The Local Edge Machine: inference of dynamic models of gene regulation

We present a novel approach, the Local Edge Machine, for the inference of regulatory interactions directly from time-series gene expression data. We demonstrate its performance, robustness, and scalability on in silico datasets with varying behaviors, sizes, and degrees of complexity. Moreover, we demonstrate its ability to incorporate biological prior information and make informative predictions on a well-characterized in vivo system using data from budding yeast that have been synchronized in the cell cycle. Finally, we use an atlas of transcription data in a mammalian circadian system to illustrate how the method can be used for discovery in the context of large complex networks.Department of Applied Mathematic

Phonon Quasidiffusion in Cryogenic Dark Matter Search Large Germanium Detectors

We present results on quasidi usion studies in large, 3 inch diameter, 1 inch thick [100] high purity germanium crystals, cooled to 50 mK in the vacuum of a dilution refrigerator, and exposed with 59.5 keV gamma-rays from an Am-241 calibration source. We compare data obtained in two di erent detector types, with di erent phonon sensor area coverage, with results from a Monte Carlo. The Monte Carlo includes phonon quasidi usion and the generation of phonons created by charge carriers as they are drifted across the detector by ionization readout channels.United States. Dept. of Energy (Grant DE-FG02-04ER41295)United States. Dept. of Energy (Grant DE-FG02-07ER41480)National Science Foundation (U.S.) (Grant PHY-0542066)National Science Foundation (U.S.) (Grant PHY-0503729)National Science Foundation (U.S.) (Grant PHY-0503629)National Science Foundation (U.S.) (Grant PHY-0504224)National Science Foundation (U.S.) (Grant PHY-0705078)National Science Foundation (U.S.) (Grant PHY-0801712

α-Synuclein interacts with lipoproteins in plasma

Parkinson’s disease (PD) is an age-related neurodegenerative disorder characterized by dopaminergic neural cell death in the substantia nigra of the brain and α-synuclein (α-syn) accumulation in Lewy bodies. α-Syn can be detected in blood and is a potential biomarker for PD. It has been shown recently that α-syn can pass through the blood-brain barrier (BBB), but the mechanism is not yet understood. We hypothesized that α-syn could interact with lipoproteins, and in association with these particles, could pass through the BBB. Here, we show that apoE, apoJ, and apoA1, but not apoB, were co-immunocaptured along with α-syn from human blood plasma, suggesting that α-syn is associated with high density lipoproteins (HDL). This association was also supported by experiments involving western blotting of plasma fractions separated by gel filtration,which revealed that α-syn was found in fractions identified as HDL. Interestingly, we could also detect α-syn and ApoJ in the intermediate fraction between HDL and LDL, referred to as lipoprotein (a) (Lp(a)), which has an important role in cholesterol metabolism. Overall, the results provide best support for the hypothesis that α-syn interacts with HDL, and this has potential implications for transport of α-syn from the brain to peripheral blood, across the BBB

Detector Fabrication Yield for SuperCDMS Soudan

The SuperCDMS collaboration is presently operating a 9 kg Ge payload at the Soudan Underground Laboratory in their direct search for dark matter. The Ge detectors utilize double-sided athermal phonon sensors with an interdigitated electrode structure (iZIPs) to reject near-surface electron-recoil events. These detectors each have a mass of 0.6 kg and were fabricated with photolithographic techniques. The detector fabrication advances required and the production yield encountered are described.United States. Dept. of EnergyNational Science Foundation (U.S.

My Adopted Child, There\u27s No One like You

https://stars.library.ucf.edu/diversefamilies/1514/thumbnail.jp

Have a new kid by Friday

Jakartaxxix, 396 p.; 21 c

Have a new Kid by Friday : Panduan cara mengubah attitude(sikap),Behavioour(Perilaku),Character(Karakter) anak anda dalam 5 hari

xxix, 396 p. : il; 21 cm