Phytochemical screening and anti-cancer effect of Clinacanthus nutans extract towards cervical cancer cell line, HeLa

Nowadays many researches are focusing on the discovery of new therapeutic substance of natural origin for the treatment of cancer based on its cytoxicity to human cells. Clinacanthus nutans (C.nutans) or locally known as Belalai Gajah in Malaysia have been used in Thailand as a folk medicine for the Herpes virus infection. Recently C.nutans had become popular folk medicine in the treatment of cancer around Malaysia. In the present study, we aim to examine anti-cancer activities of C.nutans extracts for the treatment of cancer in vitro. Aqueous and methanol crude extracts were extracted from the leaves of C.nutans and phytochemical screening was performed to study the plant secondary metabolites. The cytotoxic activity of both crude extracts were investigated in vitro against human cervical cancer cell lines, HeLa by using MTT assay. The mode of cell death induced by the crude extracts of C.nutans was examined by Hoechst 33258 stain. The secondary metabolism constituents in aqueous crude extract are terpenoids and flavonoids, whereas methanol crude extract contains terpemoids, alkaloids and flavonoids. Our results showed that C.nutans in aqueous extract exerted a significant antiproliferative effect on HeLa cells (ICso=13±O.82 pg/mL) and but no ICsowas detected by methanol extract on HeLa cells. No significant activities (IC50 = not detected) were present in vero cells treated with both aqueous and methanol crude extract. Whereas HeLa and Vero cells treated with control drug, tamoxifen shows a significant cytotoxicity effect with IC50 values of 3.8±0.19 pg/mL and 2.2±0.029 pg/mL respectively. Hoechst 33258 stain showed that aqueous extract of C.nutans induce cell death on HeLa cells via apoptosis. The aqueous extract of C.nutans exert the anti-cancer activity against cultured human cervical cancer cell lines with cytoselectivity property and induces cell death by apoptosis pathway , suggesting a promising alternate therapeutic substance for cancer prevention and treatmen

A Comparative Study of Deep Learning Model and Simple Prediction Charts in Construction Noise Prediction

Construction noise monitoring is crucial to assess the impacts of construction noise on the workers and surroundings. However, the existing noise prediction methods are time-consuming in which required laborious work for the computation of noise levels. This study aims to assess the accuracy and reliability of deep learning model (DL) that adopted stochastic modelling and artificial neural network (ANN) in construction noise prediction. The artificial neural network was trained with the output of stochastic modelling. The outcome of noise level prediction using simple prediction chart (SPC) and DL model was discussed and compared to 3 case studies. The case studies were conducted at construction sites located in Semenyih, Selangor, Malaysia. The results of DL model showed high accuracy of predicted noise levels along with an absolute difference of less than 2.3 dBA. Besides, the predicted noise levels are reliable as the R-squared value is higher than 0.992. On that account, DL model is proved to be reliable and accurate in noise level prediction and it has the potential to be utilized as a managerial tool to monitor construction noise more effectively

Effect of Ceramic Dust as Partial Replacement of Cement on Lightweight Foamed Concrete

Disposal of waste into the landfill causes a severe impact on the environment. One of the waste products is ceramic waste. Ceramic waste has some excellent properties in its durability, hardness, and highly resistant to biological, chemical, and physical degradation forces. These excellent properties of the ceramic waste may make it suitable to be used in concrete. This study investigates the effect on the compressive strength of lightweight foamed concrete with different percentage of ceramic dust replacement level towards the cement and three different levels of water-cement ratio. 0%, 5%, 15%, and 25% of replacement level with 0.52, 0.56, and 0.60 water-cement ratios respectively for each replacement level was used as the parameter to investigate the fresh properties, and strength performance of lightweight foamed concrete. The stability and consistency of every mix are studied as well. From this study, it was observed that the incorporation of ceramic waste dust partially replaced the cement did not affect on the fresh properties of the foamed concrete. However, the compressive strength of foamed concrete affected by ceramic waste dust partially replaced the cement

EDI in Singapore: emerging issues with sexual and gender minorities and people living with HIV

Diversity management had always been at the forefront of Singapore's social and economic policies. Over the last half a century, a slew of legislation, social and economic policies aimed at maintaining harmony and ensuring economic progress have successfully put Singapore on the world map as a global trading hub. Owing to Singapore's heritage as a migrant nation, much of the diversity management efforts in the past had focused on bases of diversity such as age, race, gender, religion. However, in recent years, there was much public discourse on the inclusion of gender and sexual minorities and people living with HIV, pushing for a greater need to address issues that have long been considered sensitive. This chapter spotlights the two issues against a backdrop of how diversity is managed in the Singapore context, and discusses two frameworks that may help shed more light on Singapore's approach to diversity management.acceptedVersio

Experimental Study on the Flexural Behavior of Reinforced Polystyrene Blocks in Concrete Beams

A new type of lightweight beam system was recently proposed by embedding polystyrene in beams to improve structural efficiency. This removes the non-performing concrete in the neutral axis and tension region to provide a comparable strength as a solid beam. There are, however, limited studies conducted to investigate the structural behavior of such beams. Therefore, this research presents an experimental investigation to assess the effect of polystyrene shapes in the beams. This involved testing a solid beam and five lightweight beams under flexural load using a four-point load test. The inclusion of polystyrene was estimated to have reduced the self-weight of beams by 8.6% to 11.8% when compared with the solid beam. The results also showed the ellipse polystyrene with a width of 70 mm and height of 50 mm produced the highest effective strength to weight ratio (sw) of 1.12 and performed 12% better than the solid beam. Moreover, the lightweight beams have more weight reduced than the strength, and those with ellipse polystyrene were found to have performed better than circular ones based on first crack load, ultimate load, and effective strength to weight ratio (sw). The beams with ellipse polystyrene allowed better stress distribution and this gave them a higher strength than sphere shape. For industry application, the polystyrene content is recommended to be greater than 10% while the effective strength to weight ratio (sw) of the beam is greater than 1. The successful reduction of the weight without affecting the structural performance has the ability to help in reducing construction costs

