Les stratégies de contrôle de la qualité sanitaire dans les Organisations de Producteurs de tomates

Our paper deals with free-riding issues in product safety control at the collective marketing level. It focuses on the empirical findings of the exhaustive face to face survey that has been conducted in 2007 by the authors with the quality managers of the tomato growers'unions of France. Our survey was supported by the National Tomato Charter, a regrouping of most of the French tomato growers'unions. Its aim was to identify the differences in monitoring and enforcement practices at the collective marketing level and to search for any group size effect on such practices. A main finding of our paper is that, beyond public regulation and collective rules imposed by the National Tomato Charter, POs implement voluntary control rules for a better control of free riding behavior within the group. Such additional rules differ both in monitoring intensity (measured by the number of pesticide residue analysis performed voluntarily by the group) and in sanction level (applied in case of deviation to the collective rule). Differences in safety control are not only triggered by customers specific requirements but also by group size (measured by the number of growers within the group). Eventually, our paper draws some perspectives for a more in-depth analysis of free riding issues. First, it calls for paying more attention to factors such as intra-group heterogeneity and differences in customers requirements. Second, it underlines the need for a more extensive analysis which includes safety control practices at the production level and wonders whether such practices are a complement or a substitute of control practices at the marketing level. ...French Abstract : Ce texte aborde les problèmes d'action collective dans le contrôle de la qualité sanitaire des produits avant leur mise en marché par les Organisations de Producteurs. Il est une synthèse des résultats de l'enquête menée auprès des responsables qualité de l'ensemble des OP adhérant à la Charte Nationale Tomate sur leurs pratiques de contrôle de la qualité sanitaire des tomates. L'enquête a eu le soutien des responsables de la Charte Nationale Tomate, qui regroupe la quasi-totalité de la production organisée en France. Elle avait pour objectif d'identifier la diversité des pratiques de contrôle au sein des OP et de mesurer l'effet de la "taille de l'OP" sur les modalités de contrôle mises en place. L'enquête montre qu'au-delà des règles communes imposées par la réglementation et la Charte Nationale Tomate, les OP mettent en place des démarches de contrôle diversifiées qui se différencient à la fois par l'importance de la surveillance (mesurée en nombre d'analyses de résidus de pesticides) et par le niveau des sanctions appliquées en cas de dérogation à la règle adoptée. Elle explique enfin la diversité de ces démarches, non seulement par les exigences de la clientèle mais aussi par la taille du groupe (mesurée par le nombre de producteurs). Notre rapport se termine par une discussion sur les améliorations à apporter à ce premier travail. Des précisions sont à apporter tout d'abord sur l'hétérogénéité intra et inter groupes et sur les niveaux d'exigences de la clientèle. Un complément d'analyse est ensuite nécessaire pour mieux caractériser l'effort de contrôle en amont du produit, au niveau de la production et pour étudier la complémentarité ou la substituabilité des deux types de contrôle: contrôle sur les pratiques en production, contrôle sur le produit.PRODUCERS' UNIOS; FREE-RIDING; MONITORING; ENFORCEMENT; CONTROL; PESTICIDE RESIDUES; TOMATO; FRANCE; ORGANISATIONS DE PRODUCTEURS; PASSAGER CLANDESTIN; SANCTION; CONTROLE; RESIDUS DE PESTICIDES; TOMATE

Masitinib (AB1010), a Potent and Selective Tyrosine Kinase Inhibitor Targeting KIT

International audienceBackground: The stem cell factor receptor, KIT, is a target for the treatment of cancer, mastocytosis, and inflammatory diseases. Here, we characterise the in vitro and in vivo profiles of masitinib (AB1010), a novel phenylaminothiazole-type tyrosine kinase inhibitor that targets KIT. Methodology/Principal Findings: In vitro, masitinib had greater activity and selectivity against KIT than imatinib, inhibiting recombinant human wild-type KIT with an half inhibitory concentration (IC50) of 200 ± 40 nM and blocking stem cell factor-induced proliferation and KIT tyrosine phosphorylation with an IC50 of 150 ± 80 nM in Ba/F3 cells expressing human or mouse wild-type KIT. Masitinib also potently inhibited recombinant PDGFR and the intracellular kinase Lyn, and to a lesser extent, fibroblast growth factor receptor 3. In contrast, masitinib demonstrated weak inhibition of ABL and c-Fms and was inactive against a variety of other tyrosine and serine/threonine kinases. This highly selective nature of masitinib suggests that it will exhibit a better safety profile than other tyrosine kinase inhibitors; indeed, masitinib-induced cardiotoxicity or genotoxicity has not been observed in animal studies. Molecular modelling and kinetic analysis suggest a different mode of binding than imatinib, and masitinib more strongly inhibited degranulation, cytokine production, and bone marrow mast cell migration than imatinib. Furthermore, masitinib potently inhibited human and murine KIT with activating mutations in the juxtamembrane domain. In vivo, masitinib blocked tumour growth in mice with subcutaneous grafts of Ba/F3 cells expressing a juxtamembrane KIT mutant. Conclusions: Masitinib is a potent and selective tyrosine kinase inhibitor targeting KIT that is active, orally bioavailable in vivo, and has low toxicit

