Influências do fumo sobre a espessura do epitélio oral da gengiva marginal

Smoking patients show reduction of inflammatory clinical signs that might be associated with local vasoconstriction and an increased gingival epithelial thickness. The purpose of this work was to evaluate the thickness of the marginal gingival oral epithelium in smokers and non-smokers, with clinically healthy gingivae or with gingivitis. Twenty biopsies were obtained from four different groups. Group I: non-smokers with clinically healthy gingivae (n = 5). Group II: non-smokers with gingivitis (n = 5). Group III: smokers with clinically healthy gingivae (n = 5). Group IV: smokers with gingivitis (n = 5). These biopsies were histologically processed, serially sectioned at 5 mm, stained with H. E., and examined by image analysis software (KS400), which was used to perform the morphometric evaluation and the quantification of the major epithelial thickness, the epithelial base thickness and the external and internal epithelial perimeters. Differences between the four groups were analyzed using ANOVA test and Tukey's test. The criteria for statistical significance were accepted at the probability level p < 0.05. A greater epithelial thickness was observed in smokers independent of the gingival health situation.Pacientes fumantes freqüentemente apresentam redução dos sinais clínicos inflamatórios da gengivite, associada, em grande parte, a vasoconstrição local e aumento da espessura epitelial. O objetivo deste trabalho foi avaliar a espessura do epitélio oral da gengiva marginal de pacientes fumantes e não fumantes, nos estados de saúde gengival e gengivite. Foram obtidos vinte fragmentos de tecido gengival de quatro grupos de pacientes. Grupo I: não fumantes com saúde gengival (n = 5). Grupo II: não fumantes com gengivite (n = 5). Grupo III: fumantes com saúde gengival (n = 5). Grupo IV: fumantes com gengivite (n = 5). As biópsias receberam processamento histológico de rotina, e os cortes semi-seriados com 5 mm de espessura foram corados com H. E. Com auxílio do sistema de imagens KS400 foram quantificadas a espessura epitelial maior e a espessura da base epitelial e determinados os valores dos perímetros epiteliais externo e interno. Os dados foram avaliados pelo ANOVA e pelo teste de Tukey, considerando significativo o valor de a = 0,05. Os resultados mostraram valores da espessura da base epitelial maior (p < 0,05) nos pacientes fumantes, independentemente do estado de saúde gengival

