Crystallization and preliminary X-ray analysis of the sporulation factor SpoIIAA in its native and phosphorylated forms

Sporulation in Bacillus begins with an asymmetric cell division producing two progeny with identical chromosomes but different developmental fates. As such, it is a simple example of cellular differentiation. The establishment of cell type is controlled by a series of alternate RNA polymerase sigma subunits. The first compartment-specific sigma factor is sigma (F), whose activity is controlled by SpoIIAB, an anti-sigma factor, and SpoIIAA, an anti-sigma factor antagonist which is phosphorylated by the kinase activity of SpoIIAB. Here, the preliminary crystallographic analysis of SpoIIAA and phosphorylated SpoIIAA from B. sphaericus in forms suitable for high-resolution structure determination are reported

The Cosmic Microwave Background and the Ionization History of the Universe

Details of how the primordial plasma recombined and how the universe later reionized are currently somewhat uncertain. This uncertainty can restrict the accuracy of cosmological parameter measurements from the Cosmic Microwave Background (CMB). More positively, future CMB data can be used to constrain the ionization history using observations. We first discuss how current uncertainties in the recombination history impact parameter constraints, and show how suitable parameterizations can be used to obtain unbiased parameter estimates from future data. Some parameters can be constrained robustly, however there is clear motivation to model recombination more accurately with quantified errors. We then discuss constraints on the ionization fraction binned in redshift during reionization. Perfect CMB polarization data could in principle distinguish different histories that have the same optical depth. We discuss how well the Planck satellite may be able to constrain the ionization history, and show the currently very weak constraints from WMAP three-year data.Comment: Changes to match MNRAS accepted versio

A multi-spacecraft reanalysis of the atmosphere of Mars

We have conducted a nine-Mars Year (MY) consistent reanalysis of the martian atmosphere covering the period MY 24–32 and making use of data from three different spacecraft. Remotely-sensed measurements of temperature, dust opacity, water ice and ozone from NASA’s Mars Global Surveyor (MGS) and Mars Recconaisance Orbiter (MRO) and ESA’s Mars Express (MEx) were assimilated [1] into a single model simulation, sampled two-hourly over the whole period. This forms a large, regular reanalysis dataset that is being made publicly available as an output of the EU UPWARDS project. The same analysis technique, with an improved model and higher resolution will be conducted with ESA Trace Gas Orbiter (TGO) data as it becomes available

Trace gas assimilation of Mars orbiter observations

Ozone, water vapour and argon are minor constituents in the Martian atmosphere, observations of which can be of use in constraining atmospheric dynamical and physical processes. This is especially true in the winter season of each hemisphere, when the bulk of the main constituent in the atmosphere (CO2 ) condenses in the polar regions shifting the balance of atmospheric composition to a more trace gas rich air mass. Current Mars Global Circulation Models (MGCMs) are able to represent the photochemistry occuring in the atmosphere, with constraints being imposed by comparisons with observations. However, a long term comparison using data assimilation provides a more robust constraint on the model. We aim to provide a technique for trace gas data assimilation for the analysis of observations from current and future satellite missions (such as ExoMars) which observe the spatial and temporal distribution of trace gases on Mars

Automatic transponder

A method and apparatus for the automatic, remote measurement of the internal delay time of a transponder at the time of operation is provided. A small portion of the transmitted signal of the transponder is converted to the receive signal frequency of the transponder and supplied to the input of the transponder. The elapsed time between the receive signal locally generated and the receive signal causing the transmission of the transmitted signal is measured, said time being representative of or equal to the internal delay time of the transponder at the time of operation

Bacillus subtilis regulatory protein GerE

GerE is the latest-acting of a series of factors which regulate gene expression in the mother cell during sporulation in Bacillus. The gene encoding GerE has been cloned from B. subtilis and overexpressed in Escherichia coli. Purified GerE has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol as a precipitant. The small plate-like crystals belong to the monoclinic space group C2 and diffract beyond 2.2 Angstrom resolution with a synchrotron radiation X-ray source

Our Heritage, the Soil

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