20 research outputs found
Assessing variation in maize grain nitrogen concentration and its implications for estimating nitrogen balance in the US North Central region
Accurate estimation of nitrogen (N) balance (a measure of potential N losses) in producer fields requires information on grain N concentration (GNC) to estimate grain-N removal, which is rarely measured by producers. The objectives of this study were to (i) examine the degree to which variation in GNC can affect estimation of grain-N removal, (ii) identify major factors influencing GNC, and (iii) develop a predictive model to estimate GNC, analyzing the uncertainty in predicted grain-N removal at field and regional levels. We compiled GNC data from published literature and unpublished databases using explicit criteria to only include experiments that portray the environments and dominant management practices where maize is grown in the US North Central region, which accounts for one-third of global maize production. We assessed GNC variation using regression tree analysis and evaluated the ability of the resulting model to estimate grain-N removal relative to the current approach using a fixed GNC. Across all site-year-treatment cases, GNC averaged 1.15%, ranging from 0.76 to 1.66%. At any given grain yield, GNC varied substantially and resulted in large variation in estimated grain-N removal and N balance. However, compared with GNC, yield differences explained much more variability in grain-N removal. Our regression tree model accounted for 35% of the variation in GNC, and returned physiologically meaningful associations with mean air temperature and water balance in July (i.e., silking) and August (i.e., grain filling), and with N fertilizer rate. The predictive model has a slight advantage over the typical approach based on a fixed GNC for estimating grain-N removal for individual site-years (root mean square error: 17 versus 21 kg N ha−1, respectively). Estimates of grain-N removal with both approaches were more reliable when aggregated at climate-soil domain level relative to estimates for individual site-years
Assessing Variation in US Soybean Seed Composition (Protein and Oil)
Soybean [Glycine max (L.) Merr.] seed composition and yield are a function of genetics (G), environment (E), and management (M) practices, but contribution of each factor to seed composition and yield are not well understood. The goal of this synthesis-analysis was to identify the main effects of G, E, and M factors on seed composition (protein and oil concentration) and yield. The entire dataset (13,574 data points) consisted of 21 studies conducted across the United States (US) between 2002 and 2017 with varying treatments and all reporting seed yield and composition. Environment (E), defined as site-year, was the dominant factor accounting for more than 70% of the variation for both seed composition and yield. Of the crop management factors: (i) delayed planting date decreased oil concentration by 0.007 to 0.06% per delayed week (R2∼0.70) and a 0.01 to 0.04 Mg ha-1 decline in seed yield per week, mainly in northern latitudes (40–45 N); (ii) crop rotation (corn-soybean) resulted in an overall positive impact for both seed composition and yield (1.60 Mg ha-1 positive yield difference relative to continuous soybean); and (iii) other management practices such as no-till, seed treatment, foliar nutrient application, and fungicide showed mixed results. Fertilizer N application in lower quantities (10–50 kg N ha-1) increased both oil and protein concentration, but seed yield was improved with rates above 100 kg N ha-1. At southern latitudes (30–35 N), trends of reduction in oil and increases in protein concentrations with later maturity groups (MG, from 3 to 7) was found. Continuing coordinated research is critical to advance our understanding of G × E × M interactions
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Identification of a Rare Coding Variant in Complement 3 Associated with Age-related Macular Degeneration
Macular degeneration is a common cause of blindness in the elderly. To identify rare coding variants associated with a large increase in risk of age-related macular degeneration (AMD), we sequenced 2,335 cases and 789 controls in 10 candidate loci (57 genes). To increase power, we augmented our control set with ancestry-matched exome sequenced controls. An analysis of coding variation in 2,268 AMD cases and 2,268 ancestry matched controls revealed two large-effect rare variants; previously described R1210C in the CFH gene (fcase = 0.51%, fcontrol = 0.02%, OR = 23.11), and newly identified K155Q in the C3 gene (fcase = 1.06%, fcontrol = 0.39%, OR = 2.68). The variants suggest decreased inhibition of C3 by Factor H, resulting in increased activation of the alternative complement pathway, as a key component of disease biology