Depression in Patients with Mastocytosis: Prevalence, Features and Effects of Masitinib Therapy

Depression in patients with mastocytosis is often reported but its prevalence and characteristics are not precisely described. In addition, the impact of therapies targeting mast cells proliferation, differentiation and degranulation on psychic symptoms of depression have never been investigated. Our objective was to determine the prevalence and to describe features of depression in a large cohort of mastocytosis patients (n = 288) and to investigate the therapeutic impact of the protein kinase inhibitor masitinib in depression symptoms. The description of depression was based on the analysis of a database with Hamilton scores using Principal Component Analysis (PCA). Efficacy of masitinib therapy was evaluated using non parametric Wilcoxon test for paired data within a three months period (n = 35). Our results show that patients with indolent mastocytosis present an elevated prevalence of depression (64%). Depression was moderate in 56% but severe in 8% of cases. Core symptoms (such as psychic anxiety, depressed mood, work and interests) characterized depression in mastocytosis patients. Masitinib therapy was associated with significant improvement (67% of the cases) of overall depression, with 75% of recovery cases. Global Quality of Life slightly improved after masitinib therapy and did not predicted depression improvement. In conclusion, depression is very frequent in mastocytosis patients and masitinib therapy is associated with the reduction its psychic experiences. We conclude that depression in mastocytosis may originate from processes related to mast cells activation. Masitinib could therefore be a useful treatment for mastocytosis patients with depression and anxiety symptoms

Time-resolved single-crystal X-ray crystallography

In this chapter the development of time-resolved crystallography is traced from its beginnings more than 30 years ago. The importance of being able to “watch” chemical processes as they occur rather than just being limited to three-dimensional pictures of the reactant and final product is emphasised, and time-resolved crystallography provides the opportunity to bring the dimension of time into the crystallographic experiment. The technique has evolved in time with developments in technology: synchrotron radiation, cryoscopic techniques, tuneable lasers, increased computing power and vastly improved X-ray detectors. The shorter the lifetime of the species being studied, the more complex is the experiment. The chapter focusses on the results of solid-state reactions that are activated by light, since this process does not require the addition of a reagent to the crystalline material and the single-crystalline nature of the solid may be preserved. Because of this photoactivation, time-resolved crystallography is often described as “photocrystallography”. The initial photocrystallographic studies were carried out on molecular complexes that either underwent irreversible photoactivated processes where the conversion took hours or days. Structural snapshots were taken during the process. Materials that achieved a metastable state under photoactivation and the excited (metastable) state had a long enough lifetime for the data from the crystal to be collected and the structure solved. For systems with shorter lifetimes, the first time-resolved results were obtained for macromolecular structures, where pulsed lasers were used to pump up the short lifetime excited state species and their structures were probed by using synchronised X-ray pulses from a high-intensity source. Developments in molecular crystallography soon followed, initially with monochromatic X-ray radiation, and pump-probe techniques were used to establish the structures of photoactivated molecules with lifetimes in the micro- to millisecond range. For molecules with even shorter lifetimes in the sub-microsecond range, Laue diffraction methods (rather than using monochromatic radiation) were employed to speed up the data collections and reduce crystal damage. Future developments in time-resolved crystallography are likely to involve the use of XFELs to complete “single-shot” time-resolved diffraction studies that are already proving successful in the macromolecular crystallographic field.</p

Tunable photonic crystals controlled by spin-crossover material in Terahertz range

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Tunable photonic crystals controlled by spin-crossover material in Terahertz range

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Time-Domain Spectroscopy of Spin State Transition in Polymeric Spin Crossover compounds

International audienceWe have measured the evolution of the THz spectrum of polymeric iron(II) spin crossover compounds within the Low-Spin/High-Spin thermal hysteresis loop in the ~0.6-6 THz frequency range. This study enabled to evidence the large variations of both the refractive optical index and the absorption that occur during the spin state phase transition

High spin <-> low spin ultrafast excitation and relaxation of an isolated iron(II) complex

Picosecond and femtosecond time resolved pump-probe experiments make it possible to study both the low spin (LS) to high spin (HS) and high spin to low spin excitation and relaxation processes in the same isolated iron(II) complex. We demonstrate that both LS → HS and HS → LS can be recorded by changing the pump wavelength and occur on the same time scale

Local magnetism in granular iron/iron oxide nanostructures: a phase- and site- selective X-ray magnetic circular dichroism study

We present a study of the local magnetic properties of iron/iron oxide granular nanostructures by x-ray magnetic circular dichroism XMCD. Metallic iron -Fe nanoparticles, with average sizes ranging from 5 to 13 nm, are embedded in a nanocrystalline oxide matrix composed of both magnetite Fe3O4 and maghemite -Fe2O3. These granular samples were synthesized by cold compacting core-shell nanoparticles, in which a 2\u20133 nm-thick oxide layer surrounds the iron particles, synthesized by inert gas condensation. By exploiting the chemical selectivity and site sensitivity of XMCD, we were able to separate the magnetic contributions of the metallic core and of the two oxide phases present in the matrix and to study their behavior as a function of iron particle size and applied magnetic induction field. We detected the presence of a significant spin canting, predominantly affecting the octahedral sites of Fe in the oxide phase, and studied its dependence on the degree of structural disorder and applied magnetic induction field