Genetic and environmental influences on in vitro digestibility of alfalfa

To study the relationships between in vitro NDF or true dry matter digestibility (NDFD and IVTDMD) and forage fibre content, 95 alfalfa samples from 5 cultivars grown in 3 different locations and from different cuts were analysed for fibre fractions and evaluated for NDFD and IVTDMD. NDFD was mainly influenced by order of cut and age of the fields. The multifoliate cultivar controlled did not appear to differ for fibre composition and NDFD from the other ones. Fibre fractions contents explained a large part of the difference in NDFD and IVTDMD, and no significant differences in this relationships appeared for first-cut vs. aftermath forage. The best predictive equation of NDFD from fibre factions was: NDFD = 73.61 -0.62 * NDF (% DM) – 56.33 * ADL/NDF (R2=0.39). Variations in fibre content and quality do not completely explain differences in NDFD suggesting the interference of other factors that are worth to be better studied

Beam test calibration of the balloon-borne imaging calorimeter for the CREAM experiment

CREAM (Cosmic Ray Energetics And Mass) is a multi-flight balloon mission designed to collect direct data on the elemental composition and individual energy spectra of cosmic rays. Two instrument suites have been built to be flown alternately on a yearly base. The tungsten/Sci-Fi imaging calorimeter for the second flight, scheduled for December 2005, was calibrated with electron and proton beams at CERN. A calibration procedure based on the study of the longitudinal shower profile is described and preliminary results of the beam test are presented.Comment: 4 pages, 4 figures. To be published in the Proceedings of 29th International Cosmic Ray Conference (ICRC 2005), Pune, India, August 3-10, 200

Type 3 hypersensitivity in COVID-19 vasculitis

Coronavirus Disease 2019 (COVID-19) is an ongoing public health emergency and new knowledge about its immunopathogenic mechanisms is deemed necessary in the attempt to reduce the death burden, globally. For the first time in worldwide literature, we provide scientific evidence that in COVID-19 vasculitis a life-threatening escalation from type 2 T-helper immune response (humoral immunity) to type 3 hypersensitivity (immune complex disease) takes place. The subsequent deposition of immune complexes inside the vascular walls is supposed to induce a severe inflammatory state and a cytokine release syndrome, whose interleukin-6 is the key myokine, from the smooth muscle cells of blood vessels

Design and Studies of μ-strip Stacked Module Prototypes for Tracking at Super-LHC

AbstractExperience at high luminosity hadrons collider experiments shows that tracking information enhances the trigger rejection capabilities while retaining high efficiency for interesting physics events. The design of a tracking based trigger for Super LHC (S-LHC), the already envisaged high luminosity upgrade of the LHC collider, is an extremely challenging task, and requires the identification of high-momentum particle tracks as a part of the Level 1 Trigger.Simulation studies show that this can be achieved by correlating hits on two closely spaced silicon strip sensors. The progresses on the design and development of this micro-strip stacked prototype modules and the performance of few prototype detectors will be presented. The prototypes have been built with the silicon sensors and electronics used to equip the present CMS[1] Tracker.Preliminary results of a simulated tracker layout equipped with stacked modules are discussed in terms of pT resolution and triggering capabilities.The study of real prototypes in terms of signal over noise and tracking performance with cosmic rays and a dedicated beam test experiment will also be shown

Clinical outcome of kidney transplantation in HIV-infected recipients: a retrospective study

Kidney transplantation is a safe and effective option for HIV-positive (HIV+) patients. We conducted a retrospective study on HIV+ kidney transplant recipients who underwent transplantation from March 2008 to September 2016.Inclusion criteria for transplantation were CD4þ T-cell count 200 per mm3 and undetectable HIV load. The current study reports the outcome of 19 HIV+ recipients, mostly of Caucasian origin (79%) with a median age of 50 years (interquartile range [IQR], 42–52), who were followed up for a median period of 2.4 years (IQR, 1.2–4.6) after transplantation. Compared with HIV-negative (HIV-) controls, HIV+ recipients had similar one- and three-year graft and patient survival, but significantly lower five-year patient survival (P¼0.03). The differences in graft outcome became less evident with the analysis of death-censored graft survival rates. Cumulative incidence of allograft rejection at one year was 32.9%. Rates of infections were not particularly elevated and HIV replication remained well controlled in all but one patient. A high prevalence of metabolic and endocrine complications (68%) was reported after transplantation. Further studies are needed to evaluate long-term outcomes of HIV+ recipients who underwent kidney transplantation

