Gastric pseudoaneurysm in the setting of Loey’s Dietz Syndrome

Loey’s Dietz syndrome is a disorder of connective tissue caused by a mutation in the genes that encode transforming growth factor (TGF) beta receptor 1 and 2. It is an autosomal dominant disorder similar to Marfan’s syndrome but with a more aggressive clinical course. Patients with Loey’s-Dietz syndrome have progressive dilatation of the aortic root that can lead to aortic dissection and rupture. The location of non-aortic arterial aneurysms may be wide spread but often occur in the head and neck vessels.peer-reviewe

Psychosexual aspects of vulvovaginal pain

Vulvovaginal pain problems are major health concerns in women of childbearing age. Controlled studies have shown that vulvovaginal pain can adversely affect women and their partners’ general psychological well-being, relationship adjustment and overall quality of life. These women have significantly lower levels of sexual desire, arousal, and satisfaction, as well as a lower intercourse frequency than normal controls. They also report more anxiety and depression, in addition to more distress about their body image and genital self-image. Empirical studies indicate that specific psychological and relationship factors may increase vulvovaginal pain intensity and its psychosexual sequelae. Randomized clinical trials have shown that psychosexual interventions, namely cognitive-behavioral therapy (CBT), are efficacious in reducing vulvovaginal pain and improving associated psychosexual outcomes. Women reporting significant psychological, sexual and/or relationship distress should be referred for psychosexual treatment. A multimodal approach to care integrating psychosexual and medical management is thought to be optimal

Feasibility Study of Liquid-Based Cytology for Post-Treatment Surveillance of Patients with Vulvar Intraepithelial Neoplasia

Introduction: Colposcopy and tissue biopsy remain the gold standard for diagnosing vulvar intraepithelial neoplasia (VIN). Vulvar colposcopy is fairly nonspecific. As a result, many women undergo unwarranted painful biopsies. Vulvar cytology, which is relatively painless, inexpensive and allows a larger area to be sampled, may likely reduce false negative diagnoses. However, previous cytological studies that used conventional methodologies were largely unsuccessful in diagnosing VIN. In this study, liquid based cytology and HPV typing by PCR have been assessed as possible alternatives to biopsy for follow-up surveillance of women treated for VIN. Methods: Women with a history of VIN and a control group were recruited from a colposcopy clinic. Clinically suspicious lesions and normal vulvar tissue were vigorously brushed and the sample collected in PreservCyt® fluid for cytologic examination and for HPV-typing with a multiplex PCR assay, using primers designated PGMY09/11. Samples (N=82) were obtained from 52 lesions clinically suspicious for VIN, 15 controls from the same women in areas of the vulva with no clinical abnormality, and 15 controls from women with no current clinical evidence or past history of VIN. Concurrent tissue biopsies were obtained, immediately after brushing, from the 52 clinically suspicious VIN lesions. A single pathologist read and interpreted the biopsy samples. To ensure unbiased testing, cytology and HPV analyses were performed at separate independent laboratories by professionals blinded to clinical findings and biopsy results. Cytology was coded as negative, ASCUS, VIN I, VIN 2, or VIN 3. Specificity, sensitivity, and predictive values of cytology and HPV for VIN were calculated. Fisher’s exact was used to determine associations between HPV and cytology. Logistic regression was done to determine if cytology or HPV predicted tissue biopsy results. Results: Vulvar samples (N=82) were collected from 48 women aged 19-65 who participated in this study. Histology results of the 52 lesions clinically suspected as VIN were reported as follows: VIN I (n=33), VIN 2/3 (n=13), benign (n=4), contact dermatitis and condyloma (n=1 each). Ninety percent of the vulvar samples were adequate for cytologic evaluation, but only 72% of samples had adequate cellularity for HPV testing. Sensitivity, specificity and positive predictive value (PPV) of vulvar cytology for recurrent VIN were 95%, 15% and 65% respectively. PCR for HPV, when independently correlated with histology, had, 62% sensitivity, 85% specificity and 89% PPV for VIN. No significant associations were found between cytology and HPV (p=0.3559). Neither cytology nor HPV predicted pathological diagnosis of VIN. Conclusion: By vigorous brushing, it is possible to obtain an adequate cellular sample from the vulva for cytologic and/or molecular evaluation for HPV.The specificity for VIN at cytology was not satisfactory for use as a clinical alternative to biopsy to detect recurrent VIN. This may be due to the small sample size, difficulty in accurately grading vulvar dysplasia at cytology, and possible differences in cytomorphologic criteria for diagnosing dysplasia in the vulva as compared to the cervix. This study will be further refined with the development of more reproducible consensus criteria for cytologic evaluation of VIN. A larger number of participants could shed more light on the significance of cytology with or without HPV testing as an alternative to tissue biopsy for follow up of patients with VIN

