Let them brew! Reflexivity, and division of labour in deliberation for science and technology governance

This thesis examines the theoretical premises of and ways that macro deliberative approaches to decision making function in application to specific instances of science and technology governance. Macro-level deliberations constitute complex, extended, distributed decision making processes, in contrast to individual micro deliberation exercises undertaken in particular settings. Macro deliberations employ the mechanism of ‘division of labour’ in terms of actors, tasks and methods in order to secure the two essential qualities of ‘inclusiveness’ and ‘deliberativeness’ – thus resolving the inherent tension between number of participants and deep discussion. Accordingly, the thesis focuses on the ways in which this paradoxical mechanism of ‘inclusion by division’ functions in macro deliberations. An interrogation of two UK nationwide public deliberation cases – GM Dialogue (on GM crops) and the CoRWM process (on radioactive waste) – sheds light on the significant role of reflexivity in such macro deliberative approaches to decision making. The thesis adopts a triangulated approach towards both documents and interviews employing contending representations to cross-check the one with the other. In considering the ways in which reflexivity constitutes a critical quality of the process and outcome of division of labour in macro deliberations, the thesis argues that the notion of reflexivity is central to explaining how macro deliberation functions: The reflective and self-contingent feature of reflexivity enables participants to explore diverse rationales on division of labour through continuous generation of new rationales; this recursive self-reconfiguration process of rationales on division of labour entails an evolutionary development of division of labour. As division of labour is played out not in a static, exogenous fashion, but through a dynamic, endogenous construction process, reflexivity in real-world macro deliberations illuminates some significant contrasts in the ways that ‘deliberation’ and ‘inclusion’ take place to those characterised in theory. Indeed, deliberation emerges in practice as more than just open rational dialogue. In order to understand this more fully, it must be seen in terms of diversity of material, social and political interactions, and relationships – referred to here as ‘discursive relations’. In reality, then, inclusion occurs in more emergent ways than intended by design, rather, unfolding as participants engage with each other. In this way, actors’ divergent views are cross-reflected and mutually influence each other, not through theoretically-envisaged top-down aggregation but via a kind of endogenous ‘fermentation’ process. In this way, reflexivity actually makes macro public deliberation a more effectively inclusive and deliberative decision making process. In short, recognition of this inherent reflexivity in macro deliberations offers practically to aid improved understanding of the complex process of engagement in science and technology governance. It suggests that we would benefit from shifting our attention somewhat away from the direct provision of strictly prescriptive design protocols towards the construction of better general environments for facilitating more reflexivity, which should enable actors to shape their own reflexive deliberation. Then let them brew

Phosphorylated claspin interacts with a phosphate-binding site in the kinase domain of Chk1 during ATR-mediated activation

Claspin is essential for the ATR-dependent activation of Chk1 in Xenopus egg extracts containing incompletely replicated or UV-damaged DNA. The activated form of Claspin contains two repeated phosphopeptide motifs that mediate its binding to Chk1. We show that these phosphopeptide motifs bind to Chk1 by means of its N-terminal kinase domain. The binding site on Chk1 involves a positively charged cluster of amino acids that contains lysine 54, arginine 129, threonine 153, and arginine 162. Mutagenesis of these residues strongly compromises the ability of Chk1 to interact with Claspin. These amino acids lie within regions of Chk1 that are involved in various aspects of its catalytic function. The predicted position on Chk1 of the phosphate group from Claspin corresponds to the location of activation-loop phosphorylation in various kinases. In addition, we have obtained evidence that the C-terminal regulatory domain of Chk1, which does not form a stable complex with Claspin under our assay conditions, nonetheless has some role in Claspin-dependent activation. Overall, these results indicate that Claspin docks with a phosphate-binding site in the catalytic domain of Chk1 during activation by ATR. Phosphorylated Claspin may mimic an activating phosphorylation of Chk1 during this process

The PCA Filtering method for an unbiased spectral survey of Complex Organic Molecules (COMs)

A variety of interstellar complex organic molecules (COMs) have been detected in various physical conditions. However, in the protostellar and protoplanetary environments, their complex kinematics make line profiles blend each other and the line strength of weak lines weaker. In this paper, we utilize the principal component analysis (PCA) technique to develop a filtering method which can extract COM spectra from the main kinematic component associated with COM emission and increase the signal-to-noise ratio (SNR) of spectra. This filtering method corrects non-Gaussian line profiles caused by the kinematics. For this development, we adopt the ALMA BAND 6 spectral survey data of V883 Ori, an eruptive young star with a Keplerian disk. A filter was, first, created using 34 strong and well-isolated COM lines and then applied to the entire spectral range of the dataset. The first principal component (PC1) describes the most common emission structure of the selected lines, which is confined within the water sublimation radius ($\sim$ 0.3 arcsec) in the Keplerian disk of V883 Ori. Using this PC1 filter, we extracted high-SNR kinematics-corrected spectra of V883 Ori over the entire spectral coverage of $\sim$50 GHz. The PC1-filtering method reduces the noise by a factor of $\sim$ 2 compared to the average spectra over the COM emission region. One important advantage of this PC1-filtering method over the previously developed matched filtering method is to preserve the original integrated intensities of COM lines.Comment: 24 pages, 13 figure

Topology of Luminous Red Galaxies from the Sloan Digital Sky Survey

We present measurements of the genus topology of luminous red galaxies (LRGs) from the Sloan Digital Sky Survey (SDSS) Data Release 7 catalog, with unprecedented statistical significance. To estimate the uncertainties in the measured genus, we construct 81 mock SDSS LRG surveys along the past light cone from the Horizon Run 3, one of the largest N-body simulations to date that evolved 7210^3 particles in a 10815 Mpc/h size box. After carefully modeling and removing all known systematic effects due to finite pixel size, survey boundary, radial and angular selection functions, shot noise and galaxy biasing, we find the observed genus amplitude to reach 272 at 22 Mpc/h smoothing scale with an uncertainty of 4.2%; the estimated error fully incorporates cosmic variance. This is the most accurate constraint of the genus amplitude to date, which significantly improves on our previous results. In particular, the shape of the genus curve agrees very well with the mean topology of the SDSS LRG mock surveys in the LCDM universe. However, comparison with simulations also shows small deviations of the observed genus curve from the theoretical expectation for Gaussian initial conditions. While these discrepancies are mainly driven by known systematic effects such as those of shot noise and redshift-space distortions, they do contain important cosmological information on the physical effects connected with galaxy formation, gravitational evolution and primordial non-Gaussianity. We address here the key role played by systematics on the genus curve, and show how to accurately correct for their effects to recover the topology of the underlying matter. In a forthcoming paper, we provide an interpretation of those deviations in the context of the local model of non-Gaussianity.Comment: 23 pages, 18 figures. APJ Supplement Series 201