Magnetostratigraphy of the Hell Creek and lower Fort Union formations in northeast Montana

Magnetostratigraphic evaluation of a well-exposed stratigraphic section in northeast Montana has been undertaken to expand upon and better understand the timing of the deposition of Hell Creek and Fort Union Formations. Characteristic remnant magnetizations show clear magnetostratigraphic patterning of chrons C28n, C28r, C29n, C29r, C30n, and possibly C30r. Differentially corrected GPS coordinates, including elevation, were recorded at each sample site allowing the magnetostratigraphic framework to be precisely relocated in the field and traced laterally across the landscape. Localities in Montana that have been sampled for fossil studies have been mapped and correlated to the same stratigraphic sections as the magnetostratigraphy, and so can be compared directly to the geomagnetic polarity time scale. The new magnetostratigraphy can also be used to relate to other basins of Cretaceous and Paleogene age using information independent from biostratigraphic zonation, making it possible to directly compare the composition of coeval faunas from significantly different latitudes

The role of microhomology in genomic structural variation

Genomic structural variation, which can be defined as differences in the copy number, orientation, or location of relatively large DNA segments, is not only crucial in evolution, but also gives rise to genomic disorders. Whereas the major mechanisms that generate structural variation have been well characterised, insights into additional mechanisms are emerging from the identification of short regions of DNA sequence homology, also known as microhomology, at chromosomal breakpoints. In addition, functional studies are elucidating the characteristics of microhomology-mediated pathways, which are mutagenic. Here, we describe the features and mechanistic models of microhomology-mediated events, discuss their physiological and pathological significance, and highlight recent advances in this rapidly evolving field of research

Climate change reduces the net sink of CH\u3csub\u3e4\u3c/sub\u3e and N\u3csub\u3e2\u3c/sub\u3eO in a semiarid grassland

Atmospheric concentrations of methane (CH4) and nitrous oxide (N2O) have increased over the last 150 years because of human activity. Soils are important sources and sinks of both potent greenhouse gases where their production and consumption are largely regulated by biological processes. Climate change could alter these processes thereby affecting both rate and direction of their exchange with the atmosphere. We examined how a rise in atmospheric CO2 and temperature affected CH4 and N2O fluxes in a well-drained upland soil (volumetric water content ranging between 6% and 23%) in a semiarid grassland during five growing seasons. We hypothesized that responses of CH4 and N2O fluxes to elevated CO2 and warming would be driven primarily by treatment effects on soil moisture. Previously we showed that elevated CO2 increased and warming decreased soil moisture in this grassland. We therefore expected that elevated CO2 and warming would have opposing effects on CH4 and N2O fluxes. Methane was taken up throughout the growing season in all 5 years. A bell-shaped relationship was observed with soil moisture with highest CH4 uptake at intermediate soil moisture. Both N2O emission and uptake occurred at our site with some years showing cumulative N2O emission and other years showing cumulative N2O uptake. Nitrous oxide exchange switched from net uptake to net emission with increasing soil moisture. In contrast to our hypothesis, both elevated CO2 and warming reduced the sink of CH4 and N2O expressed in CO2 equivalents (across 5 years by 7% and 11% for elevated CO2 and warming respectively) suggesting that soil moisture changes were not solely responsible for this reduction. We conclude that in a future climate this semiarid grassland may become a smaller sink for atmospheric CH4 and N2O expressed in CO2-equivalents

Germination thresholds of species in the mixed-grass prairie as affected by global climate change: a FACE study

Non-Peer Reviewe

Microclimatic Performance of a Free-Air Warming and CO\u3csub\u3e2\u3c/sub\u3e Enrichment Experiment in Windy Wyoming, USA

