Phthiriasis palpebrarum misdiagnosed as allergic blepharoconjunctivitis in a 6-year-old girl

Phthiriasis palpebrarum is an infestation of the eyelashes caused by the louse Pthirus pubis (Linnaeus, 1758). We report a case of phthiriasis  palpebrarum in a 6.year.old girl, which was initially misdiagnosed as allergicblepharoconjunctivitis. Parasites and their nits were found adhering to the eyelashes and eyelids of her right eye as well as scalp hairs. No  abnormality was found in the left eye. The histopathology exam revealed the presence of adults and eggs of Pthirus pubis. We mechanically removed all the eyelashes of the right eye at their base, with lice and nits. The scalp was shaved and washed with phenothrin shampoo. No recurrence was found during 3 months of follow.up. Removal of the eyelashes, cutting of scalp hairs, and phenothrin shampoo may be effective in treating  phthiriasis palpebrarum. In cases of blepharoconjunctivitis, eyelids and eyelashes should be carefully examined by slit lamp to avoid misdiagnosis.Key words: Blepharoconjunctivitis, crab louse, eyelashes, nits, pthirus pubis, phthiriasis palpebraru

In situ loading and delivery of short single- And double-stranded dna by supramolecular organic frameworks

Short DNA represents an important class of biomacromolecules that are widely applied in gene therapy, editing, and modulation. However, the development of simple and reliable methods for their intracellular delivery remains a challenge. Herein, we describe that seven water-soluble, homogeneous supramolecular organic frameworks (SOFs) with a well-defined pore size and high stability in water that can accomplish in situ inclusion of single-stranded (ss) and double-stranded (ds) DNA (21, 23, and 58 nt) and effective intracellular delivery (including two noncancerous and six cancerous cell lines). Fluorescence quenching experiments for single and double endlabeled ss- and ds-DNA support that the DNA sequences can be completely enveloped by the SOFs. Confocal laser scanning microscopy and flow cytometry reveal that five of the SOFs exhibit excellent delivery efficiencies that, in most of the studied cases, outperform the commercial standard Lipo2000, even at low SOF-nucleic acid ratios. In addition to high delivery efficiencies, the watersoluble, self-assembled SOF carriers have a variety of advantages, including convenient preparation, high stability, and in situ DNA inclusion, which are all critical for practical applications in nucleic acid delivery

Fabrication of a Micro-Needle Sensor Based on Copper Microspheres and Polyaniline Film for Nitrate Determination in Coastal River Waters

In this work, a new micro-needle sensor based on copper microspheres (CuMSs) and polyaniline (PANI) film was fabricated through electrodeposition method and used for the voltammetric determination of nitrate (NO3-) in coastal river samples. The PANI film functions as the conductive polymer and adhesion agent to immobilize the CuMSs which has good electrocatalytic property for the reduction of NO3-. The CuMSs could be more easily deposited on the micro-needle electrode (MNE) surface with PANI film as adhesion agent. The physical and electrochemical properties of the as-prepared micro-needle electrode were characterized by different techniques. CuMSs with diameter of about 0.5 to 1 mu m were decorated on the PANI film modified micro-needle electrode. The unique structure of micro-needle electrode and excellent properties of CuMSs and PANI film make the micro-needle sensor possesses the advantages of larger specific surface and high electrocatalytic property towards the reduction of NO3-. The micro-needle electrode based on CuMSs and PANI film shows a linear response to NO3- in the concentrations ranging from 0.02 to 6 mM (R-2 = 0.995) with the detection limit of 8 mu M. More importantly, the determination of NO3- in coastal river water samples was achieved with the micro-needle electrode with satisfactory results. (C) The Author(s) 2019. Published by ECS

Cyclin‐dependent kinase 4 inhibits the translational repressor 4E‐BP1 to promote cap‐dependent translation during mitosis–G1 transition

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/154953/1/feb213721_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154953/2/feb213721.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154953/3/feb213721-sup-0001-FigS1-S3.pd

Reduced expression of AMPK-β1 during tumor progression enhances the oncogenic capacity of advanced ovarian cancer