The relationship between pelvic organ prolapse, genital body image, and sexual health

Aims Pelvic organ prolapse involves physical changes to the genitals, potentially distressing to women. We hypothesized poorer genital body image in prolapsed women versus controls and that genital body image would correlate with sexual health. Methods Seventy‐four sexually active women, 13 with prolapse, 24 with surgically corrected prolapse, 37 without prolapse, completed the Genital Self Image Scale (GSIS‐20), Body Esteem Scale (BES), and Female Sexual Function Index (FSFI). Results In prolapsed women median GSIS‐20 scores were 28/40, women with surgically corrected prolapse 32/40 and never prolapsed 34/40 (χ 2  = 9.6, P  < 0.01). Post hoc analysis showed significant differences between prolapsed and never prolapsed groups ( P  < 0.05). After adjusting for BES, GSIS‐20 correlated with overall FSFI (r = 0.384, P  < 0.01), and its subscales of desire (r =0.34, P  < 0.05) and satisfaction (r = 0.41, P  < 0.01). Conclusions Women with prolapse are at risk for poorer genital body image and reduced sexual health. Neurourol. Urodynam. 31:1145–1148, 2012. © 2012 Wiley Periodicals, Inc.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/93520/1/22205_ftp.pd

CTS e transgênicos : uma proposta de sequência didática para o ensino de biotecnologia no ensino médio

Orientador: Prof.ª. Drª. Luciane Viater TureckMonografia (Licenciatura) - Universidade Federal do Paraná, Setor de Ciências Biológicas, Curso de Graduação em Ciências BiológicasInclui referênciasResumo : Este trabalho apresenta uma proposta pedagógica para o ensino de biotecnologia no ensino médio, fundamentada nos princípios da abordagem Ciência, Tecnologia e Sociedade (CTS) e alinhada à Base Nacional Comum Curricular (BNCC), que incentiva práticas educativas críticas e contextualizadas. A literatura da área identifica a importância de desenvolver nos estudantes competências e habilidades como o pensamento crítico, letramento científico e protagonismo, tendo a área de ciências da natureza o potencial para conectar os conteúdos técnicos a contextos sociais e éticos para alcançar estes objetivos. Nesse contexto, a proposta elaborada busca promover uma compreensão integrada da biotecnologia. A proposta está organizada em cinco etapas, tendo por base metodológica e teórica a abordagem baseada nas relações entre CTS, partindo portando da problematização inicial até a síntese e avaliação como etapa final da proposta. As práticas propostas em cada etapa buscam, a partir de atividades integradoras e reflexivas, estimular a participação ativa e a análise crítica dos e das estudantes. A opção por adotar a abordagem CTS surge do potencial da mesma em integrar conceitos científicos e tecnológicos a realidade social, trazendo o contexto e o alcance destes conceitos em seus aspectos sociais para a sala de aula. Tais aspectos são essenciais ao se tratar do tema Organismos Geneticamente Modificados (OGM), que se insere no tópico Biotecnologia, já que o mesmo possui implicações complexas em nível de ambiente, saúde e social. Além de atender às diretrizes da BNCC, espera-se que a proposta se mostre flexível e adaptável a diferentes contextos escolares, oferecendo um modelo que pode ser ampliado para outros conteúdos científicos. O trabalho também sugere caminhos para a ampliação da abordagem CTS no ensino de biotecnologia, incluindo a necessidade de capacitação docente e a integração interdisciplinar, ao mesmo tempo em que propõe reflexões para futuras pesquisas sobre a eficácia dessas metodologias em outras áreas do currículo escola

