78 research outputs found
From edible to useful mushrooms - an attempt for the new economical assesment of large fungi
According to the stand of the modern applied mycological research the most commonly used term âedible mushroomâ does not express all significant aspects large fungi can be used for. Additionally to bioconversion for food and animal feed production there are at least three other fields where large fungi may also get economical relevance: for establishing of ectomycorrhiza, for medical application and for soil decontamination including environmental engineering. This new situation justifies the introduction of a new, all-embracing designation for large fungi. The term âuseful mushroomâ will be suggested. The various options of the use of mushrooms will be introduced and briefly discussed in this paper
Phenotypic, cytogenetic and spike fertility characterization of a population of male-sterile triticale
Sequence Composition and Gene Content of the Short Arm of Rye (Secale cereale) Chromosome 1
BACKGROUND: The purpose of the study is to elucidate the sequence composition of the short arm of rye chromosome 1 (Secale cereale) with special focus on its gene content, because this portion of the rye genome is an integrated part of several hundreds of bread wheat varieties worldwide. METHODOLOGY/PRINCIPAL FINDINGS: Multiple Displacement Amplification of 1RS DNA, obtained from flow sorted 1RS chromosomes, using 1RS ditelosomic wheat-rye addition line, and subsequent Roche 454FLX sequencing of this DNA yielded 195,313,589 bp sequence information. This quantity of sequence information resulted in 0.43Ă sequence coverage of the 1RS chromosome arm, permitting the identification of genes with estimated probability of 95%. A detailed analysis revealed that more than 5% of the 1RS sequence consisted of gene space, identifying at least 3,121 gene loci representing 1,882 different gene functions. Repetitive elements comprised about 72% of the 1RS sequence, Gypsy/Sabrina (13.3%) being the most abundant. More than four thousand simple sequence repeat (SSR) sites mostly located in gene related sequence reads were identified for possible marker development. The existence of chloroplast insertions in 1RS has been verified by identifying chimeric chloroplast-genomic sequence reads. Synteny analysis of 1RS to the full genomes of Oryza sativa and Brachypodium distachyon revealed that about half of the genes of 1RS correspond to the distal end of the short arm of rice chromosome 5 and the proximal region of the long arm of Brachypodium distachyon chromosome 2. Comparison of the gene content of 1RS to 1HS barley chromosome arm revealed high conservation of genes related to chromosome 5 of rice. CONCLUSIONS: The present study revealed the gene content and potential gene functions on this chromosome arm and demonstrated numerous sequence elements like SSRs and gene-related sequences, which can be utilised for future research as well as in breeding of wheat and rye
Genetic relationships and evolution in Cucurbita pepo (pumpkin, squash, gourd) as revealed by simple sequence repeat polymorphisms
Genetic relationships among 104 accessions of Cucurbita pepo were assessed from polymorphisms in 134 SSR (microsatellite) and four SCAR loci, yielding a total of 418 alleles, distributed among all 20 linkage groups. Genetic distance values were calculated, a dendrogram constructed, and principal coordinate analyses conducted. The results showed 100 of the accessions as distributed among three clusters representing each of the recognized subspecies, pepo, texana, and fraterna. The remaining four accessions, all having very small, round, striped fruits, assumed central positions between the two cultivated subspecies, pepo and texana, suggesting that they are relicts of undescribed wild ancestors of the two domesticated subspecies. In both, subsp. texana and subsp. pepo, accessions belonging to the same cultivar-group (fruit shape) associated with one another. Within subsp. pepo, accessions grown for their seeds or that are generalists, used for both seed and fruit consumption, assumed central positions. Specialized accessions, grown exclusively for consumption of their young fruits, or their mature fruit flesh, or seed oil extraction, tended to assume outlying positions, and the different specializations radiated outward from the center in different directions. Accessions of the longest-fruited cultivar-group, Cocozelle, radiated bidirectionally, indicating independent selection events for long fruits in subsp. pepo probably driven by a common desire to consume the young fruits. Among the accessions tested, there was no evidence for crossing between subspecies after domestication
The missing mushrooms: searching for fungi in ancient human dietary analysis
Fungi are a common part of modern human diets, but are rarely discussed in an archaeological context. Power et al. (2015) published data on bolete spores in human tooth calculus, suggesting that Upper Palaeolithic peoples ate mushrooms. Here we briefly consider the likelihood of mushroom consumption in the past, and examine whether or not stable isotopes may provide a way of seeing this in archaeological populations. We also consider the complexities of fungal stable isotopes using our own data and that from the literature. We conclude that fungi are highly variable isotopically, and are an additional dietary factor that should be considered when trying to interpret âterrestrialâ carbon isotope signatures combined with relatively high nitrogen isotope values in humans and other animals. Substantial mushroom ingestion could, in some cases, result in isotope values that may be interpreted as considerable meat consumption
Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L.
Until recently, only a few microsatellites have been available for Cucurbita, thus their development is highly desirable. The Austrian oil-pumpkin variety Gleisdorfer ĂlkĂźrbis (C. pepo subsp. pepo) and the C. moschata cultivar Soler (Puerto Rico) were used for SSR development. SSR-enriched partial genomic libraries were established and 2,400 clones were sequenced. Of these 1,058 (44%) contained an SSR at least four repeats long. Primers were designed for 532 SSRs; 500 primer pairs produced fragments of expected size. Of these, 405 (81%) amplified polymorphic fragments in a set of 12 genotypes: three C. moschata, one C. ecuadorensis, and eight C. pepo representing all eight cultivar groups. On an average, C. pepo and C. moschata produced 3.3 alleles per primer pair, showing high inter-species transferability. There were 187 SSR markers detecting polymorphism between the USA oil-pumpkin variety âLady Godivaâ (O5) and the Italian crookneck variety âBianco Friulanoâ (CN), which are the parents of our previous F2 mapping population. It has been used to construct the first published C. pepo map, containing mainly RAPD and AFLP markers. Now the updated map comprises 178 SSRs, 244 AFLPs, 230 RAPDs, five SCARs, and two morphological traits (h and B). It contains 20 linkage groups with a map density of 2.9Â cM. The observed genome coverage (Co) is 86.8%
Selenium level of the cultivated mushroom Agaricus bisporus
Selenium intake of the human population is very distinct and depends on the Se-content of consumed food. The higher intake of selenium can decrease the risk of many health problems in human and animal organisms. The main task of this work was to obtain new comparable data on Se content of Agaricus bisporus mushroom. The selenium content of different, common varieties of Agaricus bisporus and of its three cultivation's flushes was determined. The Se content of varieties varies between 0.46 mg kg-1 d.m. and 5.63 mg kg-1 d.m., and the average content is 2.82 (Âą 1.48) mg kg-1 d.m. The caps of fruit bodies have always higher selenium content than the stipe. The average cap/stipe selenium ratio is 1.29. The changes of Se concentration during the cultivation (in cultivation's flushes) are not significant. The most important cultivated mushroom species of the world (Agaricus bisporus) has, in addition to other more valuable properties, a remarkable Se-content. Consumption of fruit bodies can improve the Se supply of human organism, i.e. some health risks can be decreased
Chiasma distribution in rye chromosomes of diploid rye and in wheat/rye addition lines in relation to C-heterochromatin
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