Identification and comparative analysis of the CIPK gene family and characterization of the cold stress response in the woody plant Prunus mume

Prunus mume is an important ornamental woody plant that grows in tropical and subtropical regions. Freezing stress can adversely impact plant productivity and limit the expansion of geographical locations. Understanding cold-responsive genes could potentially bring about the development of new ways to enhance plant freezing tolerance. Members of the serine/threonine protein kinase (CIPK) gene family play important roles in abiotic stress. However, the function of CIPK genes in P. mume remains poorly defined. A total of 16 CIPK genes were first identified in P. mume. A systematic phylogenetic analysis was conducted in which 253 CIPK genes from 12 species were divided into three groups. Furthermore, we analysed the chromosomal locations, molecular structures, motifs and domains of CIPK genes in P. mume. All of the CIPK sequences had NAF domains and promoter regions containing cis-acting regulatory elements of the related stress response. Three PmCIPK genes were identified as Pmu-miR172/167-targeted sites. Transcriptome data showed that most PmCIPK genes presented tissue-specific and time-specific expression profiles. Nine genes were highly expressed in flower buds in December and January, and 12 genes were up-regulated in stems in winter. The expression levels of 12 PmCIPK genes were up-regulated during cold stress treatment confirmed by qRT-PCR. Our study improves understanding of the role of the PmCIPK gene family in the low temperature response in woody plants and provides key candidate genes and a theoretical basis for cold resistance molecular-assisted breeding technology in P. mume

Influence of Acid Etching on Wettability of Ion-exchanged Aluminosilicate Float Glass

The influence of acid etching time on wettability of ion-exchanged aluminosilicate float glass was investigated. The contact angle, roughness and surface composition were measured. The results show that the contact angle increases to a maximum value in the first 7 min and then decreases with the corrosion time. The main reason that cause the change of the contact angle is the change of surface roughness and the content of fluorine atom. The contact angle on the tin side is always larger than that on the air side which is caused by the tin ions on the tin side

The influence of different cutting parameters on the glass edge quality

The influence of different cutting parameters on the glass edge quality was investigated, including the cutter material, the sharpening angle of the cutting roll, the cutting speed and the load applied to the roll. The results show that there are less defects on the edge of the glass cut by diamond cutter. There is no obvious influence of cutting speed on the glass edge quality. The cutter with a smaller sharpening angle is more applicable for the cutting of thin glass, and the thick glass is more suitable to use a bigger sharpening angle cutter. Higher cutting load is helpful for the breaking of the glass along the cutting line. However, it may cause more defects on the edge and the surface of the glass