Preliminary Results Regarding the Use of Interspecific Hybridization of Sunflower with Helianthus argophyllus for Obtaining New Hybrids with Drought Tolerance, Adapted to Organic Farming

Taking into account the climatic changes expected in the future, significant shrinking of the current favourable ecological zones for sunflower is anticipated, and the transition period to that situation may be very short. The classical breeding process has a relatively long duration (7-9 years), so breeders are interested in taking advantage of some biotechnological methods (embryo rescue) for obtaining new sunflower lines with increasing tolerance to a certain stress factor

Extraction of phenological parameters from temporally smothed vegetation indices

Within the MARS Crop Yield Forecasting System (MCYFS; Royer and Genovese, 2004) of the European Commission vegetation indicators like NDVI, SAVI and fAPAR are operationally derived for daily, decadal and monthly time steps. Besides low resolution sensors as SPOT-VGT and NOAA-AVHRR, medium resolution data from TERRA/AQUA-MODIS or ENVISAT-MERIS are used at pan-European level. In case of available time-series, esp. NOAA AVHRR (since 1981) and SPOT-VGT (since 1998) difference values of the indicators (e.g. relative or absolute differences) and frequency analysis of the indicators (e.g. position in historical range or distribution) are calculated. The exploitation of the data is performed at full resolution, at grid level of the MCYFS or regional unmixed means (C-indicators) are used. Therefore a database has been set-up in order to provide the indicators based on a weighted average for each CORINE land cover class within an area of interest. The study aims to develop a strategy for an optimal use of the different sensors and thus derived indicators at different aggregation levels for the ingestion into the MCYFS. As a first step smoothing algorithms have to be applied to the time series to diminish noise effects. Therefore, existing methods as simple sliding windows, piecewise linear regression or fitting of polynomial functions are employed and compared. Thereafter the time-series analysis is performed with the aim to establish relationships between indicators profile features and the crop phenology.JRC.DDG.H.4-Monitoring agricultural resource

Review of Donor Support for the NGO Sector in Romania

The objective of this study is to estimate the size and composition of the donor community active in Romania, analyse giving strategies over the last ten years, identify future strategies for support, and propose mechanisms for regularly updating key information about donors' activities

Supporting Long-Term Archaeological Research in Southern Romania Chalcolithic Sites Using Multi-Platform UAV Mapping

Spatial data play a crucial role in archaeological research, and orthophotos, digital elevation models, and 3D models are frequently used for the mapping, documentation, and monitoring of archaeological sites. Thanks to the availability of compact and low-cost uncrewed airborne vehicles, the use of UAV-based photogrammetry matured in this field over the past two decades. More recently, compact airborne systems are also available that allow the recording of thermal data, multispectral data, and airborne laser scanning. In this article, various platforms and sensors are applied at the Chalcolithic archaeological sites in the Mostiștea Basin and Danube Valley (Southern Romania). By analysing the performance of the systems and the resulting data, insight is given into the selection of the appropriate system for the right application. This analysis requires thorough knowledge of data acquisition and data processing, as well. As both laser scanning and photogrammetry typically result in very large amounts of data, a special focus is also required on the storage and publication of the data. Hence, the objective of this article is to provide a full overview of various aspects of 3D data acquisition for UAV-based mapping. Based on the conclusions drawn in this article, it is stated that photogrammetry and laser scanning can result in data with similar geometrical properties when acquisition parameters are appropriately set. On the one hand, the used ALS-based system outperforms the photogrammetric platforms in terms of operational time and the area covered. On the other hand, conventional photogrammetry provides flexibility that might be required for very low-altitude flights, or emergency mapping. Furthermore, as the used ALS sensor only provides a geometrical representation of the topography, photogrammetric sensors are still required to obtain true colour or false colour composites of the surface

Efficient cellular and humoral immune response and production of virus-neutralizing antibodies by the Hepatitis B Virus S/preS1 16-42 antigen

Despite the availability of improved antiviral therapies, infection with Hepatitis B virus (HBV) remains a3 significant health issue, as a curable treatment is yet to be discovered. Current HBV vaccines relaying on the efficient expression of the small (S) envelope protein in yeast and the implementation of mass vaccination programs have clearly contributed to containment of the disease. However, the lack of an efficient immune response in up to 10% of vaccinated adults, the controversies regarding the seroprotection persistence in vaccine responders and the emergence of vaccine escape virus mutations urge for the development of better HBV immunogens. Due to the critical role played by the preS1 domain of the large (L) envelope protein in HBV infection and its ability to trigger virus neutralizing antibodies, including this protein in novel vaccine formulations has been considered a promising strategy to overcome the limitations of S only-based vaccines. In this work we aimed to combine relevant L and S epitopes in chimeric antigens, by inserting preS1 sequences within the external antigenic loop of S, followed by production in mammalian cells and detailed analysis of their antigenic and immunogenic properties. Of the newly designed antigens, the S/preS116–42 protein assembled in subviral particles (SVP) showed the highest expression and secretion levels, therefore, it was selected for further studies in vivo. Analysis of the immune response induced in mice vaccinated with S/preS116–42- and S-SVPs, respectively, demonstrated enhanced immunogenicity of the former and its ability to activate both humoral and cellular immune responses. This combined activation resulted in production of neutralizing antibodies against both wild-type and vaccine-escape HBV variants. Our results validate the design of chimeric HBV antigens and promote the novel S/preS1 protein as a potential vaccine candidate for administration in poor-responders to current HBV vaccines.publishedVersio