Assessing Variation in US Soybean Seed Composition (Protein and Oil)
Soybean [Glycine max (L.) Merr.] seed composition and yield are a function of genetics (G), environment (E), and management (M) practices, but contribution of each factor to seed composition and yield are not well understood. The goal of this synthesis-analysis was to identify the main effects of G, E, and M factors on seed composition (protein and oil concentration) and yield. The entire dataset (13,574 data points) consisted of 21 studies conducted across the United States (US) between 2002 and 2017 with varying treatments and all reporting seed yield and composition. Environment (E), defined as site-year, was the dominant factor accounting for more than 70% of the variation for both seed composition and yield. Of the crop management factors: (i) delayed planting date decreased oil concentration by 0.007 to 0.06% per delayed week (R2~0.70) and a 0.01 to 0.04 Mg ha-1 decline in seed yield per week, mainly in northern latitudes (40–45 N); (ii) crop rotation (corn-soybean) resulted in an overall positive impact for both seed composition and yield (1.60 Mg ha-1 positive yield difference relative to continuous soybean); and (iii) other management practices such as no-till, seed treatment, foliar nutrient application, and fungicide showed mixed results. Fertilizer N application in lower quantities (10–50 kg N ha-1) increased both oil and protein concentration, but seed yield was improved with rates above 100 kg N ha-1. At southern latitudes (30–35 N), trends of reduction in oil and increases in protein concentrations with later maturity groups (MG, from 3 to 7) was found. Continuing coordinated research is critical to advance our understanding of G x E x M interactions
Complement Activation Induces Neutrophil Adhesion and Neutrophil-Platelet Aggregate Formation on Vascular Endothelial Cells
Atypical hemolytic uremic syndrome is a thrombotic microangiopathy, which is linked to hereditary or autoimmune defects in complement activators or regulators present in blood and on vascular endothelial cells. Acute thrombotic microangiopathy episodes are typically preceded by infections, which by themselves would not be expected to manifest HUS. Thus, it is possible that the host immune response contributes to the precipitation of aHUS. However, the mechanisms involved are not fully understood. We hypothesized that neutrophils trigger aHUS via initiating platelet aggregate formation on complement-activated endothelial cells.
Methods: We investigated neutrophil adhesion to complement-activated endothelial cells under static and flow conditions in vitro and ex vivo.
Results: Our results show that complement activation on endothelial cells promotes neutrophil adhesion, which is significantly reduced when the complement terminal pathway is blocked. When neutrophils and platelets are perfused simultaneously, neutrophils adhering to endothelial cells also induce the formation of platelet-neutrophil aggregates on these cells. Sera from patients with aHUS recapitulated these results.
Discussion: Therefore, our findings of (i) neutrophils adhering to complement-activated endothelial cells, (ii) the formation of neutrophil-platelet aggregates on endothelial cells, and (iii) the ability of aHUS serum to induce similar effects identify a possible role for neutrophils in aHUS manifestation
Assessing variation in maize grain nitrogen concentration and its implications for estimating nitrogen balance in the US North Central region
Accurate estimation of nitrogen (N) balance (a measure of potential N losses) in producer fields requires information on grain N concentration (GNC) to estimate grain-N removal, which is rarely measured by producers. The objectives of this study were to (i) examine the degree to which variation in GNC can affect estimation of grain-N removal, (ii) identify major factors influencing GNC, and (iii) develop a predictive model to estimate GNC, analyzing the uncertainty in predicted grain-N removal at field and regional levels. We compiled GNC data from published literature and unpublished databases using explicit criteria to only include experiments that portray the environments and dominant management practices where maize is grown in the US North Central region, which accounts for one-third of global maize production. We assessed GNC variation using regression tree analysis and evaluated the ability of the resulting model to estimate grain-N removal relative to the current approach using a fixed GNC. Across all site-year-treatment cases, GNC averaged 1.15%, ranging from 0.76 to 1.66%. At any given grain yield, GNC varied substantially and resulted in large variation in estimated grain-N removal and N balance. However, compared with GNC, yield differences explained much more variability in grain-N