A dança vogue femme : análise cinesiológica do elemento dip na articulação do joelho

O Vogue Femme é uma dança que surgiu sob a forma de batalhas performativas, por volta da década de 60, em bailes da comunidade afro-americana e latina LGBTQ+. O Dip, é o elemento mais icônico do Vogue Femme e, também, um dos mais complicados de ser executado, podendo ocasionar lesões na articulação do joelho devido à posição dessa articulação na finalização do movimento. Apesar do aumento da popularidade da cultura Ballroom, nos últimos 20 anos, ainda há pouco conhecimento no mundo acadêmico sobre a saúde de um Vogue performer. Assim, com esse estudo pretende abrir portas para uma discussão sobre a saúde da comunidade, visto que muitas lesões ocorrem pela falta de informação e pela falta de estudos e publicações nessa área. Esse estudo tem como objetivo geral realizar uma análise cinesiológica qualitativa da articulação do joelho no Dip; e, como objetivos específicos: verificar a musculatura da articulação do joelho envolvida durante a execução do elemento Dip; e, descrever os ângulos da articulação do joelho ao longo da execução do Dip. Essa pesquisa é um estudo de caso do tipo descritivo. O participante estudado é um Vogue Performer selecionado de forma não-probabilística intencional. Para a captura do elemento Dip foi utilizada uma câmera de vídeo GoPro HERO 7 Black, resolução de 1920x1080p, 60 frames por segundo, posicionada a dois metros de distância do bailarino, possibilitando uma visão do elemento em 2D no plano coronal e no sagital. Para a análise do vídeo de captura do elemento Dip foi utilizado o software Kinovea, versão 0.9.4, no qual puderam ser observados os movimentos articulares do joelho e calculados os ângulos articulares em ambos os lados do corpo nas diferentes etapas de execução do elemento. Após a coleta de dados, a análise cinesiológica foi realizada através da observação das imagens capturadas. Para essa análise, o elemento Dip foi dividido em quatro fases, identificando os grupos musculares primários, nas fases excêntrica e concêntrica, atuantes na articulação do joelho e os ângulos articulares do joelho durante a sua execução. Em relação aos músculos primários envolvidos ao longo da execução do Dip, na fase 2 e 3 o grupo muscular quadríceps (músculos reto femoral, vasto lateral, vasto intermédio e vasto medial) realiza uma contração excêntrica; e, na fase 4 os mesmos músculos seguem agindo de forma excêntrica no joelho direito e concêntrica no joelho esquerdo. Em relação aos ângulos atingidos ao longo da execução do elemento Dip, observou-seVogue Femme is a dance that arises from performatic battles that took place in afroamerican community balls around the 1960s. The Dip is the most iconic Vogue Femme element and is also one of the most complicated to execute, with the potential to cause injury to the knee joint due to the position of the knee in the ending pose of the Dip. Although the popularity of Ballroom culture has increased in the last 20 years, there is still little works about it published in the academic field regarding the health of a Vogue Performer. Therefore, this study inteds to create discussions about the Ballroom community health, since many injuries occur due to the lack of information and studies in the Vogue Femme field. The main object of this work is to perform a qualitative kinesiological analysis of the knee joint in the Dip. The specific objectives are to verify the musculature of the knee joint, describing and documentating its angles, during the execution of the Dip element. This paper is a descriptive case study. The studied subject is a Vogue Performer selected in an intentional non-probabilistic manner. To capture the Dip element, a GoPro HERO 7 Black video camera was used (1920x1080p resolution, 60 frames per second), being positioned two meters far from the dancer, allowing a 2D view of the element in the coronal and sagittal planes. Kinovea software (version 0.9.4) was used to analyze the video of the Dip element, in which the knee joint movements could be observed and the joint angles on both sides of the body were calculated in the different stages of the execution. After data collection, kinesiological analysis was performed by observing the captured images. For the analysis, the Dip element was divided into four phases, identifying the primary muscle groups in both eccentric and concentric phases, that act on the knee joint and the knee joint angles during its execution. Regarding the primary muscles involved throughout the execution of the Dip in phase 2 and 3, the quadriceps muscles (rectus femoris, vastus lateralis, vastus intermedius and vastus medialis) perform an eccentric contraction; and, during phase 4, the same muscles continue to act eccentrically in the right knee and concentric in the left knee. Regarding the angles reached during the execution of the Dip element, an average angulation of 45º in the right knee and 97º in the left knee was detected in phase 2; 20º on the right knee and 94º on the left knee in phase 3; and 18º in the right knee and 0º in the left knee in phase 4. After carrying out this research, it is possible to better understand the kinesiological characteristics of the Dip element, and to find a means of preventing injuries, therefore assisting in the teaching of the Vogue Femme dance and contribuiting as reference for future studies

