Associação entre prática de atividade física com a saúde mental e a percepção da qualidade de vida em profissionais de enfermagem de Ribeirão Preto e região durante a pandemia da covid-19

Introduction: At the forefront of the COVID-19 pandemic are health professionals who carry out intense work, including nursing professionals, who represent a large number of health professionals working in health and hospital institutions. The high pressures in the work environment with expended workloads and journeys increased the demands due to the responsibility of their attributions. This context can negatively affect these professionals’ physical activity practices, mental health, and perceived quality of life. Objective: Analyze the association between physical activity practice, mental health, and perceived quality of life in nursing professionals from Ribeirao Preto, SP, during the COVID-19 pandemic. Methods: This cross-sectional study was performed with nursing professionals from the Ribeirao Preto and region area in 2021 through an online form on the Google Forms tool. Questions related to sociodemographic data, professional activity, questionnaires to measure physical activity levels and sedentary behavior (IPAQ - short version), quality of life perception (SF - 12v2), and mental health (DASS-21) were used. Regarding the statistical analysis, the Chi-square test was used to verify the association of two categorical variables; the Student’s T-test to compare two means, and the ANOVA to compare three means from unpaired samples. The significance level was 5%. Results: 125 professionals participated in the study, such as assistants, technicians, nurses, and coordinating nurses. Among them, 79.2% were women, 56.0% worked in the private sector, 34.4% were overweight, and 29.6% were obese. Professionals who practiced physical exercise or sport had a lower risk of depression (57.9% among non-practicing professionals, 47.9% among practicing one type of exercise, and 18.2%among practicing two or more types of exercise or sports, p = 0.04). In addition, professionals with moderate or high physical activity levels and who practiced physical exercises or sports had a better score in multiple domains and physical and mental quality of life components compared with low physical activity levels and not practicing physical exercises or sports, respectively. Conclusion: Nursing professionals who reported practicing more physical exercises or sports had lower mental health-related risks, and those who reported moderate or high physical activity levels had a better perception of quality of life.Introdução: Na linha de frente da pandemia da COVID-19 encontram-se os profissionais de saúde que realizam um intenso trabalho, incluindo os profissionais de enfermagem, que representam uma grande parcela entre os profissionais de saúde que atuam nas instituições de saúde e hospitalares. As altas pressões no ambiente de trabalho com elevadas cargas e jornadas aumentaram as demandas, devido à responsabilidade de suas atribuições. Este contexto pode trazer repercussões negativas para a prática da atividade física, saúde mental e percepção de qualidade de vida destes profissionais. Objetivo: Analisar a associação entre a prática de atividade física, saúde mental e a percepção da qualidade de vida em profissionais de enfermagem de Ribeirão Preto, SP durante a pandemia da COVID-19. Métodos: Trata-se de um estudo transversal realizado com profissionais de enfermagem de Ribeirão Preto e região em 2021, por meio de formulário online na ferramenta Google Forms. Foram realizadas perguntas relacionadas aos dados sociodemográficos, atividade profissional, questionários para medir nível de atividade física e comportamento sedentário (IPAQ – versão curta), percepção da qualidade de vida (SF – 12v2) e saúde mental (DASS-21). Sobre a análise estatística, utilizou-se o teste Qui-quadrado para verificar a associação de duas variáveis categóricas entre si; teste t de Student para comparar duas médias e a ANOVA para comparar três médias provenientes de amostras não pareadas. O nível de significância foi de 5%. Resultados: Participaram do estudo 125 profissionais de enfermagem, sendo auxiliares, técnicos, enfermeiros(as) e enfermeiros(as) coordenadores(as). Desses, 79,2% foram mulheres e 56% atuavam no setor privado, 34,4% estavam acima do peso e 29,6% com obesidade. Os profissionais que praticavam exercício físico ou esporte apresentaram menor risco de depressão (57,9% entre os que não praticavam, 47,9% entre os que praticavam um tipo e 18,2% entre os que praticavam dois ou mais tipos de exercício ou esporte, p = 0,04). Profissionais com moderado ou alto nível de atividade física e que praticavam exercícios físicos ou esportes, apresentaram melhor pontuação em diversos domínios e nos componentes físico e mental da qualidade de vida, quando comparados aos com baixo nível de atividade física e que não praticavam exercícios físicos ou esportes, respectivamente. Conclusão: Profissionais de enfermagem que relataram praticar mais exercícios físicos ou esporte apresentaram menor risco relacionado à saúde mental, e os que relataram nível moderado ou alto de atividade física apresentaram melhor percepção de qualidade de vida

