The influence of survivin shRNA on the cell cycle and the invasion of SW480 cells of colorectal carcinoma

<p>Abstract</p> <p>Background</p> <p>The objective was to understand the influence of Survivin plasmid with short hairpin RNA (shRNA) on the cell cycle, invasion, and the silencing effect of Survivin gene in the SW480 cell of colorectal carcinoma.</p> <p>Methods</p> <p>A eukaryotic expression vector, PGCH1/Survivin shRNA, a segment sequence of Survivin as target, was created and transfected into colorectal carcinoma cell line SW480 by the non-lipid method. The influence on the Survivin protein was analyzed by Western blotting, while the cell cycle, cell apoptosis were analyzed by flow cytometry, and invasion of the cell was analyzed by Transwell's chamber method.</p> <p>Results</p> <p>After the transfection of PGCH1/Survivin shRNA, the expression of Survivin protein in SW480 cells was dramatically decreased by 60.68%, in which the cells were stopped at G2/M phase, even though no apoptosis was detected. The number of transmembranous cells of the experimental group, negative control group, and blank control group were 14.46 ± 2.11, 25.12 ± 8.37, and 25.86 ± 7.45, respectively (P <<it>0.05</it>).</p> <p>Conclusion</p> <p>Survivin shRNA could significantly reduce the expression of Survivin protein and invasion of SW480 cells. Changes in cell cycle were observed, but no apoptosis was induced.</p

A 0.35 THz Extended Interaction Oscillator based on Overmoded and Bi-Periodic Structure

An improved topology of Extended interaction oscillators (EIO) is presented with enhanced efficiency at 0.35 THz. A bi-periodic ladder-type structure is used for optimizing the performance of the TM 31−2π mode operation, based on bi-periodic interaction gaps composed of staggered short and long slots. The bi-periodic interaction mechanism permits to operate the circuit with a standing-wave field between the π and 2π modes, thus providing the potential to combine the advantages of both π and 2π modes in terms of both coupling and output performance. The resulting transverse TM 31 operating mechanism exhibits a good agreement with the bi-periodic structure in terms of coupling performance, with the short slot length positively correlated with the value of effective characteristic impedance M2R/Q . The circuit demonstrates attractive coupling and output characteristics by optimization of M2R/Q and Qe . A state-of-the-art value M2R/Q of 59.69 Ω and a moderate Qe of 2523.52 are achieved for a proposed eight-period ladder-type EIO. An RF power of 540 W is obtained with a 42.5 kV, 0.6 A sheet beam, with 2.1% electronic efficiency at 0.35 THz

Retrospective analysis of 217 fatal intoxication autopsy cases from 2009 to 2021: temporal trends in fatal intoxication at Tongji center for medicolegal expertise, Hubei, China

This retrospective analysis of fatal intoxication case autopsies was performed at Tongji Center for Medicolegal Expertise in Hubei (TCMEH) from 2009 to 2021 to obtain up-to-date information on intoxication cases. The objective was to describe important data about evolving patterns in intoxication occurrences, enhance public safety policies, and assist forensic examiners and police in more efficient handling of such cases. Analyses based on sex, age, topical exposure routes, toxic agents, and mode of death were performed using 217 records of intoxication cases collected from TCMEH as a sample, and the results were compared with reports previously published (from 1999 to 2008) from this institution. Deaths from intoxications occurred at a higher rate in males than in females and were most common among individuals aged 30–39 years. The most frequent method of exposure was oral ingestion. The causative agents of deadly intoxications have changed when compared to the data from the previous 10 years. For instance, deaths from amphetamine overdoses are becoming more prevalent gradually, whereas deaths due to carbon monoxide and rodenticide intoxication have declined dramatically. In 72 cases, pesticides continued to be the most frequent intoxication cause. A total of 60.4% of the deaths were accidental exposure. Men died from accidents at a higher rate than women, although women were more likely to commit suicide. Particular focus is needed on the use of succinylcholine, cyanide, and paraquat in homicides

Exploring the use of graphene lubricant and TiO2 nanolubricants in micro deep drawing of stainless steel SUS301