A comparison of two methods to determine percent body fat

The purpose of this investigation was to compare percent body fat determined by a two-component (2C) model using densitometry alone (PFD) to percent body fat determined by a three-component (3C) model using body density plus total body water (PFDW). In addition, the water content of the fat-free mass (TBW/FFM) using FFM values from both methods (2C, 3C) was compared among three ethnicities, African American (males n=11, females n=l0), Caucasian (males n=12, females n=22) and Hispanic (males n=l0, females n=8), and among three age groups, 18 - 35 (males n=33, females n=40), 36-59 (males n=23, females n=24), and 61-80 (males n=13, females n=l0). Also compared were the TBW/FFM (2C, 3C) of higher vs lower percent fat males and females. Body density (Db) was determined by hydrostatic weighing at residual lung volume, and total body water (TBW) was measured using deuterium oxide dilution techniques. Intraclass correlations and analysis of variance were used to compare PFD to PFDW for both females and males. For females, PFD was 27.4 � 9.7, and PFDW was 28.7 � 9.0. For males, PFD was 18.1 � 8.7, and PFDW was 20.6 � 8.1. The two methods correlated highly for both males (R1 = .958) and females (R1 = .972). Despite the high R1 values, PFDW was significantly greater than PFD (p.01) for both males and females. No significant differences were found for TBW/FFM for either method among the three ethnic groups, among young, middle and older age groups, or between the low percent fat and high percent fat groups of males and females. It is concluded from this study that percent body fat from hydrodensitometry produces a value that correlates highly to percent body fat determined from an established multicomponent method using body density and total body water. Even though multicomponent models have been recommended (6), hydrodensitometry can still be considered an acceptable method for determining percent body fat in healthy individuals because FFM hydration appears to remain stable across age and ethnic groups, and in subjects with varying degrees of body fatness.California State University, Northridge. Department of Kinesiology.Includes bibliographical references (leaves 15-16, 30-32

The betaine and choline content of a whole wheat flour compared to other mill streams

Diets that contain high levels of whole grains have been associated with a reduced risk of several chronic diseases (Marquart et al., 2002). A whole grain, as defined by AACC International, should consist of the bran, germ and endosperm in the same proportions as found in nature (AACC, 1999). Whole grains are an important source of dietary fiber, resistant starch, vitamins, minerals and other bioactive nutrients that can play a role in protecting against chronic disease (Slavin, 2003). One of these nutrients is betaine (also known as trimethyl glycine or glycine betaine), an osmolyte and methyl donor shown to protect internal organs and improve vascular risk factors (Craig, 2004). As an osmolyte, it helps protect a variety of cells from osmotic stress. Betaine also plays an important role in cell biochemistry, donating a methyl group to homocysteine to produce methionine and ultimately S-adenosyl-methionine. Betaine accumulates in many plants under stress from drought, high salinity or low temperature. Cereal plants with betaine accumulation include wheat, barley and rye; those with little or no accumulation include rice, millet and sorghum (Kishitani et al., 1994)