354 research outputs found
Wave-breaking and generic singularities of nonlinear hyperbolic equations
Wave-breaking is studied analytically first and the results are compared with
accurate numerical simulations of 3D wave-breaking. We focus on the time
dependence of various quantities becoming singular at the onset of breaking.
The power laws derived from general arguments and the singular behavior of
solutions of nonlinear hyperbolic differential equations are in excellent
agreement with the numerical results. This shows the power of the analysis by
methods using generic concepts of nonlinear science
Solving Linux Upgradeability Problems Using Boolean Optimization
Managing the software complexity of package-based systems can be regarded as
one of the main challenges in software architectures. Upgrades are required on
a short time basis and systems are expected to be reliable and consistent after
that. For each package in the system, a set of dependencies and a set of
conflicts have to be taken into account. Although this problem is
computationally hard to solve, efficient tools are required. In the best
scenario, the solutions provided should also be optimal in order to better
fulfill users requirements and expectations. This paper describes two different
tools, both based on Boolean satisfiability (SAT), for solving Linux
upgradeability problems. The problem instances used in the evaluation of these
tools were mainly obtained from real environments, and are subject to two
different lexicographic optimization criteria. The developed tools can provide
optimal solutions for many of the instances, but a few challenges remain.
Moreover, it is our understanding that this problem has many similarities with
other configuration problems, and therefore the same techniques can be used in
other domains.Comment: In Proceedings LoCoCo 2010, arXiv:1007.083
Direct microcontact printing of oligonucleotides for biochip applications
BACKGROUND: A critical step in the fabrication of biochips is the controlled placement of probes molecules on solid surfaces. This is currently performed by sequential deposition of probes on a target surface with split or solid pins. In this article, we present a cost-effective procedure namely microcontact printing using stamps, for a parallel deposition of probes applicable for manufacturing biochips. RESULTS: Contrary to a previous work, we showed that the stamps tailored with an elastomeric poly(dimethylsiloxane) material did not require any surface modification to be able to adsorb oligonucleotides or PCR products. The adsorbed DNA molecules are subsequently printed efficiently on a target surface with high sub-micron resolution. Secondly, we showed that successive stamping is characterized by an exponential decay of the amount of transferred DNA molecules to the surface up the 4(th )print, then followed by a second regime of transfer that was dependent on the contact time and which resulted in reduced quality of the features. Thus, while consecutive stamping was possible, this procedure turned out to be less reproducible and more time consuming than simply re-inking the stamps between each print. Thirdly, we showed that the hybridization signals on arrays made by microcontact printing were 5 to 10-times higher than those made by conventional spotting methods. Finally, we demonstrated the validity of this microcontact printing method in manufacturing oligonucleotides arrays for mutations recognition in a yeast gene. CONCLUSION: The microcontact printing can be considered as a new potential technology platform to pattern DNA microarrays that may have significant advantages over the conventional spotting technologies as it is easy to implement, it uses low cost material to make the stamp, and the arrays made by this technology are 10-times more sensitive in term of hybridization signals than those manufactured by conventional spotting technology
Invasive pulmonary aspergillosis in chronic obstructive pulmonary disease: an emerging fungal pathogen
ABSTRACTAcute invasive pulmonary aspergillosis occurs predominantly in immunocompromised hosts, with increasing numbers of cases of invasive aspergillosis among patients with chronic obstructive pulmonary disease (COPD) being reported. Among 13 cases of invasive aspergillosis diagnosed in COPD patients admitted to the intensive care unit with acute respiratory distress, the only risk factor for invasive fungal infection was corticosteroid treatment. Invasive aspergillosis should be suspected in COPD patients receiving steroid treatment who have extensive pulmonary infiltrates. Survival depends on rapid diagnosis and early appropriate treatment. A decrease or interruption of steroid treatment should be considered as part of the overall therapeutic strategy
Spatiotemporal communication with synchronized optical chaos
We propose a model system that allows communication of spatiotemporal
information using an optical chaotic carrier waveform. The system is based on
