Edge Detection on Eddy Current Image to Increase Defect Characterization

As the nuclear yard ages, the control of steam generator tubes (SGT) must deal with new problems. In fact new defects appear, especially in the area of the tube sheet, of the tube support or at the U-bend area. Eddy current testing using Rotating Probe Coil (absolute mode) gives a better resolution. These measurements allow smaller defects to be detected along different orientations, especially defects along the circumference in the rolling transition. Signals collected during the exploration of the tube internal wall with this coil contain the useful information which is represented in the form of a cartography (or image) for each of the signal complex components

Edge Detection on Eddy Current Image to Increase Defect Characterization

As the nuclear yard ages, the control of steam generator tubes (SGT) must deal with new problems. In fact new defects appear, especially in the area of the tube sheet, of the tube support or at the U-bend area. Eddy current testing using Rotating Probe Coil (absolute mode) gives a better resolution. These measurements allow smaller defects to be detected along different orientations, especially defects along the circumference in the rolling transition. Signals collected during the exploration of the tube internal wall with this coil contain the useful information which is represented in the form of a cartography (or image) for each of the signal complex components.</p

Leaf and root-associated fungal assemblages do not follow similar elevational diversity patterns

International audienceThe diversity of fungi along environmental gradients has been little explored in contrast to plants and animals. Consequently, environmental factors influencing the composition of fungal assemblages are poorly understood. The aim of this study was to determine whether the diversity and composition of leaf and root-associated fungal assemblages vary with elevation and to investigate potential explanatory variables. High-throughput sequencing of the Internal Transcribed Spacer 1 region was used to explore fungal assemblages along three elevation gradients, located in French mountainous regions. Beech forest was selected as a study system to minimise the host effect. The variation in species richness and specific composition was investigated for ascomycetes and basidiomycetes assemblages with a particular focus on root-associated ectomycorrhizal fungi. The richness of fungal communities associated with leaves or roots did not significantly relate to any of the tested environmental drivers, i.e. elevation, mean temperature, precipitation or edaphic variables such as soil pH or the ratio carbon: nitrogen. Nevertheless, the ascomycete species richness peaked at mid-temperature, illustrating a mid-domain effect model. We found that leaf and root-associated fungal assemblages did not follow similar patterns of composition with elevation. While the composition of the leaf-associated fungal assemblage correlated primarily with the mean annual temperature, the composition of root-associated fungal assemblage was explained equally by soil pH and by temperature. The ectomycorrhizal composition was also related to these variables. Our results therefore suggest that above and below-ground fungal assemblages are not controlled by the same main environmental variables. This may be due to the larger amplitude of climatic variables in the tree foliage compared to the soil environment

Sleep Stage Scoring Using the Neural Network Model: Comparison Between Visual and Automatic Analysis in Normal Subjects and Patients

International audienceIn this paper, we compare and analyze the results from automatic analysis and visual scoring of nocturnal sleep recordings. The validation is based on a sleep recording set of 60 subjects (33 males and 27 females), consisting of three groups: 20 normal control subjects, 20 depressed patients and 20 insomniac patients treated with a benzodiazepine. The inter-expert variability estimated from these 60 recordings (61,949 epochs) indicated an average agreement rate of 87.5% between two experts on the basis of 30-second epochs. The automatic scoring system, compared in the same way with one expert, achieved an average agreement rate of 82.3%, without expert supervision. By adding expert supervision for ambiguous and unknown epochs, detected by computation of an uncertainty index and unknown rejection, the automatic/expert agreement grew from 82.3% to 90%, with supervision over only 20% of the night. Bearing in mind the composition and the size of the test sample, the automated sleep staging system achieved a satisfactory performance level and may be considered a useful alternative to visual sleep stage scoring for large-scale investigations of human sleep

Finding candidate genes under positive selection in Non-model species: examples of genes involved in host specialization in pathogens

Chantier qualité GAInternational audienceNumerous genes in diverse organisms have been shown to be under positive selection, especially genes involved in reproduction, adaptation to contrasting environments, hybrid inviability, and host-pathogen interactions. Looking for genes under positive selection in pathogens has been a priority in efforts to investigate coevolution dynamics and to develop vaccines or drugs. To elucidate the functions involved in host specialization, here we aimed at identifying candidate sequences that could have evolved under positive selection among closely related pathogens specialized on different hosts. For this goal, we sequenced c. 17 000–32 000 ESTs from each of four Microbotryum species, which are fungal pathogens responsible for anther smut disease on host plants in the Caryophyllaceae. Forty-two of the 372 predicted orthologous genes showed significant signal of positive selection, which represents a good number of candidate genes for further investigation. Sequencing 16 of these genes in 9 additional Microbotryum species confirmed that they have indeed been rapidly evolving in the pathogen species specialized on different hosts. The genes showing significant signals of positive selection were putatively involved in nutrient uptake from the host, secondary metabolite synthesis and secretion, respiration under stressful conditions and stress response, hyphal growth and differentiation, and regulation of expression by other genes. Many of these genes had transmembrane domains and may therefore also be involved in pathogen recognition by the host. Our approach thus revealed fruitful and should be feasible for many non-model organisms for which candidate genes for diversifying selection are needed

Contribution of metagenomics to the study of mineral weathering bacterial communities in forest soils

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