44 Resistenzzüchtung / Widerstandsfähigkeit gegen Schadorganismen I

44-1 - Bewertung der Feldresistenz verschiedener Winterrapssorten gegenüber Verticillium longisporum mittels quantitativer PCRClassification of winter oilseed rape resistance towards the soilborne pathogen Verticillium longisporum by quantitative PCRJessica Knüfer, Daniel Teshome Lopisso, Birger Koopmann, Andreas von Tiedemann44-2 - Identification and characterization of three putative compatibility factor genes involved in the plant – Verticillium interactionIdentifikation und Charakterisierung drei putativer Kompatibilitätsfaktoren in der Pflanze – Verticillium InteraktionRoxana Hossain, Lisa Krapoth, Dirk Schenke, Daguang Cai44-3 - Impact of cultivar resistance to Verticillium longisporum on drought stress tolerance of winter oilseed rape (Brassica napus)Einfluss der Sortenresistenz gegen Verticillium longisporum auf die Trockenstresstoleranz von Winterraps (Brassica napus)Daniel Lopisso, Jessica Knüfer, Birger Koopmann, Andreas von Tiedemann44-4 - Wirksamkeit von Majorgenen in Raps gegenüber Phoma lingam unter Berücksichtigung steigender Temperaturen und des PathotypenspektrumsEfficacy of major genes in oilseed rape against Phoma lingam with regard to rising temperatures and the population structureMark Winter, Coretta Klöppel , Fadeke Fajemisin, Birger Koopmann44-5 - Anfälligkeit von Raps -Resynthesen und -Sorten auf den Rapsstängelrüssler (Ceutorhynchus napi Gyll.) Befall – potentielle ResistenzfaktorenSusceptibility of resynthesized lines and cultivars of oilseed rape on rape stem weevil (Ceutorhynchus napi Gyll.) infestation – potential plant traits responsible for resistanceHeike Schäfer-Kösterke, Bernd Ulber44-6 - Zweijähriges Rassen-Monitoring von Exserohilum turcicum in europäischen MaisanbaugebietenTwo-year race monitoring for Exserohilum turcicum in European maize growing regionsHendrik Hanekamp, Andreas von Tiedemann, Birger Koopmann44-7 - Smart breeding und Nutzung des Genpools von Wildarten zur Verbesserung der Krankheitsresistenz von KartoffelnSmart breeding and exploitation of the genepool from wild species for the improvement of disease resistance in potatoJanine König, Marion Nachtigall, Ramona Thieme, Jörg Schubert44-8 - Neue Ansätze für eine effizientere Resistenzzüchtung bei RebenNew approaches for increasing efficieny of grapevine resistance breedingRudolf Eibach, Reinhard Töpfe

Mouse Acetylcholinesterase Enhances Neurite Outgrowth of Rat R28 Cells Through Interaction With Laminin-1

The enzyme acetylcholinesterase (AChE) terminates synaptic transmission at cholinergic synapses by hydrolyzing the neurotransmitter acetylcholine, but can also exert ‘non-classical’, morpho-regulatory effects on developing neurons such as stimulation of neurite outgrowth. Here, we investigated the role of AChE binding to laminin-1 on the regulation of neurite outgrowth by using cell culture, immunocytochemistry, and molecular biological approaches. To explore the role of AChE, we examined fiber growth of cells overexpressing different forms of AChE, and/or during their growth on laminin-1. A significant increase of neuritic growth as compared with controls was observed for neurons over-expressing AChE. Accordingly, addition of globular AChE to the medium increased total length of neurites. Co-transfection with PRIMA, a membrane anchor of AChE, led to an increase in fiber length similar to AChE overexpressing cells. Transfection with an AChE mutant that leads to the retention of AChE within cells had no stimulatory effect on neurite length. Noticeably, the longest neurites were produced by neurons overexpressing AChE and growing on laminin-1, suggesting that the AChE/laminin interaction is involved in regulating neurite outgrowth. Our findings demonstrate that binding of AChE to laminin-1 alters AChE activity and leads to increased neurite growth in culture. A possible mechanism of the AChE effect on neurite outgrowth is proposed due to the interaction of AChE with laminin-1