removal. Our regression tree model accounted for 35% of the variation in GNC, and returned physiologically meaningful associations with mean air temperature and water balance in July (i.e., silking) and August (i.e., grain filling), and with N fertilizer rate. The predictive model has a slight advantage over the typical approach based on a fixed GNC for estimating grain-N removal for individual site-years (root mean square error: 17 versus 21 kg N ha−1, respectively). Estimates of grain-N removal with both approaches were more reliable when aggregated at climate-soil domain level relative to estimates for individual site-years.This is a manuscript of an article published as Tenorio, Fatima AM, Alison J. Eagle, Eileen L. McLellan, Kenneth G. Cassman, Reka Howard, Fred E. Below, David E. Clay et al. "Assessing variation in maize grain nitrogen concentration and its implications for estimating nitrogen balance in the US North Central region." Field Crops Research (2018). doi: 10.1016/j.fcr.2018.10.017. Posted with permission.</p
Assessing Variation in US Soybean Seed Composition (Protein and Oil)
Soybean [Glycine max (L.) Merr.] seed composition and yield are a function of genetics (G), environment (E), and management (M) practices, but contribution of each factor to seed composition and yield are not well understood. The goal of this synthesis-analysis was to identify the main effects of G, E, and M factors on seed composition (protein and oil concentration) and yield. The entire dataset (13,574 data points) consisted of 21 studies conducted across the United States (US) between 2002 and 2017 with varying treatments and all reporting seed yield and composition. Environment (E), defined as site-year, was the dominant factor accounting for more than 70% of the variation for both seed composition and yield. Of the crop management factors: (i) delayed planting date decreased oil concentration by 0.007 to 0.06% per delayed week (R2∼0.70) and a 0.01 to 0.04 Mg ha-1 decline in seed yield per week, mainly in northern latitudes (40–45 N); (ii) crop rotation (corn-soybean) resulted in an overall positive impact for both seed composition and yield (1.60 Mg ha-1 positive yield difference relative to continuous soybean); and (iii) other management practices such as no-till, seed treatment, foliar nutrient application, and fungicide showed mixed results. Fertilizer N application in lower quantities (10–50 kg N ha-1) increased both oil and protein concentration, but seed yield was improved with rates above 100 kg N ha-1. At southern latitudes (30–35 N), trends of reduction in oil and increases in protein concentrations with later maturity groups (MG, from 3 to 7) was found. Continuing coordinated research is critical to advance our understanding of G × E × M interactions.This article is published as Assefa, Yared, Larry C. Purcell, Montse Salmeron, Seth Naeve, Shaun N. Casteel, Péter Kovács, Sotirios Archontoulis et al. "Assessing variation in us soybean seed composition (protein and oil)." Frontiers in plant science 10 (2019): 298. doi: 10.3389/fpls.2019.00298.</p
Assessing Variation in US Soybean Seed Composition (Protein and Oil)
Soybean [Glycine max (L.) Merr.] seed composition and yield are a function of genetics (G), environment (E), and management (M) practices, but contribution of each factor to seed composition and yield are not well understood. The goal of this synthesis-analysis was to identify the main effects of G, E, and M factors on seed composition (protein and oil concentration) and yield. The entire dataset (13,574 data points) consisted of 21 studies conducted across the United States (US) between 2002 and 2017 with varying treatments and all reporting seed yield and composition. Environment (E), defined as site-year, was the dominant factor accounting for more than 70% of the variation for both seed composition and yield. Of the crop management factors: (i) delayed planting date decreased oil concentration by 0.007 to 0.06% per delayed week (R2∼0.70) and a 0.01 to 0.04 Mg ha-1 decline in seed yield per week, mainly in northern latitudes (40–45 N); (ii) crop rotation (corn-soybean) resulted in an overall positive impact for both seed composition and yield (1.60 Mg ha-1 positive yield difference relative to continuous soybean); and (iii) other management practices such as no-till, seed treatment, foliar nutrient application, and fungicide showed mixed results. Fertilizer N application in lower quantities (10–50 kg N ha-1) increased both oil and protein concentration, but seed yield was improved with rates above 100 kg N ha-1. At southern latitudes (30–35 N), trends of reduction in oil and increases in protein concentrations with later maturity groups (MG, from 3 to 7) was found. Continuing coordinated research is critical to advance our understanding of G × E × M interactions