Computer simulation of web dynamics

Explicit finite difference schemes for modeling one-dimensional, transient web dynamics are designed, developed and tested. A sequence of numerical experiments are performed to ascertain the effects of various parameters on the stability and accuracy of the numerical results. Refinement of the numerical grid led to results which converged to analytical results for stable computations. For a particular web running speed and computational duration, there exists a time-step limit for stability. Longer computational time durations as well as higher web running speeds require reduced time-step size for stable numerical results. Upstream differencing of the Coriolis term to procure stability leads to numerical damping in the computations. A combination of upstream and central differencing yields stable results at larger time-steps than that required using full central differencing as well as less damping than that produced using full upstream differencing.Mechanical and Aerospace Engineerin

Inflammatory mediators act at renal pericytes to elicit contraction of vasa recta and reduce pericyte density along the kidney medullary vascular network

Introduction: Regardless of initiating cause, renal injury promotes a potent pro-inflammatory environment in the outer medulla and a concomitant sustained decrease in medullary blood flow (MBF). This decline in MBF is believed to be one of the critical events in the pathogenesis of acute kidney injury (AKI), yet the precise cellular mechanism underlying this are still to be fully elucidated. MBF is regulated by contractile pericyte cells that reside on the descending vasa recta (DVR) capillaries, which are the primary source of blood flow to the medulla. Methods: Using the rat and murine live kidney slice models, we investigated the acute effects of key medullary inflammatory mediators TNF-α, IL-1β, IL-33, IL-18, C3a and C5a on vasa recta pericytes, the effect of AT1-R blocker Losartan on pro-inflammatory mediator activity at vasa recta pericytes, and the effect of 4-hour sustained exposure on immunolabelled NG2+ pericytes. Results and discussion: Exposure of rat and mouse kidney slices to TNF-α, IL-18, IL-33, and C5a demonstrated a real-time pericyte-mediated constriction of DVR. When pro-inflammatory mediators were applied in the presence of Losartan the inflammatory mediator-mediated constriction that had previously been observed was significantly attenuated. When live kidney slices were exposed to inflammatory mediators for 4-h, we noted a significant reduction in the number of NG2+ positive pericytes along vasa recta capillaries in both rat and murine kidney slices. Data collected in this study demonstrate that inflammatory mediators can dysregulate pericytes to constrict DVR diameter and reduce the density of pericytes along vasa recta vessels, further diminishing the regulatory capacity of the capillary network. We postulate that preliminary findings here suggest pericytes play a role in AKI

WISE J072003.20-084651.2B Is A Massive T Dwarf

We present individual dynamical masses for the nearby M9.5+T5.5 binary WISE J072003.20$-$084651.2AB, a.k.a. Scholz's star. Combining high-precision CFHT/WIRCam photocenter astrometry and Keck adaptive optics resolved imaging, we measure the first high-quality parallactic distance ($6.80_{-0.06}^{+0.05}$ pc) and orbit ($8.06_{-0.25}^{+0.24}$ yr period) for this system composed of a low-mass star and brown dwarf. We find a moderately eccentric orbit ($e = 0.240_{-0.010}^{+0.009}$), incompatible with previous work based on less data, and dynamical masses of $99\pm6$ $M_{\rm Jup}$ and $66\pm4$ $M_{\rm Jup}$ for the two components. The primary mass is marginally inconsistent (2.1$\sigma$) with the empirical mass$-$magnitude$-$metallicity relation and models of main-sequence stars. The relatively high mass of the cold ($T_{\rm eff} = 1250\pm40$ K) brown dwarf companion indicates an age older than a few Gyr, in accord with age estimates for the primary star, and is consistent with our recent estimate of $\approx$70 $M_{\rm Jup}$ for the stellar/substellar boundary among the field population. Our improved parallax and proper motion, as well as an orbit-corrected system velocity, improve the accuracy of the system's close encounter with the solar system by an order of magnitude. WISE J0720$-$0846AB passed within $68.7\pm2.0$ kAU of the Sun $80.5\pm0.7$ kyr ago, passing through the outer Oort cloud where comets can have stable orbits.Comment: accepted to A