Coprophagous features in carnivorous Nepenthes plants: a task for ureases

Most terrestrial carnivorous plants are specialized on insect prey digestion to obtain additional nutrients. Few species of the genus Nepenthes developed mutualistic relationships with mammals for nitrogen supplementation. Whether dietary changes require certain enzymatic composition to utilize new sources of nutrients has rarely been tested. Here, we investigated the role of urease for Nepenthes hemsleyana that gains nitrogen from the bat Kerivoula hardwickii while it roosts inside the pitchers. We hypothesized that N. hemsleyana is able to use urea from the bats’ excrements. In fact, we demonstrate that 15N-enriched urea provided to Nepenthes pitchers is metabolized and its nitrogen is distributed within the plant. As ureases are necessary to degrade urea, these hydrolytic enzymes should be involved. We proved the presence and enzymatic activity of a urease for Nepenthes plant tissues. The corresponding urease cDNA from N. hemsleyana was isolated and functionally expressed. A comprehensive phylogenetic analysis for eukaryotic ureases, including Nepenthes and five other carnivorous plants’ taxa, identified them as canonical ureases and reflects the plant phylogeny. Hence, this study reveals ureases as an emblematic example for an efficient, low-cost but high adaptive plasticity in plants while developing a further specialized lifestyle from carnivory to coprophagy

Results on Multiple Coulomb Scattering from 12 and 20 GeV electrons on Carbon targets

Multiple scattering effects of 12 and 20 GeV electrons on 8 and 20 mm thickness carbon targets have been studied with high-resolution silicon microstrip detectors of the UA9 apparatus at the H8 line at CERN. Comparison of the scattering angle between data and GEANT4 simulation shows excellent agreement in the core of the distributions leaving some residual disagreement in the tails.Comment: 14 pages, 16 figures. Updated to match published versio

In vitro efficacy and safety of a system for sorbent-assisted peritoneal dialysis

In vitro efficacy and safety of a system for sorbent-assisted peritoneal dialysis. Am J Physiol Renal Physiol 319: F162-F170, 2020. First published June 1, 2020; doi:10.1152/ajprenal. 00079.2020.-A system for sorbent-assisted peritoneal dialysis (SAPD) was designed to continuously recirculate dialysate via a tidal mode using a single lumen peritoneal catheter with regeneration of spent dialysate by means of sorbent technology. We hypothesize that SAPD treatment will maintain a high plasma-to-dialysate concentration gradient and increase the mass transfer area coefficient of solutes. Thereby, the SAPD system may enhance clearance while reducing the number of exchanges. Application is envisaged at night as a bedside device (12 kg, nighttime system). A wearable system (2.0 kg, daytime system) may further enhance clearance during the day. Urea, creatinine, and phosphate removal were studied with the daytime and nighttime system (n = 3 per system) by recirculating 2 liters of spent peritoneal dialysate via a tidal mode (mean flow rate: 50 and 100 mL/min, respectively) for 8 h in vitro. Time-averaged plasma clearance over 24 h was modeled assuming one 2 liter exchange/day, an increase in mass transfer area coefficient, and 0.9 liters ultrafiltration/day. Urea, creatinine, and phosphate removal was 33.2 ± 4.1, 5.3 ± 0.5, and 6.2 ± 1.8 mmol, respectively, with the daytime system and 204 ± 28, 10.3 ± 2.4, and 11.4 ± 2.1 mmol, respectively, with the nighttime system. Time-averaged plasma clearances of urea, creatinine and phosphate were 9.6 ± 1.1, 9.6 ± 1.7, and 7.0 ± 0.9 mL/min, respectively, with the nighttime system and 10.8 ± 1.1, 13.4 ± 1.8, and 9.7 ± 1.6 mL/min, respectively, with the daytime and nighttime system. SAPD treatment may improve removal of uremic toxins compared with conventional peritoneal dialysis, provided that peritoneal mass transport will increase