Associação entre prática de atividade física com a saúde mental e a percepção da qualidade de vida em profissionais de enfermagem de Ribeirão Preto e região durante a pandemia da covid-19

Introdução: Na linha de frente da pandemia da COVID-19 encontram-se os profissionais de saúde que realizam um intenso trabalho, incluindo os profissionais de enfermagem, que representam uma grande parcela entre os profissionais de saúde que atuam nas instituições de saúde e hospitalares. As altas pressões no ambiente de trabalho com elevadas cargas e jornadas aumentaram as demandas, devido à responsabilidade de suas atribuições. Este contexto pode trazer repercussões negativas para a prática da atividade física, saúde mental e percepção de qualidade de vida destes profissionais. Objetivo: Analisar a associação entre a prática de atividade física, saúde mental e a percepção da qualidade de vida em profissionais de enfermagem de Ribeirão Preto, SP durante a pandemia da COVID-19. Métodos: Trata-se de um estudo transversal realizado com profissionais de enfermagem de Ribeirão Preto e região em 2021, por meio de formulário online na ferramenta Google Forms. Foram realizadas perguntas relacionadas aos dados sociodemográficos, atividade profissional, questionários para medir nível de atividade física e comportamento sedentário (IPAQ – versão curta), percepção da qualidade de vida (SF – 12v2) e saúde mental (DASS-21). Sobre a análise estatística, utilizou-se o teste Qui-quadrado para verificar a associação de duas variáveis categóricas entre si; teste t de Student para comparar duas médias e a ANOVA para comparar três médias provenientes de amostras não pareadas. O nível de significância foi de 5%. Resultados: Participaram do estudo 125 profissionais de enfermagem, sendo auxiliares, técnicos, enfermeiros(as) e enfermeiros(as) coordenadores(as). Desses, 79,2% foram mulheres e 56% atuavam no setor privado, 34,4% estavam acima do peso e 29,6% com obesidade. Os profissionais que praticavam exercício físico ou esporte apresentaram menor risco de depressão (57,9% entre os que não praticavam, 47,9% entre os que praticavam um tipo e 18,2% entre os que praticavam dois ou mais tipos de exercício ou esporte, p = 0,04). Profissionais com moderado ou alto nível de atividade física e que praticavam exercícios físicos ou esportes, apresentaram melhor pontuação em diversos domínios e nos componentes físico e mental da qualidade de vida, quando comparados aos com baixo nível de atividade física e que não praticavam exercícios físicos ou esportes, respectivamente. Conclusão: Profissionais de enfermagem que relataram praticar mais exercícios físicos ou esporte apresentaram menor risco relacionado à saúde mental, e os que relataram nível moderado ou alto de atividade física apresentaram melhor percepção de qualidade de vida

Incidence of severe critical events in paediatric anaesthesia (APRICOT): a prospective multicentre observational study in 261 hospitals in Europe

