Comparison of SIVmac239(352–382) and SIVsmmPBj41(360–390) enterotoxic synthetic peptides

AbstractTo characterize the active domain of the simian immunodeficiency virus (SIV) surface unit (SU) enterotoxin, peptides corresponding to the V3 loop of SIVmac239 (SIVmac) and SIVsmmPBj41 (SIVpbj) were synthesized and examined for enterotoxic activity, α-helical structure, and interaction(s) with model membranes. SIVmac and SIVpbj induced a dose-dependent diarrhea in 6–8-day-old mouse pups similar to full-length SU. The peptides mobilized [Ca2+]i in HT-29 cells with distinct oscillations and elevated inositol triphosphate levels. Circular dichroism analyses showed the peptides were predominantly random coil in buffer, but increased in α-helical content when placed in a hydrophobic environment or with cholesterol-containing membrane vesicles that are rich in anionic phospholipids. None of the peptides underwent significant secondary structural changes in the presence of neutral vesicles indicating ionic interactions were important. These data show that the SIV SU enterotoxic domain localizes in part to the V3 loop region and interacts with anionic membrane domains on the host cell surface

Systematic review of respiratory viral pathogens identified in adults with community-acquired pneumonia in Europe

Community-acquired pneumonia (CAP) is an important respiratory disease and the fifth leading cause of mortality in Europe. The development of molecular diagnostic tests has highlighted the contributions of respiratory viruses to the aetiology of CAP, suggesting the incidence of viral pneumonia may have been previously underestimated. We performed a systematic review and meta analysis to describe the overall identification of respiratory viruses in adult patients with CAP in Europe, following PRISMA guidelines (PROSPERO; CRD42016037233). We searched EMBASE, MEDLINE, CINAHL, WHOLIS, COCHRANE library and grey literature sources for relevant studies, and screened these against protocol eligibility criteria. Two researchers performed data extraction and risk of bias assessments, independently, using a piloted form. Results were synthesised narratively, and random effects meta-analyses performed to calculate pooled estimates of effect; heterogeneity was quantified using I2.Twenty-eight studies met inclusion criteria of which 21 were included in the primary meta-analysis. The pooled proportion of patients with identified respiratory viruses was 22.0% (95% CI: 18.0%-27.0%), rising to 29.0% (25.0%–34.0%) in studies where polymerase chain reaction (PCR) diagnostics were performed. Influenza virus was the most frequently detected virus in 9% (7%-12%) of adults with CAP. Respiratory viruses make a substantial contribution to the aetiology of CAP in adult patients in Europe; one or more respiratory viruses are detected in about one quarter of all cases

Complex Systems Science and Community-Based Research

There is an abundance of community-based research literature that incorporates complex system science concepts and techniques. However, currently there is a gap in how these concepts and techniques are being used, and, more broadly, how these two fields complement one another. The debate on how complex systems science meaningfully bolsters the deployment of community-based research has not yet reached consensus, therefore, we present a protocol for a new scoping review that will identify characteristics at the intersection of community-based research and complex systems science. This knowledge will enhance the understanding of how complex systems science, a quickly evolving field, is being utilized in community-based research and practice

TRANSLATION AND VALIDATION OF THE HEALTHCARE TEAMWORK SURVEY QUESTIONNAIRE:STUDY DESIGN FOR THE MALAYSIAN CONTEXT

Cross-professional teamwork in healthcare is necessary to support integrated service delivery. However, cross-professional teamwork has not been given sufficient attention in developing country contexts. This paper presents a study design for translating and validating a teamwork survey questionnaire (TSQ) for the Malaysian context. The original TSQ was previously used in a developed country context. The tool will be translated into Malay which is the national language of Malaysia. Survey data will be subject to exploratory factor analysis to assess construct validity and Cronbach’s alpha test for reliability. The forward-backward translation approach of cross-cultural adaptation will be utilized. Two independent translators shall initially translate the questionnaire before reconciliation by the research team. The reconciled Malay version will then be back-translated into English. English translation will be contrasted against the original TSQ for further accuracy improvements in the Malay version. Face validation will be conducted with five academicians and five healthcare professionals to obtain feedback on necessary further adjustments. Upon finalizing the Malay version TSQ, interviews with service managers and senior healthcare professionals will be conducted to identify services with cross-professional teamwork at a designated hospital. A total of 150 respondents for survey validation will be recruited from identified services within the hospital. Different healthcare professionals having cross-communication and sharing patient care objectives will meet the criteria for a cross-professional team service. The validated Malay version TSQ could provide an invaluable tool for the assessment and improvement of cross-professional teamwork in the Malaysian healthcare context

