593 research outputs found

    Oscillatory electrostatic potential on graphene induced by group IV element decoration

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    The structures and electronic properties of partial C, Si and Ge decorated graphene were investigated by first-principles calculations. The calculations show that the interaction between graphene and the decoration patches is weak and the semiconductor patches act as agents for weak electron doping without much disturbing graphene electronic π-bands. Redistribution of electrons due to the partial decoration causes the electrostatic potential lower in the decorated graphene areas, thus induced an electric field across the boundary between the decorated and non-decorated domains. Such an alternating electric field can change normal stochastic adatom diffusion to biased diffusion, leading to selective mass transport

    Search for the Lepton Flavor Violation Process J/ψeμJ/\psi \to e\mu at BESIII

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    We search for the lepton-flavor-violating decay of the J/ψJ/\psi into an electron and a muon using (225.3±2.8)×106(225.3\pm2.8)\times 10^{6} J/ψJ/\psi events collected with the BESIII detector at the BEPCII collider. Four candidate events are found in the signal region, consistent with background expectations. An upper limit on the branching fraction of B(J/ψeμ)<1.5×107\mathcal{B}(J/\psi \to e\mu)< 1.5 \times 10^{-7} (90% C.L.) is obtained

    Visualization and real-time fluorescence RPA detection methods for enterohemorrhagic Escherichia coli O157:H7

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    ObjectiveTo detect enterohemorrhagic Escherichia coli (EHEC) O157:H7, visualization recombinant enzyme polymerase amplification (RPA) detection and real-time fluorescence RPA detection methods were established based on RPA technology.MethodsPrimers and probes were designed based on the rfbE gene of EHEC O157:H7, and specificity and sensitivity were determined. A real-time fluorescent RPA method was established. Combined with the color change characteristics of SYBR Green I in nucleic acid reactions, a visualization RPA method for the detection of EHEC O157:H7 was established.ResultsThe established real-time fluorescence RPA detection and visual RPA detection methods both detected EHEC O157:H7 in 25 min at a reaction temperature of 35 ℃. The designed primers and probes showed specific results only for EHEC O157:H7. The detection limits of the two methods were lower than 10-5 ng/μL.ConclusionThe visual RPA detection and real-time fluorescence RPA detection methods of EHEC O157:H7 were easy to operate with strong specificity and high sensitivity. This study provides novel methods for the rapid detection of EHEC O157:H7 in the field and laboratory

    Search for Baryonic Decays of \psi(3770) and \psi(4040)

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    By analyzing data samples of 2.9 fb^{-1} collected at \sqrt s=3.773 GeV, 482 pb^{-1} collected at \sqrt s=4.009 GeV and 67 pb^{-1} collected at \sqrt s=3.542, 3.554, 3.561, 3.600 and 3.650 GeV with the BESIII detector at the BEPCII storage ring, we search for \psi(3770) and \psi(4040) decay to baryonic final states, including \Lambda\bar\Lambda\pi^+\pi^-, \Lambda \bar\Lambda\pi^0, \Lambda\bar\Lambda\eta, \Sigma^+ \bar\Sigma^-, \Sigma^0 \bar\Sigma^0, \Xi^-\bar\Xi^+ and \Xi^0\bar\Xi^0 decays. None are observed, and upper limits are set at the 90% confidence level.Comment: 9 pages, 3 figure

    A genome-wide association study of early menopause and the combined impact of identified variants

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    Early menopause (EM) affects up to 10% of the female population, reducing reproductive lifespan considerably. Currently, it constitutes the leading cause of infertility in the western world, affecting mainly those women who postpone their first pregnancy beyond the age of 30 years. The genetic aetiology of EM is largely unknown in the majority of cases. We have undertaken a meta-analysis of genome-wide association studies (GWASs) in 3493 EM cases and 13 598 controls from 10 independent studies. No novel genetic variants were discovered, but the 17 variants previously associated with normal age at natural menopause as a quantitative trait (QT) were also associated with EM and primary ovarian insufficiency (POI). Thus, EM has a genetic aetiology which overlaps variation in normal age at menopause and is at least partly explained by the additive effects of the same polygenic variants. The combined effect of the common variants captured by the single nucleotide polymorphism arrays was estimated to account for ∼30% of the variance in EM. The association between the combined 17 variants and the risk of EM was greater than the best validated non-genetic risk factor, smokin

    The 5th International Conference on Biomedical Engineering and Biotechnology (ICBEB 2016)

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    Construction and Characterization of a Bacterial Artificial Chromosome Library for the Hexaploid Wheat Line 92R137

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    For map-based cloning of genes conferring important traits in the hexaploid wheat line 92R137, a bacterial artificial chromosome (BAC) library, including two sublibraries, was constructed using the genomic DNA of 92R137 digested with restriction enzymes HindIII and BamHI. The BAC library was composed of total 765,696 clones, of which 390,144 were from the HindIII digestion and 375,552 from the BamHI digestion. Through pulsed-field gel electrophoresis (PFGE) analysis of 453 clones randomly selected from the HindIII sublibrary and 573 clones from the BamHI sublibrary, the average insert sizes were estimated as 129 and 113 kb, respectively. Thus, the HindIII sublibrary was estimated to have a 3.01-fold coverage and the BamHI sublibrary a 2.53-fold coverage based on the estimated hexaploid wheat genome size of 16,700 Mb. The 765,696 clones were arrayed in 1,994 384-well plates. All clones were also arranged into plate pools and further arranged into 5-dimensional (5D) pools. The probability of identifying a clone corresponding to any wheat DNA sequence (such as gene Yr26 for stripe rust resistance) from the library was estimated to be more than 99.6%. Through polymerase chain reaction screening the 5D pools with Xwe173, a marker tightly linked to Yr26, six BAC clones were successfully obtained. These results demonstrate that the BAC library is a valuable genomic resource for positional cloning of Yr26 and other genes of interest
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