First results from stratigraphic investigation of Chiselet tell (Romania) using seismic full waveform inversion

In this paper we analyze the applicability of seismic full-waveform inversion (FWI) for investigation of tells, which are often characterized by heterogeneous and small-scale stratigraphy. Major aim is to identify location and shape of settlement layers and house remains. Furthermore, a paleochannel, which is important to understand the interaction between tell and the surrounding landscape, was found at the tell flank

Between Object and Subject

The current paper aims to reveal the potential of combining multiple approaches (techno-functional analysis, experimental archaeology, and X-ray Computed Tomography) when it comes to studying unique earthenware artefacts, such as the prehistoric human-shaped pot discovered within the tell settlement from Sultana-Malu Rosu (Romania), that belongs to the Kodjadermen-Gumelnita-Karanovo VI civilization (KGK VI) which thrived during the 5th millennium BC. This human-shaped pot, also known as ‘The Goddess of Sultana’, is an emblematic artefact that fascinates with its shape, gestures, and decoration. It was apparently made from a standard clay paste recipe and using basic forming techniques, with little care for the internal surface. This vessel also has several hidden cracks and some manipulation traces on its backside. In order to explore its relevance, our approach to this particular human-shaped pot included the use of archaeological data in correlation with other techniques in order to decipher the manufacturing process for such vessels, the possible way of using them, but also the meanings that they might have had for past human communities

Efficient cellular and humoral immune response and production of virus-neutralizing antibodies by the Hepatitis B Virus S/preS116-42 antigen

Despite the availability of improved antiviral therapies, infection with Hepatitis B virus (HBV) remains a3 significant health issue, as a curable treatment is yet to be discovered. Current HBV vaccines relaying on the efficient expression of the small (S) envelope protein in yeast and the implementation of mass vaccination programs have clearly contributed to containment of the disease. However, the lack of an efficient immune response in up to 10% of vaccinated adults, the controversies regarding the seroprotection persistence in vaccine responders and the emergence of vaccine escape virus mutations urge for the development of better HBV immunogens. Due to the critical role played by the preS1 domain of the large (L) envelope protein in HBV infection and its ability to trigger virus neutralizing antibodies, including this protein in novel vaccine formulations has been considered a promising strategy to overcome the limitations of S only-based vaccines. In this work we aimed to combine relevant L and S epitopes in chimeric antigens, by inserting preS1 sequences within the external antigenic loop of S, followed by production in mammalian cells and detailed analysis of their antigenic and immunogenic properties. Of the newly designed antigens, the S/preS116–42 protein assembled in subviral particles (SVP) showed the highest expression and secretion levels, therefore, it was selected for further studies in vivo. Analysis of the immune response induced in mice vaccinated with S/preS116–42- and S-SVPs, respectively, demonstrated enhanced immunogenicity of the former and its ability to activate both humoral and cellular immune responses. This combined activation resulted in production of neutralizing antibodies against both wild-type and vaccine-escape HBV variants. Our results validate the design of chimeric HBV antigens and promote the novel S/preS1 protein as a potential vaccine candidate for administration in poor-responders to current HBV vaccines

Paracrine signals from liver sinusoidal endothelium regulate hepatitis C virus replication

Hepatitis C virus (HCV) is a major cause of global morbidity, causing chronic liver injury that can progress to cirrhosis and hepatocellular carcinoma. The liver is a large and complex organ containing multiple cell types, including hepatocytes, sinusoidal endothelial cells (LSEC), Kupffer cells, and biliary epithelial cells. Hepatocytes are the major reservoir supporting HCV replication; however, the role of nonparenchymal cells in the viral lifecycle remains largely unexplored. LSEC secrete factors that promote HCV infection and transcript analysis identified bone morphogenetic protein 4 (BMP4) as a candidate endothelial-expressed proviral molecule. Recombinant BMP4 increased HCV replication and neutralization of BMP4 abrogated the proviral activity of LSEC-conditioned media. Importantly, BMP4 expression was negatively regulated by vascular endothelial growth factor A (VEGF-A) by way of a VEGF receptor-2 (VEGFR-2) primed activation of p38 MAPK. Consistent with our in vitro observations, we demonstrate that in normal liver VEGFR-2 is activated and BMP4 expression is suppressed. In contrast, in chronic liver disease including HCV infection where there is marked endothelial cell proliferation, we observed reduced endothelial cell VEGFR-2 activation and a concomitant increase in BMP4 expression. Conclusion: These studies identify a role for LSEC and BMP4 in HCV infection and highlight BMP4 as a new therapeutic target for treating individuals with liver disease