AMP-activated protein kinase (AMPK) is a key energy sensor that is involved in regulating cell metabolism. Our previous study revealed that the subunits of the heterotimeric AMPK enzyme are diversely expressed during ovarian cancer progression. However, the impact of the variable expression of these AMPK subunits in ovarian cancer oncogenesis remains obscure. Here, we provide evidence to show that reduced expression of the AMPK-beta1 subunit during tumor progression is associated with the increased oncogenic capacity of advanced ovarian cancer cells. Immunohistochemical analysis revealed that AMPK-beta1 levels were reduced in advanced-stage (P = 0.008), high-grade (P = 0.013) and metastatic ovarian cancers (P = 0.008). Intriguingly, down-regulation of AMPK-beta1 was progressively reduced from tumor stages 1 to 3 of ovarian cancer. Functionally, enforced expression of AMPK-beta1 inhibited ovarian-cancer-cell proliferation, anchorage-independent cell growth, cell migration and invasion. Conversely, depletion of AMPK-beta1 by siRNA enhanced the oncogenic capacities of ovarian cancer cells, suggesting that the loss of AMPK-beta1 favors the aggressiveness of ovarian cancer. Mechanistically, enforced expression of AMPK-beta1 increased AMPK activity, which, in turn, induced cell-cycle arrest via inhibition of AKT/ERK signaling activity as well as impaired cell migration/invasion through the suppression of JNK signaling in ovarian cancer cells. Taken together, these findings suggest that the reduced expression of AMPK-beta1 confers lower AMPK activity, which enhances the oncogenic capacity of advanced-stage ovarian cancer.published_or_final_versio

Unravelling the fast photocyclisation reaction mechanism(s) of 2-benzoylpyridine in aqueous solvent by time-resolved spectroscopy

Monday Poster Session: Time Resolved Raman (MP09) - Poster Number: 154A combined femtosecond transient absorption (fs-TA) and nanosecond time-resolved Resonance Raman (ns-TR3) spectroscopic investigation of the photoreaction of 2-benzoylpyridine in acetonitrile, neutral, basic and acidic aqueous solvents is reported. Some new species generated in the aqueous solvents observed for the first time and characterised by time-resolved vibrational spectroscopy in conjunction with results from DFT calculations. The detailed fast photocyclisation mechanism(s) of 2-benzoylpyridine in aqueous solvents after UV laser photolysis is obtained.postprintThe 22nd International Conference on Raman Spectroscopy (ICORS 2010), Boston, MA., 8-13 August 2010

Influence of transport and ocean ice extent on biogenic aerosol sulfur in the Arctic atmosphere

The recent decline in sea ice cover in the Arctic Ocean could affect the regional radiative forcing via changes in sea ice-atmosphere exchange of dimethyl sulfide (DMS) and biogenic aerosols formed from its atmospheric oxidation, such as methanesulfonic acid (MSA). This study examines relationships between changes in total sea ice extent north of 70 degrees N and atmospheric MSA measurement at Alert, Nunavut, during 1980-2009; at Barrow, Alaska, during 1997-2008; and at Ny-Alesund, Svalbard, for 1991-2004. During the 1980-1989 and 1990-1997 periods, summer (July-August) and June MSA concentrations at Alert decreased. In general, MSA concentrations increased at all locations since 2000 with respect to 1990 values, specifically during June and summer at Alert and in summer at Barrow and Ny-Alesund. Our results show variability in MSA at all sites is related to changes in the source strengths of DMS, possibly linked to changes in sea ice extent as well as to changes in atmospheric transport patterns. Since 2000, a late spring increase in atmospheric MSA at the three sites coincides with the northward migration of the marginal ice edge zone where high DMS emissions from ocean to atmosphere have previously been reported. Significant negative correlations are found between sea ice extent and MSA concentrations at the three sites during the spring and June. These results suggest that a decrease in seasonal ice cover influencing other mechanisms of DMS production could lead to higher atmospheric MSA concentrations

En-situ EXAFS investigation of zeolite supported Pt electrocatalyst structure

Experimental investigation was carried out for Pt electrochemical performance and Pt particle size using 1.5 wt% and 5 wt% Pt loading on zeolite electrocatalysts made by Pt(NH3)4(NO3)2 or Pt(NH3)4(NO3)2/NH4NO3 salt with ion exchanged method and calcined at 350 oC and reduced at 400 oC or direct reduced at 400 oC, respectively. Cyclic voltammetry measurement indicated that the hydrogen energy binding level on Pt surfaces is higher for electrocatalyst under direct reduction process than those made by calcination and reduction process. The extended X-ray adsorption fine structure measurement revealed that Pt size for electrocatalyst made by calcination and reduction method is smaller than those made by direct reduced method. Furthermore, Pt size for electrocatalysts with 1.5 wt% Pt loading on zeolite is smaller compared to those with 5 wt% Pt loading electrocatalysts. Aforementioned electrochemical performance of Pt zeolite electrocatalysts has depicted by a hypothesis of hydrogen spillover and surface conductance pathway