcomes of inhibiting its expression in a rodent model of diet-induced obesity. Methods: Bioinformatics tools were used to evaluate the correlations between hypothalamic Irx3 and neurotransmitters, markers of thermogenesis and obesity related phenotypes. Droplet-sequencing analysis in >20,000 hypothalamic cells was used to explore the types of hypothalamic cells expressing Irx3. Lentivirus was used to inhibit hypothalamic Irx3 and the resulting phenotype was studied. Findings: IRX3 is expressed predominantly in POMC neurons. Its expression is inhibited during prolonged fasting, as well as when mice are fed a high-fat diet. The partial inhibition of hypothalamic Irx3 using a lentivirus resulted in increased diet-induced body mass gain and adiposity due to increased caloric intake and reduced energy expenditure. Interpretation: Contrary to the results obtained when lean mice are submitted to complete inhibition of Irx3, partial inhibition of hypothalamic Irx3 in obese mice causes an exacerbation of the obese phenotype. These data suggest that at least some of the Irx3 functions in the hypothalamus are regulated according to a hormetic pattern, and modulation of its expression can be a novel approach to modifying the body's energy-handling regulation.39448460FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP2013/07607-8; 2017/02983-

The North African coastal low level wind jet: a high resolution view

The North African coastal low-level jet (NACLLJ) lies over the cold Canary current and is synoptically linked to the Azores Anticyclone and to the continental thermal low over the Sahara Desert. Although being one of the most persistent and horizontally extended coastal wind jets, this is the first high resolution modelling effort to investigate the NACLLJ climate. The current study uses a ROM atmospheric hindcast simulation with ~ 25 km resolution, for the period 1980–2014. Additionally, the underlying surface wind features are also scrutinized using the CORDEX-Africa runs. These runs allow the building of a multi-model ensemble for the coastal surface flow. The ROM and the CORDEX-Africa simulations are extensively evaluated showing a good ability to represent the surface winds. The NACLLJ shows a strong seasonal cycle, but, unlike most coastal wind jets, e.g. the California one, it is significantly present all year round, with frequencies of occurrence above 20%. In spring and autumn, the maxima frequencies are around 50%, and reach values above 60% in summer. The location of maximum frequency of occurrence migrates meridionally from season to season, being in winter and spring upwind of Cap-Vert, and in summer and autumn offshore the Western Sahara. Analogously, the lowest jet wind speeds occur in winter, when the median is below 15 m/s. In summer, the jet wind speed median values are ~ 20 m/s and the maxima are above 30 m/s. The jet occurs at heights ~ 360 m. A momentum balance is pursued disclosing that the regional flow is almost geostrophic, dominated by the pressure gradient and Coriolis force. Over the jet areas the ageostrophy is responsible for the jet acceleration

Assessing the climate change impact on the North African offshore surface wind and coastal low-level jet using coupled and uncoupled regional climate simulations

The North African Coastal Low-Level Jet (NACLLJ) is a semi-permanent feature offshore the north western African coast, linked to the cold nearshore upwelling of the Canary Eastern Boundary Current system. Its main synoptic drivers are the Azores Anticyclone over the ocean and the inland Sahara thermal low. The coastal jet events occur in one of the world’s most productive fisheries region, thus the evaluation of the effects of global warming in its properties is imperative. This study proposes an analysis of the annual and intra-annual attributes of the NACLLJ for two time periods 1976–2005 (historical) and 2070–2199 (future), resorting to coupled and uncoupled atmosphere–ocean simulations with the ROM model, as well as near surface offshore wind speed from the CORDEX-Africa ensemble. The future simulations follow the RCP8.5 greenhouse gas emissions scenario. Overall, the ROM coupled simulation presents the best performance in reproducing the present-climate near surface wind speed, offshore northwest Africa, compared to the remaining RCM simulations. The higher SST resolution in the coupled simulations favours much localised colder upwelling strips near the coast and consequently stronger jets. In future climate, a small increase in the surface wind speed is projected, mainly linked to the regions of coastal jet presence. The NACLLJ is projected to be more frequent and intense, encompassing larger areas. An increase of the jet seasonal frequencies of occurrence is projected for all seasons, which is larger from spring to autumn (up to 15, 16 and 22% more frequent, respectively). However, in some offshore areas the winter NACLLJ persistency is likely to double, relatively to present-climate. Higher inter-annual variability is also projected for the future NACLLJ seasonal frequencies. The strengthening of the coastal jet speeds is also significant, between 5 and 12% in all seasons. Additionally, the jet’s diurnal cycle shows an increase in jet occurrence across the day, particularly in the mid and late afternoon