In order to plan for global changing climate experiments are being conducted in many countries, but few have monitored the effects of the climate change treatments (warming, elevated CO2) on the experimental plot microclimate. During three years of an eight year study with year-round feedback-controlled infra-red heater warming (1.5/3.0°C day/night) and growing season free-air CO2 enrichment (600 ppm) in the mixed-grass prairie of Wyoming, USA, we monitored soil, leaf, canopy-air, above-canopy-air temperatures and relative humidity of control and treated experimental plots and evaluated ecologically important temperature differentials. Leaves were warmed somewhat less than the target settings (1.1 & 1.5°C day/night) but soil was warmed more creating an average that matched the target settings extremely well both during the day and night plus the summer and winter. The site typically has about 50% bare or litter covered soil, therefore soil heat transfer is more critical than in dense canopy ecosystems. The Wyoming site commonly has strong winds (5 ms-1 average) and significant daily and seasonal temperature fluctuations (as much as 30°C daily) but the warming system was nearly always able to maintain the set temperatures regardless of abiotic variation. The within canopy-air was only slightly warmed and above canopy- air was not warmed by the system, therefore convective warming was minor. Elevated CO2 had no direct effect nor interaction with the warming treatment on microclimate. Relative humidity within the plant canopy was only slightly reduced by warming. Soil water content was reduced by warming but increased by elevated CO2. This study demonstrates the importance of monitoring the microclimate in manipulative field global change experiments so that critical physiological and ecological conclusions can be determined. Highly variable energy demand fluctuations showed that passive IR heater warming systems will not maintain desired warming for much of the time

Impact of Grazing Management Strategies on Carbon Sequestration in a Semi-Arid Rangeland, USA

The effects of 12 years of grazing management strategies on carbon (C) distribution and sequestration were assessed on a semi-arid mixed-grass prairie in Wyoming, USA. Five grazing treatments were evaluated: non-grazed exclosures; continuous, season-long grazing at a light (22 steer-days ha-1) stocking rate; and, rotationally-deferred, short-duration rotation, and continuous, season-long grazing, all three at a heavy stocking rate (59 steer-days ha-1). Non-grazed exclosures exhibited a large buildup of dead plant material (72% of total aboveground plant matter) and forb biomass represented a large component (35%) of the plant community. Stocking rate, but not grazing strategy, changed plant community composition and decreased surface litter. Light grazing decreased forbs and increased cool-season mid-grasses, resulting in a highly diversified plant community and the highest total production of grasses. Heavy grazing increased warm-season grasses at the expense of the cool-season grasses, which decreased total forage production and opportunity for early season grazing. Compared to the exclosures, all grazing treatments resulted in significantly higher levels of C (6000-9000 kg ha-1) in the surface 15 cm of the soil. Higher levels of soil C with grazing are likely the result of faster litter decomposition and recycling, and redistribution of C within the 0-60 cm plant-soil system. Grazing at an appropriate stocking rate had beneficial effects on plant composition, forage production, and soil C sequestration. Without grazing, deterioration of the plant-soil system is indicated

Elevated CO\u3csub\u3e2\u3c/sub\u3e Enhances Productivity and the C/N Ratio of Grasses in the Colorado Shortgrass Steppe

Atmospheric CO2 concentrations have been increasing since the industrial revolution, and are projected to double within this century over today\u27s concentration of 360 µmol mol-1 . This study used six open-top chambers in the Colorado, USA shortgrass steppe to investigate how increasing CO2 will affect productivity and C and N status of indigenous perennial grasses and forbs. From March until October, chambers were placed on two plots in each of the three blocks. In each block, one chamber was assigned an ambient CO2 treatment (~360 µmol mol-1), the other an elevated CO2 treatment (~720 µmol mol-1). Each block also had an unchambered control plot. Growth under elevated CO2 increased above-ground phytomass an average 31% in 1997 and 47% in 1998, with no differences in relative growth responses of C3 and C4 grasses and forbs. Growth in chambers was greater than non-chambered control plots, presumably due to warmer temperatures in chambers and a longer growing season. Shoot N concentrations were reduced 21% and C/N ratios increased 23% in elevated compared to ambient chambers. Variation in aboveground phytomass due to year, CO2 and chamber effects correlated well to % shoot N and C/N ratios, although for both traits different regression lines were required for green plant material (harvested in July) and senescent plant material (harvested in October). Results suggest increased growth and reduced N concentrations in this mixed C3/C4 grassland in an elevated CO2 environment

