An archaeal family-B DNA polymerase variant able to replicate past DNA damage: occurrence of replicative and translesion synthesis polymerases within the B family

A mutant of the high fidelity family-B DNA polymerase from the archaeon Thermococcus gorgonarius (Tgo-Pol), able to replicate past DNA lesions, is described. Gain of function requires replacement of the three amino acid loop region in the fingers domain of Tgo-Pol with a longer version, found naturally in eukaryotic Pol zeta (a family-B translesion synthesis polymerase). Inactivation of the 3'–5' proofreading exonuclease activity is also necessary. The resulting Tgo-Pol Z1 variant is proficient at initiating replication from base mismatches and can read through damaged bases, such as abasic sites and thymine photo-dimers. Tgo-Pol Z1 is also proficient at extending from primers that terminate opposite aberrant bases. The fidelity of Tgo-Pol Z1 is reduced, with amarked tendency tomake changes at G:C base pairs. Together, these results suggest that the loop region of the fingers domain may play a critical role in determining whether a family-B enzyme falls into the accurate genome-replicating category or is an errorprone translesion synthesis polymerase. Tgo-Pol Z1 may also be useful for amplification of damaged DNA

The beneficial and adverse effects of increasing N-3 polyunsaturated fatty acids (PUFA) intake on coronary heart disease (CHD) biomarkers

Available from British Library Document Supply Centre- DSC:DXN060671 / BLDSC - British Library Document Supply CentreSIGLEGBUnited Kingdo

Adaptive vibration condition monitoring technology for local tooth damage in gearboxes.

An adaptive approach was applied for local tooth damage diagnostics in gearboxes. The expediency of adaptation was proved experimentally for the new diagnostic feature, the sum of normalized sideband amplitudes. The positive correlation between mesh amplitudes and their sideband amplitudes was found experimentally for the first time. Novel adaptive vibration condition monitoring technology for local tooth damage in gearboxes was developed and experimentally validated. The experimental results showed an increase in effectiveness of the diagnostics when the adaptive technology was used

Päällystemerkinnät ja niiden rekisteröinti Oulun kaupungissa

Oulun kaupungissa on ollut kehittämistarpeita päällystemerkintöihin liittyen. Yksi opinnäytetyön tavoitteista oli luoda kaupungille päällystemerkintäperiaatteet, joista selviää, mitä merkintöjä tehdään millekin katuluokalle. Koska Oulussa tehdään suurin osa päällystemerkinnöistä maalamalla, toinen tavoite oli selvittää, minkälaisia vuosikustannuksia kestävämmät merkintämateriaalit aiheuttavat. Lisäksi kaupungilla oli tarve päällystemerkintärekisterille, josta saisi tietoa päällystemerkintöjen sijainneista ja lukumääristä. Kolmantena tavoitteena oli tehdä esiselvitys siitä, millä periaatteilla päällystemerkintärekisteriä kannattaa lähteä luomaan. Työssä tutkittiin eri päällystemerkintämateriaalien ominaisuuksia ja selvitettiin aikaisempien tutkimustulosten sekä kyselytutkimuksen avulla, miten eri materiaalit kestävät kulutusta eri liikennemäärillä. Tulosten perusteella laskettiin merkintämateriaaleista aiheutuvia vuosikustannuksia. Päällystemerkintäperiaatteita varten laadittiin katuluokitus sekä ohjeet siitä, miten päällystemerkinnät tehdään eri luokan kaduille. Lisäksi tutkittiin Tekla Civil Basicin soveltumista päällystemerkintärekisterin työkaluksi esimerkkitapausten perusteella. Päällystemerkintäperiaatteet tulevat helpottamaan jokakesäisiä kunnossapidon merkintätöitä Oulussa. Tutkimustulosten perusteella saatiin selville, että 1,5 mm:n vahvuinen spraymerkintä on vuosikustannuksiltaan kaikista edullisin. Päällystemerkintärekisterin luonti Tekla Civil Basicilla on teknisesti mahdollista.One purpose of this thesis was to develop principles for pavement markings in order to find out what kind of markings to use in different classes of streets. Most of the pavement markings in the city of Oulu are painted and there was a need for a research of the annual costs for other pavement marking materials. The city of Oulu had also a need for a register of pavement markings that could provide information about the locations and counts of the markings. Another purpose was to research the principles for creating the register. In this thesis the properties of different marking materials were examined. Additionally, it was found out how long the materials would last in different amounts of traffic. The research was made with the help of earlier investigations and a survey. The annual costs of the pavement markings were calculated from the results. Before composing the pavement marking principles, the streets had to be classified. In addition, it was studied, based on some example cases, how suitable tool Tekla Civil Basic would be for the pavement marking register. The pavement marking principles will facilitate the yearly maintenance work with pavement markings in the city of Oulu. Based on the results it was found out that markings made from 1.5 mm thick spray has the lowest annual costs. It was also found out that it is technically possible to create the pavement markings register with Tekla Civil Basic

