Study of individual erythrocyte deformability susceptibility to INFeD and ethanol using a microfluidic chip

Human red blood cells (RBCs) deformability in vitro was assessed during iron dextran (INFeD) loading and/or ethanol co-administration using microfluidic deformability screening. The results showed donor-specific variations in dose dependent deformability shift were revealed below 500 μg/mL iron dextran. Two out of nine blood samples exhibited significant cell stiffening at 500 μg/mL iron dextran loading concentration (p < 0.05, Tukey test). More interestingly, co-administration of moderate amount of ethanol was identified to have significant protective effects on RBC deformability. We also noted that ethanol can reverse the deformability of impaired RBCs. Meanwhile obvious donor dependent response to ethanol administration on RBC deformability was noted using our biomimetic microfluidic chip.Singapore-MIT Alliance in Research and Technology (SMART

Real-time observation and control of optical chaos

Optical chaotic system is a central research topic due to its scientific importance and practical relevance in key photonic applications such as laser optics and optical communication. Because of the ultrafast propagation of light, all previous studies on optical chaos are based on either static imaging or spectral measurement, which shows only time-averaged phenomena. The ability to reveal real-time optical chaotic dynamics and, hence, control its behavior is critical to the further understanding and engineering of these systems. Here, we report a real-time spatial-temporal imaging of an optical chaotic system, using compressed ultrafast photography. The time evolution of the system’s phase map is imaged without repeating measurement. We also demonstrate the ability to simultaneously control and monitor optical chaotic systems in real time. Our work introduces a new angle to the study of nonrepeatable optical chaos, paving the way for fully understanding and using chaotic systems in various disciplines

Analysis of the Extracurricular Sports to Promote the Healthy Development of Students’ Physique

In recent years during the process of Students education, a healthy body is fundamental to learning, but it is also a problem that is often ignored. Especially for college students, they are the future and hope for the construction of the motherland and they bear an important responsibility of national construction in the future. (Bing Li, 2012) So it is necessary to strengthen and promote the physical health of college students. This paper uses the methods of literature, questionnaire surveys and other scientific research methods to study the health development of students’ physical health in Northeast Petroleum University, analyzes the status quo and influencing factors of students’ physical health in Northeast Petroleum University, and discusses the way of after class sports promoting healthy development of the students, and putting forward corresponding countermeasures to further improve the physical health level of the students of Northeast Petroleum University, and provide a theoretical reference for the cultivation of a good consciousness of lifelong physical exercise

A signal cascade originated from epidermis defines apical-basal patterning of Arabidopsis shoot apical meristems

In multicellular organisms, a long-standing question is how spatial patterns of distinct cell types are initiated and maintained during continuous cell division and proliferation. Along the vertical axis of plant shoot apical meristems (SAMs), stem cells are located at the top while cells specifying the stem cells are located more basally, forming a robust apical-basal pattern. We previously found that in Arabidopsis SAMs, the HAIRY MERISTEM (HAM) family transcription factors form a concentration gradient from the epidermis to the interior cell layers, and this gradient is essential for the stem cell specification and the apical-basal patterning of the SAMs. Here, we uncover that epidermis specific transcription factors, ARABIDOPSIS THALIANA MERISTEM LAYER 1 (ATML1) and its close homolog, define the concentration gradient of HAM in the SAM through activating a group of microRNAs. This study provides a molecular framework linking the epidermis-derived signal to the stem cell homeostasis in plants

Reduced cytotoxicity of silver ions to mammalian cells at high concentration due to the formation of silver chloride

Silver-containing antimicrobial agents are used in various medical products. However, their toxicity to mammalian cells has not been sufficiently evaluated. Numerous studies have unveiled evidence of significant antimicrobial properties associated with Ag ions. In cell culture media or human body fluids, the free Ag+ has rich opportunities to complex with Cl-. Surprisingly, studies on the toxicity of solid form AgCl(s) to mammalian cells are quite limited. In this study, we evaluated the cytotoxicity of Ag ions and silver chloride colloids on red blood cells and human mesenchymal stem cells (hMSCs). The adverse effects of silver chloride on red blood cells and hMSC were viewed by SEM and LIVE/DEAD viability staining, respectively. Among different tested chemical forms of silver, AgCl was identified to be the least cytotoxic. Moreover, a decline in the cytotoxicity of AgCl at significantly high concentrations was observed. We attributed the reduced cytotoxicity to aggregated AgCl which limited the bioavailability of free Ag+ ions

A stream processing framework based on linked data for information collaborating of regional energy networks

© 2005-2012 IEEE. Coordinating of energy networks to form a city-level multidimensional integrated energy system becomes a new trend in Energy Internet (EI). The collaborating in the information layer is a core issue to achieve smart integration. However, the heterogeneity of multiagent data, the volatility of components, and the real-time analysis requirement in EI bring significant challenges. To solve these problems, in this article we propose a stream processing framework based on linked data for information collaboration among multiple energy networks. The framework provides a universal data representation based on linked data and semantic relation discovery approach to model and semantically fuse heterogeneous data. Semantics-based information transmission contracts and channels are automatically generated to adapt to structural changes in EI. A multimodel-based dynamic adjusting stream processing is implemented using data semantics. A real-world case study is implemented to demonstrate the adaptability, feasibility, and flexibility of the proposed framework

Effects of Dietary Protein and Energy Levels on Growth Performance, Nutrient Digestibility, and Serum Biochemical Parameters of Growing Male Mink (Neovison vison)

The objective of this experiment was to determine the optimum dietary metabolic energy (ME) and crude protein (CP) levels of growing male mink. One hundred forty-four healthy male minks at 75 days were randomly allocated into the six groups with 24 replicates, which was one mink for each replicate. The mink were fed six experimental diets with two CP levels (31.59 and 35.63%) and three ME levels (14.17, 15.96, and 17.73 MJ/kg) for a 7-day preliminary period and then for an 88-day experimental period. The final body weight (BW), average daily gain (ADG), feed conversion ratio (FCR), fat digestibility, energy intake, the concentration of glucose (GLU), and low-density lipoprotein (LDL) of the mink were significantly increased by the CP or ME levels (P &lt; 0.05). In addition, CP levels significantly (P &lt; 0.01) increased the N intake and N retention. Dietary ME levels increased the utilization of gross energy. Obviously, there were significant CP × ME interactions for the final BW, ADG, fat digestibility, energy utilization, GLU, LDL (P &lt; 0.01), and triglyceride contents (P &lt; 0.05). Therefore, the optimum CP and ME levels were 35.97% and 18.18 MJ/kg, which can improve growth, enhance nutrient digestion, and promote blood lipid metabolism in growing mink