Gaia TGAS search for Large Magellanic Cloud runaway supergiant stars:Candidate hypervelocity star discovery, and the nature of R71

We search for runaway stars in the Large Magellanic Cloud (LMC) by computing the space velocities of the visually brightest stars in the LMC that are included in the Gaia TGAS proper motion catalog. We compare with predictions from stellar dynamical models to obtain (peculiar) velocities relative to their local stellar environment. Two of the 31 stars have unusually high proper motions. Of the remaining 29 stars we find that most objects in this sample have velocities in very good agreement with model predictions of a circularly rotating disk model. Indeed the excellent fit to the model implies that the TGAS uncertainty estimates are likely overestimated. The fastest outliers in this subsample contain the LBV R71 and a few other well known emission line objects though in no case do we derive velocities consistent with fast (~100 km/s) runaways. Our results imply that R 71 in particular has a moderate deviation from the local stellar velocity field (40 km/s) lending support to the proposition that this object cannot have evolved as a normal single star since it lies too far from massive star forming complexes to have arrived at its current position during its lifetime. Our findings therefore strengthen the case for this LBV being the result of binary evolution. Of the two stars with unusually high proper motions we find that one, the isolated B1.5 Ia+ supergiant Sk-67 2 (HIP 22237), is a candidate hypervelocity star, the TGAS proper motion implying a very large peculiar transverse velocity (~360 km/s) directed radially away from the LMC centre. If confirmed, for example by Gaia Data Release 2, it would imply that this massive supergiant, on the periphery of the LMC, is leaving the galaxy where it will explode as a supernova.Comment: 7 pages, 5 figures, revised versio

A CNO Dichotomy among O2 Giant Spectra in the Magellanic Clouds

From a survey of the 3400 Å region in the earliest O-type spectra, we have found that two of the four O2 giants observed in the Large Magellanic Cloud have O IV lines there that are stronger than the N IV lines, while the other two have the opposite. A Small Magellanic Cloud counterpart also has N IV stronger than O IV. Inspection of the blue spectra of these stars shows that the former pair have weaker N lines in all ionization states (III, IV, and V) present as well as lines of C IV λ4658, while the latter three have stronger N lines and greater He/H. Space ultraviolet observations of two of the N-strong stars show N V wind profiles substantially stronger than those of C IV, while in the N-weak stars the C IV features are equal to or stronger than the N V. The N-strong stars are now reclassified as ON2 III(f*), newly defining that category. These characteristics strongly suggest a larger fraction of processed material in the atmospheres of the ON2 stars, which we confirm by modeling the optical spectra. In the context of current models, it is in turn implied that the ON2 stars are in a more advanced evolutionary state than the others, and/or that they had higher initial rotational velocities. The recent formulation of the effects of rotation on massive stellar evolution introduces an additional fundamental parameter, which the CNO abundances are in principle able to constrain. We present some illustrative comparisons with current Geneva evolutionary models for rotating massive stars. It is possible that these very hot, nitrogen-rich objects are products of homogeneous evolution. Our results will provide motivation for further physical modeling of the atmospheres and evolutionary histories of the most massive hot stars.Fil: Walborn, Nolan Revere. Space Telescope Science Institute; Estados UnidosFil: Morrell, Nidia Irene. Las campanas observatory; ChileFil: Howarth, Ian D.. University College London; Estados UnidosFil: Crowther, Paul A.. University of Sheffield; Reino UnidoFil: Lennon, Daniel J.. Isaac Newton Group of Telescopes; EspañaFil: Massey, Philip. Lowell Observatory; Estados UnidosFil: Arias, Julia Ines. Universidad Nacional de la Plata. Facultad de Ciencias Astronómicas y Geofísicas; Argentin

Reactive Chlorine Species Reversibly Inhibit DnaB Protein Splicing in Mycobacteria