Avaliação clinica do tratamento de lesões de bifurcação grau II

Tese (Livre-docencia) - Universidade Estadual de Campinas, Faculdade de Odontologia de PiracicabaResumo: Este estudo avaliou o preenchimento ósseo de defeitos de bifurcação grau II tratados pela técnica de regeneração tecidual guiada com membranas de politetrafluoroetileno nas formas expandida em associação com enxerto de mistura óssea, ou sólida. Foram considerados os parâmetros clínicos de índice de Placa (IPI), Gengival (IG), e de Sangramento à Sondagem (ISS), e os parâmetros biométricos de Nível da Margem Gengival (NMG), Nível Clínico de Inserção (NCI), Profundidade de Sondagem (PS), Nível de Inserção Horizontal (NIH), Nível Ósseo Vertical (NOV) e Horizontal (NOH) e Nível da Crista Óssea Alveolar (NCOA). As medidas foram obtidas no baseline e comparadas com as da reentrada cirúrgica, realizada 6 meses após a colocação das barreiras. Os procedimentos foram realizados em 28 indivíduos adultos de ambos os sexos, com idade variando entre 22 e 68 anos, nos quais foram selecionados primeiros e segundos molares inferiores e superiores cuja evolução da doença periodontal provocou a lesão de bifurcação. Todas as lesões foram tratadas sob anestesia local e retalhos mucoperiostais que possibilitaram a instrumentação manual, ultra-sônica ou rotatória, e o condicionamento com solução de tetraciclina na concentração de 50mg/ml. Os molares superiores receberam membranas de politetrafluoroetileno sólido e os molares inferiores membranas de politetrafluoroetileno sólido ou expandido em associação com enxerto de mistura óssea, segundo o protocolo experimental de cada grupo. Após a seleção, recorte, adaptação e sutura das membranas, os retalhos foram recolocados e suturados de maneira a recobrir totalmente as barrreiras. Os voluntários foram mantidos sob orientação para o efetivo controle de placa bacteriana com bochechos e aplicação tópica diárias com digluconato de clorexidina 0,12% até a remoção das suturas dos retalhos, e com bochechos diários com digluconato de clorexidina à 0,12% até completarem o período de seis semanas quando as barreiras foram removidas. Os resultados mostraram redução para os parâmetros clínicos (PI, IG e ISS (p0,05) entre as três modalidades de tratamento. Houve ganho clínico no NIH (p0,05) entre as três modalidades de tratamento. Os resultados deste estudo indicam que há preenchimento ósseo das lesões de bifurcação grau II em molares superiores e inferiores tratadas segundo os princípios da técnica de regeneração tecidual guiada em associação ou não com enxerto de mistura ósseaAbstract: Bone fill in class II furcation defects in human teeth was studied following guided tissue regeneration (GTR) procedures using both expanded polytetrafluoroethylene (e-PTFE) plus bone blending and polytetrafluoroethilene (PTFE) membranes alone. The following clinical assessments were taken: Plaque Index (Pll), Gingival Index (Gl), Blending on Probing (BP), gingival margin level (GML), clinical attachment level (CAL), probing depth (PD), horizontal attachment level (HAL), and surgical assessments like vertical bone level (VBL), horizontal bone level (HBL) and alveolar bone crest level (ABCL). The measurements were taken at baseline and re-entry surgery after 6 months. The procedures were performed in 28 adult patients, 22 to 68 years old, whose first and second maxillary and mandibular molars were involved by adult periodontitis and degree II furcation defects. Mucoperiosteal flaps were raised and the defects were treated with hand, ultrasonic and rotatory instruments. Tetracycline hydrochloride (50mg/ml) was applied on the root surface for 5 minutes. The maxillary molars received the PTFE membrane, and the mandibular molars received the PTFE membrane alone, or e-PTFE membrane plus bone blending. Matress suture kept the membranes in place. Oral hygiene was accomplished by soft tooth brushing and rinsing the surgical area with 0.12% chlorexidine gluconate twice a day for seven days after surgery when the sutures were removed - the patients were maintained with the same oral hygienic regime for six weeks, when the PTFE/e-PTFE material was removed. The finding showed better results for Pll, Gl and BP, and there was statistical difference (p0,05) between baseline and re-entry procedures for all the treatment modalities. There was clinical difference in HAL (p0,05) among all the treatment modalities. Within the limits of this study, it was concluded that there is significant bone fill in degree II human furcation defects in maxillary and mandibular molars in humans treated by guided tissue regeneration with or without bone blendingLivre DocênciaPeriodontiaLivre Docente em Clinica Odontológic

Quantificação da pressão e avaliação histobacteriologica da raspagem dental e do alisamento radicular "in vitro"

Orientadores: Lourenço Bozzo , Homero Habel RodriguesDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de PiracicabaResumo: Não informadoAbstract: Not informed.MestradoPatologiaMestre em Ciência

[characteristics Of Implant Surface And Implant-abutment Gap In Two- And One-stage Systems].

The aim of this study was to evaluate under scanning electron microscopy the characteristics of the implant surface and the gap between the components of two- and one-stage systems. Three two-stage implants coated with RBM and three one-stage implants coated with TPS were selected. In the two-stage implants, the esthetic-cone abutments were adapted and screw tightened with 20 N/cm . In the one stage implants, solid abutments were adapted and torque tightened mechanical frictional with 30 N/cm. The specimens were mounted on stubs and analyzed under scanning electron microscopy. The gap in each implant was measured in four different points and repeated three times. The paired Student-t test was applied to detect the difference in the gap extension. The results showed that no significant differences (P > 0.05) were found regarding the gap extension between two- and one-stage systems and that the treatments produced different surface roughness.11107-1

Características da superfície e da fenda implante-intermediário em sistemas de dois e um estágios