Recent investigations of the 0-5 geomagnetic field recorded by lava flows

Sherpa Romeo green journal (pre or post print only)We present a synthesis of paleomagnetic directional data collected from 873 lava flows at 17 different locations under the collaborative Time Averaged geomagnetic Field Initiative (TAFI). The data range from 05 Ma in age, and provide new high quality data with improved spatial coverage. Data quality at each site is measured using k, the best estimate of the Fisherian precision parameter, and its influence on inclination anomaly and VGP dispersion is evaluated by systematically excluding data with successively higher values of k. When combined with regional compilations from NW USA, SW USA, Japan, New Zealand, Hawaii, Mexico, S. Pacific and the Indian Ocean, a data set of 2283 pairs of declination and inclination data, with k>100, and VGP latitudes greater than 45 is obtained. This is a more than 7fold increase over similar quality data in the existing Global Paleomagnetic Database (GPMDB). The new data set spans 78 S to 53 N, and has sufficient temporal and spatial sampling to allow characterization of latitudinal variations in the timeaveraged field (TAF) and paleosecular variation (PSV) for the Brunhes and Matuyama epochs, and for the 0–5 Myr interval combined. PSV, as measured by dispersion of virtual geomagnetic poles, shows less latitudinal variation than predicted by current statistical PSV models. Variation of inclination anomaly with latitude is assessed using 2parameter zonal TAF models – these have axial quadrupole contributions of 2% – 4% of the axial dipole term, and axial octupole contributions of 3% – 5%. Approximately 2% of the octupole signature is likely the result of bias incurred by averaging unit vectors. The new data set provides significant improvement over previous compilations, and can contribute to a new generation of global paleomagnetic field models.Ye

PEDF and GDNF are key regulators of photoreceptor development and retinal neurogenesis in reaggregates from chick embryonic retina

Here, role(s) of pigment epithelial-derived factor (PEDF) and glial-derived neurotrophic factor (GDNF) on photoreceptor development in three-dimensional reaggregates from the retinae of the E6 chick embryo (rosetted spheroids) was investigated. Fully dispersed cells were reaggregated under serum-reduced conditions and supplemented with 50 ng/ml PEDF alone or in combination with 50 ng/ml GDNF. The spheroids were analyzed for cell growth, differentiation, and death using proliferating cell nuclear antigen, terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling, and other immunocytochemical stainings and semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) methods. PEDF strongly promoted synthesis of the messenger RNAs for blue and violet cone opsins and to a lesser extent on the red and green cone opsins. This correlated with an increase in the number of cone photoreceptors, as determined by the cone cell marker CERN906. Likewise, PEDF nearly completely inhibited rod differentiation, as detected by immunostaining with anti-rho4D2 and RT-PCR. Furthermore, PEDF accelerated proliferation of cells in the spheroids and inhibited apoptosis. As negative effects, PEDF inhibited the normal histotypic tissue formation of retinal aggregates and reduced the frequency of photoreceptor rosettes and IPL-like areas. Noticeably, supplementation of PEDF-treated cultures with GDNF reversed the effects of PEDF on spheroid morphology and on rod differentiation. This study establishes that PEDF strongly affects three-dimensional retinogenesis in vitro, most notably by inhibiting rod development and supporting proliferation and differentiation of cones, effects which are partially counteracted by GDNF

Single-Nucleotide Polymorphism Genotyping Identifies a Locally Endemic Clone of Methicillin-Resistant Staphylococcus aureus

We developed, tested, and applied a TaqMan real-time PCR assay for interrogation of three single-nucleotide polymorphisms that differentiate a clade (termed ‘t003-X’) within the radiation of methicillin-resistant Staphylococcus aureus (MRSA) ST225. The TaqMan assay achieved 98% typeability and results were fully concordant with DNA sequencing. By applying this assay to 305 ST225 isolates from an international collection, we demonstrate that clade t003-X is endemic in a single acute-care hospital in Germany at least since 2006, where it has caused a substantial proportion of infections. The strain was also detected in another hospital located 16 kilometers away. Strikingly, however, clade t003-X was not found in 62 other hospitals throughout Germany nor among isolates from other countries, and, hence, displayed a very restricted geographical distribution. Consequently, our results show that SNP-typing may be useful to identify and track MRSA clones that are specific to individual healthcare institutions. In contrast, the spatial dissemination pattern observed here had not been resolved by other typing procedures, including multilocus sequence typing (MLST), spa typing, DNA macrorestriction, and multilocus variable-number tandem repeat analysis (MLVA)

A complex interaction between glycine/NMDA receptors and serotonergic/noradrenergic antidepressants in the forced swim test in mice