CMB-S4 Science Book, First Edition

This book lays out the scientific goals to be addressed by the next-generation ground-based cosmic microwave background experiment, CMB-S4, envisioned to consist of dedicated telescopes at the South Pole, the high Chilean Atacama plateau and possibly a northern hemisphere site, all equipped with new superconducting cameras. CMB-S4 will dramatically advance cosmological studies by crossing critical thresholds in the search for the B-mode polarization signature of primordial gravitational waves, in the determination of the number and masses of the neutrinos, in the search for evidence of new light relics, in constraining the nature of dark energy, and in testing general relativity on large scales

Near-infrared pyramid wavefront sensor for Keck adaptive optics: opto-mechanical design

A near-infrared, high order pyramid wavefront sensor will be implemented on the Keck telescope, with the aim of providing high resolution adaptive optics correction for the study of exoplanets around M-type stars and planet formation in obscured star forming regions. The pyramid wavefront sensor is designed to support adaptive optics correction of the light to an imaging vortex coronagraph and to a fiber injection unit that will feed a spectrograph. We present the opto-mechanical design of the near-infrared pyramid wavefront sensor, the optical performance, and the alignment strategy. The challenges of designing the assembly, as well as a fiber injection unit, to fit into the limited available space on the Keck adaptive optics bench, will also be discussed

Ubiquitylation in ERAD: Reversing to Go Forward?

Proteins are co-translationally inserted into the endoplasmic reticulum (ER) where they undergo maturation. Homeostasis in the ER requires a highly sensitive and selective means of quality control. This occurs through ER-associated degradation (ERAD).This complex ubiquitin-proteasome–mediated process involves ubiquitin conjugating enzymes (E2) and ubiquitin ligases (E3),lumenal and cytosolic chaperones, and other proteins, including the AAA ATPase p97 (VCP; Cdc48 in yeast). Probing of processes involving proteasomal degradation has generally depended on proteasome inhibitors or knockdown of specific E2s or E3s. In this issue of PLoS Biology, Ernst et al. demonstrate the utility of expressing the catalytic domain of a viral deubiquitylating enzyme to probe the ubiquitin system. Convincing evidence is provided that deubiquitylation is integral to dislocation of ERAD substrates from the ER membrane. The implications of this work for understanding ERAD and the potential of expressing deubiquitylating enzyme domains for studying ubiquitin-mediated processes are discussed

Innovations and advances in instrumentation at the W. M. Keck Observatory

Since the start of operations in 1993, the twin 10 meter W. M. Keck Observatory telescopes have continued to maximize their scientific impact and to produce transformative discoveries that keep the observing community on the frontiers of astronomical research. Upgraded capabilities and new instrumentation are provided though collaborative partnerships with Caltech and UC instrument development teams. The observatory adapts and responds to the observers’ evolving needs as defined in the observatory’s strategic plan, periodically refreshed in collaboration with the science community. This paper summarizes the performance of recently commissioned infrastructure projects, technology upgrades, and new additions to the suite of instrumentation at the observatory. We will also provide a status of projects currently in the design or development phase, and since we need to keep our eye on the future, we mention projects in exploratory phases that originate from our strategic plan. Recently commissioned projects include telescope control system upgrades, OSIRIS spectrometer and imager upgrades, and deployments of the Keck Cosmic Web Imager (KCWI), the Near-Infrared Echellette Spectrometer (NIRES), and the Keck I Deployable Tertiary Mirror (KIDM3). Under development are upgrades to the NIRSPEC instrument and adaptive optics (AO) system. Major instrumentation in design phases include the Keck Cosmic Reionization Mapper and the Keck Planet Finder. Future instrumentation studies and proposals underway include a Ground Layer Adaptive Optics system, NIRC2 upgrades, the energy sensitive instrument KRAKENS, an integral field spectrograph LIGER, and a laser tomography AO upgrade. Last, we briefly discuss recovering MOSFIRE and its return to science operations