Intervening proteins, or inteins, are mobile genetic elements that are translated within host polypeptides and removed at the protein level by splicing. In protein splicing, a self-mediated reaction removes the intein, leaving a peptide bond in place. While protein splicing can proceed in the absence of external cofactors, several examples of conditional protein splicing (CPS) have emerged. In CPS, the rate and accuracy of splicing are highly dependent on environmental conditions. Because the activity of the intein-containing host protein is compromised prior to splicing and inteins are highly abundant in the microbial world, CPS represents an emerging form of posttranslational regulation that is potentially widespread in microbes. Reactive chlorine species (RCS) are highly potent oxidants encountered by bacteria in a variety of natural environments, including within cells of the mammalian innate immune system. Here, we demonstrate that two naturally occurring RCS, namely, hypochlorous acid (the active compound in bleach) and N-chlorotaurine, can reversibly block splicing of DnaB inteins from Mycobacterium leprae and Mycobacterium smegmatis in vitro. Further, using a reporter that monitors DnaB intein activity within M. smegmatis, we show that DnaB protein splicing is inhibited by RCS in the native host. DnaB, an essential replicative helicase, is the most common intein-housing protein in bacteria. These results add to the growing list of environmental conditions that are relevant to the survival of the intein-containing host and influence protein splicing, as well as suggesting a novel mycobacterial response to RCS. We propose a model in which DnaB splicing, and therefore replication, is paused when these mycobacteria encounter RCS

A Period and a Prediction for the Of?p Spectrum Alternator HD 191612

The observational picture of the enigmatic O-type spectrum variable HD191612 has been sharpened substantially. A symmetrical, low-amplitude light curve with a period near 540 d has recently been reported from Hipparcos photometry. This period satisfies all of the spectroscopy since at least 1982, including extensive new observations during 2003 and 2004, and it has predicted the next transition during September--October 2004. Measurements of the H alpha equivalent width reveal a sharp emission peak in the phase diagram, in contrast to the apparently sinusoidal light curve. The He II absorption-line strength is essentially constant, while He I varies strongly, possibly filled in by emission in the O6 state, thus producing the apparent spectral-type variations. The O8 state appears to be the "normal" one. Two intermediate O7 observations have been obtained, which fall at the expected phases, but these are the only modern observations of the transitions so far. The period is too long for rotation or pulsation; although there is no direct evidence as yet for a companion, a model in which tidally induced oscillations drive an enhanced wind near periastron of an eccentric orbit appears promising. Further observations during the now predictable transitions may provide a critical test. Ultraviolet and X-ray observations during both states will likely also prove illuminating.Comment: 7 pages, 3 figures, 1 table; scheduled for the 2004 December 10 issue of ApJL, Vol. 617, No. 1. ApJ

Spectroscopic Sensitivity

We describe the overall performance of the STIS CCD after HST Servicing Mission #4 and the associated updates to calibration reference files. Most aspects of CCD performance are found to be fairly consistent with extrapolations of the trends seen prior to the failure of STIS in August 2004. The CCD gain value for the CCDGAIN = 4 setting has been redetermined using net count ratios of standard star spectra taken in the CCDGAIN = 1 and CCDGAIN = 4 settings, resulting in a gain value of 4.016 ± 0.003 e − /DN, which is 0.5% lower than the value used for the calibration of archival STIS CCD data taken before August 2004. Finally, we identify two independent indications of a temperature dependence of the Charge Transfer Efficiency (CTE). However, more calibration data are needed to verify the significance of this effect and, if verified, to calibrate it as a function of CCD housing temperature (as a proxy for CCD chip temperature). This option will be reassessed later during the Cycle 17 calibration program.

A CNO dichotomy among O2 giant spectra in the Magellanic Clouds

From a survey of the 3400 Å region in the earliest O-type spectra, we have found that two of the four O2 giants observed in the Large Magellanic Cloud have O IV lines there that are stronger than the N IV lines, while the other two have the opposite. A Small Magellanic Cloud counterpart also has N IV stronger than O IV. Inspection of the blue spectra of these stars shows that the former pair have weaker N lines in all ionization states (III, IV, and V) present as well as lines of C IV λ4658, while the latter three have stronger N lines and greater He/H. Space ultraviolet observations of two of the N-strong stars show N V wind profiles substantially stronger than those of C IV, while in the N-weak stars the C IV features are equal to or stronger than the N V. The N-strong stars are now reclassified as ON2 III(f *), newly defining that category. These characteristics strongly suggest a larger fraction of processed material in the atmospheres of the ON2 stars, which we confirm by modeling the optical spectra. In the context of current models, it is in turn implied that the ON2 stars are in a more advanced evolutionary state than the others, and/or that they had higher initial rotational velocities. The recent formulation of the effects of rotation on massive stellar evolution introduces an additional fundamental parameter, which the CNO abundances are in principle able to constrain. We present some illustrative comparisons with current Geneva evolutionary models for rotating massive stars. It is possible that these very hot, nitrogen-rich objects are products of homogeneous evolution. Our results will provide motivation for further physical modeling of the atmospheres and evolutionary histories of the most massive hot stars.Facultad de Ciencias Astronómicas y Geofísica