Both clinical and preclinical studies demonstrate the antidepressant activity of the functional NMDA receptor antagonists. In this study, we assessed the effects of two glycine/NMDA receptor ligands, namely L-701,324 (antagonist) and d-cycloserine (a partial agonist) on the action of antidepressant drugs with different pharmacological profiles in the forced swim test in mice. Swim sessions were conducted by placing mice individually in glass cylinders filled with warmed water for 6 min. The duration of behavioral immobility during the last 4 min of the test was evaluated. The locomotor activity of mice was measured with photoresistor actimeters. L-701,324 and d-cycloserine given with reboxetine (administered in subeffective doses) did not change the behavior of animals in the forced swim test. A potentiating effect was seen when both tested glycine site ligands were given concomitantly with imipramine or fluoxetine in this test. The lesion of noradrenaline nerve terminals produced by DSP-4 neither altered the baseline activity nor influenced the antidepressant-like action of L-701,324 or d-cycloserine. The depletion of serotonin by p-CPA did not alter baseline activity in the forced swim test. However, it completely antagonized the antidepressant-like action produced by L-701,324 and d-cycloserine. Moreover, the antidepressant-like effects of imipramine, fluoxetine and reboxetine were abolished by d-serine, a full agonist of glycine/NMDA receptors. The present study demonstrates that glycine/NMDA receptor functional antagonists enhance the antidepressant-like action of serotonin, but not noradrenaline-based antidepressants and such their activity seems to depend on serotonin rather than noradrenaline pathway

A Field Guide to Pandemic, Epidemic and Sporadic Clones of Methicillin-Resistant Staphylococcus aureus

In recent years, methicillin-resistant Staphylococcus aureus (MRSA) have become a truly global challenge. In addition to the long-known healthcare-associated clones, novel strains have also emerged outside of the hospital settings, in the community as well as in livestock. The emergence and spread of virulent clones expressing Panton-Valentine leukocidin (PVL) is an additional cause for concern. In order to provide an overview of pandemic, epidemic and sporadic strains, more than 3,000 clinical and veterinary isolates of MRSA mainly from Germany, the United Kingdom, Ireland, France, Malta, Abu Dhabi, Hong Kong, Australia, Trinidad & Tobago as well as some reference strains from the United States have been genotyped by DNA microarray analysis. This technique allowed the assignment of the MRSA isolates to 34 distinct lineages which can be clearly defined based on non-mobile genes. The results were in accordance with data from multilocus sequence typing. More than 100 different strains were distinguished based on affiliation to these lineages, SCCmec type and the presence or absence of PVL. These strains are described here mainly with regard to clinically relevant antimicrobial resistance- and virulence-associated markers, but also in relation to epidemiology and geographic distribution. The findings of the study show a high level of biodiversity among MRSA, especially among strains harbouring SCCmec IV and V elements. The data also indicate a high rate of genetic recombination in MRSA involving SCC elements, bacteriophages or other mobile genetic elements and large-scale chromosomal replacements

A Field Guide to Pandemic, Epidemic and Sporadic Clones of Methicillin-Resistant Staphylococcus aureus

In recent years, methicillin-resistant Staphylococcus aureus (MRSA) have become a truly global challenge. In addition to the long-known healthcare-associated clones, novel strains have also emerged outside of the hospital settings, in the community as well as in livestock. The emergence and spread of virulent clones expressing Panton-Valentine leukocidin (PVL) is an additional cause for concern. In order to provide an overview of pandemic, epidemic and sporadic strains, more than 3,000 clinical and veterinary isolates of MRSA mainly from Germany, the United Kingdom, Ireland, France, Malta, Abu Dhabi, Hong Kong, Australia, Trinidad & Tobago as well as some reference strains from the United States have been genotyped by DNA microarray analysis. This technique allowed the assignment of the MRSA isolates to 34 distinct lineages which can be clearly defined based on non-mobile genes. The results were in accordance with data from multilocus sequence typing. More than 100 different strains were distinguished based on affiliation to these lineages, SCCmec type and the presence or absence of PVL. These strains are described here mainly with regard to clinically relevant antimicrobial resistance- and virulence-associated markers, but also in relation to epidemiology and geographic distribution. The findings of the study show a high level of biodiversity among MRSA, especially among strains harbouring SCCmec IV and V elements. The data also indicate a high rate of genetic recombination in MRSA involving SCC elements, bacteriophages or other mobile genetic elements and large-scale chromosomal replacements