277 research outputs found
Change in gene expression of mouse embryonic stem cells derived from parthenogenetic activation
BACKGROUND We previously established parthenogenetic mouse embryonic stem cells (ESCs) and this study was subsequently conducted for elucidating the influence of oocyte parthenogenesis on gene expression profile of ESCs. METHODS Gene expression of parthenogenetic ESC (pESC)-1 or pESC-2 was separately compared with that of two normally fertilized ESC (nfESC) lines (B6D2F1 and R1 strains), and quantification of mRNA expression was conducted for validating microarray data. RESULTS In two sets of comparison, reaction of 11 347 and 15 454 gene probes were altered by parthenogenesis, while strain difference changed the expression of 15 750 and 14 944 probes. Level of correlation coefficient was higher in the comparisons between normal fertilization and parthenogenesis (0.974-0.985) than in the comparisons between strains of nfESCs (0.97-0.971). Overall, the expression of 3276-3329 genes was changed after parthenogenesis, and 88% (96/109) of major functional genes differentially (P < 0.01) expressed in one comparison set showed the same change in the other. When we monitored imprinted genes, expression of nine paternal and eight maternal genes were altered after parthenogenesis and 88% (14/16) of these was confirmed by mRNA quantification. CONCLUSIONS The change in gene expression after parthenogenesis was similar to, or less than, the change induced by a strain difference under a certain genetic background. These results may suggest the clinical feasibility of parthenogenesis-derived, pluripotent cell
Surface Characteristics of Orthodontic Materials and Their Effects on Adhesion of Mutans streptococci
Objective: To test the hypothesis that there are no significant differences in the adhesion of mutans streptococci (MS) to various orthodontic materials based on their surface characteristics.
Materials and Methods: Surface roughness (SR) and surface free energy (SFE) characteristics were investigated for nine different orthodontic materials (four orthodontic adhesives, three bracket raw materials, hydroxyapatite blocks, and bovine incisors) using confocal laser scanning microscopy and sessile drop method. Each material, except the bovine incisors, was incubated with whole saliva or phosphate-buffered saline for 2 hours. Adhesion assays were performed by incubating tritium-labeled MS with each material for 3 or 6 hours.
Results: Orthodontic adhesives had higher SFE characteristics and lower SR than bracket materials. Orthodontic adhesives showed a higher MS retaining capacity than bracket materials, and MS adhesion to resin-modified glass ionomer and hydroxyapatite was highest. Extended incubation time increased MS adhesion, while saliva coating did not significantly influence MS adhesion. SFE, specifically its dispersive and polar components, was positively correlated with MS adhesion, irrespective of saliva coating.
Conclusions: The hypothesis is rejected. This study suggests that SFE characteristics play an important role in the initial MS adhesion to orthodontic materials.This study was supported by a grant from the Korea Health
21 Project, Ministry of Health and Welfare, Republic of Korea
(03-PJ1-PG1-CH09-0001)
High-resolution near-IR Spectral mapping with H and [Fe II] lines of Multiple Outflows around LkH 234
We present a high-resolution, near-IR spectroscopic study of multiple
outflows in the LkH 234 star formation region using the Immersion
GRating INfrared Spectrometer (IGRINS). Spectral mapping over the blueshifted
emission of HH 167 allowed us to distinguish at least three separate, spatially
overlapped, outflows in H and [Fe II] emission. We show that the H
emission represents not a single jet, but complex multiple outflows driven by
three known embedded sources: MM1, VLA 2, and VLA 3. There is a redshifted
H outflow at a low velocity, \VLSR 50 {\kms}, with respect to
the systemic velocity of \VLSR 11.5 {\kms}, that coincides with the
HO masers seen in earlier radio observations two arcseconds southwest of
VLA 2. We found that the previously detected [Fe II] jet with \VLSR
100 {\kms} driven by VLA 3B is also detected in H emission, and confirm
that this jet has a position angle about 240. Spectra of the
redshifted knots at 14\arcsec65\arcsec northeast of LkH 234 are
presented for the first time. These spectra also provide clues to the existence
of multiple outflows. We detected high-velocity (50120 {\kms}) H gas
in the multiple outflows around LkH 234. Since these gases move at
speeds well over the dissociation velocity ( 40 {\kms}), the emission must
originate from the jet itself rather than H gas in the ambient medium.
Also, position-velocity diagrams and excitation diagram indicate that emission
from knot C in HH 167 come from two different phenomena, shocks and
photodissociation.Comment: 32 pages, 12 figures, 2 tables, Accepted for publication in the
Astrophysical Journa
UKIRT Widefield Infrared Survey for Fe+
The United Kingdom Infrared Telescope (UKIRT)Widefield Infrared Survey for Fe+ (UWIFE) is a 180 deg2 imaging survey of the first Galactic quadrant (7° < l < 62° |b| <1°.5) that uses a narrow-band filter centred on the [Fe II] 1.644-μm emission line. The [Fe II] 1.644-μm emission is a good tracer of dense, shock-excited gas, and the survey will probe violent environments around stars: star-forming regions, evolved stars, and supernova remnants, among others. The UWIFE survey is designed to complement the existing UKIRTW idefield Infrared Survey for H2 (UWISH2). The survey will also complement existing broad-band surveys. The observed images have a nominal 5Ï? detection limit of 18.7 mag for point sources, with a median seeing of 0.83 arcsec. For extended sources, we estimate a surface brightness limit of 8.1 Ã? 10-20 W m-2 arcsec-2. In this paper, we present an overview and some preliminary results of this survey. © 2014 The Authors Published by Oxford University Press on behalf of the Royal Astronomical Society
Dense Iron Ejecta and Core-collapse Supernova Explosion in the Young Supernova Remnant G11.2-0.3
We present the results of near-infrared spectroscopic observations of dense
(\simgt 10 cm) iron ejecta in the young core-collapse supernova
remnant G11.2-0.3. Five ejecta knots projected to be close to its center show a
large dispersion in their Doppler shifts: two knots in the east are blueshifted
by more than 1,000 \kms, while three western knots have relatively small
blueshifts of 20-60 \kms. This velocity discrepancy may indicate that the
western knots have been significantly decelerated or that there exists a
systematic velocity difference among the knots. One ejecta filament in the
northwestern boundary, on the other hand, is redshifted by \simgt 200 \kms,
while opposite filament in the southeastern boundary shows a negligible radial
motion. Some of the knots and filaments have secondary velocity components, and
one knot shows a bow shock-like feature in the velocity structure. The iron
ejecta appear to be devoid of strong emission from other heavy elements, such
as S, which may attest to the alpha-rich freezeout process in the explosive
nucleosynthesis of the core-collapse supernova explosion close to its center.
The prominent bipolar distribution of the Fe ejecta in the northwestern and
southeastern direction, along with the elongation of the central pulsar wind
nebula in the perpendicular direction, is consistent with the interpretation
that the supernova exploded primarily along the northwestern and southeastern
direction.Comment: To appear in ApJ Letter
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