44 research outputs found
Coupled structural and magnetic properties of ferric fluoride nanostructures part I: a Metropolis atomistic study
A modified Metropolis atomistic simulation is proposed to model the structure
of grain boundaries (GBs) and interfaces in ionic nanostructured systems and is
applied to the magnetically interesting case of iron trifluoride (FeF3). We
chose long-range interatomic potentials adjusted on experimental results, and
adapted a previously established Monte Carlo scheme consisting of various
modifications of the simulated annealing/ Metropolis algorithm. Atomic
structures of twisted and tilted GBs as a function of the relative
disorientation of the grains have been achieved yielding close to
experimentally measured properties. This approach takes into account the
structure of the grains far from the interface in order to constrain the
relative orientation of the grains, without any periodic boundary conditions.
One concludes that a long-range coulombic falloff of the interatomic potentials
is necessary to obtain GB structures presenting a correct local topology but
with a smooth transition from crystalline to amorphous states. The structural
features are finally discussed in terms of topological aspects and local
magnetic structure.Comment: submitted to JMM
Gla-rich protein function as an anti-inflammatory agent in monocytes/macrophages: implications for calcification-related chronic inflammatory diseases
Calcification-related chronic inflammatory diseases are multifactorial pathological processes, involving a complex interplay between inflammation and calcification events in a positive feed-back loop driving disease progression. Gla-rich protein (GRP) is a vitamin K dependent protein (VKDP) shown to function as a calcification inhibitor in cardiovascular and articular tissues, and proposed as an anti-inflammatory agent in chondrocytes and synoviocytes, acting as a new crosstalk factor between these two interconnected events in osteoarthritis. However, a possible function of GRP in the immune system has never been studied. Here we focused our investigation in the involvement of GRP in the cell inflammatory response mechanisms, using a combination of freshly isolated human leucocytes and undifferentiated/differentiated THP-1 cell line. Our results demonstrate that VKDPs such as GRP and matrix gla protein (MGP) are synthesized and gamma-carboxylated in the majority of human immune system cells either involved in innate or adaptive immune responses. Stimulation of THP-1 monocytes/macrophages with LPS or hydroxyapatite (HA) up-regulated GRP expression, and treatments with GRP or GRP-coated basic calcium phosphate crystals resulted in the down-regulation of mediators of inflammation and inflammatory cytokines, independently of the protein gamma-carboxylation status. Moreover, overexpression of GRP in THP-1 cells rescued the inflammation induced by LPS and HA, by down-regulation of the proinflammatory cytokines TNF alpha, IL-1 beta and NFkB. Interestingly, GRP was detected at protein and mRNA levels in extracellular vesicles released by macrophages, which may act as vehicles for extracellular trafficking and release. Our data indicate GRP as an endogenous mediator of inflammatory responses acting as an anti-inflammatory agent in monocytes/macrophages. We propose that in a context of chronic inflammation and calcification-related pathologies, GRP might act as a novel molecular mediator linking inflammation and calcification events, with potential therapeutic application.Portuguese Science and Technology Foundation (FCT) [PTDC/SAU-ORG/117266/2010, PTDC/BIM-MEC/1168/2012, UID/Multi/ 04326/2013]; FCT fellowships [SFRH/BPD/70277/2010, SFRH/BD/111824/2015
Imaging atherosclerosis in rheumatoid arthritis: evidence for increased prevalence, altered phenotype and a link between systemic and localised plaque inflammation.
In rheumatoid arthritis (RA), chronic inflammation is thought to drive increased cardiovascular risk through accelerated atherosclerosis. It may also lead to a more high-risk plaque phenotype. We sought to investigate carotid plaque phenotype in RA patients using Dynamic Contrast-Enhanced MRI (DCE-MRI) and Fludeoxyglucose Positron Emission Tomography(FDG-PET). In this pilot study, RA patients and age/sex-matched controls were evaluated for cardiovascular risk factors and carotid plaque on ultrasound. Subjects with plaque >2âmm thick underwent DCE-MRI, and a subgroup of patients had FDG-PET. Comparison of MRI findings between groups and correlation between clinical, serological markers and imaging findings was undertaken. 130 patients and 62 controls were recruited. Plaque was more prevalent in the RA group (53.1% vs 37.0%, pâ=â0.038) and was independently associated with IL6 levels (HR[95%CI]: 2.03 [1.26, 3.26] per quartile). DCE-MRI data were available in 15 patients and 5 controls. Higher prevalence of plaque calcification was noted in RA, despite similar plaque size (73.3% vs 20%, pâ=â0.04). FDG-PET detected plaque inflammation in 12/13 patients scanned and degree of inflammation correlated with hs-CRP (râ=â0.58, pâ=â0.04). This study confirms increased prevalence of atherosclerosis in RA and provides data to support the hypothesis that patients have a high-risk plaque phenotype
Nuclear magnetic resonance quadrupolar parameters and short range order in disordered ionic fluorides
International audienc
Investigation of fluorine octahedron connectivities in transition metal fluoride glasses by solid-state magic-angle-spinning nuclear magnetic resonance spectroscopy
International audienc
From crystalline to glassy gallium fluoride materials: an NMR study of and quadrupolar nuclei
International audienc
Double-quantum 19F-19F dipolar recoupling at ultra-fast magic angle spinning NMR: Apllication to the assignment of 19F spectra of inorganic fluorides
International audienc