Background Little is known about the incidence of severe critical events in children undergoing general anaesthesia in Europe. We aimed to identify the incidence, nature, and outcome of severe critical events in children undergoing anaesthesia, and the associated potential risk factors. Methods The APRICOT study was a prospective observational multicentre cohort study of children from birth to 15 years of age undergoing elective or urgent anaesthesia for diagnostic or surgical procedures. Children were eligible for inclusion during a 2-week period determined prospectively by each centre. There were 261 participating centres across 33 European countries. The primary endpoint was the occurence of perioperative severe critical events requiring immediate intervention. A severe critical event was defined as the occurrence of respiratory, cardiac, allergic, or neurological complications requiring immediate intervention and that led (or could have led) to major disability or death. This study is registered with ClinicalTrials.gov, number NCT01878760. Findings Between April 1, 2014, and Jan 31, 2015, 31â127 anaesthetic procedures in 30â874 children with a mean age of 6·35 years (SD 4·50) were included. The incidence of perioperative severe critical events was 5·2% (95% CI 5·0â5·5) with an incidence of respiratory critical events of 3·1% (2·9â3·3). Cardiovascular instability occurred in 1·9% (1·7â2·1), with an immediate poor outcome in 5·4% (3·7â7·5) of these cases. The all-cause 30-day in-hospital mortality rate was 10 in 10â000. This was independent of type of anaesthesia. Age (relative risk 0·88, 95% CI 0·86â0·90; p<0·0001), medical history, and physical condition (1·60, 1·40â1·82; p<0·0001) were the major risk factors for a serious critical event. Multivariate analysis revealed evidence for the beneficial effect of years of experience of the most senior anaesthesia team member (0·99, 0·981â0·997; p<0·0048 for respiratory critical events, and 0·98, 0·97â0·99; p=0·0039 for cardiovascular critical events), rather than the type of health institution or providers. Interpretation This study highlights a relatively high rate of severe critical events during the anaesthesia management of children for surgical or diagnostic procedures in Europe, and a large variability in the practice of paediatric anaesthesia. These findings are substantial enough to warrant attention from national, regional, and specialist societies to target education of anaesthesiologists and their teams and implement strategies for quality improvement in paediatric anaesthesia. Funding European Society of Anaesthesiology

Incidence of severe critical events in paediatric anaesthesia (APRICOT): a prospective multicentre observational study in 261 hospitals in Europe

Background Little is known about the incidence of severe critical events in children undergoing general anaesthesia in Europe. We aimed to identify the incidence, nature, and outcome of severe critical events in children undergoing anaesthesia, and the associated potential risk factors. Methods The APRICOT study was a prospective observational multicentre cohort study of children from birth to 15 years of age undergoing elective or urgent anaesthesia for diagnostic or surgical procedures. Children were eligible for inclusion during a 2-week period determined prospectively by each centre. There were 261 participating centres across 33 European countries. The primary endpoint was the occurence of perioperative severe critical events requiring immediate intervention. A severe critical event was defined as the occurrence of respiratory, cardiac, allergic, or neurological complications requiring immediate intervention and that led (or could have led) to major disability or death. This study is registered with ClinicalTrials.gov, number NCT01878760. Findings Between April 1, 2014, and Jan 31, 2015, 31 127 anaesthetic procedures in 30 874 children with a mean age of 6.35 years (SD 4.50) were included. The incidence of perioperative severe critical events was 5.2% (95% CI 5.0-5.5) with an incidence of respiratory critical events of 3.1% (2.9-3.3). Cardiovascular instability occurred in 1.9% (1.7-2.1), with an immediate poor outcome in 5.4% (3.7-7.5) of these cases. The all-cause 30-day in-hospital mortality rate was 10 in 10 000. This was independent of type of anaesthesia. Age (relative risk 0.88, 95% CI 0.86-0.90; p<0.0001), medical history, and physical condition (1.60, 1.40-1.82; p<0.0001) were the major risk factors for a serious critical event. Multivariate analysis revealed evidence for the beneficial effect of years of experience of the most senior anaesthesia team member (0.99, 0.981-0.997; p<0.0048 for respiratory critical events, and 0.98, 0.97-0.99; p=0.0039 for cardiovascular critical events), rather than the type of health institution or providers. Interpretation This study highlights a relatively high rate of severe critical events during the anaesthesia management of children for surgical or diagnostic procedures in Europe, and a large variability in the practice of paediatric anaesthesia. These findings are substantial enough to warrant attention from national, regional, and specialist societies to target education of anaesthesiologists and their teams and implement strategies for quality improvement in paediatric anaesthesia

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field