Effects of Environmental Factors on the Growth, Optical Density and Biomass of the Green Algae Chlorella Vulgaris in Outdoor Conditions

Effect of environmental factors on the growth of the Chlorella vulgaris was studied. C. vulgaris was  cultivated in sterilized natural seawater enriched with F/2-Si medium. Then grow in bucket, tub and photobioreactor (PBR) in outdoor condition. The daily routine work consisted of culture checkups of optical density, biomass gains, atmosphere lux, culture lux, atmosphere temperature and culture temperature  were recorded. The highest biomass yields were (3.0 μg/ml-1) in December and (2.01 μg/ml-1) in November in PBR. The highest deviation was in atmosphere lux in time 8:30 (± 117.7) and lowest  deviation was in atmosphere temperature in time 15:00 (± 1.0499). Optical density (OD) indicated that the best growth of C. vulgaris in outdoor condition was obtained in 650 lux and also it increased with increasing amount of lux. Tub report of C. vulgaris showed different growing behaviors at the various concentration of light and at the different temperatures. Algal production in outdoor PBR is relatively inexpensive, but is only suitable for a few, fast-growing specie. Finally, this fact is noteworthy that in outdoor conditions, temperature and light have important role in growth of C. vulgaris in present study.  ©JASEMKeywords: Chlorella vulgaris, Biomass, Photobioreactor, Growth

Determination of the growth rates of Spirolina and Cheatoceros algae in urban waste sewage and their capability to deplete nitrate and phosphate content in the sewage

The application of cyanobacterial and diatom cultures for the treatment of industrial effluents has been well recognized.In this study aimed to evaluate the effect of urban sewage on growth of Spirolina plantensis and Chaetoceros muelleri. The experiment was conducted in 6 treatments as a growth medium. Result showed that in treatment 5 maximum cell densities was (565×102±237.7) at day7 thus treatment 5 has best condition for growth S. plantensis and in treatment 3 maximum cell density was (825×104±92) at day13.Treatment 5 has best condition for growth C. muelleri. Total chlorophyll a, contents (μg/1) recorded in S. Plantensi sand C. muelleri was highest at treatment 3(0.21±0.07) and treatment 4(0.23± 0.10) respectively. In present investigation, both the algal species can be good potential to growth in urban sewage. The urban sewage removal efficiency of C. muelleri was higher as compare to S.plantensis which can be recommended for phytoremediation purpose.Keywords: Spirolina; Chaetoceros;Nitrate and phosphate; Chlorophyll a;Urban sewage; Depletio

The Absorption of Nitrate and Phosphate from Urban Sewage by Blue-Green Algae (Spirolina Platensis) (An Alternative Medium) as Application for Removing the Pollution

The applications of blue green algae for the treatment of urban and industrial sewage have received more  attention during the recent years because they have the capacity to use nitrogenous compounds, ammonia and phosphates. In this study aimed to evaluate the ability of Spirolina platensis for absorption of nitrate and  phosphate from urban sewage. The experiment was conducted in 6 treatments as a growth medium. To test these predictions, we conducted a 15 days in indoor place under similar laboratory conditions at temperature (30ºC ± 2ºC), Light (2500 ± 500) Lux, pH between 8.0 and 11and with constant aeration. Regarding the culture media, a higher algal density was observed in the cultivation developed at treatment 5 (824 ±102 cell. mL-1). In our results showed higher efficiencies of phosphorus removal by S.platensis as compare to nitrate removal thus, S. platensis would be effective for nitrate removal only at relatively low concentrations and  phosphorus removal at high concentrations. Chlorophyll-a, content in treatment 3 was higher than other treatments. Kruscal-Wallis test showed a significant difference in treatments at the level of P<0.05. Thus, we recommend here that Spirolina platensis to be better candidate for absorption phosphate more than nitrate and it could be used for the removal or biotransformation urban sewage. Keywords: Blue-Green Algae, Spirolina, Nitrate, Phosphate, Sewage, Pollutio

Relative growth of the fiddler crab, Uca sindensis(Crustacea: Ocypodidae) in a subtropical mangrove in Pohl Port, Iran