This study investigates the effects of different lubrication conditions on drawing force and microcup formation during micro deep drawing (MDD). Results show that graphene lubricant, in combination with TiO2 nanolubricants, has the potential to reduce friction during MDD. The peak drawing force was reduced by 15.39% when both lubricants were used together, while the use of TiO2 nanolubricant and 10.0 mg/ml graphene lubricant reduced it by 6.03% and 14.52%, respectively. The study also reveals that lubricants reduce wrinkling during the formation of microcups by minimising energy consumption during the primary formation. However, the combination of TiO2 nanolubricant and graphene lubricant can cause inhomogeneous formation on the upper part of the blank, leading to more apparent wrinkling. Overall, the study highlights the potential of TiO2 nanolubricant and graphene lubricant in reducing friction and improving microcup formation during MDD.Open Access funding enabled and organized by CAUL and its Member Institutions. This research received funding from the Australian Research Council (ARC) through the project designated as DP190100738

Proses Produksi Dan Pengemasan Yoghurtdicv. Cita Nasional Salatiga

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An efficient access to the synthesis of novel 12-phenylbenzo[6,7]oxepino[3,4-b]quinolin-13(6H)-one derivatives

An efficient access to the tetracyclic-fused quinoline systems, 12-phenylbenzo[6,7]oxepino[3,4-b]quinolin-13(6H)-one derivatives 4a–l, is described, involving the intramolecular Friedel–Crafts acylation reaction of 2-(phenoxymethyl)-4-phenylquinoline-3-carboxylic acid derivatives 3a–l aided by the treatment with PPA (polyphosphoric acid) or Eaton’s reagent. The required starting compound (2) was obtained by Friedländer reaction of 2-aminobenzophenone (1) with 4-chloroethylacetoacetate by using CAN (cerium ammonium nitrate, 10 mol %) as catalyst at room temperature. The substrates 3a–l were prepared through one-pot reaction of ethyl 2-(chloromethyl)-4-phenylquinoline-3-carboxylate (2) and substituted phenols. Our developed strategy, involving a three-step route, offers easy access to tetracyclic-fused quinoline systems in short reaction times, and the products are obtained in moderate to good yields

Convolutional Neural Network-Based Travel Mode Recognition Based on Multiple Smartphone Sensors

Nowadays, large-scale human mobility has led to increasingly severe traffic congestion in cities, how to accurately identify people’s travel mode has become particularly important for urban traffic planning and management. However, traditional methods are based on telephone interviews or questionnaires, which makes it difficult to obtain accurate and effective data. Nowadays, numbers of smartphones are equipped with various sensors, including accelerometers, gyroscopes, and GPS, providing a novel social sensing data source to detect people’s travel modes. The fusion of multiple sensor data is a promising way for travel mode detection. However, how to use these sensor data to accurately detect travel mode is still a challenging task. In this paper, we presented a light-weight method for travel mode detection based on four types of smartphone sensor data collected from an accelerometer, gyroscope, magnetometer, and barometer, and a prototype application was developed. Then, a novel convolutional neural network (CNN) was designed to identify five representative travel modes (walk, bicycle, bus, car, and metro). We compared the overall performance of the proposed method via different hyperparameters, and the experimental results show that the F value of the proposed method reaches 97%, which verified the effectiveness of the proposed method for travel mode classification

The Rapidly Evolving Fudu Estuary Sandbar Lagoon Landform on the East Coast of the Bohai Sea: Recent Changes and Mechanism

The Fudu Estuary Sandbar Lagoon is one of the most representative sandbar-lagoon landforms in China, and has undergone drastic evolution in recent years, accompanied by increased coastal engineering activities. The evolution process and its control factors are studied through remote sensing interpretation and coastal sediment transport calculations. During 2010–2021, the sandbar quickly extended at an average speed of 49.5 m/a, but the annual growth has shown a decreasing trend in both area and width, and the shoreline has retreated by 25–45 m. The recent changes are the result of the combined action of natural conditions and human activities. Coastal sediment transport from west to east under the action of W-oriented waves is the natural cause of extension. An estuary dam and artificial island block the sediment transport path, and the material for the new growth of the sandbar comes from the erosion of its west side, which has directly caused the retreat and narrowing of the sandbar. The reduction in sediments from the river further aggravates the shrinkage. It is predicted that the sandbar will continue its eastward extension to connect with the coast in about 2–3 years. The erosion status is unlikely to change before the sediment supply is restored. Measures such as dismantling the estuary dam are recommended