The many meanings of collective action: lessons on enhancing gender inclusion and equity in watershed management

"Collective action in agriculture and natural resource management is all too often perceived of in terms of the mere number of participants, with little consideration given to who participates, why, and the outcomes of inequitable participation. The literature is replete with cases of how uncritical approaches to participation structure positions of privilege vis-à-vis project benefits and the natural resource base. Yet lessons on how to engage with local communities in ways that promote equitable participation of women, the poor and other stakeholders are only now coming to light. This paper focuses on approaches under development under the rubric of the African Highlands Initiative to bring collective action principles to bear on gender-equitable change processes in natural resource management. The paper utilizes a number of case studies to illustrate the relative strengths and weaknesses of different approaches for enhancing gender inclusion and equity throughout the stages of problem diagnosis, planning and monitoring. The analysis suggests that an arbitrary definition of collective action is insufficient for assessing the relative strengths and weaknesses of different approaches, and that method evaluation should consider the different forms that collective action can take. A typology of different forms of collective action is proposed, and then utilized to assess the relative strengths and weaknesses of different approaches for fostering gender inclusion and equity in watershed management." Authors' AbstractNatural resource management, Gender, Water, Collective action, Community organizations, Community-based organizations, Women, Watershed management,

Catalyzing self-governance: Addressing multi-faceted collective action dilemmas in densely settled agrarian landscapes

This paper summarizes lessons learnt from efforts to pilot institutional innovations to address locally salient natural resource management challenges in the eastern African highlands, where dense human settlement and steep slopes lead to a tight coupling of interactions among adjacent land users, landholdings and property regimes. The collective action problems present in these heterogeneous landscapes include common pool resource dilemmas, as well as other “commons-like problems” involving shared or intertwined interests that cut across a range of property regimes. By creatively embedding design principles derived from long-enduring common property regimes into facilitation strategies, solutions to longstanding, relatively intractable resource management challenges (e.g. pest management, erosion) were forged through collective choice arrangements. Solutions involved not just restricting access and creating incentives for users to invest in a shared resource (e.g., through clear allocation of rights and duties), but negotiating creative solutions to match provisioning with diverse forms of benefit or to minimize/offset losses that would otherwise accrue from efforts to minimize harm to others. The paper’s contributions are threefold. First, it illustrates how self-governance is not just a feature of long-enduring common property regimes, but may be catalyzed in many situations in which it is absent. It also illustrates the applicability of Ostrom’s design principles in a complex and heterogeneous landscapes involving a diversity of natural resource forms, tenure regimes and collective action problems. Finally, it illustrates the crucial importance of bringing an institutional lens to bear on classic “technological” challenges such as soil erosion control, pest management and loss of on-farm biodiversity

Dengue Virus Genome Uncoating Requires Ubiquitination

The process of genome release or uncoating after viral entry is one of the least-studied steps in the flavivirus life cycle. Flaviviruses are mainly arthropod-borne viruses, including emerging and reemerging pathogens such as dengue, Zika, and West Nile viruses. Currently, dengue virus is one of the most significant human viral pathogens transmitted by mosquitoes and is responsible for about 390 million infections every year around the world. Here, we examined for the first time molecular aspects of dengue virus genome uncoating. We followed the fate of the capsid protein and RNA genome early during infection and found that capsid is degraded after viral internalization by the host ubiquitin-proteasome system. However, proteasome activity and capsid degradation were not necessary to free the genome for initial viral translation. Unexpectedly, genome uncoating was blocked by inhibiting ubiquitination. Using different assays to bypass entry and evaluate the first rounds of viral translation, a narrow window of time during infection that requires ubiquitination but not proteasome activity was identified. In this regard, ubiquitin E1-activating enzyme inhibition was sufficient to stabilize the incoming viral genome in the cytoplasm of infected cells, causing its retention in either endosomes or nucleocapsids. Our data support a model in which dengue virus genome uncoating requires a nondegradative ubiquitination step, providing new insights into this crucial but understudied viral process. IMPORTANCE: Dengue is the most significant arthropod-borne viral infection in humans. Although the number of cases increases every year, there are no approved therapeutics available for the treatment of dengue infection, and many basic aspects of the viral biology remain elusive. After entry, the viral membrane must fuse with the endosomal membrane to deliver the viral genome into the cytoplasm for translation and replication. A great deal of information has been obtained in the last decade regarding molecular aspects of the fusion step, but little is known about the events that follow this process, which leads to viral RNA release from the nucleocapsid. Here, we investigated the fate of nucleocapsid components (capsid protein and viral genome) during the infection process and found that capsid is degraded by the ubiquitin-proteasome system. However, in contrast to that observed for other RNA and DNA viruses, dengue virus capsid degradation was not responsible for genome uncoating. Interestingly, we found that dengue virus genome release requires a nondegradative ubiquitination step. These results provide the first insights into dengue virus uncoating and present new opportunities for antiviral intervention.Fil: Byk, Laura Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Iglesias, Nestor Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: de Maio, Federico Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Gebhard, Leopoldo German. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Rossi, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Gamarnik, Andrea Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentin

El Valor de los Datos entre ventajas y responsabilidades.

La masificación de Internet ha impactado en la aceleración de las comunicaciones y en las posibilidades de consumo y generación de información, de forma tal que en ella podemos encontrar desde los datos más triviales a herramientas críticas para la toma de decisiones