The relative growth of the fiddler crab Uca sindensis in Pohl Port was studied. Ten 0.5 m^2 quadrates were randomly sampled monthly during low tide periods from October 2009 to September 2010. A total of 840 crabs, 449 (53.45%) males and 391 (46.5%) ‌ females, were sampled. The Carapace width of males ranged from 5.5 to 13.5 mm (mean ± SD) (8.92 ± 2.04), and of females from 5.0 to 11.5 mm (mean ± SD) (7.95 ± 1.34). Carapace height (CH) and Carapace length (CL), height of the major cheliped (HMC) of males, abdomen width of females (AW), major cheliped length (LMC), dactiluse (D), feeding cheliped (CHF) and merouse (M) were employed as dependent variables and carapace width (CW) as independent variable. The relationship between CW × CH was positive in males and females. The equation (Log CH=Log-4.881+4.681LogCW) for males (P<0.05) and Log CH=Log-1.882+3.299LogCW for females (P<0.05). The females showed allometric positive growth for CW × AW relation to male (Log AW= Log0.784+1.217Log CW) (P<0.05). Also four male crabs were found with two major cheliped in this study. Remarkable ontogenetic changes were observed in the allometric growth of the male major cheliped and the female abdomen, indicating that these structures are closely connected to the timing of sexual maturity. The allometric growth of U. sindensis in Pohl Port mangrove differed from other Uca populations so far studied, indicating that growth could have been influenced by environment variables such as food availability, population density, distribution of vegetation, sex ratio, soil temperature , organic matter, different of surface and sediment

INFLUENCE OF DIFFERENT ALGAL DIETS ON LARVAL GROWTH RATES IN THE MARINE SERPULIDAE POLYCHAETE WORM Spirobranchus kraussii

Utjecaj dostupnosti hrane na rast i preživljavanje ličinki Spirobranchus kraussii istraživan je hranidbom različitim vrstama algi. Ličinke S. kraussii hranjene su s četiri različite vrste morskih mikroalgi, pojedinačno i u različitim smjesama. Najbolji rast zabilježen je pri hranidbi s C. vulgaris, N. oculata, kao hrana jedne vrste i miješane smjese algi tijekom 15. dana nakon oplodnje. Smrtnost ličinki je bila niska (maks. 5%); a preživljavanje više od 95%. Ovi rezultati ukazuju na to da ličinke S. kraussii imaju sposobnost hraniti se alternativnim izvorima energije, a veličina i kvaliteta hrane mogu utjecati na njihov rast i održivost.Effects of food availability on larval growth and survival of Spirobranchus kraussii were studied by feeding larvae different algal diets. Newly hatched larvae of S. kraussii were fed four different marine microalgae species, singly and in various mixtures. The best growth was observed when fed C. vulgaris, N. oculata as a single species and mixed-algal diet during day 15 after fertilization. Mortality was low for larvae (max. 5%); survival rate more than 95%. These results suggest that S. kraussii larvae have the capacity to feed using alternative sources of energy, and food size and quality can affect their growth and sustainability

Exploring Institutional Strategies for Attaining the Objectives of the Tehran Metropolitan Plan

The extreme spatial disparity and heterogeneity between Tehran city and other settlements in its urban area have led to a decline in spatial justice and amplified differences within the Tehran metropolitan area. This study aims to identify the key factors that influence the realization of the Tehran Metropolitan Plan objectives in the metropolitan area management system using an institutional approach and meta-synthesis method. A systematic review of scientific research articles published between 1390 to 1400 was conducted, and the meta-synthesis method was used to analyze the findings of 23 studies. The results indicate that the reformation of Tehran Metropolitan's organizational structure is the most crucial step toward institutional change, as per the proposed indicators. At the variable level, the study emphasizes the need for the adoption of a multi-centered development model that aligns with institutional change in planning systems, comprehensive and integrated management of the metropolitan area, shifting towards governance of the metropolitan area, centralization, creating a multifaceted functional network, transparency, accountability, and collaborative efforts

Ontogeny of the digestive enzyme activity of the pikeperch (Sander lucioperca) under culture condition

The pikeperch (Sander lucioperca) is a species with a high potential for aquaculture and a valuable food with high market acceptance. The aim of the study was to evaluate the functional ontogeny of digestive enzyme of pikeperch from hatching to 45 days-post fertilization, 777 degree-day (DPF, dd) under culture condition. The average total length (TL) of larvae measured at hatching was 3.6 ± 0.4 mm (5 DPF; 67 dd) and at the end of experiment (45 DPF, 777 dd) was 27.1 ± 1.1 mm. The survival rate was 80-90% during the experiment period. Inhibition zimography reveals the presence of nine bands with proteolytic activity in the digestive tract of juvenile pikeperch. Zimography results during the ontogeny revealed that in larvae at 8 DPF (108 dd) and 13 DPF (189 dd), three bands were presented. The variations observed in the enzymatic activity reflected a high amount of total protease activity at 10 DPF (133.5 dd). Regarding pepsin, its activity was observed for the first time at 26 DPF (378.9 dd). Lipase activity remained constant from hatching to 26 DPF (378.9 dd). The highest amount of α-amylase activity was detected at 15 DPF (211.5 dd) and 45 DPF (777 dd). The low lipase enzyme activity suggested that live feeds with low lipid were more suitable than diets containing high lipid levels; larvae had also early capability to digest nutrient-dense diet that was high in protein. According to results the pikeperch larvae possess after the exogenous feeding, a functional digestive system with high activities that indicated the gradual development of the digestive system