STIS UV spectroscopy of early B supergiants in M31

We analyze STIS spectra in the 1150-1700 Angstrom wavelength range obtained for six early B supergiants in the neighboring galaxy M31. Because of their likely high (nearly solar) abundance, these stars were originally chosen to be directly comparable to their Galactic counterparts, and represent a much-needed addition to our current sample of B-type supergiants, in our efforts to study the dependence of the Wind Momentum-Luminosity Relationship on spectral type and metallicity. As a first step to determine wind momenta we fit the P-Cygni profiles of the resonance lines of N V, Si IV and C IV with standard methods, and derive terminal velocities for all of the STIS targets. From these lines we also derive ionic stellar wind column densities. Our results are compared with those obtained previously in Galactic supergiants, and confirm earlier claims of `normal' wind line intensities and terminal velocities in M31. For half of the sample we find evidence for an enhanced maximum turbulent velocity when compared to Galactic counterparts.Comment: 15 pages, 9 figures, 2 tables. Accepted for publication in The Astrophysical Journa

Spectroscopic characterization of the known O-star population in Cygnus OB2. Evidence of multiple star-forming bursts

Context. Cygnus OB2 provides a unique insight into the high-mass stellar content in one of the largest groups of young massive stars in our Galaxy. Although several studies of its massive population have been carried out over the last decades, an extensive spectroscopic study of the whole known O-star population in the association is still lacking. Aims. We aim to carry out a spectroscopic characterization of all the currently known O stars in Cygnus OB2, determining the distribution of rotational velocities and accurate stellar parameters to obtain an improved view of the evolutionary status of the region. Methods. Based on existing and new optical spectroscopy, we performed a detailed quantitative spectroscopic analysis of all the known O-type stars identified in the association. For this purpose, we used the user-friendly iacob-broad and iacob-gbat automatized tools, FASTWIND stellar models, and astrometry provided by the Gaia second data release. Results. We created the most complete spectroscopic census of O stars carried out so far in Cygnus OB2 using already existing and new spectroscopy. We present the spectra for 78 O-type stars, from which we identify new binary systems, obtain the distribution of rotational velocities, and determine the main stellar parameters for all the stars in the region that have not been detected as double-line spectroscopic binaries. We also derive radii, luminosities, and masses for those stars with reliable Gaia astrometry, in addition to creating the Hertzsprung-Russell Diagram to interpret the evolutionary status of the association. Finally, we inspect the dynamical state of the population and identify runaway candidates. Conclusions. Our spectroscopic analysis of the O-star population in Cygnus OB2 has led to the discovery of two new binary systems and the determination of the main stellar parameters, including rotational velocities, luminosities, masses, and radii for all identified stars. This work has shown the improvement reached when using accurate spectroscopic parameters and astrometry for the interpretation of the evolutionary status of a population, revealing, in the case of Cygnus OB2, at least two star-forming bursts at ~3 and ~5 Myr. We find an apparent deficit of very fast rotators in the distribution of rotational velocities. The inspection of the dynamical distribution of the sample has allowed us to identify nine O stars with peculiar proper motions and discuss a possible dynamical ejection scenario or past supernova explosions in the region.We acknowledge financial support from the Spanish Ministry of Economy and Competitiveness (MINECO) under the grants AYA2012-39364-C02-01, AYA 2015-68012-C2-01, Severo Ochoa SEV-2015-0548 and PGC2018-093741-B-C21/C22 (MICIU/AEI/FEDER, UE)