An experience on natural sciences augmented reality contents for preschoolers

[EN] Early education is a key element for the future success of students in the education system. This work analyzes the feasibility of using augmented reality contents with preschool students (four and five years old) as a tool for improving their learning process. A quasi experimental design based on a nonequivalent groups posttest-only design was used. A didactic unit has been developed around the topic animals by the participant teachers. The control group followed all the didactic activities defined in the developed didactic materials, while the experimental group was provided in addition with some augmented reality contents. Results show improved learning outcomes in the experimental group with respect to the control group.The Spanish Ministry Economy and Competitiveness partially supported this work (Project ref. TIN2010-21296-C02-01).Cascales, A.; Laguna, I.; Pérez López, DC.; Perona Ruiz, PD.; Contero, M. (2013). An experience on natural sciences augmented reality contents for preschoolers. Lecture Notes in Computer Science. 8022:103-112. https://doi.org/10.1007/978-3-642-39420-1_12S1031128022Barnett, W.S.: Effectiveness of Early Educational Intervention. Science 333(6045), 975–978 (2011)OECD: Investing in high-quality early childhood education and care (ECEC). OECD Publishing, http://www.oecd.org/dataoecd/0/28/48980282.pdf (retrieved)Campos, P., Pessanha, S.: Designing Augmented Reality Tangible Interfaces for Kindergarten Children. In: Shumaker, R. (ed.) Virtual and Mixed Reality, HCII 2011, Part I. LNCS, vol. 6773, pp. 12–19. Springer, Heidelberg (2011)Lim, J., Kim, S.: A Study on Markerless AR-based Infant Education System using CBIR. Communications in Computer and Information Science 78, 52–58 (2010)Chen, C.H., Su, C.C., Lee, P.Y., Wu, F.G.: Augmented Interface for Children Chinese Learning Technologies. In: 7th IEEE International Conference on Advanced Learning Technologies, pp. 268–270. IEEE Press, New York (2007)Azuma, R.: A Survey of Augmented Reality. Presence: Teleoperators and Virtual Environments 6(1), 355–385 (1997)Winkler, T., Herczeg, M., Kritzenberger, H.: Mixed Reality Environments as Collaborative and Constructive Learning Spaces for Elementary School Children. In: Barker, P., Rebelsky, S. (eds.) Proceedings of World Conference on Educational Multimedia, Hypermedia and Telecommunications 2002, pp. 1034–1039. AACE, Chesapeake (2002)Hsieh, M.C., Lee, J.S.: AR Marker Capacity Increasing for Kindergarten English Learning. In: International Multi Conference of Engineers and Computer Scientists, vol. 1, pp. 663–666 (2008)Hsieh, M.C., Lin, H.C.K.: Interaction Design Based on Augmented Reality Technologies for English Vocabulary Learning. In: Wong, S.L., et al. (eds.) Proceedings of the 18th International Conference on Computers in Education, vol. 1, pp. 663–666. Asia-Pacific Society for Computers in Education (2010)Lee, H., Lee, J.: Mathematical Education Game Based on Augmented Reality. Technologies for E-Learning and Digital Entertainment, 442–450 (2008)Hyun, E., Choi, K., Kim, G.J., Han, J., Jo, M., Kim, N.: Delphi Survey on the Use of Robot Projector based Augmented Reality in Dramatic Activity for Young Children. International Journal of Digital Content Technology and its Applications 5(11), 272–282 (2011)Kim, H.M., Song, T.H., Jung, S.M., Kwon, K.H., Jeon, J.W.: Virtual Storyteller Using Marker Based AR and FPGA. In: IEEE 54th International Midwest Symposium on Circuits and Systems, pp. 1–4. IEEE Press, New York (2011)Dunleavy, M., Dede, C., Mitchell, R.: Affordances and Limitations of Immersive Participatory Augmented Reality Simulations for Teaching and Learning. Journal of Science Education and Technology 18, 7–22 (2009)Martín-Gutiérrez, J., Saorín, J.L., Contero, M., Alcañiz, M., Pérez-López, D., Ortega, M.: Design and validation of an augmented book for spatial abilities development in engineering students. Computers & Graphics 34(1), 77–91 (2010)Cook, T.D., Campbell, D.T., Day, A.: Quasi-experimentation: Design and Analysis Issues for Field Settings, pp. 19–21. Houghton Mifflin, Boston (1979)Buendía, L., Colás, P., Hernández-Pina, F.: Métodos de Investigación en Psicopedagogía. McGraw Hill, Madrid (1997

Potential site of Sungai Rawog conservation area, Sabah, as nature-based tourism destination in selected trails

Natural areas offer diverse resources, including those that support tourism in various regions. A comprehensive understanding of the characteristics and importance of these resources is imperative for decision-makers at different layers in order to formulate a suitable strategy for their implementation by management. Hence, the objective of this study was to conduct a survey and identify potential tourist attractions in selected trails within the Sungai Rawog Conservation Area. A total of 10 trails were chosen, including a main road in compartment 55, to assess the touristic potential product. Observations were made, and coordinates and pictures were taken and recorded. The findings revealed that noteworthy huge trees were found in several trails. It could be a pictorial spot with an extensive landscape forest view. Besides, the Kacip Fatimah plant was only available in Trail 2. The Nepenthes trail had an abundance of Nepenthes sp. within the area. The presence of a river nearby could also be a potential tourist attraction, such as river cruising and bird watching. Additionally, wildlife presence was recorded, including elephant excrement in the open area, sightings of eagles, and the nesting of orangutans on a tall tree branch. Therefore, the findings showed that it is crucial to identify the natural attributes as it shows the great potential as a nature-based tourism destination. Sustainable planning and collaborations between stakeholders are crucial for preserving the natural attributes