broad-area nonlinear optical ring cavities, which exhibit spatiotemporal chaos
in a wide parameter range. Message recovery is possible through chaotic
synchronization between transmitter and receiver. Numerical simulations
demonstrate the feasibility of the proposed scheme, and the benefit of the
parallelism of information transfer with optical wavefronts.Comment: 4 pages, 5 figure
Quorum-sensing activity and related virulence factor expression in clinically pathogenic isolates of Pseudomonas aeruginosa
AbstractRespiratory isolates of Pseudomonas aeruginosa were collected from 58 critically-ill patients with ventilator-associated pneumonia. Expression of elastase and pyocyanin was assessed semi-quantitatively, while quorum-sensing activity was assessed by quantifying the levels of the autoinducers N-3-oxododecanoyl-L-homoserine lactone (C12-HSL) and N-butanoyl-L-homoserine lactone (C4-HSL). Correlations were sought between quorum-sensing activity and the expression of these two virulence factors, and all results were compared to those obtained with the laboratory reference strains PA103, a strain defective in quorum-sensing, and PAO1, a functional quorum-sensing strain. More than two-thirds of clinically pathogenic isolates had increased levels of elastase and/or pyocyanin, and high quorum-sensing activity, as assessed by autoinducer levels. However, a strong correlation between quorum-sensing activity and virulence factor production was revealed only for elastase and not for pyocyanin (C12-HSL/elastase, r = 0.7, p 2 Ă 10â9; C4-HSL/elastase, r = 0.7, p 2 Ă 10â9). These data suggest that the pathogenicity of P. aeruginosa isolates from critically-ill patients with ventilator-associated pneumonia is caused, at least in part, by an increase in elastase production regulated by quorum-sensing, while increased pyocyanin production in these isolates may be regulated predominantly by mechanisms other than quorum-sensing
Generalized Totalizer Encoding for Pseudo-Boolean Constraints
Pseudo-Boolean constraints, also known as 0-1 Integer Linear Constraints, are
used to model many real-world problems. A common approach to solve these
constraints is to encode them into a SAT formula. The runtime of the SAT solver
on such formula is sensitive to the manner in which the given pseudo-Boolean
constraints are encoded. In this paper, we propose generalized Totalizer
encoding (GTE), which is an arc-consistency preserving extension of the
Totalizer encoding to pseudo-Boolean constraints. Unlike some other encodings,
the number of auxiliary variables required for GTE does not depend on the
magnitudes of the coefficients. Instead, it depends on the number of distinct
combinations of these coefficients. We show the superiority of GTE with respect
to other encodings when large pseudo-Boolean constraints have low number of
distinct coefficients. Our experimental results also show that GTE remains
competitive even when the pseudo-Boolean constraints do not have this
characteristic.Comment: 10 pages, 2 figures, 2 tables. To be published in 21st International
Conference on Principles and Practice of Constraint Programming 201
Experimental observation of spatial antibunching of photons
We report an interference experiment that shows transverse spatial
antibunching of photons. Using collinear parametric down-conversion in a
Young-type fourth-order interference setup we show interference patterns that
violate the classical Schwarz inequality and should not exist at all in a
classical description.Comment: 4 pages, 7 figure
Polarization coupling and pattern selection in a type-II optical parametric oscillator
We study the role of a direct intracavity polarization coupling in the
dynamics of transverse pattern formation in type-II optical parametric
oscillators. Transverse intensity patterns are predicted from a stability
analysis, numerically observed, and described in terms of amplitude equations.
Standing wave intensity patterns for the two polarization components of the
field arise from the nonlinear competition between two concentric rings of
unstable modes in the far field. Close to threshold a wavelength is selected
leading to standing waves with the same wavelength for the two polarization
components. Far from threshold the competition stabilizes patterns in which two
different wavelengths coexist.Comment: 14 figure
Frequency selection by soliton excitation in nondegenerate intracavity downconversion
We show that soliton excitation in intracavity downconversion naturally
selects a strictly defined frequency difference between the signal and idler
fields. In particular, this phenomenon implies that if the signal has smaller
losses than the idler then its frequency is pulled away from the cavity
resonance and the idler frequency is pulled towards the resonance and {\em vice
versa}. The frequency selection is shown to be closely linked with the relative
energy balance between the idler and signal fields.Comment: 5 pages, 3 figures. To appear in Phys Rev Let
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