The aim of this study was to evaluate under scanning electron microscopy the characteristics of the implant surface and the gap between the components of two- and one-stage systems. Three two-stage implants coated with RBM and three one-stage implants coated with TPS were selected. In the two-stage implants, the esthetic-cone abutments were adapted and screw tightened with 20 N/cm . In the one stage implants, solid abutments were adapted and torque tightened mechanical frictional with 30 N/cm. The specimens were mounted on stubs and analyzed under scanning electron microscopy. The gap in each implant was measured in four different points and repeated three times. The paired Student-t test was applied to detect the difference in the gap extension. The results showed that no significant differences (P >; 0.05) were found regarding the gap extension between two- and one-stage systems and that the treatments produced different surface roughness.O objetivo deste estudo foi avaliar em microscopia eletrônica de varredura as características da superfície e da fenda existente entre os componentes de implantes de dois e um estágios. Foram selecionados 3 implantes de dois estágios revestidos com fosfato de cálcio cerâmico (RBM) e 3 implantes de um estágio revestidos com plasma de titânio (TPS). Nos implantes de dois estágios, os intermediários tipo esthetic-cone foram adaptados ao hexágono externo e travados com parafuso com torque definitivo de 20 N/cm. Nos implantes de um estágio foram utilizados intermediários sólidos que foram adaptados e fixados por travamento friccional com torque definitivo de 30 N/cm. Os espécimes foram montados em stubs e analisados em microscopia eletrônica de varredura. A fenda foi medida em quatro pontos com três repetições em cada implante. Os valores obtidos foram avaliados pelo teste t pareado de Student. Os resultados mostraram que não houve diferença estatística significativa (P >; 0,05) na extensão da fenda entre os sistemas de um e dois estágios e que os tratamentos proporcionaram diferentes características de superfície

Comparação entre fibroblastos gengivais e do ligamento periodontal de um mesmo indivíduo

The objective of this study was to compare fibroblasts from the periodontal ligament (PLF) and gingival fibroblasts (GF) as to morphology, proliferation rate and protein synthesis. PLF and GF were explanted from tissues of the same patient. To characterize and compare the morphology of cells, PLF and GF were plated and analyzed under phase-contrast and optical microscopies. Proliferation rates were determined by means of automated counts carried out in days 1, 4, 7, 15 and 21, and also by means of the bromodeoxyuridine labelling index (BrdU). Total protein content was analyzed by means of electrophoresis in 10% polyacrylamide gel and zimography containing gelatin as substrate. PLF were bigger and more elongated than GF in subconfluence and confluence conditions. The proliferative rate of PLF was higher than that of GF at 1, 4, and 7 days (p < 0.05). At 15 and 21 days, there was no statistically significant difference as to the number of cells. PLF presented a significantly greater proliferative potential, in relation to GF (p < 0.05). The synthesis of protein in a period of 24 hours was similar for both PLF and GF. Our results demonstrated that PLF and GF are different as to morphology and proliferative capacity, however, they do not differ as to protein synthesis.O objetivo deste estudo foi comparar as características morfológicas, o potencial proliferativo e a produção protéica de fibroblastos do ligamento periodontal (FLP) e de fibroblastos gengivais (FG). Os fibroblastos foram cultivados pela técnica do explante a partir de fragmentos da gengiva e do ligamento periodontal de um mesmo indivíduo. As células foram isoladas e plaqueadas para análise por microscopia de contraste de fase e microscopia óptica. O índice de proliferação celular foi determinado por contagem automática de células e pelo ensaio de incorporação de bromodioxiuridina (BrdU). A produção de proteína total foi verificada por eletroforese em gel de poliacrilamida e o perfil enzimático por análise zimográfica. Os FLP são maiores e mais alongados que os FG em condições de subconfluência e confluência celular. Os FLP demonstraram um potencial proliferativo significantemente maior que os FG. Os perfis protéico e enzimático foram similares entre os FLP e FG. Os resultados demonstram que os FLP e FG são diferentes na morfologia e na capacidade proliferativa, porém são semelhantes na produção protéica

Comparison between gingival and periodontal ligament fibroblasts from the same subject

FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOThe objective of this study was to compare fibroblasts from the periodontal ligament (PLF) and gingival fibroblasts (GF) as to morphology, proliferation rate and protein synthesis. PLF and GF were explanted from tissues of the same patient. To characterize and compare the morphology of cells, PLF and GF were plated and analyzed under phase-contrast and optical microscopies. Proliferation rates were determined by means of automated counts carried out in days 1, 4, 7, 15 and 21, and also by means of the bromodeoxyuridine labelling index (BrdU). Total protein content was analyzed by means of electrophoresis in 10% polyacrylamide gel and zimography containing gelatin as substrate. PLF were bigger and more elongated than GF in subconfluence and confluence conditions. The proliferative rate of PLF was higher than that of GF at 1, 4, and 7 days (p < 0.05). At 15 and 21 days, there was no statistically significant difference as to the number of cells. PLF presented a significantly greater proliferative potential, in relation to GF (p < 0.05). The synthesis of protein in a period of 24 hours was similar for both PLF and GF. Our results demonstrated that PLF and GF are different as to morphology and proliferative capacity, however, they do not differ as to protein synthesis.O objetivo deste estudo foi comparar as características morfológicas, o potencial proliferativo e a produção protéica de fibroblastos do ligamento periodontal (FLP) e de fibroblastos gengivais (FG). Os fibroblastos foram cultivados pela técnica do explante a partir de fragmentos da gengiva e do ligamento periodontal de um mesmo indivíduo. As células foram isoladas e plaqueadas para análise por microscopia de contraste de fase e microscopia óptica. O índice de proliferação celular foi determinado por contagem automática de células e pelo ensaio de incorporação de bromodioxiuridina (BrdU). A produção de proteína total foi verificada por eletroforese em gel de poliacrilamida e o perfil enzimático por análise zimográfica. Os FLP são maiores e mais alongados que os FG em condições de subconfluência e confluência celular. Os FLP demonstraram um potencial proliferativo significantemente maior que os FG. Os perfis protéico e enzimático foram similares entre os FLP e FG. Os resultados demonstram que os FLP e FG são diferentes na morfologia e na capacidade proliferativa, porém são semelhantes na produção protéica164319325FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO1999/10767-8; 1999/04921-4The objective of this study was to compare fibroblasts from the periodontal ligament (PLF) and gingival fibroblasts (GF) as to morphology, proliferation rate and protein synthesis. PLF and GF were explanted from tissues of the same patient. To characterize and compare the morphology of cells, PLF and GF were plated and analyzed under phase-contrast and optical microscopies. Proliferation rates were determined by means of automated counts carried out in days 1, 4, 7, 15 and 21, and also by means of the bromodeoxyuridine labelling index (BrdU). Total protein content was analyzed by means of electrophoresis in 10% polyacrylamide gel and zimography containing gelatin as substrate. PLF were bigger and more elongated than GF in subconfluence and confluence conditions. The proliferative rate of PLF was higher than that of GF at 1, 4, and 7 days (p < 0.05). At 15 and 21 days, there was no statistically significant difference as to the number of cells. PLF presented a significantly greater proliferative potential, in relation to GF (p < 0.05). The synthesis of protein in a period of 24 hours was similar for both PLF and GF. Our results demonstrated that PLF and GF are different as to morphology and proliferative capacity, however, they do not differ as to protein synthesi

Smoking influences on the thickness of marginal gingival epithelium

Smoking patients show reduction of inflammatory clinical signs that might be associated with local vasoconstriction and an increased gingival epithelial thickness. The purpose of this work was to evaluate the thickness of the marginal gingival oral epithelium in smokers and non-smokers, with clinically healthy gingivae or with gingivitis. Twenty biopsies were obtained from four different groups. Group I: non-smokers with clinically healthy gingivae (n = 5). Group II: non-smokers with gingivitis (n = 5). Group III: smokers with clinically healthy gingivae (n = 5). Group IV: smokers with gingivitis (n = 5). These biopsies were histologically processed, serially sectioned at 5 mm, stained with H. E., and examined by image analysis software (KS400), which was used to perform the morphometric evaluation and the quantification of the major epithelial thickness, the epithelial base thickness and the external and internal epithelial perimeters. Differences between the four groups were analyzed using ANOVA test and Tukey's test. The criteria for statistical significance were accepted at the probability level p < 0.05. A greater epithelial thickness was observed in smokers independent of the gingival health situation