Disentangling root responses to climate change in a semiarid grassland

Future ecosystem properties of grasslands will be driven largely by belowground biomass responses to climate change, which are challenging to understand due to experimental and technical constraints. We used a multi-faceted approach to explore single and combined impacts of elevated CO2 and warming on root carbon (C) and nitrogen (N) dynamics in a temperate, semiarid, native grassland at the Prairie Heating and CO2 Enrichment experiment. To investigate the indirect, moisture mediated effects of elevated CO2, we included an irrigation treatment. We assessed root standing mass, morphology, residence time and seasonal appearance/disappearance of community-aggregated roots, as well as mass and N losses during decomposition of two dominant grass species (a C3 and a C4). In contrast to what is common in mesic grasslands, greater root standing mass under elevated CO2 resulted from increased production, unmatched by disappearance. Elevated CO2 plus warming produced roots that were longer, thinner and had greater surface area, which, together with greater standing biomass, could potentially alter root function and dynamics. Decomposition increased under environmental conditions generated by elevated CO2, but not those generated by warming, likely due to soil desiccation with warming. Elevated CO2, particularly under warming, slowed N release from C4—but not C3—roots, and consequently could indirectly affect N availability through treatment effects on species composition. Elevated CO2 and warming effects on root morphology and decomposition could offset increased C inputs from greater root biomass, thereby limiting future net C accrual in this semiarid grassland

Disentangling root responses to climate change in a semiarid grassland

Future ecosystem properties of grasslands will be driven largely by belowground biomass responses to climate change, which are challenging to understand due to experimental and technical constraints. We used a multi-faceted approach to explore single and combined impacts of elevated CO2 and warming on root carbon (C) and nitrogen (N) dynamics in a temperate, semiarid, native grassland at the Prairie Heating and CO2 Enrichment experiment. To investigate the indirect, moisture mediated effects of elevated CO2, we included an irrigation treatment. We assessed root standing mass, morphology, residence time and seasonal appearance/disappearance of community-aggregated roots, as well as mass and N losses during decomposition of two dominant grass species (a C3 and a C4). In contrast to what is common in mesic grasslands, greater root standing mass under elevated CO2 resulted from increased production, unmatched by disappearance. Elevated CO2 plus warming produced roots that were longer, thinner and had greater surface area, which, together with greater standing biomass, could potentially alter root function and dynamics. Decomposition increased under environmental conditions generated by elevated CO2, but not those generated by warming, likely due to soil desiccation with warming. Elevated CO2, particularly under warming, slowed N release from C4—but not C3—roots, and consequently could indirectly affect N availability through treatment effects on species composition. Elevated CO2 and warming effects on root morphology and decomposition could offset increased C inputs from greater root biomass, thereby limiting future net C accrual in this semiarid grassland

Long-term exposure to elevated CO\u3csub\u3e2\u3c/sub\u3e enhances plant community stability by suppressing dominant plant species in a mixed-grass prairie

Climate controls vegetation distribution across the globe, and some vegetation types are more vulnerable to climate change, whereas others are more resistant. Because resistance and resilience can influence ecosystem stability and determine how communities and ecosystems respond to climate change, we need to evaluate the potential for resistance as we predict future ecosystem function. In a mixed-grass prairie in the northern Great Plains, we used a large field experiment to test the effects of elevated CO2, warming, and summer irrigation on plant community structure and productivity, linking changes in both to stability in plant community composition and biomass production. We show that the independent effects of CO2 and warming on community composition and productivity depend on interannual variation in precipitation and that the effects of elevated CO2 are not limited to water saving because they differ from those of irrigation. We also show that production in this mixed-grass prairie ecosystem is not only relatively resistant to interannual variation in precipitation, but also rendered more stable under elevated CO2 conditions. This increase in production stability is the result of altered community dominance patterns: Community evenness increases as dominant species decrease in biomass under elevated CO2. In many grasslands that serve as rangelands, the economic value of the ecosystem is largely dependent on plant community composition and the relative abundance of key forage species. Thus, our results have implications for how we manage native grasslands in the face of changing climate