In vitro viability of genipap pollen grains in different culture media

O objetivo deste trabalho foi avaliar a viabilidade in vitro do grão de pólen de jenipapeiro (Genipa americana) à temperatura ambiente, em diferentes meios de cultura. O delineamento experimental foi inteiramente casualizado em arranjo fatorial 3x11 (meios de cultura x tempos de incubação), com quatro repetições. O número de grãos de pólen germinados foi analisado em intervalos de 24 horas até 288 horas após incubação em temperatura ambiente. O meio com 100 mg ha-1 de H3BO3, 80 g L-1 de sacarose e 1,0 L-1 de ágar resulta em alta à média percentagem de germinação in vitro, sendo o mais adequado para estudos da viabilidade in vitro de grãos de pólen de jenipapeiro.The objective of this work was to evaluate the viability of genipap (Genipa americana) pollen grain at room temperature in different culture media. The experimental design was completely randomized in a 3x11 factorial arrangement (culture media x incubation times) with four replicates. The number of germinated pollen grains was analyzed at 24-hour intervals up to 288 hours after incubation at room temperature. The culture medium with 100 mg L-1 H3BO3, 80 g L-1 sucrose, and 1.0 g L-1 agar results in a higher to intermediate germination percentage, being the most suitable for studies on the in vitro viability of genipap pollen grains

AVALIAÇÃO INICIAL DE PROGÊNIES DE MANGABEIRA

A mangabeira (Hancornia speciosa Gomes) é uma espécie frutífera nativa do Brasil. A avaliação de características de crescimento se torna importante em pesquisas de melhoramento genético e podem estar correlacionadas positivamente com produtividade. O presente trabalho objetivou avaliar aspectos iniciais de desenvolvimento em um teste de progênie, que possibilite fundamentos para conservação da mangabeira. Foram avaliadas as variáveis: altura das mudas, diâmetro do coleto e sobrevivência. O delineamento experimental utilizado foi em blocos casualizados, em que os tratamentos consistiram nos diferentes acessos utilizados na produção das mudas e nos tempos de avaliação. Para comparação do tempo de crescimento foi utilizado o teste de Tukey (

Cambuí (Myrciaria floribunda (West ex Willd.) O. Berg): A potential nutritional supplement for inhibition of COVID-19 infections / Cambuí (Myrciaria floribunda (West ex Willd.) O. Berg): Um potencial suplemento nutricional para a inibição de infecções por COVID-19

In the present study, we suggest that the Myrciaria floribunda (West ex Willd.) O. Berg fruit (cambuí) is a natural source of potential molecules against COVID-19. Extracts from orange and purple cambuí were obtained by using pressurized hot water extraction (PHWE). Extraction overall yield was 39.66 ± 1.62% and 33.00 ± 8.44% for the extracts of purple and orange cambuí, respectively. All analyzed extracts showed high total phenolic contents, especially those of purple fruits (3870.37 ± 353.09 mg GAE/100g). The total flavonoid content was 693.16 ± 180.09 and 770.08 ± 103.62 mg RE/100g for purple and orange fruit extracts, respectively. The secondary metabolite profile exhibited ellagic acid, ellagitannins, and derivatives of caffeic acid and ellagic acid. PHWE showed a good alternative for molecules separation from cambuí with promising potential for further in vitro and in vivo evaluation against Covid-19 infections

Callogénesis y caracterización morfohistológica de Hancornia speciosa Gomes

Hancornia speciosa Gomes belongs to the family Apocynaceae and is distributed across different regions of Brazil. The objective of this study was to evaluate callus induction and histodifferentiation in five mangaba accessions under different in vitro culture conditions. Five acessions from the Active Germplasm Bank of Mangaba (Embrapa, Brazil) were evaluated. In vitro-germinated plant seedlings were used for the excision of different explants (internode and node segments, and foliar section). These segments were inoculated in a culture medium containing different concentrations of 6-Benzylaminopurine (BA) and 2,4-Dichlorophenoxyacetic acid (2,4-D). There was a significant effect of accessions, treatments, and time on the callus mass (g). There was no callus induction in the absence of regulators. After 60 days of in vitro culture, all treatments exhibited a linear positive cellular growth. The highest callus mass was observed in the BI accession, in the presence of 22.62 µM 2,4-D and 11.10 µM BA.Hancornia speciosa Gomes pertenece a la familia Apocynaceae, distribuida en diferentes regiones de Brasil. El objetivo de este trabajo fue evaluar la callogénesis y la histodiferenciación en cinco accesiones de mangaba bajo diferentes condiciones de cultivo in vitro. Se evaluaron cinco accesiones del Banco de Germoplasma Activo de Mangaba (Embrapa, Brasil). Las plántulas germinadas in vitro se utilizaron para la escisión de explantes (segmentos nodales e internodales y secciones foliares). Estos segmentos fueron inoculados en un medio de cultivo que contenía diferentes concentraciones de 6-benzilaminopurina (BA) y ácido 2,4-diclorofenoxiacético (2,4-D). Hubo un efecto significativo de las accesiones, los tratamientos y el tiempo del cultivo en la masa de los callos. No hubo inducción de callos en ausencia de reguladores. A los 60 días de cultivo in vitro, los otros tratamientos presentaron un crecimiento celular linear positivo. La mayor masa de callo se observó en la accesión BI, en presencia de 22,62 μM 2,4-D y 11,10 μM BA