Methane Migration and Its Influence on Sulfate Reduction in the Good Weather Ridge Region, South China Sea Continental Margin Sediments

Bacteria sulfate reduction is a major pathway for organic carbon oxidation in marine sediments. Upward diffusion of methane from gas hydrate deep in the sedimentary strata might be another important source of carbon for sulfate reducing bacteria and subsequently induce higher rates of sulfate reduction in sediments. Since abundant gas may migrate upward to the surface as a result of tectonic activity occurring in the accretionary wedge, this study investigates the effect of methane migration on the sulfate reduction process in continental margin sediments offshore southwestern Taiwan. Piston and gravity core samples were taken in order to evaluate vertical and spatial variations of sulfate and methane. Pore water sulfate, sulfide, methane, sediment pyrite, and organic carbon were extracted and analyzed

A Novel Respiratory Rate Estimation Algorithm from Photoplethysmogram Using Deep Learning Model

Respiratory rate (RR) is a critical vital sign that can provide valuable insights into various medical conditions, including pneumonia. Unfortunately, manual RR counting is often unreliable and discontinuous. Current RR estimation algorithms either lack the necessary accuracy or demand extensive window sizes. In response to these challenges, this study introduces a novel method for continuously estimating RR from photoplethysmogram (PPG) with a reduced window size and lower processing requirements. To evaluate and compare classical and deep learning algorithms, this study leverages the BIDMC and CapnoBase datasets, employing the Respiratory Rate Estimation (RRest) toolbox. The optimal classical techniques combination on the BIDMC datasets achieves a mean absolute error (MAE) of 1.9 breaths/min. Additionally, the developed neural network model utilises convolutional and long short-term memory layers to estimate RR effectively. The best-performing model, with a 50% train–test split and a window size of 7 s, achieves an MAE of 2 breaths/min. Furthermore, compared to other deep learning algorithms with window sizes of 16, 32, and 64 s, this study’s model demonstrates superior performance with a smaller window size. The study suggests that further research into more precise signal processing techniques may enhance RR estimation from PPG signals

Patients with more comorbidities have better detection of chronic conditions, but poorer management and control:findings from six middle-income countries

Background The burden of non-communicable diseases (NCDs) is rising rapidly in middle-income countries (MICs), where NCDs are often undiagnosed, untreated and uncontrolled. How comorbidity impacts diagnosis, treatment, and control of NCDs is an emerging area of research inquiry and have important clinical implications as highlighted in the recent National Institute for Health and Care Excellence guidelines for treating patients suffering from multiple NCDs. This is the first study to examine the association between increasing numbers of comorbidities with being undiagnosed, untreated, and uncontrolled for NCDs, in 6 large MICs. Methods Cross-sectional analysis of the World Health Organisation Study of Global Ageing and Adult Health (WHO SAGE) Wave 1 (2007–10), which consisted of adults aged ≥18 years from 6 populous MICs, including China, Ghana, India, Mexico, Russia and South Africa (overall n = 41, 557). Results A higher number of comorbidities was associated with better odds of diagnosis for hypertension, angina, and arthritis, and higher odds of having treatment for hypertension and angina. However, more comorbidities were associated with increased odds of uncontrolled hypertension, angina, arthritis, and asthma. Comorbidity with concordant conditions was associated with improved diagnosis and treatment of hypertension and angina. Conclusion Patients with more comorbidities have better diagnosis of chronic conditions, but this does not translate into better management and control of these conditions. Patients with multiple NCDs are high users of health services and are at an increased risk of adverse health outcomes. Hence, improving their access to care is a priority for healthcare systems

Roles and recommendations from primary care physicians towards managing low-risk breast cancer survivors in a shared-care model with specialists in Singapore-a qualitative study.

BackgroundBreast cancer is prevalent and has high cure rates. The resultant increase in numbers of breast cancer survivors (BCS) may overwhelm the current oncology workforce in years to come. We postulate that primary care physicians (PCPs) could play an expanded role in comanaging survivors, provided they are given the appropriate tools and training to do so.ObjectiveTo explore the perspectives of PCPs towards managing BCS in a community-based shared-care programme with oncologists.MethodsEleven focus groups and six in-depth interviews were conducted with seventy PCPs recruited by purposive sampling. All sessions were audio-recorded, transcribed verbatim and coded by three independent investigators. Thematic data analysis was performed and the coding process facilitated by NVivo 12.ResultsMajority of PCPs reported currently limited roles in managing acute and non-cancer issues, optimizing comorbidities and preventive care. PCPs aspired to expand their role to include cancer surveillance, risk assessment and addressing unmet psychosocial needs. PCPs preferred to harmonize cancer survivorship management of their primary care patients who are also BCS, with defined role distinct from oncologists. Training to understand the care protocol, enhancement of communication skills, confidence and trust were deemed necessary. PCPs proposed selection criteria of BCS and adequacy of their medical information; increased consultation time; contact details and timely access to oncologists (if needed) in the shared-care programme.ConclusionsPCPs were willing to share the care of BCS with oncologists but recommended role definition, training, clinical protocol, resources and access to oncologist's consultation to optimize the programme implementation