Aminodi(hetero)arylamines in the thieno[3,2-b]pyridine series: synthesis, effects in human tumor cells growth, cell cycle analysis, apoptosis and evaluation of toxicity using non-tumor cells

Three aminodi(hetero)arylamines were prepared via a palladium-catalyzed C-N Buchwald-Hartwig coupling of methyl 3-aminothieno[3,2-b]pyridine-2-carboxylate with different bromonitrobenzenes, followed by reduction of the nitro groups of the coupling products to the corresponding amino compounds. The aminodi(hetero)arylamines thus obtained were evaluated for their growth inhibitory effect on four human tumor cell lines MCF-7 (breast adenocarcinoma), A375-C5 (melanoma), NCI-H460 (non-small cell lung cancer) and HepG2 (hepatocellular carcinoma). The toxicity to non-tumor cells was also evaluated using a porcine liver primary cell culture (PLP1), established by us. The aminodi(hetero)arylamine with the NH2 group in the ortho position and an OMe group in the para position to the NH of the di(hetero)arylamine, is the most promising compound giving the lowest GI50 values (1.30–1.63 μM) in all the tested human tumor cell lines, presenting no toxicity to PLP1 at those concentrations. The effect of this compound on the cell cycle and induction of apoptosis was analyzed in the NCI-H460 cell line. It was observed that it altered the cell cycle profile causing a decrease in the percentage of cells in the G0/G1 phase and an increase of the apoptosis levels.Foundation for the Science and Technology (FCT–Portugal) for financial support through the NMR Portuguese network (Bruker 400 Avance III-Univ Minho). FCT and FEDER (European Fund for Regional Development) for financial support through the research centers PEst-C/QUI/UI686/2011and PEst-OE/AGR/UI0690/2011, the research project PTDC/QUI-QUI/111060/2009 and the post-Doctoralgrants attributed to R.C.C. and R.T.L. (SFRH/BPD/68344/2010 and SFRH/BPD/68787/2010, respectively). IPATIMUP is an Associate Laboratory of the Portuguese Ministry of Science, Technology and Higher Education and is partially supported by FCT

Combination of Pneumococcal Surface Protein A (PspA) with Whole Cell Pertussis Vaccine Increases Protection Against Pneumococcal Challenge in Mice

Streptococcus pneumoniae is the leading cause of respiratory acute infections around the world. In Latin America, approximately 20,000 children under 5 years of age die of pneumococcal diseases annually. Pneumococcal surface protein A (PspA) is among the best-characterized pneumococcal antigens that confer protection in animal models of pneumococcal infections and, as such, is a good alternative for the currently available conjugated vaccines. Efficient immune responses directed to PspA in animal models have already been described. Nevertheless, few low cost adjuvants for a subunit pneumococcal vaccine have been proposed to date. Here, we have tested the adjuvant properties of the whole cell Bordetella pertussis vaccine (wP) that is currently part of the DTP (diphtheria-tetanus-pertussis) vaccine administrated to children in several countries, as an adjuvant to PspA. Nasal immunization of BALB/c mice with a combination of PspA5 and wP or wPlow – a new generation vaccine that contains low levels of B. pertussis LPS – conferred protection against a respiratory lethal challenge with S. pneumoniae. Both PspA5-wP and PspA5-wPlow vaccines induced high levels of systemic and mucosal antibodies against PspA5, with similar profile, indicating no essential requirement for B. pertussis LPS in the adjuvant properties of wP. Accordingly, nasal immunization of C3H/HeJ mice with PspA5-wP conferred protection against the pneumococcal challenge, thus ruling out a role for TLR4 responses in the adjuvant activity and the protection mechanisms triggered by the vaccines. The high levels of anti-PspA5 antibodies correlated with increased cross-reactivity against PspAs from different clades and also reflected in cross-protection. In addition, passive immunization experiments indicated that antibodies played an important role in protection in this model. Finally, subcutaneous immunization with a combination of PspA5 with DTPlow protected mice against challenge with two different pneumococcal strains, opening the possibility for the development of a combined infant vaccine composed of DTP and PspA