Identification and preliminary characterization of early fruiting mangabeira (Hancornia speciosa – Apocynaceae)

The mangabeira (Hancornia speciosa G.) is a native Brazilian species in the process of domestication. It has several potential uses. In recent years the populations of mangabeira have become fragmented and, to conserve their genetic resources, Embrapa Tabuleiros Costeiros has, since 2006, maintained a Mangaba Genebank, which currently has 299 individuals distributed across 27 accessions. In one of these accessions (CP), first fruiting was observed to occur at 13 months, indicating precocity. The current project was developed with the intention of morphologically characterizing the CP accessions and evaluating the extent of genetic diversity between genotypes, based on genetic markers associated with fruiting precocity. Morphological variation in crown and leaf shape, growth habit, total height, stem diameter and canopy height were investigated. For molecular characterization, 18 CP accession genotypes were used, all from Sergipe State, Brazil, and 11 ISSR markers were studied that generated 62 fragments with 91.4% polymorphism. The fruits have physical and chemical characteristics suitable for in natura consumption and also for processing. UPGMA dendrogram analysis showed the genotypes CP2 and CP6 to be most similar and CP10 the most divergent within the CP accessions. The results showed the existence of polymorphism, and genetic divergence among genotypes, thus reinforcing the importance of constant germplasm characterization, which guarantees information on gene sources for future use, while providing information concerning potential parents that can be used in breeding programs as well as the identification of duplicates

Conservação in vitro de germoplasma de abacaxi tratado com paclobutrazol

The aim of this work was to evaluate the effect of paclobutrazol (PBZ) on in vitro growth and conservation of pineapple germplasm. Plants of the PE x SC-60 pineapple hybrid were cultivated on MS medium supplemented with 30 g L-1 of sucrose and 8 g L-1 of agar. Treatments consisted of two concentrations of PBZ, the first one applied at the beginning of the trial and the other ninety days after. The best result was obtained without PBZ or with 0.5 mg L-1 at the beginning of the trial. It was possible to reduce the number of subcultures during conservation.O objetivo deste trabalho foi avaliar o efeito do paclobutrazol (PBZ) no crescimento in vitro de plantas de abacaxi visando à conservação do germoplasma. Utilizou-se o meio MS suplementado com 30 g L-1 de sacarose e 8 g L-1 de ágar. Cada tratamento consistiu de duas doses de PBZ: a primeira aplicada no início do experimento e a segunda, noventa dias após, em combinações que envolviam a ausência, 0,5 e 1,0 mg L-1. Os melhores resultados foram obtidos na ausência do PBZ, ou com 0,5 mg L-1 aplicada apenas no início do experimento. Foi possível reduzir o número de subcultivos durante o período de conservação

Conservação in vitro de germoplasma de abacaxi tratado com paclobutrazol

The aim of this work was to evaluate the effect of paclobutrazol (PBZ) on in vitro growth and conservation of pineapple germplasm. Plants of the PE x SC-60 pineapple hybrid were cultivated on MS medium supplemented with 30 g L-1 of sucrose and 8 g L-1 of agar. Treatments consisted of two concentrations of PBZ, the first one applied at the beginning of the trial and the other ninety days after. The best result was obtained without PBZ or with 0.5 mg L-1 at the beginning of the trial. It was possible to reduce the number of subcultures during conservation.O objetivo deste trabalho foi avaliar o efeito do paclobutrazol (PBZ) no crescimento in vitro de plantas de abacaxi visando à conservação do germoplasma. Utilizou-se o meio MS suplementado com 30 g L-1 de sacarose e 8 g L-1 de ágar. Cada tratamento consistiu de duas doses de PBZ: a primeira aplicada no início do experimento e a segunda, noventa dias após, em combinações que envolviam a ausência, 0,5 e 1,0 mg L-1. Os melhores resultados foram obtidos na ausência do PBZ, ou com 0,5 mg L-1 aplicada apenas no início do experimento. Foi possível reduzir o número de subcultivos durante o período de conservação