Regulatory mechanisms involved in muscle and bone remodeling during refeeding in gilthead sea bream

The tolerance of fish to fasting offers a model to study the regulatory mechanisms and changes produced when feeding is restored. Gilthead sea bream juveniles were exposed to a 21-days fasting period followed by 2h to 7-days refeeding. Fasting provoked a decrease in body weight, somatic indexes, and muscle gene expression of members of the Gh/Igf system, signaling molecules (akt, tor and downstream effectors), proliferation marker pcna, myogenic regulatory factors, myostatin, and proteolytic molecules such as cathepsins or calpains, while most ubiquitin-proteasome system members increased or remained stable. In bone, downregulated expression of Gh/Igf members and osteogenic factors was observed, whereas expression of the osteoclastic marker ctsk was increased. Refeeding recovered the expression of Gh/Igf system, myogenic and osteogenic factors in a sequence similar to that of development. Akt and Tor phosphorylation raised at 2 and 5h post-refeeding, much faster than its gene expression increased, which occurred at day 7. The expression in bone and muscle of the inhibitor myostatin (mstn2) showed an inverse profile suggesting an inter-organ coordination that needs to be further explored in fish. Overall, this study provides new information on the molecules involved in the musculoskeletal system remodeling during the early stages of refeeding in fish

<span style="font-size:15.0pt;font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Mangal; mso-ansi-language:EN-GB;mso-bidi-language:HI" lang="EN-GB">Population dynamic of fiddler crab, <i>Uca</i> <i>sindensis</i> (Decapoda:Ocypodidae) in Pohl port mangrove forest, Persian Gulf, Iran </span>

2217-2222The population dynamics of Uca sindensis were investigated for the first time in Pohl port mangrove in north of Persian Gulf using Length- Frequency analysis. Nine 0.5 m2 quadrates were randomly sampled during low tide periods every month, from October 2009 to September 2010. The quadrates ran in three substrate, sand, silt and muddy flat, in order to sample of total substrate. A total of 710 crabs, 343 (47.7%) males and 367 (52.3%) females, were sampled. Sex ratio, von Bertalanffy growth parameters, recruitment pattern, growth performance and natural mortality coefficient were calculated and also Length-based stock assessment using FISAT software package. There was not different significant in sex ratio (1:1.069).The CW, Asymptotic Length(L∞) and Growth curvature (K) and φ were 3 to 14.5 mm, 17.3 mm, 0.33 year-1and1.98 in males and 4 to 12 mm, 16.28 mm0.36 year-1 and 1.94 in female, respectively. The natural mortality coefficient and was estimated to be 0.95 for males and 1.05 for females. The growth of U. sindensis follows the seasonality oscillating VBGF typical of soil temperate. Recruitment pattern shows seasonal recruitment pattern in total population. In conclusion, descriptive of life U. sindensis has a k-selection strategy that in die young, reproduce early, live last and high mortality. </span

Regulatory mechanisms involved in muscle and bone remodeling during refeeding in gilthead sea bream

The tolerance of fish to fasting offers a model to study the regulatory mechanisms and changes produced when feeding is restored. Gilthead sea bream juveniles were exposed to a 21-days fasting period followed by 2h to 7-days refeeding. Fasting provoked a decrease in body weight, somatic indexes, and muscle gene expression of members of the Gh/Igf system, signaling molecules (akt, tor and downstream effectors), proliferation marker pcna, myogenic regulatory factors, myostatin, and proteolytic molecules such as cathepsins or calpains, while most ubiquitin-proteasome system members increased or remained stable. In bone, downregulated expression of Gh/Igf members and osteogenic factors was observed, whereas expression of the osteoclastic marker ctsk was increased. Refeeding recovered the expression of Gh/Igf system, myogenic and osteogenic factors in a sequence similar to that of development. Akt and Tor phosphorylation raised at 2 and 5h post-refeeding, much faster than its gene expression increased, which occurred at day 7. The expression in bone and muscle of the inhibitor myostatin (mstn2) showed an inverse profile suggesting an inter-organ coordination that needs to be further explored in fish. Overall, this study provides new information on the molecules involved in the musculoskeletal system remodeling during the early stages of refeeding in fish