Dataset for Increased dietary alpha-linolenic acid has sex-specific effects upon eicosapentaenoic acid status in humans: re-examination of data from a randomised, placebo-controlled, parallel study

Dataset supports: Childs, Caroline et al (2014) Increased dietary alpha-linolenic acid has sex-specific effects upon eicosapentaenoic acid status in humans: re-examination of data from a randomised, placebo-controlled, parallel study Nutrition Journal, 13, (113), pp. 1-5.</span

ApoE polymorphism and fish oil supplementation in subjects with an atherogenic lipoprotein phenotype

The study assessed the efficacy of fish oil supplementation in counteracting the classic dyslipidemia of the atherogenic lipoprotein phenotype (ALP). In addition, the impact of the common apolipoprotein E (apoE) polymorphism on the fasting and postprandial lipid profile and on responsiveness to the dietary intervention was established. Fifty-five ALP males (aged 34 to 69 years, body mass index 22 to 35 kg/m(2), triglyceride [TG] levels 1.5 to 4.0 mmol/L, high density lipoprotein cholesterol [HDL-C] 40% total LDL) completed a randomized placebo-controlled crossover trial of fish oil (3.0 g eicosapentaenoic acid/docosahexaenoic acid per day) and placebo (olive oil) capsules with the 6-week treatment arms separated by a 12-week washout period. In addition to fasting blood samples, at the end of each intervention arm, a postprandial assessment of lipid metabolism was carried out. Fish oil supplementation resulted in a reduction in fasting TG level of 35% (

Plant- and marine-derived n-3 polyunsaturated fatty acids have differential effects on fasting and postprandial blood lipid concentrations and on the susceptibility of LDL to oxidative modification in moderately hyperlipidemic subjects

Background: Dietary ?-linolenic acid (ALA) can be converted to long-chain n-3 polyunsaturated fatty acids (PUFAs) in humans and may reproduce some of the beneficial effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on cardiovascular disease risk factors.Objective: This study aimed to compare the effects of increased dietary intakes of ALA and EPA+DHA on a range of atherogenic risk factors.Design: This was a placebo-controlled, parallel study involving 150 moderately hyperlipidemic subjects randomly assigned to 1 of 5 interventions: 0.8 or 1.7 g EPA+DHA/d, 4.5 or 9.5 g ALA/d, or an n-6 PUFA control for 6 mo. Fatty acids were incorporated into 25 g of fat spread and 3 capsules to be consumed daily.Results: The change in fasting or postprandial lipid, glucose, or insulin concentrations or in blood pressure was not significantly different after any of the n-3 PUFA interventions compared with the n-6 PUFA control. The mean (± SEM) change in fasting triacylglycerols after the 1.7-g/d EPA+DHA intervention (-7.7 ± 4.99%) was significantly (P &lt; 0.05) different from the change after the 9.5-g/d ALA intervention (10.9 ± 4.5%). The ex vivo susceptibility of LDL to oxidation was higher after the 1.7-g/d EPA+DHA intervention than after the control and ALA interventions (P &lt; 0.05). There was no significant change in plasma ?-tocopherol concentrations or in whole plasma antioxidant status in any of the groups.Conclusion: At estimated biologically equivalent intakes, dietary ALA and EPA+DHA have different physiologic effects