An assessment of the dental resection prosthesis fixation

Department of Prosthodontics, Bukovinian State Medical University, Chernivtsi, Ukraine, Private dental clinic of Dr. R. Levandovskiy, Kolomiya, UkraineThe most effective ways of fixing the resection prostheses have been found and a perfected technique of the assessment of the carrying capacity of the prosthesis fixation for a particular patient has been worked out. The fixation of the resection prosthesis due to functional sticking and adhesion can be effective not only qualitatively but also quantitatively. It has been proved that depending on the particular clinical situation, by various methods of fixing the resection prosthesis along with the numerical evaluation of their effectiveness, the desired result to hold the prosthesis in the jaw for a long time can be achieved. The experimental studies to prove the effectiveness of resection prosthesis fixation are particularly important on condition of their correct doing and the results processing. The use of modern computer technologies (MIMICS, SolidWorks, etc.) makes it possible not only to determine the contact area of the prosthesis, but also to design the prosthesis and evaluate the properties of the tissues surrounding the prosthesis. It has been proved that in order to enhance the fixation of the complete dentures the adhesive creams should be applied as additional means. For practical health care in order to improve the fixation of resection prostheses of the upper jaw in the complete absence of teeth, it is necessary to recommend the mandatory use of adhesive creams by Superfiks, Superkorega and additional modeling of buccal cavities on the vestibular surface of the denture base

Реакції дикетону Куксона з галогенідами калію в середовищі поліфосфорної кислоти

Aim. To study the rearrangement of Cookson’s diketone by the action of potassium halides under conditions of polyphosphoric acid catalysis.Results and discussion. Chemical behaviour of Cookson’s diketone (CS-trishomocubane-8,11-dione) in the reactions with potassium halides (KCl, KBr, KI) in the polyphosphoric acid (PPA) medium have been studied. When treated with the KI/PPA mixture Cookson’s diketone undergoes reduction leading to tetracyclo[6.3.0.0.4,11.05,9]undecane-2,7-dione. The use of KBr instead of KI leads to formal addition of HBr to the cyclobutane ring of CS-trishomocubane-8,11-dione and gives 3-bromotetracyclo[6.3.0.04,11.05,9]undecane-2,7-dione. The general scheme of the cycle opening mechanism has been proposed. In the case of using the KCl/PPA mixture the reaction does not occur.Experimental part. The structure and composition of compounds were proved by the methods of 1H and 13C NMR-spectroscopy, and also X-ray diffraction analysis. Elemental analysis was performed for the compounds obtained.Conclusions. It has been shown that hydrohalic acids generated in situ under the reaction conditions do not induce the rearrangement of Cookson’s diketone to the D3-trishomocubane system. The cyclobutane ring opening and reduction take place instead. Received: 12.12.2019Revised: 10.01.2020Accepted: 27.02.2020Цель. Исследовать перегруппировку дикетонов Куксона под действием галогенидов калия в условиях катализа полифосфорной кислотой.Результаты и их обсуждение. Изучено химическое поведение дикетона Куксона (CS-трисгомокубан-8,11-диона) в реакции с галогенидами калия (KCl, KBr, KI) в среде полифосфорной кислоты (PPA). При обработке йодидом калия в среде полифосфорной кислоты дикетон Куксона вступает в реакцию восстановления, которая приводит к образованию тетрацикло[6.3.0.0.4,11.05,9]ундекан-2,7-диона. Использование KBr вместо KI приводит к формальному присоединению HBr по циклобутановому кольцу CS-трисгомокубан-8,11-диона, в результате чего образуется 3-бромтетрацикло [6.3.0.04,11.05,9]ундекан-2,7-дион. Предложена общая схема механизма раскрытия цикла. В случае использования системы KCl/PPA реакция не происходит.Экспериментальная часть. Структура и состав соединений были подтверждены методами 1Н и 13С ЯМР-спектроскопии, а также рентгеноструктурным анализом. Проведен элементный анализ полученных соединений.Выводы. Показано, что галогеноводородные кислоты, образующиеся in situ при смешивании галогенидов калия и полифосфорной кислоты, вместо перегруппировки дикетона Куксона в производные D3-трисгомокубана приводzт к восстановлению каркаса с раскрытием циклобутанового фрагмента. Received: 12.12.2019Revised: 10.01.2020Accepted: 27.02.2020Мета. Дослідити перегрупування дикетону Куксона під дією галогенідів калію в умовах каталізу поліфосфорною кислотою.Результати та їх обговорення. Вивчено хімічну поведінку дикетону Куксона (CS-трисгомокубан-8,11-діону) в реакції з галогенідами калію (KCl, KBr, KI) в середовищі поліфосфорної кислоти (PPA). При обробці йодидом калію в середовищі поліфосфорної кислоти дикетон Куксона вступає в реакцію відновлення, яка приводить до утворення тетрацикло[6.3.0.0.4,11.05,9]ундекан-2,7-діону. Використання KBr замість KI приводить до формального приєднання HBr до циклобутанового кільця CS-трисгомокубан-8,11-діону, в результаті чого утворюється 3-бромтетрацикло[6.3.0.04,11.05,9]ундекан-2,7-діон. Запропоновано загальну схему механізму розкриття циклу. У випадку використання системи KCl/PPA реакція не відбувається.Експериментальна частина. Структуру та склад сполук було доведено методами 1Н та 13С ЯМР-спектроскопії, а також рентгеноструктурним аналізом. Проведено елементний аналіз одержаних сполук.Висновки. Показано, що галогеноводневі кислоти, що утворюються in situ при змішуванні галогенідів калію та поліфосфорної кислоти, замість перегрупування дикетону Куксона в похідні D3-трисгомокубану спричиняють відновлення каркасу з розкриттям циклобутанового фрагмента. Received: 12.12.2019Revised: 10.01.2020Accepted: 27.02.202

