Functional analysis of responses to stress in distant prokaryotes: comparison between Mycobacterium tuberculosis and Escherichia coli

This project combines life -i.e., biological- sciences methodologies with physical and computational analyses of protein expression for two differentiated microorganisms with a completely different lifestyles: E.coli, a well-known bacteria, and M.tuberculosis, a deathly human pathogen. In other to do that, we build two folding change multilayer networks of protein expression and analyze them. The multilayer networks have six layers which are equivalent to six stress conditions: acid, cell damage wall, hypoxia, ion deprivation, oxydative stress and starvation. To do the analysis and comparison between the networks corresponding to the two bacteria, we employed several tools. Regarding bioinformatics: GEO, metasoft; softwares as R-studio, ClueGO; statistical measures like strength, overlap and partition coefficient and statistical tests such as the Mann-Whitney and Peacock tests. Our results show that the differences in lifestyles are captured by the network approach and the proposed metrics. This work could open the path to obtain further insights about protein-protein interactions and relevant challenges such as protein function determination

Decision Making in Near Zero Energy Building Refurbishment: A Technology Alternatives Ranking Tool

International audienceDecision making in the context of Near Zero Energy Building refurbishment is subjected to heterogeneous stakeholders, tools and objectives. This paper presents a methodology to facilitate stakeholders collaboration in the refurbishment processes and identifies decision support approaches to help on the main decision milestones. This methodology is supported by a prototype (user interface and algorithm) of a decision support system (DSS) that allows ranking different refurbishment technologies. The proposed DSS uses a multi criteria decision method that combines weighting and fuzzy dominances approach. The approach is illustrated with a real data set to rank insulation materials

El trabajo cooperativo

Este artículo pretende dar una visión a cerca del trabajo cooperativo en el alumnado ya que éste no sólo es un recurso muy eficaz para enseñar a los alumnos, sino que también es un contenido escolar más que los alumnos deben aprender a lo largo de su escolaridad. Por lo tanto es un aspecto que consideramos de gran importancia, el cual enfocaremos en este trabajo hacia el alumnado de secundaria para fomentar en ellos la sociabilidad e interacción entre iguales.Para que los alumnos aprendan a trabajar en equipo es muy importante que formen equipos de trabajo estables durante un tiempo considerable. Como veremos a continuación, esto lo trabajaremos a través de diferentes actividades en las que el alumnado debe adoptar una actitud positiva y de cooperación

EL TRABAJO COOPERATIVO.

This article aims to give an overview about the cooperative work among students as this is not only a very effective resource to teach students, but also a school freshest content that students must learn throughout their schooling. Therefore it is an aspect that we consider of great importance, which will focus in this paper to secondary school students to encourage them sociability and interaction among equals. To help students learn teamwork is very important to form teams of stable employment for a considerable time. As outlined below, this will work through different activities in which students must take a positive and cooperative attitude

Cuidados en un paciente tras craneotomía descompresiva por infarto en arteria cerebral media

El infarto maligno de arteria cerebral media (ACM), se estima que corresponde entre un 5% -10% del total de los infartos encefálicos; este tipo de infarto suele evolucionar con un edema cerebral postisquémico intenso, provocando un aumento de la PIC y si no se controla produciendo finalmente una herniación cerebral. Un tratamiento que ha demostrado reducir notablemente la mortalidad es la hemicraneotomía descompresiva, como comprobamos en el caso clínico. En el estudio del caso clínico observamos que, proporcionando unos cuidados específicos centrados en su patología y las complicaciones derivadas, en el ámbito hospitalario, y tras el alta a su domicilio realizamos una continuidad en los cuidados, el paciente consigue una recuperación óptima de su estado de salud.Departamento de EnfermeríaGrado en Enfermerí

Differential in vitro and in vivo effect of barley cysteine and serine protease inhibitors on phytopathogenic microorganisms

Protease inhibitors from plants have been involved in defence mechanisms against pests and pathogens. Phytocystatins and trypsin/α-amylase inhibitors are two of the best characterized protease inhibitor families in plants. In barley, thirteen cystatins (HvCPI-1 to 13) and the BTI-CMe trypsin inhibitor have been previously studied. Their capacity to inhibit pest digestive proteases, and the negative in vivo effect caused by plants expressing these inhibitors on pests support the defence function of these proteins. Barley cystatins are also able to inhibit in vitro fungal growth. However, the antifungal effect of these inhibitors in vivo had not been previously tested. Moreover, their in vitro and in vivo effect on plant pathogenous bacteria is still unknown. In order to obtain new insights on this feature, in vitro assays were made against different bacterial and fungal pathogens of plants using the trypsin inhibitor BTI-CMe and the thirteen barley cystatins. Most barley cystatins and the BTI-CMe inhibitor were able to inhibit mycelial growth but no bacterial growth. Transgenic Arabidopsis plants independently expressing the BTI-CMe inhibitor and the cystatin HvCPI-6 were tested against the same bacterial and fungal pathogens. Neither the HvCPI-6 expressing transgenic plants nor the BTI-CMe ones were more resistant to plant pathogen fungi and bacteria than control Arabidopsis plants. The differences observed between the in vitro and in planta assays against phytopathogenic fungi are discusse

Análisis en la aplicación del Plan de Acción de conservación de la Tortuga Carey Eretmochelys imbricata (Linneo 1766) en Isla Fuerte, Bolívar Caribe Colombiano

La tortuga carey (Eretmochelys imbricata) se encuentra en peligro crítico de extinción. La especie frecuenta áreas insulares del Caribe colombiano que cuenta con abundancia de hábitats que atraen a las tortugas marinas como lugares estratégicos para alimentación y anidación. En Isla Fuerte, Bolívar se han reportado anidaciones de tortuga carey y debido a su estado actual es necesario realizar esfuerzos de conservación. Actualmente existen normas ambientales para la protección y conservación de las tortugas marinas como las que soporta el Plan de Acción Nacional para la conservación de las Tortugas Marinas , a pesar de esto, no en todas las regiones caribeñas del país se ejecuta. Por esta razón el presente trabajo fue realizado con el objetivo de analizar el cumplimiento de acciones de este Plan de Acción en Isla Fuerte, Bolívar, con el fin de identificar líneas ejecutadas y no ejecutadas para generar estrategias participativas con la comunidad. Para cumplir este objetivo se contrastó multitemporalmente aspectos ecológicos de la tortuga carey en la isla.The Hawksbill sea turtle (Eretmochelys imbricata) is critically endangered. The specie frequents caribbean waters with habitat abundance which attracts them as strategic foraging and nesting areas. Some reports indicate that Isla Fuerte, Bolivar is a nesting island, that is why is important to make conservation efforts in the area. Nowadays, there is a national conservation plan for sea turtles in Colombia, which has not been implemented at all. That is the reason of the project, to identify action lines of the conservation plan implemented or not in Isla Fuerte to generate participatory strategies with the community. To achieve this objective, multitemporal contrast was necessary to know ecological aspects of the hawksbill in the island. The achieve percentage of the plan action lines was only of 8.4% and some problems show up from the social component. The priorization of the identified problems through Vester Matrix shows the lack in environmental education, in ecosystems protection and in habitat conservation.Biólogo (a)Pregrad

La Motivación en las Aulas a través de las Tecnologías de la Información y de la Comunicación

Veremos cómo las nuevas tecnologías, frente a las tradicionales, posibilitan que el maestro ponga en marcha mecanismos de creatividad, que fomenten la participación y el trabajo cooperativo, logrando una mayor interacción entre profesor y alumnos, entre estos entre sí, y en consecuencia una mayor motivación que favorece el aprendizaje