Virtual screening, synthesis and biological evaluation of DNA intercalating antiviral agents

© 2017 Elsevier Ltd This paper describes computer-aided design of new anti-viral agents against Vaccinia virus (VACV) potentially acting as nucleic acid intercalators. Earlier obtained experimental data for DNA intercalation affinities and activities against Vesicular stomatitis virus (VSV) have been used to build, respectively, pharmacophore and QSAR models. These models were used for virtual screening of a database of 245 molecules generated around typical scaffolds of known DNA intercalators. This resulted in 12 hits which then were synthesized and tested for antiviral activity against VaV together with 43 compounds earlier studied against VSV. Two compounds displaying high antiviral activity against VaV and low cytotoxicity were selected for further antiviral activity investigations

Systemic availability of guanidinoacetate affects GABAA receptor function and seizure threshold in GAMT deficient mice.

Deficiency of guanidinoacetate methyltransferase (GAMT) causes creatine depletion and guanidinoacetate accumulation in brain with the latter deemed to be responsible for the severe seizure disorder seen in affected patients. We studied electrical brain activity and GABAA mediated mechanisms of B6J.Cg-Gamt(tm1Isb) mice. Electrocorticographic (ECoG) monitoring of pharmacological treatments with ornithine (5 % in drinking water for 5-18 days) and/or Picrotoxin (PTX) (a GABAA receptor antagonist) (1.5 mg/kg, I.P.) in Gamt(MUT) and Gamt(WT) groups [n = 3, mean age (SEM) = 6.9 (0.2) weeks]. Mice were fitted with two frontal and two parietal epidural electrodes under ketamine/xylazine anesthesia. Baseline and test recordings were performed for determination of seizure activity over a 2 h period. The ECoG baseline of Gamt(MUT) exhibited an abnormal monotonous cortical rhythm (7-8 Hz) with little variability during awake and sleep states compared to wild type recordings. Ornithine treatment and also PTX administration led to a relative normalization of the Gamt(MUT) ECoG phenotype. Gamt(WT) on PTX exhibited electro-behavioral seizures, whereas the Gamt(MUT) did not have PTX induced seizures at the same PTX dose. Gamt(MUT) treated with both ornithine and PTX did not show electro-behavioral seizures while ornithine elevated the PTX seizure threshold of Gamt(MUT) mice even further. These data demonstrate: (1) that there is expression of electrical seizure activity in this Gamt-deficient transgenic mouse strain, and (2) that the systemic availability of guanidinoacetate affects GABAA receptor function and seizure thresholds. These findings are directly and clinically relevant for patients with a creatine-deficiency syndrome due to genetic defects in GAMT and provide a rational basis for a combined ornithine/picrotoxin therapeutic intervention

Intermittent peripheral weakness as the presenting feature of pyruvate dehydrogenase deficiency.

Two unrelated children presenting with episodic isolated peripheral weakness were found to have pyruvate dehydrogenase (PDH) deficiency (OMIM 312170) due to previously undescribed mutations (Pro250Thr, Arg88Cys) in the gene for the E1alpha subunit (PDHA1). Taken in context with the literature, these patients suggest that acute weakness initially resembling Guillain-Barre syndrome is a potentially reversible and probably underdiagnosed manifestation of PDH deficiency and that peripheral nerve function should be evaluated in PDH-deficient patients

Virtual screening, synthesis and biological evaluation of DNA intercalating antiviral agents

© 2017 Elsevier Ltd This paper describes computer-aided design of new anti-viral agents against Vaccinia virus (VACV) potentially acting as nucleic acid intercalators. Earlier obtained experimental data for DNA intercalation affinities and activities against Vesicular stomatitis virus (VSV) have been used to build, respectively, pharmacophore and QSAR models. These models were used for virtual screening of a database of 245 molecules generated around typical scaffolds of known DNA intercalators. This resulted in 12 hits which then were synthesized and tested for antiviral activity against VaV together with 43 compounds earlier studied against VSV. Two compounds displaying high antiviral activity against VaV and low cytotoxicity were selected for further antiviral activity investigations

Virtual screening, synthesis and biological evaluation of DNA intercalating antiviral agents

© 2017 Elsevier Ltd This paper describes computer-aided design of new anti-viral agents against Vaccinia virus (VACV) potentially acting as nucleic acid intercalators. Earlier obtained experimental data for DNA intercalation affinities and activities against Vesicular stomatitis virus (VSV) have been used to build, respectively, pharmacophore and QSAR models. These models were used for virtual screening of a database of 245 molecules generated around typical scaffolds of known DNA intercalators. This resulted in 12 hits which then were synthesized and tested for antiviral activity against VaV together with 43 compounds earlier studied against VSV. Two compounds displaying high antiviral activity against VaV and low cytotoxicity were selected for further antiviral activity investigations

Virtual screening, synthesis and biological evaluation of DNA intercalating antiviral agents

© 2017 Elsevier Ltd This paper describes computer-aided design of new anti-viral agents against Vaccinia virus (VACV) potentially acting as nucleic acid intercalators. Earlier obtained experimental data for DNA intercalation affinities and activities against Vesicular stomatitis virus (VSV) have been used to build, respectively, pharmacophore and QSAR models. These models were used for virtual screening of a database of 245 molecules generated around typical scaffolds of known DNA intercalators. This resulted in 12 hits which then were synthesized and tested for antiviral activity against VaV together with 43 compounds earlier studied against VSV. Two compounds displaying high antiviral activity against VaV and low cytotoxicity were selected for further antiviral activity investigations